• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Apr 1990; 28(4): 738–741.
PMCID: PMC267786

Rapid detection of respiratory syncytial virus in nasopharyngeal specimens obtained with the rhinoprobe scraper.


We compared the Rhinoprobe scraping technique for collection of superficial nasal mucosa epithelial cells and rapid detection of respiratory syncytial virus by immunofluorescence with paired, swab-collected specimens for virus culture from 1,257 infants and children with acute respiratory infections. Compared with viral culture as the reference test, the sensitivity, specificity, and accuracy of the immunofluorescence test were 83.6, 93.6, and 91.3%, respectively. We found the Rhinoprobe method safe, easy to use, and helpful in obtaining large quantities of epithelial cells for detection of respiratory syncytial virus and other respiratory viruses.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (761K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ahluwalia G, Embree J, McNicol P, Law B, Hammond GW. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 May;25(5):763–767. [PMC free article] [PubMed]
  • Bell DM, Walsh EE, Hruska JF, Schnabel KC, Hall CB. Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983 Jun;17(6):1099–1101. [PMC free article] [PubMed]
  • Cheeseman SH, Pierik LT, Leombruno D, Spinos KE, McIntosh K. Evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions. J Clin Microbiol. 1986 Jul;24(1):155–156. [PMC free article] [PubMed]
  • Freymuth F, Quibriac M, Petitjean J, Amiel ML, Pothier P, Denis A, Duhamel JF. Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. J Clin Microbiol. 1986 Dec;24(6):1013–1016. [PMC free article] [PubMed]
  • Gray KG, MacFarlane DE, Sommerville RG. Direct immunofluorescent identification of respiratory syncytial virus in throat swabs from children with respiratory illness. Lancet. 1968 Mar 2;1(7540):446–448. [PubMed]
  • Hall CB, Douglas RG., Jr Clinically useful method for the isolation of respiratory syncytial virus. J Infect Dis. 1975 Jan;131(1):1–5. [PubMed]
  • Hendry RM, Pierik LT, McIntosh K. Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. J Clin Microbiol. 1986 Feb;23(2):383–384. [PMC free article] [PubMed]
  • Jalowayski AA, England BL, Temm CJ, Nunemacher TJ, Bastian JF, MacPherson GA, Dankner WM, Straube RC, Connor JD. Peroxidase-antiperoxidase assay for rapid detection of respiratory syncytial virus in nasal epithelial specimens from infants and children. J Clin Microbiol. 1987 Apr;25(4):722–725. [PMC free article] [PubMed]
  • Kaul A, Scott R, Gallagher M, Scott M, Clement J, Ogra PL. Respiratory syncytial virus infection. Rapid diagnosis in children by use of indirect immunofluorescence. Am J Dis Child. 1978 Nov;132(11):1088–1090. [PubMed]
  • Lauer BA. Comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity. J Clin Microbiol. 1982 Aug;16(2):411–412. [PMC free article] [PubMed]
  • Minnich L, Ray CG. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. [PMC free article] [PubMed]
  • Shen JT, Nalick RH, Schlaerth JB, Morrow CP. Efficacy of cotton-tipped applicators for obtaining cells from the uterine cervix for Papanicolaou smears. Acta Cytol. 1984 Sep-Oct;28(5):541–545. [PubMed]
  • Treuhaft MW, Soukup JM, Sullivan BJ. Practical recommendations for the detection of pediatric respiratory syncytial virus infections. J Clin Microbiol. 1985 Aug;22(2):270–273. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...