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Logo of bmcmicrBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Microbiology
BMC Microbiol. 2009; 9: 101.
Published online May 20, 2009. doi:  10.1186/1471-2180-9-101
PMCID: PMC2691406

A plant MinD homologue rescues Escherichia coli HL1 mutant (ΔMinDE) in the absence of MinE

Abstract

Background

In E. coli, the Min operon (MinCDE) plays a key role in determining the site of cell division. MinE oscillates from the middle to one pole or another to drive the MinCD complex to the end of the cell. The MinCD complex prevents FtsZ ring formation and the subsequent cell division at cell ends. In Arabidopsis thaliana, a homologue of MinD has been shown to be involved in the positioning of chloroplast division site.

Results

To learn whether the MinD homologue in plants is functional in bacteria, AtMinD was expressed in E. coli. Surprisingly, AtMinD can rescue the minicell phenotype of E. coli HL1 mutant (ΔMinDE) in the absence of EcMinE. This rescue requires EcMinC. AtMinD was localized to puncta at the poles of E. coli cells and puncta in chloroplasts without oscillation. AtMinD expressed in the HL1 mutant can cause a punctate localization pattern of GFP-EcMinC at cell ends. Yeast two hybrid and BiFC analysis showed that AtMinD can interact with EcMinC.

Conclusion

Similar to the MinD in Bacillus subtilis, AtMinD is localized to the polar region in E. coli and interacts with EcMinC to confine EcFtsZ polymerization and cell division at the midpoint of the cell.

Background

In Escherichia coli, proper positioning of the cell division apparatus at midpoint of the cell is mainly controlled by Min operon, which encodes MinC, MinD and MinE [1]. FtsZ, a bacteria-type cytoskeleton, self-polymerizes, marks the division site of the cell and recruits other components of the cell division apparatus [2,3]. MinD, a membrane-bound ATPase, recruits MinC to inhibit FtsZ polymerization at the non-division site [4,5]. MinE forms a dynamic ring that undergoes a repetitive cycle of movement first to one pole and then to the opposite pole in the cell [6], and induces conformational changes in membrane-bound MinD [7], which results in release of MinC and conversion of membrane-bound MinD (MinD:ATP) to cytoplasmic MinD (MinD:ADP) [7]. This highly dynamic localization cycle of Min proteins inhibits FtsZ ring formation near cell ends and forces FtsZ and many other cell division proteins to assembly at the center of the cell [8]. FtsZ and Min proteins are conserved in a wide variety of bacteria, including cyanobacteria [9].

As endosymbionts in plant cells, chloroplasts have inherited many characters from their ancestor, cyanobacteria [10]. For example, FtsZ, MinD, MinE and ARC6 are chloroplast division proteins evolved from cyanobacteria cell division proteins [9]. Besides the similarity shared with their ancestors, some new characters were gained in these proteins during evolution. The FtsZ family in Arabidopsis includes AtFtsZ1, which lacks the conserved C-terminal domain [11]; AtFtsZ2-1 and AtFtsZ2-2 [12], which are more similar to the FtsZ in cyanobacteria than other members [13]; and ARC3, which has a much less conserved GTPase domain of FtsZ and a later acquired C-terminal MORN repeat domain [14]. All these FtsZ homologues can form a ring at the chloroplast division site [15,16]. Similar to their homologues in bacteria, MinD and MinE in Arabidopsis have been shown to be involved in the positioning of the division site in chloroplasts [17-19]. Antisense suppression of AtMinD or a single mutation in AtMinD cause misplacement of the chloroplast division site in Arabidopsis [17,20]. AtMinE antagonizes the function of AtMinD [19]. Overexpression of AtMinE in Arabidopsis results in a phenotype similar to that caused by antisense suppression of AtMinD [19]. However, AtMinD has been shown to be localized to puncta in chloroplasts [20] and never been reported to oscillate. This is quite different from that of EcMinD in E. coli.

To study the function of AtMinD, we expressed it in E. coli HL1 mutant which has a deletion of EcMinD and EcMinE and a minicell phenotype [21]. Surprisingly, the mutant phenotype was complemented. Similar to the localization in chloroplasts [20], AtMinD was localized to puncta at the poles in E. coli HL1 mutant without oscillation in the absence of EcMinE. We also confirmed that AtMinD can interact with EcMinC. AtMinD may function through EcMinC by prevent FtsZ polymerization at the polar regions of the cell. Our data suggest that the cell division of E. coli can occur at the midcell with a non-oscillating Min system which includes AtMinD and EcMinC and the working mechanism of AtMinD in chloroplasts may be different from that of EcMinD in E. coli.

Results and discussion

A MinD homologue from Arabidopsis complements the minicell mutant phenotype of E. coli HL1 mutant (ΔMinDE) in the absence of MinE

The E. coli HL1 mutant (ΔMinDE) has an apparent minicell phenotype with 30.5% of the cells are shorter than 2 μm and 38.1% of the cells are between 2 μm to 5 μm (Figure (Figure1B1B and Table Table1).1). Actually, most of the cells shorter than 2 μm are minicells that are usually shorter than 1.2 μm. In the wild-type DH5α, only 2.6% of the cells are smaller than 2 μm and 97.4% of the cells are between 2 μm to 5 μm (Figure (Figure1A1A and Table Table1).1). The mutant phenotype of HL1 mutant was complemented by a pM1113-MinDE plasmid with 20 μM IPTG (Figure (Figure1C1C and Table Table1),1), which was used for the induction of MinD and MinE. Because the homologues of MinD and MinE are involved in the division of chloroplasts in plants [9] and their function may still be conserved, we set up a bacterial system to study their function. Surprisingly, a pM1113-AtMinD plasmid can complement the mutant phenotype with 50 μM IPTG in the absence of EcMinE or AtMinE (Figure (Figure1E,1E, Table Table11 and Table Table2).2). We have also grown the E. coli HL1 mutant cells (ΔMinDE) containing pM1113-AtMinD with higher or lower concentration of IPTG, and found the mutant phenotype was recovered best with 50 μM IPTG (Figure (Figure1E1E and our unpublished results). Minicells were reduced from 30.5% to 8.7% and the cells that are between 2 μm and 5 μm were increased from 38.1% to 87.4% (Table (Table1).1). Misplaced septa were also reduced from 55% to 6%, which is close to 3% in DH5α and 1% in the HL1 mutant rescued by EcMinD and EcMinE (Table (Table2).2). At higher IPTG concentration, the growth of cells was inhibited and the phenotype was not recovered so well (data not shown). Even without IPTG addition, the mutant phenotype was slightly rescued with a reduction of the cells that were 5–10 μm long from 29% to 5.6% (Table (Table1).1). This may be due to a leaky expression of AtMinD. As a control, HL1 mutant cells (ΔMinDE) transformed with a pM1113-EcMinD plasmid and grown with 20 μM IPTG showed a phenotype of long filaments but not minicells (Figure (Figure1F1F and Table Table1).1). This indicates that EcMinD is expressed and active but can not complement the mutant phenotype without EcMinE. To further understand the function of AtMinD in E. coli, AtMinD was expressed in RC1 mutant (Figure (Figure1G1G and Table Table1)1) that has a deletion of Min operon, i.e. MinCDE, with 50 μM IPTG. The RC1 mutant has a minicell phenotype similar to that of HL1 mutant. Expression of AtMinD in RC1 mutant couldn't rescue the mutant phenotype. These data suggest that the complementation of HL1 mutant by AtMinD requires the presence of EcMinC.

Table 1
Statistical analysis of the cell length
Table 2
Analysis of the cell division phenotype
Figure 1
The phenotype of E. coli cells. (A) Wildtype, DH5α. (B) HL1 mutant (ΔMinDE). (C) HL1 mutant (ΔMinDE) complemented by pM1113-MinDE at 20 μM IPTG. (D) HL1 mutant (ΔMinDE) cannot be complemented by pM1113-AtMinD at ...

The sequences of the MinD in bacteria are similar to those in plants [17]. Members of the MinD family have important roles in positioning the FtsZ ring and the division apparatus to either the mid-cell of bacteria or the mid-site of chloroplasts [9]. The complementation of E. coli HL1 mutant (ΔMinDE) by AtMinD and the requirement of EcMinC for this complementation suggest that the function of MinD is also conserved between bacteria and plants. However, this complementation doesn't require the presence of EcMinE suggests that AtMinD may have some characters different from that of EcMinD.

AtMinD is localized to puncta in E. coli and chloroplasts

To understand the function of AtMinD in E. coli, AtMinD-GFP and GFP-AtMinD were expressed in HL1 mutant (ΔMinDE) (Figure 2D, E, G and and2H).2H). Similar to AtMinD, AtMinD-GFP and GFP-AtMinD can complement the minicell phenotype of HL1 mutant (ΔMinDE) with 50 μM IPTG (Table (Table11 and Table Table2).2). However, the complementation of the phenotype by AtMinD-GFP was not as good as the complementation by AtMinD (Table (Table11 and Table Table2).2). This could be because the GFP tag partially affects the function of AtMinD-GFP. We have also tried to induce the expression of AtMinD-GFP with different concentration of IPTG (our unpublished results) and found that the mutant phenotype was complemented best with 50 μM IPTG, the same concentration as that for the complementation by AtMinD. This suggests that, although AtMinD-GFP may not be as effective as AtMinD for the complementation, both of them may interact with other division proteins with a similar stoichiometry and the interaction may not be affected by a GFP tag.

Figure 2
Localization of AtMinD in Arabidopsis and E. coli with a GFP tag. (A to C) AtMinD-GFP transiently expressed in an Arabidopsis protoplast. Arrows denote the localization of GFP in chloroplasts. (D and G) AtMinD-GFP expressed in E. coli HL1 mutant. (E and ...

In the complemented mutant cells, AtMinD-GFP and GFP-AtMinD were localized to puncta at the polar regions of the cell (Figure (Figure2D2D and and2E).2E). With a chloroplast targeting transit peptide, AtMinD-GFP fusion protein transiently expressed in Arabidopsis protoplasts was localized to puncta in chloroplasts (Figure 2A, B and and2C).2C). The green autoflorescence from chloroplasts wee dimmer than the signal from GFP (Figure (Figure2A)2A) and similar to that of untransformed cells (data not shown). This localization pattern is very similar to that of the AtMinD-GFP in stable transgenic Arabidopsis plants [19]. We have observed very carefully with time lapse images as people have done previously [22,23] for many cells with several repeats and never found the oscillation of AtMinD-GFP and GFP-AtMinD from one pole to another in the complemented E. coli HL1 mutant cells (ΔMinDE) or the chloroplasts in Arabidopsis (data not shown).

In E. coli, MinD is localized to the membrane and oscillates to one pole or another with a cytosolic protein MinC [8]. This oscillation is driven by MinE [8]. By oscillating in the cell and depolymerizing the FtsZ filaments at polar regions, the MinCD complex keeps the cell division apparatus at the midpoint of the cell [8]. Without the driver EcMinE, GFP-EcMinD was localized throughout the cell membrane with no oscillation and cells were long filaments (Figure (Figure2F2F and and2I).2I). This is probably due to a lack of FtsZ polymerization anywhere in the cell. However, a non-oscillating AtMinD can complement the phenotype of HL1 mutant (Figure (Figure1E,1E, Figure Figure2D2D and and2E2E and Table Table1).1). In this complemented mutant, MinE is also absent and AtMinD is simply localized to the polar region of the cell. Therefore, E. coli can divide at the midpoint of the cell without an oscillating Min system.

So far we don't know why AtMinD is localized to the polar region in E. coli cells. Compared with chloroplasts, E. coli cells are much smaller and have a rod shape. By just localized to the polar region, AtMinD may keep the FtsZ ring and the division site at the midpoint of the cell. Since EcMinD depolymerize the FtsZ filaments at the non-division site through its interacting protein EcMinC [8], it is also likely that AtMinD interacts with and functions through EcMinC.

To test this prediction, GFP-EcMinC and AtMinD were coexpressed at 50 μM IPTG in RC1 mutant (Figure (Figure2J2J and and2K).2K). The mutant phenotype was rescued and GFP-EcMinC was localized to puncta at cell ends except that there was some signal in the cytosol. Without AtMinD, GFP-EcMinC was distributed evenly throughout the cell in RC1 mutant (Figure (Figure2L2L and and2M).2M). These data further suggest that AtMinD may interact with EcMinC and helps interpret the complementation of HL1 mutant by AtMinD.

To get an idea of the levels of GFP-AtMinD, GFP-EcMinD and other GFP fusion proteins, an immuno-blot was done (Figure (Figure2N).2N). The levels of these proteins were very close at the same concentration of IPTG. This is probably is because their coding genes are in similar vectors and under the control of the same promoter. The level of GFP-EcMinD probably was a little higher than that of GFP-AtMinD. This could be due to a better codon usage, higher stability etc. EcMinD rescues the mutant phenotype best at 20 μM IPTG, while AtMinD and its GFP fusion proteins rescues the mutant phenotype best at 50 μM IPTG. This probably is because their working mechanisms or (and) their activities are different.

AtMinD interacts with EcMinC

To further explore the function of AtMinD, we studied the protein-protein interaction between AtMinD and EcMinC. First, we tested this by yeast two hybrid (Figure (Figure3).3). In the yeast strain AH109 we used, certain genes for the biosynthesis of histidine, leucine and tryptophan are not expressed. If two proteins fused to the bait and prey respectively interact, the genes for the synthesis of histidine, leucine and tryptophan will be induced and the yeast cell will be able to grow without histidine, leucine and tryptophan. Because this system is leaky, 3-AT was used to reduce the basal level. As shown in Figure Figure3,3, full length AtMinD can interact with EcMinC no matter whether it is fused to the activation domain or the binding domain. The presence or the absence of the chloroplast transit peptide had no effect on the interaction between AtMinD and EcMinC (Figure (Figure3).3). Both AtMinD and EcMinC can self-interact (Figure (Figure33).

Figure 3
Interactions of EcMinC and AtMinD examined by yeast two hybrid analysis. Yeast cells grown without Leucine (L), Tryptophan (T) and Histidine (H), but with 3-AT. ΔTP, deletion of the chloroplast transit peptide. SD, synthetic defined.

We also tested whether AtMinD can interact with EcMinC in chloroplasts. EcMinC fused with the N-terminal chloroplast transit peptide from Rubisco small subunit and a C-terminal GFP was transiently expressed in Arabidopsis protoplasts. Interestingly, EcMinC-GFP was localized to puncta in chloroplasts (Figure 4G, H and and4I),4I), a pattern similar to that of AtMinD-GFP in chloroplasts [20,24]. This probably is because the endogenous AtMinD has a punctate localization pattern and it can interact with EcMinC-GFP. It has been shown that overexpression of chloroplast-targeted EcMinC in plants inhibits the division of chloroplasts [25]. In E. coli, EcMinC interacts with EcMinD to be associated with membrane and to inhibit FtsZ polymerization at the polar region [8]. These data suggest that EcMinC may interact with AtMinD in chloroplasts.

Figure 4
Localization of a chloroplast-targeted EcMinC-GFP in Arabidopsis. (A to C) 35S-GFP transiently expressed in an Arabidopsis protoplast; (D to F) 35S-TP-GFP transiently expressed in Arabidopsis protoplasts; (G to I) 35S-TP-EcMinC-GFP transiently expressed ...

To further confirm the interaction between AtMinD and EcMinC, we did a BiFC analysis based on the reconstitution of YFP fluorescence when nonfluorescent N-terminal YFP (YFPN) and C-terminal YFP (YFPC) fragments are brought together by two interacting proteins in living plant cells. These two proteins were fused with a chloroplast transit peptide and a part of YFP and transiently coexpressed in Arabidopsis protoplasts (Figure (Figure5).5). AtMinD was tested by being fused with either YFPN or YFPC tag at the C-terminus for the interaction with EcMinC which has an YFPC or YFPN at the C-terminus (Figure (Figure5E5E and and5F).5F). In both cases, a strong YFP signal was detected at puncta in chloroplasts in contrast to the negative controls (Figure 5A, B and and5C).5C). It has been shown that AtMinD can self interact by FRET analysis [20] and BiFC assay [26]. Here as a positive control, AtMinD self-interacts at puncta in chloroplasts by BiFC assay (Figure (Figure5D).5D). Overall, our data strongly suggest that AtMinD can interact with EcMinC.

Figure 5
Interactions of EcMinC and AtMinD examined by BiFC assay in Arabidopsis protoplasts. (A) coexpression of 35S-YFPN and 35S-YFPC; (B) 35S-TP-EcMinC-YFPN and 35S-YFPCcoexpression; (C) 35S-AtMinD-YFPN and 35S-YFPCcoexpression; (D) 35S-AtMinD-YFPN and 35S-AtMinD-YFP ...

It is interesting that AtMinD can still recognize EcMinC. However, no MinC homologue has been found in Arabidopsis and other higher plants yet. There are at least two possibilities. First, there are a lot of differences between chloroplasts and cyanobacteria in their structure, composition and function etc. The division apparatus of chloroplasts has evolved during evolution so that MinC might have been lost in higher plants and its function has been taken by another protein. Second, the sequence of MinC is less conserved than that of MinD in bacteria (data not shown). MinC could be too divergent to be recognized by sequence in higher plants.

It is hard to understand why AtMinD is localized to static puncta in chloroplasts in previous study [20] instead of a dynamic oscillating pattern. Here we show that AtMinD is localized to puncta at the polar regions in E. coli cells (Figure (Figure2D2D and and2E)2E) and puncta in chloroplasts (Figure (Figure2A).2A). By interacting with either endogenous or transiently expressed AtMinD, EcMinC-GFP, EcMinC-YFPN and EcMinC-YFPC are localized to puncta in chloroplasts too. These data further suggest that the punctate localization pattern of AtMinD in chloroplasts shown before [20,24] may be true. There are usually only one or two GFP-labeled puncta in one chloroplast. It is possible that chloroplasts constrict in-between puncta. However, this hasn't been confirmed.

So far, it seems that the working mechanism of Min system in plastids is a lot different from that in E. coli. However, the study of Min system in plastids is limited and our understanding about it is not very clear. AtMinE seems to have an antagonistic role to AtMinD in plastid, because the chloroplast division phenotype caused by overexpression of AtMinE was similar to that caused by antisense suppression of AtMinD in Arabidopsis [17,19]. This kind of relationship is still similar to that of EcMinE and EcMinD [7]. Further study needs to be done to understand the working mechanism of AtMinE in plastids.

Conclusion

In this paper, we have shown that AtMinD was localized to puncta at the polar region and is functional in E. coli. AtMinD may function through the interaction with EcMinC. It is not necessary for AtMinD to oscillate to keep the cell division site at the center of E. coli cells. In Bacillus subtilis, the MinCD proteins are localized to polar regions without oscillation [27]. There is no MinE in B. subtilis [27]. Instead, another protein DivIVA tethers MinCD to poles of the cell and prevents FtsZ polymerization and division apparatus assembly at the end of the cells [27]. AtMinD and EcMinC in E. coli HL1 mutant (ΔMinDE) may work in a manner similar to the BsMinD and BsMinC in Bacillus subtilis.

Methods

E. coli strains and bacterial expression vector construction

The E. coli strains used in this study were DH5α, HL1 (ΔMinDE) [21] and RC1 (ΔMinCDE) [28]. The culture were grown to OD600 = 0.4 – 0.45 at 37°C in LB medium with 100 μg/ml ampicillin, 50 μg/ml kanamycin or 25 μg/ml chloramphenicol respectively as required. AtMinD lacking the coding region of the N-terminal 57 amino acid residues were amplified by using primers: AD1F1, CGGAATTCAACAAGGAATTTCTATGCCGGAACTCGCCGGAGAAACGC and AD1R1, GCAAGCTTTTAGCCGCCAAAGAAAGAGAAGA. EcMinD and EcMinDE were amplified from the genomic DNA of DH5α by primers: EcDF1, GCGGAATTCAAGGAATTTCTATGGCACG and EcDR1, GCGAAGCTTATCCTCCGAACAAGCG or EcER1, GCGAAGCTTA CAGCGGGCTTATTTCAG. These PCR products were cloned into pMLB1113 [1] between the EcoRI and HindIII restriction enzyme cutting sites to generate pM1113-AtMinD, pM1113-EcMinD and pM1113-EcMinDE. To obtain an AtMinD-GFP expression vector in E. coli, the AtMinD gene was first amplified with primers: AD1F2, CGGGATCCCATGCCGCGTATCGTCGTTATC and AD1R2, CATACCATGGTGCCGCCAAAGAAAGAGAAGA and inserted into pEGFP (Clontech, CA) between the BamHI and NcoI restriction enzyme cutting sites. Then the AtMinD-GFP fusion gene was PCR-amplified with primers AD1F1 and GFPR, CCGAAGCTTTTACTTGTACAGCTCGTC and introduced into vector pMLB1113 between the EcoRI and HindIII restriction enzyme cutting sites. To obtain GFP-AtMinD and GFP-EcMinD expression vectors, GFP was amplified from pEGFP plasmid by primers CGAATTCAACAAGGAATTTCTATGGTGAGCAAGGGC/GCTCTAGACTTGTACAGCTCGTC and cut by EcoRI and XbaI. AtMinD or EcMinD were PCR amplified by primers AD1F3, GCTCTAGAATGCCGGAACTCGCCGGAGAAACGC/AD1R1 or EcDF2, GCTCTAGAATGGCACGCATTATTGTTGT/EcDR1 and cut by XbaI and HindIII. GFP and AtMinD or EcMinD were ligated together in vitro and then inserted into pMLB1113 between EcoRI and HindIII cutting sites. For the construction of GFP-EcMinC expression vectors, EcMinC was amplified by MCF1, GCTCTAGAATGTCAAACACGCCAATCG and MCR1, ATGGATCCTCAATTTAACGGTTGAACGG and cut by XbaI and BamHI. EcMinC and the GFP gene above were ligated together in vitro and then inserted into pMLB1113 between EcoRI and BamHI cutting sites. To express AtMinD and GFP-EcMinC together, AtMinD was amplified by AD1F4, CGGGATCCAACAAGGAATTTCTATGCCGCGTATCGTCGTTATC and AD1R1, cut by BamHI and HindIII and then inserted into pMLB1113-GFP-EcMinC. All the constructs above were transformed into HL1 mutant (ΔMinDE) or RC1 mutant (ΔMinCDE) respectively.

Yeast two-hybrid analysis

AtMinD and ΔTPAtMinD were PCR-amplified with primers YDF1, GGGTTTCATATGGCGTCTCTGAGATTGTTC and YDR, CGGGATCCTTAGC CGCCAAAGAAAG or YDF2, GGGTTTCATATGCCGGAACTCGCCGGAGA AACGC and YDR, cloned into pGADT7 and pGBK (Clontech, CA, USA) which were cut by NdeI and BamHI. EcMinC was amplified with primers CF, CGGAATTCATGTCAAACACGCCAATCG and CR, ATGGATCC TCAATTTAACGGTTGAACGG, then introduced into pGADT7 and pGBK between the restriction enzyme cutting sites EcoRI and BamHI. All the constructs were first made in E. coli DH5α and then transformed into yeast strain AH109 by using the lithium acetate method. If the two proteins fused to the bait and prey respectively can interact with each other, the cotransformed yeast cells will grow in the absence of leucine, tryptophan and histidine and in the presence of 3 mM 3-AT [29-31], according to the protocol from Clontech.

AtMinD and EcMinC localization and BiFC assay in Arabidopsis

Complete open reading frame of AtMinD was amplified with gene-specific primers GCTCTAGAATGGCGTCTCTGAGATTGTTC and GCCTCGAGGCCGCCAAAGAAAGAGAAGA to remove the stop codon and have a C-terminal in-frame fusion with the coding region of GFP, YFPN (1–158 amino acid residues of YFP) and YFPC (159–239 amino acid residues of YFP). The PCR product was cut by XbaI and XhoI, and cloned into PUC19-35S-MCS-GFP, PUC19-35S-MCS-YFPN and PUC19-35S-MCS-YFPC which were constructed as previously described [32,33]. These gene manipulations generated PUC19-35S-AtMinD-GFP, PUC19-35S-AtMinD-YFPN and PUC19-35S-AtMinD-YFPC.

To obtain appropriate localization of EcMinC which had no chloroplast transit peptide, we used the first 58 amino acid residues from the Rubisco small subunit (At5g38410) in Arabidopsis thaliana. The coding region was amplified with primers TPF, GCTCTAGAGTAATGGCTTCCTCTATGCTC and TPR, GCGGATCCCTTCATGCAGCTAACTCTTCC, cloned into PUC19-35S-MCS-GFP between XbaI and BamHI cutting sites to obtain PUC19-35S-TP-GFP. EcMinC (GeneBank J03153) was PCR-amplified with primers MinCF, GCGGATCCATGTCAAACACGC CAATCG and MinCR, GCCTCGAGATTTAACGGTTGAACGGTCAAAG and cut by BamHI and XhoI and cloned into the above vector to generate PUC19-35S-TP-MinC-GFP.

The GFP gene in PUC19-35S-TP-MinC-GFP was replace with YFPN and YFPC to generate PUC19-35S-TP-MinC-YFPN and PUC19-35S-TP-MinC-YFPC.

For the localization and BiFC protein interaction analysis of AtMinD and EcMinC, the above constructs were transformed or cotransformed into Arabidopsis protoplasts by PEG-mediated method [34].

Microscopy and phenotype analysis

Differential interference contrast (DIC) microscopy and fluorescence microscopy were done by using Leica multifunctional microscope. The fluorescence in Arabidopsis protoplasts was detected by using Leica confocal laser scanning Microscope SP2. Images were processed with PHOTOSHOP software (Adobe Systems, San Jose, CA, USA).

E. coli cells in exponential growth stage and with optical density (600 nm) values between 0.4 and 0.45 were collected by centrifugation at 13 000 g for 15 minutes and the pellets were resuspended in 0.05% low melting point agar to eliminate the uneven distribution of cells on microscope slides. AxioVision AC software (Zeiss, Germany) was used to measure the size of cells. Approximately 200 cells were measured each time and three or four repeats were done. SigmaPlot 9.0 (SYSTAT Statistics, CA, USA) was used for statistical analysis of the phenotype. To score visible cell constriction sites, more than one hundred septa were counted for each genotype. Septa which were misplaced at or near a cell pole were regarded as polar septa. The percentage of polar septa for each genotype was calculated to reflect the cell division phenotype.

Immuno-blot analysis

E. coli cells were broken by ultrasonication in the extraction buffer (50 mM Tris HCl pH 8.0, 25 mM NaCl, 2 mM EDTA) and the crude total protein concentration was determined with a Dc protein assay kit (Bio-Rad). 5 μg of proteins were applied to each lane for SDS-PAGE. Immuno-blot analysis was done with polyclonal anti-GFP antibodies (Sigma, G1544).

Abbreviations

At: Arabidopsis thaliana; Ec: Escherichia coli; Bs: Bacillus subtilis; BiFC: bimolecular fluorescence complementation; GFP: green fluorescent protein; YFP: yellow fluorescent protein; 3-AT: 3-aminotriazole.

Authors' contributions

YH, HG, MZ and YH designed the experiments. MZ and JJ carried out the experiments. HG, YH, and MZ analyzed the data and wrote the paper. All authors read and approved the final manuscript.

Acknowledgements

This work was supported by NSFC Grant (No. 30470879) and PHR Grant (IHLB) to He, and NSFC Grant (No. 30500288) and STDP grant (KM200610028010) of Beijing Education Committee to Hu. We thank Dr. Lawrence Rothfield for providing the HL1 mutant (ΔMinDE), RC1 mutant (ΔMinCDE) and pMLB1113 plasmid.

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