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J Clin Microbiol. 1993 Jun; 31(6): 1439–1443.
PMCID: PMC265558

Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction.


A polymerase chain reaction (PCR)-based test was developed for the detection of Salmonella typhi in the blood specimens from patients with typhoid fever. Two pairs of oligonucleotide primers were designed to amplify a 343-bp fragment of the flagellin gene of S. typhi. Amplified products were analyzed by agarose gel electrophoresis and Southern blot hybridization by using a 32P-labeled 40-base probe internal to the amplified DNA. The nested PCR with two pairs of primers could detect 10 organisms of S. typhi as determined by serial dilutions of DNA from S. typhi. The peripheral mononuclear cells from 11 of 12 patients with typhoid fever confirmed by blood culture were positive for DNA fragment of the flagellin gene of S. typhi, whereas 10 blood specimens of patients with other febrile diseases were negative. With the nested PCR, S. typhi DNAs were detected from blood specimens of four patients with suspected typhoid fever on the basis of clinical features but with negative cultures. We suggest that the PCR technique could be used as a novel diagnostic method of typhoid fever, particularly in culture-negative cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Edelman R, Levine MM. Summary of an international workshop on typhoid fever. Rev Infect Dis. 1986 May-Jun;8(3):329–349. [PubMed]
  • Frankel G, Newton SM, Schoolnik GK, Stocker BA. Intragenic recombination in a flagellin gene: characterization of the H1-j gene of Salmonella typhi. EMBO J. 1989 Oct;8(10):3149–3152. [PMC free article] [PubMed]
  • Frankel G, Newton SM, Schoolnik GK, Stocker BA. Unique sequences in region VI of the flagellin gene of Salmonella typhi. Mol Microbiol. 1989 Oct;3(10):1379–1383. [PubMed]
  • Gilman RH, Terminel M, Levine MM, Hernandez-Mendoza P, Hornick RB. Relative efficacy of blood, urine, rectal swab, bone-marrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet. 1975 May 31;1(7918):1211–1213. [PubMed]
  • Guerra-Caceres JG, Gotuzzo-Herencia E, Crosby-Dagnino E, Miro-Quesada M, Carrillo-Parodi C. Diagnostic value of bone marrow culture in typhoid fever. Trans R Soc Trop Med Hyg. 1979;73(6):680–683. [PubMed]
  • Hoffman SL, Edman DC, Punjabi NH, Lesmana M, Cholid A, Sundah S, Harahap J. Bone marrow aspirate culture superior to streptokinase clot culture and 8 ml 1:10 blood-to-broth ratio blood culture for diagnosis of typhoid fever. Am J Trop Med Hyg. 1986 Jul;35(4):836–839. [PubMed]
  • Levine MM, Grados O, Gilman RH, Woodward WE, Solis-Plaza R, Waldman W. Diagnostic value of the Widal test in areas endemic for typhoid fever. Am J Trop Med Hyg. 1978 Jul;27(4):795–800. [PubMed]
  • Rubin FA, Kopecko DJ, Noon KF, Baron LS. Development of a DNA probe to detect Salmonella typhi. J Clin Microbiol. 1985 Oct;22(4):600–605. [PMC free article] [PubMed]
  • Rubin FA, Kopecko DJ, Sack RB, Sudarmono P, Yi A, Maurta D, Meza R, Moechtar MA, Edman DC, Hoffman SL. Evaluation of a DNA probe for identifying Salmonella typhi in Peruvian and Indonesian bacterial isolates. J Infect Dis. 1988 May;157(5):1051–1053. [PubMed]
  • Rubin FA, McWhirter PD, Burr D, Punjabi NH, Lane E, Kumala S, Sudarmono P, Pulungsih SP, Lesmana M, Tjaniadi P, et al. Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood. J Clin Microbiol. 1990 Apr;28(4):825–827. [PMC free article] [PubMed]
  • Rubin FA, McWhirter PD, Punjabi NH, Lane E, Sudarmono P, Pulungsih SP, Lesmana M, Kumala S, Kopecko DJ, Hoffman SL. Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever. J Clin Microbiol. 1989 May;27(5):1112–1114. [PMC free article] [PubMed]
  • Wei LN, Joys TM. Covalent structure of three phase-1 flagellar filament proteins of Salmonella. J Mol Biol. 1985 Dec 20;186(4):791–803. [PubMed]
  • Wicks AC, Cruickshank JG, Musewe N. Observations on the diagnosis of typhoid fever in an endemic area. S Afr Med J. 1974 Jul 6;48(32):1368–1370. [PubMed]

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