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J Clin Microbiol. Apr 1987; 25(4): 637–640.
PMCID: PMC266050

Detection of Breda virus antigen and antibody in humans and animals by enzyme immunoassay.


Enzyme immunoassays were developed for the detection of Breda virus antibody and antigen. Cattle sera collected in the United Kingdom were found to have a high prevalence of antibody (55%) to Breda virus when examined in a competitive enzyme-linked immunosorbent assay. A low prevalence of antibody was found in pigs (2.2%), and no antibody was found in sheep or goat sera. No antibody to either Breda virus or Berne virus was detected in human sera collected from veterinarians and farm workers. Only 1 of 430 human fecal specimens (0.2%) contained Breda virus antigen detectable by enzyme-linked immunosorbent assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Beards GM, Brown DW, Green J, Flewett TH. Preliminary characterisation of torovirus-like particles of humans: comparison with Berne virus of horses and Breda virus of calves. J Med Virol. 1986 Sep;20(1):67–78. [PubMed]
  • Beards GM, Hall C, Green J, Flewett TH, Lamouliatte F, Du Pasquier P. An enveloped virus in stools of children and adults with gastroenteritis that resembles the Breda virus of calves. Lancet. 1984 May 12;1(8385):1050–1052. [PubMed]
  • Horzinek MC, Ederveen J, Weiss M. The nucleocapsid of Berne virus. J Gen Virol. 1985 Jun;66(Pt 6):1287–1296. [PubMed]
  • Horzinek MC, Weiss M. Toroviridae: a taxonomic proposal. Zentralbl Veterinarmed B. 1984 Oct;31(9):649–659. [PubMed]
  • Horzinek MC, Weiss M, Ederveen J. Berne virus is not 'coronavirus-like'. J Gen Virol. 1984 Mar;65(Pt 3):645–649. [PubMed]
  • Weiss M, Steck F, Horzinek MC. Purification and partial characterization of a new enveloped RNA virus (Berne virus). J Gen Virol. 1983 Sep;64(Pt 9):1849–1858. [PubMed]
  • Weiss M, Steck F, Kaderli R, Horzinek MC. Antibodies to Berne virus in horses and other animals. Vet Microbiol. 1984 Oct;9(6):523–531. [PubMed]
  • Woode GN, Reed DE, Runnels PL, Herrig MA, Hill HT. Studies with an unclassified virus isolated from diarrheic calves. Vet Microbiol. 1982 Jul;7(3):221–240. [PubMed]
  • Woode GN, Saif LJ, Quesada M, Winand NJ, Pohlenz JF, Gourley NK. Comparative studies on three isolates of Breda virus of calves. Am J Vet Res. 1985 May;46(5):1003–1010. [PubMed]
  • Yolken RH, Leister FJ, Whitcomb LS, Santosham M. Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex. J Immunol Methods. 1983 Feb 11;56(3):319–327. [PubMed]
  • Yolken RH, Wyatt RG, Barbour BA, Kim HW, Kapikian AZ, Chanock RM. Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay. J Clin Microbiol. 1978 Sep;8(3):283–287. [PMC free article] [PubMed]

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