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Plant Cell. 2004; 16 (Suppl): S107–S118.
Published online Mar 9, 2004. doi:  10.1105/tpc.016873
PMCID: PMC2643384

Experimental analysis of the fertilization process.

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Selected References

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  • Antoine AF, Faure JE, Cordeiro S, Dumas C, Rougier M, Feijó JA. A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants. Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10643–10648. [PMC free article] [PubMed]
  • Antoine AF, Faure JE, Dumas C, Feijó JA. Differential contribution of cytoplasmic Ca2+ and Ca2+ influx to gamete fusion and egg activation in maize. Nat Cell Biol. 2001 Dec;3(12):1120–1123. [PubMed]
  • Carlson WR. Factors affecting preferential fertilization in maize. Genetics. 1969 Jul;62(3):543–554. [PMC free article] [PubMed]
  • Christensen Cory A, Gorsich Steven W, Brown Ryan H, Jones Linda G, Brown Jessica, Shaw Janet M, Drews Gary N. Mitochondrial GFA2 is required for synergid cell death in Arabidopsis. Plant Cell. 2002 Sep;14(9):2215–2232. [PMC free article] [PubMed]
  • Cordts S, Bantin J, Wittich PE, Kranz E, Lörz H, Dresselhaus T. ZmES genes encode peptides with structural homology to defensins and are specifically expressed in the female gametophyte of maize. Plant J. 2001 Jan;25(1):103–114. [PubMed]
  • Drews Gary N, Yadegari Ramin. Development and function of the angiosperm female gametophyte. Annu Rev Genet. 2002;36:99–124. [PubMed]
  • Edlund Anna F, Swanson Robert, Preuss Daphne. Pollen and stigma structure and function: the role of diversity in pollination. Plant Cell. 2004;16 (Suppl):S84–S97. [PMC free article] [PubMed]
  • Faure JE. Double fertilization in flowering plants: discovery, study methods and mechanisms. C R Acad Sci III. 2001 Jun;324(6):551–558. [PubMed]
  • Faure JE, Digonnet C, Dumas C. An in vitro system for adhesion and fusion of maize gametes. Science. 1994 Mar 18;263(5153):1598–1600. [PubMed]
  • Faure Jean-Emmanuel, Rotman Nicolas, Fortuné Philippe, Dumas Christian. Fertilization in Arabidopsis thaliana wild type: developmental stages and time course. Plant J. 2002 May;30(4):481–488. [PubMed]
  • Faure Jean-Emmanuel, Rusche Maxine Losoff, Thomas Annie, Keim Paul, Dumas Christian, Mogensen H Lloyd, Rougier Mireille, Chaboud Annie. Double fertilization in maize: the two male gametes from a pollen grain have the ability to fuse with egg cells. Plant J. 2003 Mar;33(6):1051–1062. [PubMed]
  • Friedman WE. Expression of the cell cycle in sperm of Arabidopsis: implications for understanding patterns of gametogenesis and fertilization in plants and other eukaryotes. Development. 1999 Feb;126(5):1065–1075. [PubMed]
  • Fu Y, Wu G, Yang Z. Rop GTPase-dependent dynamics of tip-localized F-actin controls tip growth in pollen tubes. J Cell Biol. 2001 Mar 5;152(5):1019–1032. [PMC free article] [PubMed]
  • Goodner B, Quatrano RS. Fucus Embryogenesis: A Model to Study the Establishment of Polarity. Plant Cell. 1993 Oct;5(10):1471–1481. [PMC free article] [PubMed]
  • Han YZ, Huang BQ, Zee SY, Yuan M. Symplastic communication between the central cell and the egg apparatus cells in the embryo sac of Torenia fournieri Lind. before and during fertilization. Planta. 2000 Jun;211(1):158–162. [PubMed]
  • Hepler PK, Vidali L, Cheung AY. Polarized cell growth in higher plants. Annu Rev Cell Dev Biol. 2001;17:159–187. [PubMed]
  • Higashiyama Tetsuya. The synergid cell: attractor and acceptor of the pollen tube for double fertilization. J Plant Res. 2002 Apr;115(1118):149–160. [PubMed]
  • Higashiyama T, Kuroiwa H, Kawano S, Kuroiwa T. Guidance in vitro of the pollen tube to the naked embryo sac of torenia fournieri . Plant Cell. 1998 Dec;10(12):2019–2032. [PMC free article] [PubMed]
  • Higashiyama T, Kuroiwa H, Kawano S, Kuroiwa T. Explosive discharge of pollen tube contents in Torenia fournieri. Plant Physiol. 2000 Jan;122(1):11–14. [PMC free article] [PubMed]
  • Higashiyama Tetsuya, Kuroiwa Haruko, Kuroiwa Tsuneyoshi. Pollen-tube guidance: beacons from the female gametophyte. Curr Opin Plant Biol. 2003 Feb;6(1):36–41. [PubMed]
  • Higashiyama T, Yabe S, Sasaki N, Nishimura Y, Miyagishima S, Kuroiwa H, Kuroiwa T. Pollen tube attraction by the synergid cell. Science. 2001 Aug 24;293(5534):1480–1483. [PubMed]
  • Huang BQ, Fu Y, Zee SY, Hepler PK. Three-dimensional organization and dynamic changes of the actin cytoskeleton in embryo sacs of Zea mays and Torenia fournieri. Protoplasma. 1999;209(1-2):105–119. [PubMed]
  • Huck Norbert, Moore James M, Federer Michael, Grossniklaus Ueli. The Arabidopsis mutant feronia disrupts the female gametophytic control of pollen tube reception. Development. 2003 May;130(10):2149–2159. [PubMed]
  • Hulskamp M, Schneitz K, Pruitt RE. Genetic Evidence for a Long-Range Activity That Directs Pollen Tube Guidance in Arabidopsis. Plant Cell. 1995 Jan;7(1):57–64. [PMC free article] [PubMed]
  • Johnson Mark A, Preuss Daphne. Plotting a course: multiple signals guide pollen tubes to their targets. Dev Cell. 2002 Mar;2(3):273–281. [PubMed]
  • Kao Teh-hui, Tsukamoto Tatsuya. The molecular and genetic bases of S-RNase-based self-incompatibility. Plant Cell. 2004;16 (Suppl):S72–S83. [PMC free article] [PubMed]
  • Kim Hyun Uk, Cotter Robyn, Johnson Sheila, Senda Mineo, Dodds Peter, Kulikauska Rima, Tang Weihua, Ezcura Ines, Herzmark Paul, McCormick Sheila. New pollen-specific receptor kinases identified in tomato, maize and Arabidopsis: the tomato kinases show overlapping but distinct localization patterns on pollen tubes. Plant Mol Biol. 2002 Sep;50(1):1–16. [PubMed]
  • Kranz E, Lorz H. In Vitro Fertilization with Isolated, Single Gametes Results in Zygotic Embryogenesis and Fertile Maize Plants. Plant Cell. 1993 Jul;5(7):739–746. [PMC free article] [PubMed]
  • Kranz E, Lörz H. In vitro fertilisation of maize by single egg and sperm cell protoplast fusion mediated by high calcium and high pH. Zygote. 1994 May;2(2):125–128. [PubMed]
  • Kranz E, von Wiegen P, Quader H, Lorz H. Endosperm development after fusion of isolated, single maize sperm and central cells in vitro . Plant Cell. 1998 Apr;10(4):511–524. [PMC free article] [PubMed]
  • Le Jie, El-Assal Salah El-Din, Basu Dipanwita, Saad Mohamed E, Szymanski Daniel B. Requirements for Arabidopsis ATARP2 and ATARP3 during epidermal development. Curr Biol. 2003 Aug 5;13(15):1341–1347. [PubMed]
  • Li H, Lin Y, Heath RM, Zhu MX, Yang Z. Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tip-localized calcium influx. Plant Cell. 1999 Sep;11(9):1731–1742. [PMC free article] [PubMed]
  • Li Shundai, Blanchoin Laurent, Yang Zhenbiao, Lord Elizabeth M. The putative Arabidopsis arp2/3 complex controls leaf cell morphogenesis. Plant Physiol. 2003 Aug;132(4):2034–2044. [PMC free article] [PubMed]
  • Li ST, Huang XY, Sun FZ. Flowering plant sperm contains a cytosolic soluble protein factor which can trigger calcium oscillations in mouse eggs. Biochem Biophys Res Commun. 2001 Sep 14;287(1):56–59. [PubMed]
  • Lord Elizabeth M, Russell Scott D. The mechanisms of pollination and fertilization in plants. Annu Rev Cell Dev Biol. 2002;18:81–105. [PubMed]
  • Lush WM. Whither chemotropism and pollen tube guidance? Trends Plant Sci. 1999 Oct;4(10):413–418. [PubMed]
  • Mascarenhas JP. Molecular Mechanisms of Pollen Tube Growth and Differentiation. Plant Cell. 1993 Oct;5(10):1303–1314. [PMC free article] [PubMed]
  • Mathur Jaideep, Mathur Neeta, Kernebeck Birgit, Hülskamp Martin. Mutations in actin-related proteins 2 and 3 affect cell shape development in Arabidopsis. Plant Cell. 2003 Jul;15(7):1632–1645. [PMC free article] [PubMed]
  • Mathur Jaideep, Mathur Neeta, Kirik Victor, Kernebeck Birgit, Srinivas Bhylahalli Purushottam, Hülskamp Martin. Arabidopsis CROOKED encodes for the smallest subunit of the ARP2/3 complex and controls cell shape by region specific fine F-actin formation. Development. 2003 Jul;130(14):3137–3146. [PubMed]
  • McCormick Sheila. Control of male gametophyte development. Plant Cell. 2004;16 (Suppl):S142–S153. [PMC free article] [PubMed]
  • Mogensen HL. Exclusion of male mitochondria and plastids during syngamy in barley as a basis for maternal inheritance. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2594–2597. [PMC free article] [PubMed]
  • Mogensen HL, Rusche ML. Occurrence of plastids in rye (Poaceae) sperm cells. Am J Bot. 2000 Aug;87(8):1189–1192. [PubMed]
  • Mól R, Idzikowska K, Dumas C, Matthys-Rochon E. Late steps of egg cell differentiation are accelerated by pollination in Zea mays L. Planta. 2000 Apr;210(5):749–757. [PubMed]
  • Molendijk AJ, Bischoff F, Rajendrakumar CS, Friml J, Braun M, Gilroy S, Palme K. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO J. 2001 Jun 1;20(11):2779–2788. [PMC free article] [PubMed]
  • Moutinho A, Hussey PJ, Trewavas AJ, Malhó R. cAMP acts as a second messenger in pollen tube growth and reorientation. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10481–10486. [PMC free article] [PubMed]
  • Muschietti J, Eyal Y, McCormick S. Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2. Plant Cell. 1998 Mar;10(3):319–330. [PMC free article] [PubMed]
  • Nagata N, Saito C, Sakai A, Kuroiwa H, Kuroiwa T. The selective increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls cytoplasmic inheritance. Planta. 1999 Jul;209(1):53–65. [PubMed]
  • O'Neill Sharman D. POLLINATION REGULATION OF FLOWER DEVELOPMENT. Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48(NaN):547–574. [PubMed]
  • Palanivelu Ravishankar, Brass Laura, Edlund Anna F, Preuss Daphne. Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell. 2003 Jul 11;114(1):47–59. [PubMed]
  • Palanivelu R, Preuss D. Pollen tube targeting and axon guidance: parallels in tip growth mechanisms. Trends Cell Biol. 2000 Dec;10(12):517–524. [PubMed]
  • Ray SM, Park SS, Ray A. Pollen tube guidance by the female gametophyte. Development. 1997 Jun;124(12):2489–2498. [PubMed]
  • Rotman Nicolas, Rozier Frédérique, Boavida Leonor, Dumas Christian, Berger Frédéric, Faure Jean-Emmanuel. Female control of male gamete delivery during fertilization in Arabidopsis thaliana. Curr Biol. 2003 Mar 4;13(5):432–436. [PubMed]
  • Rusche ML, Mogensen HL, Shi L, Keim P, Rougier M, Chaboud A, Dumas C. B chromosome behavior in maize pollen as determined by a molecular probe. Genetics. 1997 Dec;147(4):1915–1921. [PMC free article] [PubMed]
  • Russell SD. Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6129–6132. [PMC free article] [PubMed]
  • Saito C, Nagata N, Sakai A, Kuroiwa H, Kuroiwa T. Behavior of plastid nucleoids during male gametogenesis in Plumbago auriculata. Protoplasma. 2001;216(3-4):143–154. [PubMed]
  • Sanchez Ana Maria, Bosch Maurice, Bots Marc, Nieuwland Jeroen, Feron Richard, Mariani Celestina. Pistil factors controlling pollination. Plant Cell. 2004;16 (Suppl):S98–106. [PMC free article] [PubMed]
  • Shimizu KK, Okada K. Attractive and repulsive interactions between female and male gametophytes in Arabidopsis pollen tube guidance. Development. 2000 Oct;127(20):4511–4518. [PubMed]
  • Shivanna KR, Xu H, Taylor P, Knox RB. Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization. Plant Physiol. 1988 Jul;87(3):647–650. [PMC free article] [PubMed]
  • Singh Manjit, Bhalla Prem L, Xu Huiling, Singh Mohan B. Isolation and characterization of a flowering plant male gametic cell-specific promoter. FEBS Lett. 2003 May 8;542(1-3):47–52. [PubMed]
  • Stricker SA. Comparative biology of calcium signaling during fertilization and egg activation in animals. Dev Biol. 1999 Jul 15;211(2):157–176. [PubMed]
  • Weterings Koen, Pezzotti Mario, Cornelissen Marc, Mariani Celestina. Dynamic 1-aminocyclopropane-1-carboxylate-synthase and -oxidase transcript accumulation patterns during pollen tube growth in tobacco styles. Plant Physiol. 2002 Nov;130(3):1190–1200. [PMC free article] [PubMed]
  • Wilhelmi LK, Preuss D. Self-sterility in Arabidopsis due to defective pollen tube guidance. Science. 1996 Nov 29;274(5292):1535–1537. [PubMed]
  • Yadegari Ramin, Drews Gary N. Female gametophyte development. Plant Cell. 2004;16 (Suppl):S133–S141. [PMC free article] [PubMed]
  • Yang Zhenbiao. Small GTPases: versatile signaling switches in plants. Plant Cell. 2002;14 (Suppl):S375–S388. [PMC free article] [PubMed]
  • Ye Xiu-Lin, Yeung Edward C, Zee Sze-Yong. Sperm movement during double fertilization of a flowering plant, Phaius tankervilliae. Planta. 2002 May;215(1):60–66. [PubMed]
  • Yu HS, Russell SD. Three-dimensional ultrastructure of generative cell mitosis in the pollen tube of Nicotiana tabacum. Eur J Cell Biol. 1993 Aug;61(2):338–348. [PubMed]
  • Zhang XS, O'Neill SD. Ovary and Gametophyte Development Are Coordinately Regulated by Auxin and Ethylene following Pollination. Plant Cell. 1993 Apr;5(4):403–418. [PMC free article] [PubMed]
  • Zheng ZL, Yang Z. The Rrop GTPase switch turns on polar growth in pollen. Trends Plant Sci. 2000 Jul;5(7):298–303. [PubMed]

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