• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. May 1992; 30(5): 1179–1182.
PMCID: PMC265246

Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa, Zaire.

Abstract

The use of whole-blood spots on filter paper for the detection of antibody to human immunodeficiency virus type 1 (HIV-1) was evaluated during a 20-week period under a variety of storage environments simulating the harsh tropical field conditions in Kinshasa, Zaire. During the first 6 weeks of storage, all replicates of high- and low-titer HIV-1-positive reference samples remained positive by enzyme immunoassay and Western blotting (immunoblotting), and all replicates of HIV-1-negative samples remained negative under all storage conditions. However, hot and humid storage conditions for up to 20 weeks caused a progressive decline in enzyme immunoassay optical density ratio values, which was particularly noticeable in samples with a low HIV-1 antibody titer. Harsh tropical operational conditions did not cause any repeatedly false-positive results during the 20-week storage period. The use of gas-impermeable bags with desiccant for the storage of blood spots on filter paper improved the stability of HIV-1 antibody detection over time and is recommended for the storage of whole-blood spots on filter paper in harsh tropical field settings.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (762K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Beebe JL, Briggs LC. Evaluation of enzyme-linked immunoassay systems for detection of human immunodeficiency virus type 1 antibody from filter paper disks impregnated with whole blood. J Clin Microbiol. 1990 Apr;28(4):808–810. [PMC free article] [PubMed]
  • Dondero TJ, Jr, Pappaioanou M, Curran JW. Monitoring the levels and trends of HIV infection: the Public Health Service's HIV surveillance program. Public Health Rep. 1988 May-Jun;103(3):213–220. [PMC free article] [PubMed]
  • Farzadegan H, Quinn T, Polk BF. Detecting antibodies to human immunodeficiency virus in dried blood on filter papers. J Infect Dis. 1987 May;155(5):1073–1074. [PubMed]
  • Gwinn M, Pappaioanou M, George JR, Hannon WH, Wasser SC, Redus MA, Hoff R, Grady GF, Willoughby A, Novello AC, et al. Prevalence of HIV infection in childbearing women in the United States. Surveillance using newborn blood samples. JAMA. 1991 Apr 3;265(13):1704–1708. [PubMed]
  • Hannon WH, Lewis DS, Jones WK, Powell MK. A quality assurance program for human immunodeficiency virus seropositivity screening of dried-blood spot specimens. Infect Control Hosp Epidemiol. 1989 Jan;10(1):8–13. [PubMed]
  • Hoff R, Berardi VP, Weiblen BJ, Mahoney-Trout L, Mitchell ML, Grady GF. Seroprevalence of human immunodeficiency virus among childbearing women. Estimation by testing samples of blood from newborns. N Engl J Med. 1988 Mar 3;318(9):525–530. [PubMed]
  • Matuszak DL, Panny SR, Patel J, Israel E. HIV antibody seroprevalence among childbearing women surveyed in Maryland. Public Health Rep. 1990 Nov-Dec;105(6):562–566. [PMC free article] [PubMed]
  • Novick LF, Berns D, Stricof R, Stevens R, Pass K, Wethers J. HIV seroprevalence in newborns in New York State. JAMA. 1989 Mar 24;261(12):1745–1750. [PubMed]
  • Pappaioanou M, Dondero TJ, Jr, Petersen LR, Onorato IM, Sanchez CD, Curran JW. The family of HIV seroprevalence surveys: objectives, methods, and uses of sentinel surveillance for HIV in the United States. Public Health Rep. 1990 Mar-Apr;105(2):113–119. [PMC free article] [PubMed]
  • Peckham CS, Tedder RS, Briggs M, Ades AE, Hjelm M, Wilcox AH, Parra-Mejia N, O'Connor C. Prevalence of maternal HIV infection based on unlinked anonymous testing of newborn babies. Lancet. 1990 Mar 3;335(8688):516–519. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links