• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. May 1992; 30(5): 1323–1326.
PMCID: PMC265277

Defined medium simulating genital tract secretions for growth of vaginal microflora.


A chemically defined medium that simulates female genital tract secretions was developed for the growth of the vaginal microflora. Qualitative and quantitative studies of the growth of predominant components of the vaginal microflora indicated that all vaginal isolates tested were able to grow in this defined medium.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bartlett JG, Onderdonk AB, Drude E, Goldstein C, Anderka M, Alpert S, McCormack WM. Quantitative bacteriology of the vaginal flora. J Infect Dis. 1977 Aug;136(2):271–277. [PubMed]
  • Bonsall RW, Michael RP. Volatile constituents of primate vaginal secretions. J Reprod Fertil. 1971 Dec;27(3):478–479. [PubMed]
  • Cohen MS, Black JR, Proctor RA, Sparling PF. Host defences and the vaginal mucosa. A re-evaluation. Scand J Urol Nephrol Suppl. 1984;86:13–22. [PubMed]
  • Holmes KK, Chen KC, Lipinski CM, Eschenbach DA. Vaginal redox potential in bacterial vaginosis (nonspecific vaginitis). J Infect Dis. 1985 Aug;152(2):379–382. [PubMed]
  • Huggins GR, Preti G. Volatile constituents of human vaginal secretions. Am J Obstet Gynecol. 1976 Sep 1;126(1):129–136. [PubMed]
  • Huggins GR, Preti G. Vaginal odors and secretions. Clin Obstet Gynecol. 1981 Jun;24(2):355–377. [PubMed]
  • Michael RP, Bonsall RW, Kutner M. Volatile fatty acids, "copulins", in human vaginal secretions. Psychoneuroendocrinology. 1975;1(2):153–163. [PubMed]
  • Onderdonk AB, Polk BF, Moon NE, Goren B, Bartlett JG. Methods for quantitative vaginal flora studies. Am J Obstet Gynecol. 1977 Aug 1;128(7):777–781. [PubMed]
  • Onderdonk AB, Zamarchi GR, Rodriguez ML, Hirsch ML, Muñoz A, Kass EH. Quantitative assessment of vaginal microflora during use of tampons of various compositions. Appl Environ Microbiol. 1987 Dec;53(12):2774–2778. [PMC free article] [PubMed]
  • Paavonen J. Physiology and ecology of the vagina. Scand J Infect Dis Suppl. 1983;40:31–35. [PubMed]
  • Spiegel CA, Amsel R, Eschenbach D, Schoenknecht F, Holmes KK. Anaerobic bacteria in nonspecific vaginitis. N Engl J Med. 1980 Sep 11;303(11):601–607. [PubMed]
  • SUMAWONG V, GREGOIRE AT, JOHNSON WD, RAKOFF AE. Identification of carbohydrates in the vaginal fluid of normal females. Fertil Steril. 1962 May-Jun;13:270–280. [PubMed]
  • Wagner G, Levin RJ. Electrolytes in vaginal fluid during the menstrual cycle of coitally active and inactive women. J Reprod Fertil. 1980 Sep;60(1):17–27. [PubMed]
  • Wylie JG, Henderson A. Identity and glycogen-fermenting ability of lactobacilli isolated from the vagina of pregnant women. J Med Microbiol. 1969 Aug;2(3):363–366. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...