• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Mar 1990; 28(3): 438–442.
PMCID: PMC269639

Enzymatic RNA amplification of the enteroviruses.


Enteroviruses are among the most common causes of childhood infection. Current diagnostic techniques are often too slow and too insensitive to benefit the patient optimally. This report describes a modified polymerase chain reaction technique by which enteroviral RNA can be amplified, over a few hours, to a level detectable by agarose mini-gel electrophoresis or nucleic acid hybridization or both. Three oligomeric regions of great homology among the enteroviruses were identified and designated as a potential primer pair and probe. With this combination, all 11 of the enterovirus serotypes tested, representing the major subgroups of these pathogens, were successfully amplified and detected. The sensitivity and rapidity of this new assay speak to its potential clinical applicability in the diagnosis of enterovirus infections.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chonmaitree T, Menegus MA, Powell KR. The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY. JAMA. 1982 Apr 2;247(13):1843–1847. [PubMed]
  • Herrmann JE, Hendry RM, Collins MF. Factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. J Clin Microbiol. 1979 Aug;10(2):210–217. [PMC free article] [PubMed]
  • Hyypiä T, Stålhandske P, Vainionpä R, Pettersson U. Detection of enteroviruses by spot hybridization. J Clin Microbiol. 1984 Mar;19(3):436–438. [PMC free article] [PubMed]
  • Iizuka N, Kuge S, Nomoto A. Complete nucleotide sequence of the genome of coxsackievirus B1. Virology. 1987 Jan;156(1):64–73. [PubMed]
  • Impraim CC, Saiki RK, Erlich HA, Teplitz RL. Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides. Biochem Biophys Res Commun. 1987 Feb 13;142(3):710–716. [PubMed]
  • Jarvis WR, Tucker G. Echovirus type 7 meningitis in young children. Am J Dis Child. 1981 Nov;135(11):1009–1012. [PubMed]
  • Jenkins O, Booth JD, Minor PD, Almond JW. The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the Picornaviridae. J Gen Virol. 1987 Jul;68(Pt 7):1835–1848. [PubMed]
  • Lindberg AM, Stålhandske PO, Pettersson U. Genome of coxsackievirus B3. Virology. 1987 Jan;156(1):50–63. [PubMed]
  • Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. [PubMed]
  • Otto MJ, Fox MP, Fancher MJ, Kuhrt MF, Diana GD, McKinlay MA. In vitro activity of WIN 51711, a new broad-spectrum antipicornavirus drug. Antimicrob Agents Chemother. 1985 Jun;27(6):883–886. [PMC free article] [PubMed]
  • Rotbart HA, Abzug MJ, Levin MJ. Development and application of RNA probes for the study of picornaviruses. Mol Cell Probes. 1988 Mar;2(1):65–73. [PubMed]
  • Rotbart HA, Eastman PS, Ruth JL, Hirata KK, Levin MJ. Nonisotopic oligomeric probes for the human enteroviruses. J Clin Microbiol. 1988 Dec;26(12):2669–2671. [PMC free article] [PubMed]
  • Rotbart HA, Levin MJ, Murphy NL, Abzug MJ. RNA target loss during solid phase hybridization of body fluids--a quantitative study. Mol Cell Probes. 1987 Dec;1(4):347–358. [PubMed]
  • Rotbart HA, Levin MJ, Villarreal LP. Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses. J Clin Microbiol. 1984 Dec;20(6):1105–1108. [PMC free article] [PubMed]
  • Rotbart HA, Levin MJ, Villarreal LP, Tracy SM, Semler BL, Wimmer E. Factors affecting the detection of enteroviruses in cerebrospinal fluid with coxsackievirus B3 and poliovirus 1 cDNA probes. J Clin Microbiol. 1985 Aug;22(2):220–224. [PMC free article] [PubMed]
  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. [PubMed]
  • Seal LA, Jamison RM. Evidence for secondary structure within the virion RNA of echovirus 22. J Virol. 1984 May;50(2):641–644. [PMC free article] [PubMed]
  • Toyoda H, Kohara M, Kataoka Y, Suganuma T, Omata T, Imura N, Nomoto A. Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants. J Mol Biol. 1984 Apr 25;174(4):561–585. [PubMed]
  • Yolken RH, Torsch V. Enzyme-linked immunosorbent assay for the detection and identification of coxsackie B antigen in tissue cultures and clinical specimens. J Med Virol. 1980;6(1):45–52. [PubMed]
  • Yolken RH, Torsch VM. Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A. Infect Immun. 1981 Feb;31(2):742–750. [PMC free article] [PubMed]
  • Yousef GE, Brown IN, Mowbray JF. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology. 1987;28(3):163–170. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...