• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Oct 1993; 31(10): 2616–2620.
PMCID: PMC265946

Molecular analysis of multiple isolates of the major serotypes of group B streptococci.

Abstract

Serotyping of clinical isolates is a widely used technique for epidemiologic study of group B streptococcal infections. However, serotyping cannot definitively determine epidemiologically related or unrelated isolates. We investigated the use of restriction endonuclease analysis (REA) with both conventional agarose gel electrophoresis (AGE) and pulsed-field gel electrophoresis (PFGE) in 50 isolates of the major serotypes of group B streptococci. Single digestion with HindIII and HaeIII and double digestion with HindIII and then EcoRI were used for conventional AGE, and digestion with SmaI was used for PFGE. The molecular profile of one strain was compared with those of the strains within the same serotype as well as with the profiles from strains of different serotypes. Among 10 type Ia, Ia/alpha, Ia/alpha+beta, and Ia/R1 isolates and depending on the restriction enzyme used, we found between five and six REA patterns by conventional AGE and seven by PFGE; among 4 type Ib/alpha+beta isolates we found 2 to 4 REA patterns by conventional AGE and 4 by PFGE; among 21 type II, II/alpha, II/beta, II/alpha+beta, and II/R4 isolates, we found 11 REA patterns by both AGE and PFGE; and among 14 type III, III/R1, and III/R4 isolates, we found from 7 to 12 different REA patterns by AGE and 10 by PFGE. In total, among 13 serotypes and one nontypeable strain, we found 29 to 31 REA patterns by conventional AGE and 33 by PFGE. A particular REA pattern within a serotype was different from the patterns found in the other serotypes, suggesting that REA analysis by using conventional AGE or PFGE is a sensitive method for analyzing genetic relatedness and diversity in group B streptococci and has potential value in molecular epidemiologic studies.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Blumberg HM, Stephens DS, Licitra C, Pigott N, Facklam R, Swaminathan B, Wachsmuth IK. Molecular epidemiology of group B streptococcal infections: use of restriction endonuclease analysis of chromosomal DNA and DNA restriction fragment length polymorphisms of ribosomal RNA genes (ribotyping). J Infect Dis. 1992 Sep;166(3):574–579. [PubMed]
  • Caufield PW, Walker TM. Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. J Clin Microbiol. 1989 Feb;27(2):274–278. [PMC free article] [PubMed]
  • Denning DW, Baker CJ, Troup NJ, Tompkins LS. Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study. J Clin Microbiol. 1989 Jun;27(6):1352–1356. [PMC free article] [PubMed]
  • Ferrieri P. GBS infections in the newborn infant: diagnosis and treatment. Antibiot Chemother (1971) 1985;35:211–224. [PubMed]
  • Ferrieri P. Surface-localized protein antigens of group B streptococci. Rev Infect Dis. 1988 Jul-Aug;10 (Suppl 2):S363–S366. [PubMed]
  • Ferrieri P. Neonatal susceptibility and immunity to major bacterial pathogens. Rev Infect Dis. 1990 May-Jun;12 (Suppl 4):S394–S400. [PubMed]
  • Flores AE, Ferrieri P. Molecular species of R-protein antigens produced by clinical isolates of group B streptococci. J Clin Microbiol. 1989 May;27(5):1050–1054. [PMC free article] [PubMed]
  • Grothues D, Koopmann U, von der Hardt H, Tümmler B. Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains. J Clin Microbiol. 1988 Oct;26(10):1973–1977. [PMC free article] [PubMed]
  • Johnson DR, Ferrieri P. Group B streptococcal Ibc protein antigen: distribution of two determinants in wild-type strains of common serotypes. J Clin Microbiol. 1984 Apr;19(4):506–510. [PMC free article] [PubMed]
  • Klein NC, Schoch PE, Cunha BA. Nosocomial group B streptococcal infections. Infect Control Hosp Epidemiol. 1989 Oct;10(10):475–479. [PubMed]
  • Murray BE, Singh KV, Heath JD, Sharma BR, Weinstock GM. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol. 1990 Sep;28(9):2059–2063. [PMC free article] [PubMed]
  • Nagano Y, Nagano N, Takahashi S, Murono K, Fujita K, Taguchi F, Okuwaki Y. Restriction endonuclease digest patterns of chromosomal DNA from group B beta-haemolytic streptococci. J Med Microbiol. 1991 Nov;35(5):297–303. [PubMed]
  • Payne NR, Ferrieri P. The relation of the Ibc protein antigen to the opsonization differences between strains of type II group B streptococci. J Infect Dis. 1985 Apr;151(4):672–681. [PubMed]
  • Payne NR, Kim YK, Ferrieri P. Effect of differences in antibody and complement requirements on phagocytic uptake and intracellular killing of "c" protein-positive and -negative strains of type II group B streptococci. Infect Immun. 1987 May;55(5):1243–1251. [PMC free article] [PubMed]
  • Schwartz B, Schuchat A, Oxtoby MJ, Cochi SL, Hightower A, Broome CV. Invasive group B streptococcal disease in adults. A population-based study in metropolitan Atlanta. JAMA. 1991 Aug 28;266(8):1112–1114. [PubMed]
  • Single LA, Martin DR. Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electrophoresis. FEMS Microbiol Lett. 1992 Feb 1;70(1):85–89. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...