• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of eidLink to Publisher's site
Emerg Infect Dis. 1998 Oct-Dec; 4(4): 551–560.
PMCID: PMC2640238

Cell-to-cell signaling and Pseudomonas aeruginosa infections.

Abstract

Pseudomonas aeruginosa is a bacterium responsible for severe nosocomial infections, life-threatening infections in immunocompromised persons, and chronic infections in cystic fibrosis patients. The bacterium's virulence depends on a large number of cell-associated and extracellular factors. Cell-to-cell signaling systems control the expression and allow a coordinated, cell-density-dependent production of many extracellular virulence factors. We discuss the possible role of cell-to-cell signaling in the pathogenesis of P. aeruginosa infections and present a rationale for targeting cell-to-cell signaling systems in the development of new therapeutic approaches.

Full Text

The Full Text of this article is available as a PDF (174K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gordon SM, Serkey JM, Keys TF, Ryan T, Fatica CA, Schmitt SK, Borsh JA, Cosgrove DM, 3rd, Yared JP. Secular trends in nosocomial bloodstream infections in a 55-bed cardiothoracic intensive care unit. Ann Thorac Surg. 1998 Jan;65(1):95–100. [PubMed]
  • Fergie JE, Shema SJ, Lott L, Crawford R, Patrick CC. Pseudomonas aeruginosa bacteremia in immunocompromised children: analysis of factors associated with a poor outcome. Clin Infect Dis. 1994 Mar;18(3):390–394. [PubMed]
  • Bergen GA, Shelhamer JH. Pulmonary infiltrates in the cancer patient. New approaches to an old problem. Infect Dis Clin North Am. 1996 Jun;10(2):297–325. [PubMed]
  • Dunn M, Wunderink RG. Ventilator-associated pneumonia caused by Pseudomonas infection. Clin Chest Med. 1995 Mar;16(1):95–109. [PubMed]
  • de Bentzmann S, Roger P, Dupuit F, Bajolet-Laudinat O, Fuchey C, Plotkowski MC, Puchelle E. Asialo GM1 is a receptor for Pseudomonas aeruginosa adherence to regenerating respiratory epithelial cells. Infect Immun. 1996 May;64(5):1582–1588. [PMC free article] [PubMed]
  • Woods DE, Iglewski BH. Toxins of Pseudomonas aeruginosa: new perspectives. Rev Infect Dis. 1983 Sep-Oct;5 (Suppl 4):S715–S722. [PubMed]
  • Read RC, Roberts P, Munro N, Rutman A, Hastie A, Shryock T, Hall R, McDonald-Gibson W, Lund V, Taylor G, et al. Effect of Pseudomonas aeruginosa rhamnolipids on mucociliary transport and ciliary beating. J Appl Physiol (1985) 1992 Jun;72(6):2271–2277. [PubMed]
  • Kernacki KA, Hobden JA, Hazlett LD, Fridman R, Berk RS. In vivo bacterial protease production during Pseudomonas aeruginosa corneal infection. Invest Ophthalmol Vis Sci. 1995 Jun;36(7):1371–1378. [PubMed]
  • Heck LW, Morihara K, McRae WB, Miller EJ. Specific cleavage of human type III and IV collagens by Pseudomonas aeruginosa elastase. Infect Immun. 1986 Jan;51(1):115–118. [PMC free article] [PubMed]
  • Heck LW, Alarcon PG, Kulhavy RM, Morihara K, Russell MW, Mestecky JF. Degradation of IgA proteins by Pseudomonas aeruginosa elastase. J Immunol. 1990 Mar 15;144(6):2253–2257. [PubMed]
  • Jacquot J, Tournier JM, Puchelle E. In vitro evidence that human airway lysozyme is cleaved and inactivated by Pseudomonas aeruginosa elastase and not by human leukocyte elastase. Infect Immun. 1985 Feb;47(2):555–560. [PMC free article] [PubMed]
  • Johnson DA, Carter-Hamm B, Dralle WM. Inactivation of human bronchial mucosal proteinase inhibitor by Pseudomonas aeruginosa elastase. Am Rev Respir Dis. 1982 Dec;126(6):1070–1073. [PubMed]
  • Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldberg JB, Prince A. Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infect Immun. 1996 Jan;64(1):37–43. [PMC free article] [PubMed]
  • Fuqua C, Winans SC, Greenberg EP. Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu Rev Microbiol. 1996;50:727–751. [PubMed]
  • Gray KM. Intercellular communication and group behavior in bacteria. Trends Microbiol. 1997 May;5(5):184–188. [PubMed]
  • Clough SJ, Lee KE, Schell MA, Denny TP. A two-component system in Ralstonia (Pseudomonas) solanacearum modulates production of PhcA-regulated virulence factors in response to 3-hydroxypalmitic acid methyl ester. J Bacteriol. 1997 Jun;179(11):3639–3648. [PMC free article] [PubMed]
  • Flavier AB, Clough SJ, Schell MA, Denny TP. Identification of 3-hydroxypalmitic acid methyl ester as a novel autoregulator controlling virulence in Ralstonia solanacearum. Mol Microbiol. 1997 Oct;26(2):251–259. [PubMed]
  • Stevens AM, Dolan KM, Greenberg EP. Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12619–12623. [PMC free article] [PubMed]
  • Stevens AM, Greenberg EP. Quorum sensing in Vibrio fischeri: essential elements for activation of the luminescence genes. J Bacteriol. 1997 Jan;179(2):557–562. [PMC free article] [PubMed]
  • Passador L, Cook JM, Gambello MJ, Rust L, Iglewski BH. Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science. 1993 May 21;260(5111):1127–1130. [PubMed]
  • Pearson JP, Gray KM, Passador L, Tucker KD, Eberhard A, Iglewski BH, Greenberg EP. Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):197–201. [PMC free article] [PubMed]
  • Gambello MJ, Kaye S, Iglewski BH. LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression. Infect Immun. 1993 Apr;61(4):1180–1184. [PMC free article] [PubMed]
  • Seed PC, Passador L, Iglewski BH. Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: an autoinduction regulatory hierarchy. J Bacteriol. 1995 Feb;177(3):654–659. [PMC free article] [PubMed]
  • Chapon-Hervé V, Akrim M, Latifi A, Williams P, Lazdunski A, Bally M. Regulation of the xcp secretion pathway by multiple quorum-sensing modulons in Pseudomonas aeruginosa. Mol Microbiol. 1997 Jun;24(6):1169–1178. [PubMed]
  • Telford G, Wheeler D, Williams P, Tomkins PT, Appleby P, Sewell H, Stewart GS, Bycroft BW, Pritchard DI. The Pseudomonas aeruginosa quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone has immunomodulatory activity. Infect Immun. 1998 Jan;66(1):36–42. [PMC free article] [PubMed]
  • Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, Haas D. The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. Mol Microbiol. 1997 Apr;24(2):309–319. [PubMed]
  • Ochsner UA, Koch AK, Fiechter A, Reiser J. Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. J Bacteriol. 1994 Apr;176(7):2044–2054. [PMC free article] [PubMed]
  • Latifi A, Winson MK, Foglino M, Bycroft BW, Stewart GS, Lazdunski A, Williams P. Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1. Mol Microbiol. 1995 Jul;17(2):333–343. [PubMed]
  • Winson MK, Camara M, Latifi A, Foglino M, Chhabra SR, Daykin M, Bally M, Chapon V, Salmond GP, Bycroft BW, et al. Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9427–9431. [PMC free article] [PubMed]
  • Ochsner UA, Reiser J. Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6424–6428. [PMC free article] [PubMed]
  • Pearson JP, Pesci EC, Iglewski BH. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J Bacteriol. 1997 Sep;179(18):5756–5767. [PMC free article] [PubMed]
  • Latifi A, Foglino M, Tanaka K, Williams P, Lazdunski A. A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary-phase sigma factor RpoS. Mol Microbiol. 1996 Sep;21(6):1137–1146. [PubMed]
  • Loewen PC, Hengge-Aronis R. The role of the sigma factor sigma S (KatF) in bacterial global regulation. Annu Rev Microbiol. 1994;48:53–80. [PubMed]
  • Milton DL, Hardman A, Camara M, Chhabra SR, Bycroft BW, Stewart GS, Williams P. Quorum sensing in Vibrio anguillarum: characterization of the vanI/vanR locus and identification of the autoinducer N-(3-oxodecanoyl)-L-homoserine lactone. J Bacteriol. 1997 May;179(9):3004–3012. [PMC free article] [PubMed]
  • Passador L, Tucker KD, Guertin KR, Journet MP, Kende AS, Iglewski BH. Functional analysis of the Pseudomonas aeruginosa autoinducer PAI. J Bacteriol. 1996 Oct;178(20):5995–6000. [PMC free article] [PubMed]
  • Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Jr, Greenberg EP. Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9505–9509. [PMC free article] [PubMed]
  • Moré MI, Finger LD, Stryker JL, Fuqua C, Eberhard A, Winans SC. Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates. Science. 1996 Jun 14;272(5268):1655–1658. [PubMed]
  • Hanzelka BL, Stevens AM, Parsek MR, Crone TJ, Greenberg EP. Mutational analysis of the Vibrio fischeri LuxI polypeptide: critical regions of an autoinducer synthase. J Bacteriol. 1997 Aug;179(15):4882–4887. [PMC free article] [PubMed]
  • Parsek MR, Schaefer AL, Greenberg EP. Analysis of random and site-directed mutations in rhII, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase. Mol Microbiol. 1997 Oct;26(2):301–310. [PubMed]

Articles from Emerging Infectious Diseases are provided here courtesy of Centers for Disease Control and Prevention

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...