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J Clin Microbiol. Dec 1993; 31(12): 3305–3309.
PMCID: PMC266409

Karyotyping of Cryptococcus neoformans as an epidemiological tool.

Abstract

Karyotyping of Cryptococcus neoformans var. neoformans can be used as an epidemiological tool for C. neoformans infections. In this study of over 40 isolates from both clinical and environmental sources, 90% had a unique chromosome banding by pulsed-field electrophoresis. There was no conserved pattern associated with body site of infection, geographical location of the isolate, or human immunodeficiency virus status. Karyotypes of individual isolates remained stable during both in vitro passage and in vivo infections. Karyotype was used to exclude the possibility of nosocomial spread of C. neoformans in one clinical situation and supported relapse in two other cases. Because of its variable sizes between isolates, karyotyping of C. neoformans is a convenient method for molecular identification of different strains.

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  • Asakura K, Iwaguchi S, Homma M, Sukai T, Higashide K, Tanaka K. Electrophoretic karyotypes of clinically isolated yeasts of Candida albicans and C. glabrata. J Gen Microbiol. 1991 Nov;137(11):2531–2538. [PubMed]
  • Corcoran LM, Forsyth KP, Bianco AE, Brown GV, Kemp DJ. Chromosome size polymorphisms in Plasmodium falciparum can involve deletions and are frequent in natural parasite populations. Cell. 1986 Jan 17;44(1):87–95. [PubMed]
  • Kwon-Chung KJ, Wickes BL, Merz WG. Association of electrophoretic karyotype of Candida stellatoidea with virulence for mice. Infect Immun. 1988 Jul;56(7):1814–1819. [PMC free article] [PubMed]
  • Kwon-Chung KJ, Wickes BL, Stockman L, Roberts GD, Ellis D, Howard DH. Virulence, serotype, and molecular characteristics of environmental strains of Cryptococcus neoformans var. gattii. Infect Immun. 1992 May;60(5):1869–1874. [PMC free article] [PubMed]
  • Magee BB, Magee PT. Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol. 1987 Feb;133(2):425–430. [PubMed]
  • McConkey GA, Waters AP, McCutchan TF. The generation of genetic diversity in malaria parasites. Annu Rev Microbiol. 1990;44:479–498. [PubMed]
  • Merz WG, Connelly C, Hieter P. Variation of electrophoretic karyotypes among clinical isolates of Candida albicans. J Clin Microbiol. 1988 May;26(5):842–845. [PMC free article] [PubMed]
  • Meyer W, Mitchell TG, Freedman EZ, Vilgalys R. Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J Clin Microbiol. 1993 Sep;31(9):2274–2280. [PMC free article] [PubMed]
  • Min KH, Kwon-Chung KJ. The biochemical basis for the distinction between the two Cryptococcus neoformans varieties with CGB medium. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Jul;261(4):471–480. [PubMed]
  • Pagès M, Bastien P, Veas F, Rossi V, Bellis M, Wincker P, Rioux JA, Roizès G. Chromosome size and number polymorphisms in Leishmania infantum suggest amplification/deletion and possible genetic exchange. Mol Biochem Parasitol. 1989 Sep;36(2):161–168. [PubMed]
  • Pan S, Cole GT. Electrophoretic karyotypes of clinical isolates of Coccidioides immitis. Infect Immun. 1992 Nov;60(11):4872–4880. [PMC free article] [PubMed]
  • Pardue ML. Dynamic instability of chromosomes and genomes. Cell. 1991 Aug 9;66(3):427–431. [PubMed]
  • Perfect JR, Lang SD, Durack DT. Chronic cryptococcal meningitis: a new experimental model in rabbits. Am J Pathol. 1980 Oct;101(1):177–194. [PMC free article] [PubMed]
  • Perfect JR, Magee BB, Magee PT. Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun. 1989 Sep;57(9):2624–2627. [PMC free article] [PubMed]
  • Polacheck I, Lebens G, Hicks JB. Development of DNA probes for early diagnosis and epidemiological study of cryptococcosis in AIDS patients. J Clin Microbiol. 1992 Apr;30(4):925–930. [PMC free article] [PubMed]
  • Polacheck I, Lebens GA. Electrophoretic karyotype of the pathogenic yeast Cryptococcus neoformans. J Gen Microbiol. 1989 Jan;135(1):65–71. [PubMed]
  • Spitzer ED, Spitzer SG. Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans. J Clin Microbiol. 1992 May;30(5):1094–1097. [PMC free article] [PubMed]
  • Varma A, Edman JC, Kwon-Chung KJ. Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans. Infect Immun. 1992 Mar;60(3):1101–1108. [PMC free article] [PubMed]
  • Varma A, Kwon-Chung KJ. Restriction fragment polymorphism in mitochondrial DNA of Cryptococcus neoformans. J Gen Microbiol. 1989 Dec;135(12):3353–3362. [PubMed]
  • Varma A, Kwon-Chung KJ. DNA probe for strain typing of Cryptococcus neoformans. J Clin Microbiol. 1992 Nov;30(11):2960–2967. [PMC free article] [PubMed]
  • Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990 Aug;172(8):4238–4246. [PMC free article] [PubMed]
  • Wickes BL, Golin JE, Kwon-Chung KJ. Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype. Infect Immun. 1991 May;59(5):1762–1771. [PMC free article] [PubMed]

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