• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Oct 1993; 31(10): 2764–2768.
PMCID: PMC266009

Growth of 28 Legionella species on selective culture media: a comparative study.


We compared the growth of 28 Legionella spp. on four manufacturers' buffered charcoal-yeast extract (BCYE) agar media and selective BCYE media that contained polymyxin B, anisomycin, and vancomycin or cefamandole. With BCYE as a "gold standard," growth for Legionella pneumophila was significantly better than for the nonpneumophila species on all media tested. L. pneumophila and 24 other Legionella spp. grew on vancomycin-containing media, while L. santicrucis, L. rubrilucens, and L. erythra grew poorly. In contrast, 11 of 28 species (notably L. micdadei and L. bozemanii) did not grow on cefamandole-containing media and 8 of 28 species only grew marginally. We demonstrated that selective BCYE media that contain vancomycin or cefamandole may not support the growth of all Legionella spp. One commercial manufacturer's media were consistently suboptimal. Laboratories should not rely on a manufacturer's quality control testing in lieu of their own.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (859K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brown A, Shonnard JW, Geyer SJ, Rihs J, Elder E, Stout J, Vickers R, Yu V. Coincident infection with legionnaires' disease bacterium and Pittsburgh pneumonia agent. Lancet. 1981 Nov 7;2(8254):1041–1041. [PubMed]
  • Edelstein PH, Edelstein MA. Comparison of different agars used in the formulation of buffered charcoal yeast extract medium. J Clin Microbiol. 1991 Jan;29(1):190–191. [PMC free article] [PubMed]
  • Fang GD, Yu VL, Vickers RM. Disease due to the Legionellaceae (other than Legionella pneumophila). Historical, microbiological, clinical, and epidemiological review. Medicine (Baltimore) 1989 Mar;68(2):116–132. [PubMed]
  • Feeley JC, Gibson RJ, Gorman GW, Langford NC, Rasheed JK, Mackel DC, Baine WB. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. [PMC free article] [PubMed]
  • Morrill WE, Barbaree JM, Fields BS, Sanden GN, Martin WT. Increased recovery of Legionella micdadei and Legionella bozemanii on buffered charcoal yeast extract agar supplemented with albumin. J Clin Microbiol. 1990 Mar;28(3):616–618. [PMC free article] [PubMed]
  • Stout JE, Best MG, Yu VL. Susceptibility of members of the family Legionellaceae to thermal stress: implications for heat eradication methods in water distribution systems. Appl Environ Microbiol. 1986 Aug;52(2):396–399. [PMC free article] [PubMed]
  • Vickers RM, Stout JE, Tompkins LS, Troup NJ, Yu VL. Cefamandole-susceptible strains of Legionella pneumophila serogroup 1: implications for diagnosis and utility as an epidemiological marker. J Clin Microbiol. 1992 Mar;30(3):537–539. [PMC free article] [PubMed]
  • Vickers RM, Stout JE, Yu VL, Rihs JD. Manual of culture methodology for Legionella. Semin Respir Infect. 1987 Dec;2(4):274–279. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...