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J Clin Microbiol. Jun 1989; 27(6): 1387–1389.
PMCID: PMC267564

Nasopharyngeal swabs and nasopharyngeal aspirates equally effective for the diagnosis of viral respiratory disease in hospitalized children.

Abstract

Paired nasopharyngeal swab and nasopharyngeal aspirate specimens from 125 patients were compared for viral diagnosis. The viral isolation rates were comparable for the two types of specimens. There was a high level of agreement between the two specimens in overall positivity rate by immunofluorescence and positivity in culture-confirmed patients.

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Selected References

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  • Ahluwalia G, Embree J, McNicol P, Law B, Hammond GW. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 May;25(5):763–767. [PMC free article] [PubMed]
  • Arens MQ, Swierkosz EM, Schmidt RR, Armstrong T, Rivetna KA. Enhanced isolation of respiratory syncytial virus in cell culture. J Clin Microbiol. 1986 Apr;23(4):800–802. [PMC free article] [PubMed]
  • Bell DM, Walsh EE, Hruska JF, Schnabel KC, Hall CB. Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983 Jun;17(6):1099–1101. [PMC free article] [PubMed]
  • Bromberg K, Tannis G, Daidone B, Clarke L, Sierra MF. Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985 Dec;22(6):1071–1072. [PMC free article] [PubMed]
  • Mahony JB, Chernesky MA. Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens. J Clin Microbiol. 1985 Nov;22(5):865–867. [PMC free article] [PubMed]
  • Crane LR, Gutterman PA, Chapel T, Lerner AM. Incubation of swab materials with herpes simplex virus. J Infect Dis. 1980 Apr;141(4):531–531. [PubMed]
  • Cradock-Watson JE, McQuillin J, Gardner PS. Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique. J Clin Pathol. 1971 May;24(4):308–312. [PMC free article] [PubMed]
  • Cruz JR, Quiñonez E, de Fernandez A, Peralta F. Isolation of viruses from nasopharyngeal secretions: comparison of aspiration and swabbing as means of sample collection. J Infect Dis. 1987 Aug;156(2):415–416. [PubMed]
  • Gardner PS, McQuillin J, McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. [PMC free article] [PubMed]
  • Gilbert BE, Knight V. Biochemistry and clinical applications of ribavirin. Antimicrob Agents Chemother. 1986 Aug;30(2):201–205. [PMC free article] [PubMed]
  • Hall CB, Douglas RG., Jr Clinically useful method for the isolation of respiratory syncytial virus. J Infect Dis. 1975 Jan;131(1):1–5. [PubMed]
  • Hall CB, McBride JT, Walsh EE, Bell DM, Gala CL, Hildreth S, Ten Eyck LG, Hall WJ. Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983 Jun 16;308(24):1443–1447. [PubMed]
  • Hendry RM, Pierik LT, McIntosh K. Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. J Clin Microbiol. 1986 Feb;23(2):383–384. [PMC free article] [PubMed]
  • Hughes JH, Mann DR, Hamparian VV. Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. J Clin Microbiol. 1988 Mar;26(3):588–591. [PMC free article] [PubMed]
  • Kim HW, Wyatt RG, Fernie BF, Brandt CD, Arrobio JO, Jeffries BC, Parrott RH. Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. J Clin Microbiol. 1983 Dec;18(6):1399–1404. [PMC free article] [PubMed]
  • Masters HB, Weber KO, Groothuis JR, Wren CG, Lauer BA. Comparison of nasopharyngeal washings and swab specimens for diagnosis of respiratory syncytial virus by EIA, FAT, and cell culture. Diagn Microbiol Infect Dis. 1987 Oct;8(2):101–105. [PubMed]
  • McIntosh K, Hendry RM, Fahnestock ML, Pierik LT. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982 Aug;16(2):329–333. [PMC free article] [PubMed]
  • Treuhaft MW, Soukup JM, Sullivan BJ. Practical recommendations for the detection of pediatric respiratory syncytial virus infections. J Clin Microbiol. 1985 Aug;22(2):270–273. [PMC free article] [PubMed]

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