• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Dec 1987; 25(12): 2402–2404.
PMCID: PMC269500

Restriction endonuclease DNA analysis of Clostridium difficile.

Abstract

HindIII restriction enzyme digests of genomic DNA from nine distinct strains of Clostridium difficile were undertaken, and the results were related to those of a previously established typing method based on [35S]methionine-labeled protein profiles. Each of the typed strains identified by its protein profile could also be distinguished by its unique DNA digestion pattern. Analysis of strains isolated from 10 patients during a hospital outbreak of antibiotic-associated colitis revealed identical DNA profiles, confirming a single strain as the source of cross-infection. Characterization of isolates from worldwide sources revealed similar digestion patterns within the same strain type. Restriction endonuclease DNA analysis provides a sensitive and useful technique for studying the epidemiology of C. difficile.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (699K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bartlett JG, Chang TW, Gurwith M, Gorbach SL, Onderdonk AB. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med. 1978 Mar 9;298(10):531–534. [PubMed]
  • Bradbury WC, Pearson AD, Marko MA, Congi RV, Penner JL. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol. 1984 Mar;19(3):342–346. [PMC free article] [PubMed]
  • Delmee M, Homel M, Wauters G. Serogrouping of Clostridium difficile strains by slide agglutination. J Clin Microbiol. 1985 Mar;21(3):323–327. [PMC free article] [PubMed]
  • George RH, Symonds JM, Dimock F, Brown JD, Arabi Y, Shinagawa N, Keighley MR, Alexander-Williams J, Burdon DW. Identification of Clostridium difficile as a cause of pseudomembranous colitis. Br Med J. 1978 Mar 18;1(6114):695–695. [PMC free article] [PubMed]
  • Heard SR, O'Farrell S, Holland D, Crook S, Barnett MJ, Tabaqchali S. The epidemiology of Clostridium difficile with use of a typing scheme: nosocomial acquisition and cross-infection among immunocompromised patients. J Infect Dis. 1986 Jan;153(1):159–162. [PubMed]
  • Kuijper EJ, Oudbier JH, Stuifbergen WN, Jansz A, Zanen HC. Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea. J Clin Microbiol. 1987 Apr;25(4):751–753. [PMC free article] [PubMed]
  • Larson HE, Price AB, Honour P, Borriello SP. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet. 1978 May 20;1(8073):1063–1066. [PubMed]
  • Muldrow LL, Archibold ER, Nunez-Montiel OL, Sheehy RJ. Survey of the extrachromosomal gene pool of Clostridium difficile. J Clin Microbiol. 1982 Oct;16(4):637–640. [PMC free article] [PubMed]
  • Nakamura S, Serikawa T, Mikawa M, Nakashio S, Yamakawa K, Nishida S. Agglutination, toxigenicity and sorbitol fermentation of Clostridium difficile. Microbiol Immunol. 1981;25(9):863–870. [PubMed]
  • Pappenheimer AM, Jr, Murphy JR. Studies on the molecular epidemiology of diphtheria. Lancet. 1983 Oct 22;2(8356):923–926. [PubMed]
  • Pierce PF, Jr, Wilson R, Silva J, Jr, Garagusi VF, Rifkin GD, Fekety R, Nunez-Montiel O, Dowell VR, Jr, Hughes JM. Antibiotic-associated pseudomembranous colitis: an epidemiologic investigation of a cluster of cases. J Infect Dis. 1982 Feb;145(2):269–274. [PubMed]
  • Sell TL, Schaberg DR, Fekety FR. Bacteriophage and bacteriocin typing scheme for Clostridium difficile. J Clin Microbiol. 1983 Jun;17(6):1148–1152. [PMC free article] [PubMed]
  • Tabaqchali S, Holland D, O'Farrell S, Silman R. Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet. 1984 Apr 28;1(8383):935–938. [PubMed]
  • Tabaqchali S, O'Farrell S, Holland D, Silman R. Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins. J Clin Microbiol. 1986 Jan;23(1):197–198. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...