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J Clin Microbiol. Aug 1992; 30(8): 2173–2176.
PMCID: PMC265465

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction.

Abstract

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

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  • Böddinghaus B, Rogall T, Flohr T, Blöcker H, Böttger EC. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol. 1990 Aug;28(8):1751–1759. [PMC free article] [PubMed]
  • Body BA, Warren NG, Spicer A, Henderson D, Chery M. Use of Gen-Probe and Bactec for rapid isolation and identification of mycobacteria. Correlation of probe results with growth index. Am J Clin Pathol. 1990 Mar;93(3):415–420. [PubMed]
  • Braun MM, Truman BI, Maguire B, DiFerdinando GT, Jr, Wormser G, Broaddus R, Morse DL. Increasing incidence of tuberculosis in a prison inmate population. Association with HIV infection. JAMA. 1989 Jan 20;261(3):393–397. [PubMed]
  • Brisson-Noël A, Gicquel B, Lecossier D, Lévy-Frébault V, Nassif X, Hance AJ. Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples. Lancet. 1989 Nov 4;2(8671):1069–1071. [PubMed]
  • Cesarone CF, Bolognesi C, Santi L. Improved microfluorometric DNA determination in biological material using 33258 Hoechst. Anal Biochem. 1979 Nov 15;100(1):188–197. [PubMed]
  • Cousins DV, Wilton SD, Francis BR. Use of DNA amplification for the rapid identification of Mycobacterium bovis. Vet Microbiol. 1991 Apr;27(2):187–195. [PubMed]
  • De Wit D, Steyn L, Shoemaker S, Sogin M. Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification. J Clin Microbiol. 1990 Nov;28(11):2437–2441. [PMC free article] [PubMed]
  • Eisenach KD, Cave MD, Bates JH, Crawford JT. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. [PubMed]
  • Kaneko K, Onodera O, Miyatake T, Tsuji S. Rapid diagnosis of tuberculous meningitis by polymerase chain reaction (PCR). Neurology. 1990 Oct;40(10):1617–1618. [PubMed]
  • Kirihara JM, Hillier SL, Coyle MB. Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system. J Clin Microbiol. 1985 Nov;22(5):841–845. [PMC free article] [PubMed]
  • Pao CC, Lin SS, Wu SY, Juang WM, Chang CH, Lin JY. The detection of mycobacterial DNA sequences in uncultured clinical specimens with cloned Mycobacterium tuberculosis DNA as probes. Tubercle. 1988 Mar;69(1):27–36. [PubMed]
  • Picken RN, Plotch SJ, Wang Z, Lin BC, Donegan JJ, Yang HL. DNA probes for mycobacteria. I. Isolation of DNA probes for the identification of Mycobacterium tuberculosis complex and for mycobacteria other than tuberculosis (MOTT). Mol Cell Probes. 1988 Jun;2(2):111–124. [PubMed]
  • Ralphs NT, Garrett S, Morse R, Cookson JB, Andrew PW, Boulnois GJ. A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens. J Appl Bacteriol. 1991 Mar;70(3):221–226. [PubMed]
  • Ratnam S, March SB. Effect of relative centrifugal force and centrifugation time on sedimentation of mycobacteria in clinical specimens. J Clin Microbiol. 1986 Mar;23(3):582–585. [PMC free article] [PubMed]
  • Sjöbring U, Mecklenburg M, Andersen AB, Miörner H. Polymerase chain reaction for detection of Mycobacterium tuberculosis. J Clin Microbiol. 1990 Oct;28(10):2200–2204. [PMC free article] [PubMed]

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