• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Apr 1992; 30(4): 905–910.
PMCID: PMC265183

Infection of peripheral blood mononuclear cells and cell lines by cell-free human T-cell lymphoma/leukemia virus type I.

Abstract

Previous studies of in vitro infection by human T-cell lymphoma/leukemia virus type I (HTLV-I) have required cocultivation of target cells with HTLV-I cell lines or vesicular stomatitis virus pseudotypes containing HTLV-I envelope proteins. We report here the development of a cell-free infection assay for HTLV-I. Target cells were incubated with purified, DNase-treated HTLV-I virions for 4 h at 37 degrees C. Target cell DNA was then analyzed for the presence of newly synthesized HTLV-I proviral DNA by the highly sensitive polymerase chain reaction. Using this assay system, we have been able to consistently detect in vitro infection of a variety of cellular targets by different HTLV-I isolates. Optimal infection required the presence of 10 micrograms of DEAE-dextran per ml. The assay was dose dependent with respect to virus input. In general, the amount of proviral DNA detected correlated with the level of HTLV-I receptors present on the surface of the target cells, as measured by fluorochrome-labelled HTLV-I binding. Finally, the specificity of the assay was confirmed by demonstrating that the cell line, L1q, a somatic cell hybrid containing human chromosome 17q, to which the gene for the HTLV-I receptor has been mapped, was susceptible to infection by HTLV-I, while the parental mouse cell line from which it was derived, LMTK-, which lacks human chromosome 17q, was not.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bhagavati S, Ehrlich G, Kula RW, Kwok S, Sninsky J, Udani V, Poiesz BJ. Detection of human T-cell lymphoma/leukemia virus type I DNA and antigen in spinal fluid and blood of patients with chronic progressive myelopathy. N Engl J Med. 1988 May 5;318(18):1141–1147. [PubMed]
  • Byrne BC, Li JJ, Sninsky J, Poiesz BJ. Detection of HIV-1 RNA sequences by in vitro DNA amplification. Nucleic Acids Res. 1988 May 11;16(9):4165–4165. [PMC free article] [PubMed]
  • Clapham P, Nagy K, Cheingsong-Popov R, Exley M, Weiss RA. Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line. Science. 1983 Dec 9;222(4628):1125–1127. [PubMed]
  • de Rossi A, Aldovini A, Franchini G, Mann D, Gallo RC, Wong-Staal F. Clonal selection of T lymphocytes infected by cell-free human T-cell leukemia/lymphoma virus type I: parameters of virus integration and expression. Virology. 1985 Jun;143(2):640–645. [PubMed]
  • Ehrlich GD, Davey FR, Kirshner JJ, Sninsky JJ, Kwok S, Slamon DJ, Kalish R, Poiesz BJ. A polyclonal CD4+ and CD8+ lymphocytosis in a patient doubly infected with HTLV-I and HIV-1: a clinical and molecular analysis. Am J Hematol. 1989 Mar;30(3):128–139. [PubMed]
  • Ehrlich GD, Glaser JB, LaVigne K, Quan D, Mildvan D, Sninsky JJ, Kwok S, Papsidero L, Poiesz BJ. Prevalence of human T-cell leukemia/lymphoma virus (HTLV) type II infection among high-risk individuals: type-specific identification of HTLVs by polymerase chain reaction. Blood. 1989 Oct;74(5):1658–1664. [PubMed]
  • Graziano SL, Lehr BM, Merl SA, Ehrlich GD, Moore JL, Hallinan EJ, Hubbell C, Davey FR, Vournakis J, Poiesz BJ. Quantitative assay of human T-cell leukemia/lymphoma virus transformation. Cancer Res. 1987 May 1;47(9):2468–2473. [PubMed]
  • Greenberg SM, Rosenthal DS, Greeley TA, Tantravahi R, Handin RI. Characterization of a new megakaryocytic cell line: the Dami cell. Blood. 1988 Dec;72(6):1968–1977. [PubMed]
  • Hansen JE, Nielsen C, Mathiesen LR, Nielsen JO. Involvement of lymphocyte function-associated antigen-1 (LFA-1) in HIV infection: inhibition by monoclonal antibody. Scand J Infect Dis. 1991;23(1):31–36. [PubMed]
  • Ho DD, Rota TR, Hirsch MS. Infection of human endothelial cells by human T-lymphotropic virus type I. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7588–7590. [PMC free article] [PubMed]
  • Huang CC, Hou Y, Woods LK, Moore GE, Minowada J. Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia. J Natl Cancer Inst. 1974 Sep;53(3):655–660. [PubMed]
  • Krichbaum-Stenger K, Poiesz BJ, Keller P, Ehrlich G, Gavalchin J, Davis BH, Moore JL. Specific adsorption of HTLV-I to various target human and animal cells. Blood. 1987 Nov;70(5):1303–1311. [PubMed]
  • Merl S, Kloster B, Moore J, Hubbell C, Tomar R, Davey F, Kalinowski D, Planas A, Ehrlich G, Clark D, et al. Efficient transformation of previously activated and dividing T lymphocytes by human T cell leukemia-lymphoma virus. Blood. 1984 Nov;64(5):967–974. [PubMed]
  • Miyoshi I, Kubonishi I, Yoshimoto S, Akagi T, Ohtsuki Y, Shiraishi Y, Nagata K, Hinuma Y. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature. 1981 Dec 24;294(5843):770–771. [PubMed]
  • Papsidero LD, Dittmer RP, Vaickus L, Poiesz BJ. Monoclonal antibodies and chemiluminescence immunoassay for detection of the surface protein of human T-cell lymphotropic virus. J Clin Microbiol. 1992 Feb;30(2):351–358. [PMC free article] [PubMed]
  • Papsidero L, Swartzwelder F, Sheu M, Montagna R, Ehrlich G, Bhagavati S, Dosik H, Sninsky J, Poiesz B. Immunodetection of human T-cell lymphotropic virus type I core protein in biological samples by using a monoclonal antibody immunoassay. J Clin Microbiol. 1990 May;28(5):949–955. [PMC free article] [PubMed]
  • Poiesz BJ, Ruscetti FW, Mier JW, Woods AM, Gallo RC. T-cell lines established from human T-lymphocytic neoplasias by direct response to T-cell growth factor. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6815–6819. [PMC free article] [PubMed]
  • Poiesz BJ, Ruscetti FW, Reitz MS, Kalyanaraman VS, Gallo RC. Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukaemia. Nature. 1981 Nov 19;294(5838):268–271. [PubMed]
  • Ratner L, Poiesz BJ. Leukemias associated with human T-cell lymphotropic virus type I in a non-endemic region. Medicine (Baltimore) 1988 Nov;67(6):401–422. [PubMed]
  • Saida T, Saida K, Funauchi M, Nishiguchi E, Nakajima M, Matsuda S, Ohta M, Ohta K, Nishitani H, Hatanaka M. HTLV-I myelitis: isolation of virus, genomic analysis, and infection in neural cell cultures. Ann N Y Acad Sci. 1988;540:636–638. [PubMed]
  • Sanford JA, Stubblefield E. General protocol for microcell-mediated chromosome transfer. Somat Cell Mol Genet. 1987 May;13(3):279–284. [PubMed]
  • Seto A, Isono T, Ogawa K. Infection of inbred rabbits with cell-free HTLV-I. Leuk Res. 1991;15(2-3):105–110. [PubMed]
  • Sommerfelt MA, Williams BP, Clapham PR, Solomon E, Goodfellow PN, Weiss RA. Human T cell leukemia viruses use a receptor determined by human chromosome 17. Science. 1988 Dec 16;242(4885):1557–1559. [PubMed]
  • Wells KH, Byrne BC, Poiesz BJ. Detection, prevention, and treatment of retroviral infections. Semin Oncol. 1990 Jun;17(3):295–320. [PubMed]
  • Yamamoto N, Matsumoto T, Koyanagi Y, Tanaka Y, Hinuma Y. Unique cell lines harbouring both Epstein-Barr virus and adult T-cell leukaemia virus, established from leukaemia patients. Nature. 1982 Sep 23;299(5881):367–369. [PubMed]
  • Yanagihara R, Nerurkar VR, Garruto RM, Miller MA, Leon-Monzon ME, Jenkins CL, Sanders RC, Liberski PP, Alpers MP, Gajdusek DC. Characterization of a variant of human T-lymphotropic virus type I isolated from a healthy member of a remote, recently contacted group in Papua New Guinea. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1446–1450. [PMC free article] [PubMed]
  • Zack JA, Arrigo SJ, Weitsman SR, Go AS, Haislip A, Chen IS. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure. Cell. 1990 Apr 20;61(2):213–222. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links