• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. Aug 1994; 32(8): 2007–2008.
PMCID: PMC263920

Identification and elimination of DNA sequences in Taq DNA polymerase.

Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (268K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Böttger EC. Frequent contamination of Taq polymerase with DNA. Clin Chem. 1990 Jun;36(6):1258–1259. [PubMed]
  • Hughes MS, Skuce RA, Beck LA, Neill SD. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J Clin Microbiol. 1993 Dec;31(12):3216–3222. [PMC free article] [PubMed]
  • Meier A, Persing DH, Finken M, Böttger EC. Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens. J Clin Microbiol. 1993 Mar;31(3):646–652. [PMC free article] [PubMed]
  • Rand KH, Houck H. Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes. 1990 Dec;4(6):445–450. [PubMed]
  • Schmidt TM, Pace B, Pace NR. Detection of DNA contamination in Taq polymerase. Biotechniques. 1991 Aug;11(2):176–177. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...