• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Aug 1985; 22(2): 312–313.
PMCID: PMC268385

Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium.

Abstract

To isolate Mycobacterium paratuberculosis from contaminated material, a selective medium, selective Dubos medium (SDubos), was developed by supplementing conventional Dubos medium (CDubos) with carbenicillin, polymyxin, trimethoprim, and amphotericin B. The intestine and mesenteric lymph nodes of 1,501 goats were cultured in parallel on SDubos and CDubos after decontamination with oxalic acid. The contamination rate was reduced more than 150 times by the use of SDubos. The number of positive specimens (18, or 1.2%), i.e., those which revealed growth of M. paratuberculosis, was too small to evaluate the number of specimens which could have been missed due to contamination on CDubos. However, the number of specimens positive on SDubos (16, or 89%) showed that the antibiotics used were not harmful to M. paratuberculosis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (432K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chiodini RJ, Van Kruiningen HJ, Merkal RS. Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet. 1984 Jul;74(3):218–262. [PubMed]
  • Fodstad FH, Gunnarsson E. Post-mortem examination in the diagnosis of Johne's disease in goats. Acta Vet Scand. 1979;20(2):157–167. [PubMed]
  • Jørgensen JB. An improved medium for culture of Mycobacterium paratuberculosis from bovine faeces. Acta Vet Scand. 1982;23(3):325–335. [PubMed]
  • Matajack ML, Bissett ML, Schifferle D, Wood RM. Evaluation of a selective medium for mycobacteria. Am J Clin Pathol. 1973 Mar;59(3):391–397. [PubMed]
  • McClatchy JK, Waggoner RF, Kanes W, Cernich MS, Bolton TL. Isolation of mycobacteria from clinical specimens by use of selective 7H11 medium. Am J Clin Pathol. 1976 Mar;65(3):412–415. [PubMed]
  • Mitchison DA, Allen BW, Carrol L, Dickinson JM, Aber VR. A selective oleic acid albumin agar medium for tubercle bacilli. J Med Microbiol. 1972 May;5(2):165–175. [PubMed]
  • Riemann HP, Abbas B. Diagnosis and control of bovine paratuberculosis (Johne's disease). Adv Vet Sci Comp Med. 1983;27:481–506. [PubMed]
  • Rothlauf MV, Brown GL, Blair EB. Isolation of mycobacteria from undecontaminated specimens with selective 7H10 medium. J Clin Microbiol. 1981 Jan;13(1):76–79. [PMC free article] [PubMed]
  • Saxegaard F, Fodstad FH. Control of paratuberculosis (Johne's disease) in goats by vaccination. Vet Rec. 1985 Apr 20;116(16):439–441. [PubMed]
  • SMITH HW. Modifications of Dubos's media for the cultivation of Mycobacterium johnei. J Pathol Bacteriol. 1953 Oct;66(2):375–381. [PubMed]
  • Songer JG. Methods for selective isolation of mycobacteria from the environment. Can J Microbiol. 1981 Jan;27(1):1–7. [PubMed]
  • STUART P. VACCINATION AGAINST JOHNE'S DISEASE IN CATTLE EXPOSED TO EXPERIMENTAL INFECTION. Br Vet J. 1965 Jul;121:289–318. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...