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PMC full text:
Nature. Author manuscript; available in PMC 2010 Apr 30.
Published in final edited form as:
Nature. 2009 Apr 30; 458(7242): 1131–1135.
Published online 2009 Apr 1. doi:  10.1038/nature07976

Figure 4

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Effects of starvation, HFD feeding and a hepatocyte-specific blockage of autophagy on hepatic lipid accumulation

a, Immunoblots of cellular homogenates (Hom) and LDs from fed (F) or 24-h starved (S) mice. IκB, inhibitor of the nuclear factor of kappa light polypeptide gene enhancer; GPDH, glyceraldehyde-3-phosphate dehydrogenase. b, Percentages of autophagic vacuoles (AVs) containing only lipid, other cargo or mixed cargo calculated from samples processed as in Supplementary Fig. 16b (*P < 0.001, **P < 0.0001, n = 4–6). c, Immunoblots of Hom, AV and lysosomes (Lys). L-1, LAMP1. d, Immunoblots of liver Hom and LDs from HFD-fed mice. e, Liver homogenate immunoblots. PDI, protein disulphide isomerase. f, Total hepatic TG and cholesterol content (*P < 0.01, **P < 0.00001, n = 8–17). g, Hepatic TG concentration (*P < 0.05, n = 3). h, Percentage of cellular cholesterol in lysosomes (*P < 0.02, n = 4). i, Immunoblots of homogenates and isolated LDs. Error bars, s.e.m.

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