• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Feb 1990; 28(2): 332–335.
PMCID: PMC269601

Detection of Entamoeba histolytica immunoglobulins G and M to plasma membrane antigen by enzyme-linked immunosorbent assay.


Sixty-one serum specimens from 22 patients with clinically diagnosed amoebic liver abscess (ALA), 10 hospitalized patients with a variety of diseases other than amoebiasis, 12 normal healthy controls, and 17 subjects from an amoebiasis-endemic area were assayed by enzyme-linked immunosorbent assay (ELISA). The plasma membrane fraction of axenic cultures of Entamoeba histolytica HK9 separated from other subcellular fractions by differential centrifugation was used as the antigen to detect specific immunoglobulin G (IgG) and IgM antibodies. Using a single serum dilution of 1/100 and optical densities at 492 nm of 0.200 and 0.250 as the cutoff values for the IgM and IgG ELISAs, their respective sensitivities in 22 ALA patients were 91% (20 of 22) and 95% (21 of 22). In 22 patients (10 hospitalized and 12 normal healthy controls), the specificities of the IgM and IgG ELISAs were 95% (21 of 22) and 91% (20 of 22), respectively. All five asymptomatic carriers of pathogenic E. histolytica were seropositive by the IgG ELISA and the amoebic gel diffusion test (AGDT). The AGDT was positive for three of six culture-negative controls, while the IgG ELISA was positive for all six. For six asymptomatic carriers of nonpathogenic zymodemes, the AGDT was positive for two, and the IgG ELISA was positive for three. There was an excellent correlation (r = 0.96) between the IgG ELISA and the AGDT. Only one of six culture-negative controls, none of the asymptomatic carriers of pathogenic E. histolytica, and one of six carriers of nonpathogenic E. histolytica were seropositive by the IgM ELISA, thus highlighting the specificity of the IgM ELISA in the diagnosis of ALA. It is believed that the use of plasma membrane fractions has improved the diagnostic potential of the IgM ELISA.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (726K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aley SB, Scott WA, Cohn ZA. Plasma membrane of Entamoeba histolytica. J Exp Med. 1980 Aug 1;152(2):391–404. [PMC free article] [PubMed]
  • Bos HJ, Schouten WJ, Noordpool H, Makbin M, Oostburg BF. A seroepidemiological study of amebiasis in Surinam by the enzyme-linked immunosorbent assay (ELISA). Am J Trop Med Hyg. 1980 May;29(3):358–363. [PubMed]
  • Conradie JD, Gray R, Mbhele BE. Serum alphafetoprotein determination by enzyme-linked immunosorbent assay. S Afr Med J. 1980 Jul 26;58(4):169–171. [PubMed]
  • Conradie JD, Vorster BJ, Kirk R. A simple and rapid method of washing and drying micro-titre plates used in ELISA. J Immunoassay. 1981;2(2):109–116. [PubMed]
  • Gandhi BM, Irshad M, Acharya SK, Tandon BN. Amebic liver abscess and circulating immune complexes of Entamoeba histolytica proteins. Am J Trop Med Hyg. 1988 Nov;39(5):440–444. [PubMed]
  • Gathiram V, Jackson TF. A longitudinal study of asymptomatic carriers of pathogenic zymodemes of Entamoeba histolytica. S Afr Med J. 1987 Nov 21;72(10):669–672. [PubMed]
  • Goka AK, Rolston DD, Mathan VI, Farthing MJ. Diagnosis of giardiasis by specific IgM antibody enzyme-linked immunosorbent assay. Lancet. 1986 Jul 26;2(8500):184–186. [PubMed]
  • Jackson TF, Anderson CB, Simjee AE. Serological differentiation between past and present infection in hepatic amoebiasis. Trans R Soc Trop Med Hyg. 1984;78(3):342–345. [PubMed]
  • Jackson TF, Duursma J, Simjee AE, Pudifin DJ. Application of the leucocyte adherence inhibition test to the assessment of cell mediated immunity in invasive amoebiasis. Trans R Soc Trop Med Hyg. 1983;77(1):121–125. [PubMed]
  • Jackson TF, Gathiram V, Simjee AE. Seroepidemiological study of antibody responses to the zymodemes of Entamoeba histolytica. Lancet. 1985 Mar 30;1(8431):716–719. [PubMed]
  • Joyce MP, Ravdin JI. Antigens of Entamoeba histolytica recognized by immune sera from liver abscess patients. Am J Trop Med Hyg. 1988 Jan;38(1):74–80. [PubMed]
  • Knobloch J, Mannweiler E. Development and persistence of antibodies to Entamoeba histolytica in patients with amebic liver abscess. Analysis of 216 cases. Am J Trop Med Hyg. 1983 Jul;32(4):727–732. [PubMed]
  • Krupp IM, Powell SJ. Antibody response to invasive amebiasis in Durban, South Africa. Am J Trop Med Hyg. 1971 May;20(3):414–420. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Mathews HM, Moss DM, Visvesvara GS. Analysis of antigens from membrane and soluble fractions of Entamoeba histolytica. J Protozool. 1986 Aug;33(3):328–334. [PubMed]
  • Meza I, Cázares F, Rosales-Encina JL, Talamás-Rohana P, Rojkind M. Use of antibodies to characterize a 220-kilodalton surface protein from Entamoeba histolytica. J Infect Dis. 1987 Nov;156(5):798–805. [PubMed]
  • Mohimen A, Maitra TK, Jalan KN, Mehra S. A specific solid-phase assay for the detection of immune complexes containing Entamoeba histolytica antigens. J Immunol Methods. 1989 Feb 8;117(1):39–44. [PubMed]
  • Naot Y, Remington JS. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. J Infect Dis. 1980 Nov;142(5):757–766. [PubMed]
  • Petri WA, Jr, Joyce MP, Broman J, Smith RD, Murphy CF, Ravdin JI. Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera. Infect Immun. 1987 Oct;55(10):2327–2331. [PMC free article] [PubMed]
  • Powell SJ, Maddison SE, Wilmot AJ, Elsdon-Dew R. Amoebic gel-diffusion precipitin-test. Clinical evaluation in amoebic liver abscess. Lancet. 1965 Sep 25;2(7413):602–603. [PubMed]
  • Ravdin JI. Pathogenesis of disease caused by Entamoeba histolytica: studies of adherence, secreted toxins, and contact-dependent cytolysis. Rev Infect Dis. 1986 Mar-Apr;8(2):247–260. [PubMed]
  • Robinson GL. The laboratory diagnosis of human parasitic amoebae. Trans R Soc Trop Med Hyg. 1968;62(2):285–294. [PubMed]
  • Samrejrongroj P, Tharavanij S. Assessment of validity of counterimmunoelectrophoresis and ELISA in the routine diagnosis of amoebiasis. Southeast Asian J Trop Med Public Health. 1985 Sep;16(3):365–370. [PubMed]
  • Sathar MA, Simjee AE, Nel JD, Bredenkamp BL, Gathiram V, Jackson TF. Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess. S Afr Med J. 1988 Dec 17;74(12):625–628. [PubMed]
  • Torian BE, Lukehart SA, Stamm WE. Use of monoclonal antibodies to identify, characterize, and purify a 96,000-dalton surface antigen of pathogenic Entamoeba histolytica. J Infect Dis. 1987 Aug;156(2):334–343. [PubMed]
  • Yang J, Kennedy MT. Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis. J Clin Microbiol. 1979 Dec;10(6):778–785. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...