• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Apr 1988; 26(4): 662–667.
PMCID: PMC266403

Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera.

Abstract

Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG). Of 137 serum samples tested, none was mistyped by either WBA or IEA. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Arvin AM, Koropchak CM, Yeager AS, Pereira L. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Infect Immun. 1983 Apr;40(1):184–189. [PMC free article] [PubMed]
  • Ashley RL, Militoni J. Use of densitometric analysis for interpreting HSV serologies based on Western blot. J Virol Methods. 1987 Nov;18(2-3):159–168. [PubMed]
  • Ashley R, Benedetti J, Corey L. Humoral immune response to HSV-1 and HSV-2 viral proteins in patients with primary genital herpes. J Med Virol. 1985 Oct;17(2):153–166. [PubMed]
  • Bernstein DI, Lovett MA, Bryson YJ. Serologic analysis of first-episode nonprimary genital herpes simplex virus infection. Presence of type 2 antibody in acute serum samples. Am J Med. 1984 Dec;77(6):1055–1060. [PubMed]
  • Corey L, Nahmias AJ, Guinan ME, Benedetti JK, Critchlow CW, Holmes KK. A trial of topical acyclovir in genital herpes simplex virus infections. N Engl J Med. 1982 Jun 3;306(22):1313–1319. [PubMed]
  • Lee FK, Coleman RM, Pereira L, Bailey PD, Tatsuno M, Nahmias AJ. Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. J Clin Microbiol. 1985 Oct;22(4):641–644. [PMC free article] [PubMed]
  • Lee FK, Pereira L, Griffin C, Reid E, Nahmias A. A novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. J Virol Methods. 1986 Sep;14(2):111–118. [PubMed]
  • Lehtinen M, Koivisto V, Lehtinen T, Paavonen J, Leinikki P. Immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. Intervirology. 1985;24(1):18–25. [PubMed]
  • Marsden HS, Buckmaster A, Palfreyman JW, Hope RG, Minson AC. Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2. J Virol. 1984 May;50(2):547–554. [PMC free article] [PubMed]
  • McClung H, Seth P, Rawls WE. Relative concentrations in human sera of antibodies to cross-reacting and specific antigens of Herpes simplex virus types 1 and 2. Am J Epidemiol. 1976 Aug;104(2):192–201. [PubMed]
  • Nahmias AJ, Josey WE, Naib ZM, Luce CF, Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. [PubMed]
  • Peterson E, Schmidt OW, Goldstein LC, Nowinski RC, Corey L. Typing of clinical herpes simplex virus isolates with mouse monoclonal antibodies to herpes simplex virus types 1 and 2: comparison with type-specific rabbit antisera and restriction endonuclease analysis of viral DNA. J Clin Microbiol. 1983 Jan;17(1):92–96. [PMC free article] [PubMed]
  • Plummer G. A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera. Cancer Res. 1973 Jun;33(6):1469–1476. [PubMed]
  • Roizman B, Norrild B, Chan C, Pereira L. Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart. Virology. 1984 Feb;133(1):242–247. [PubMed]
  • Sherlock CH, Ashley RL, Shurtleff ML, Mack KD, Corey L. Type specificity of complement-fixing antibody against herpes simplex virus type 2 AG-4 early antigen in patients with asymptomatic infection. J Clin Microbiol. 1986 Dec;24(6):1093–1097. [PMC free article] [PubMed]
  • Smith JW, Adam E, Melnick JL, Rawls WE. Use of the 51 Cr release test to demonstrate patterns of antibody response in humans to herpesvirus types 1 and 2. J Immunol. 1972 Sep;109(3):554–564. [PubMed]
  • Su HK, Eberle R, Courtney RJ. Processing of the herpes simplex virus type 2 glycoprotein gG-2 results in secretion of a 34,000-Mr cleavage product. J Virol. 1987 May;61(5):1735–1737. [PMC free article] [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...