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Infect Immun. Sep 1984; 45(3): 697–700.
PMCID: PMC263352

Common and isolate-restricted antigens of Anaplasma marginale detected with monoclonal antibodies.

Abstract

Anaplasma marginale-infected erythrocytes were examined for the presence of maturation, isolate-restricted, and isolate-common antigens by indirect immunofluorescence with monoclonal antibodies. A panel of 18 monoclonal antibodies was used; none of the antibodies reacted with Anaplasma ovis, Babesia bigemina, babesia bovis, Trypanosoma brucei, Trypanosoma congolense, or uninfected bovine erythrocytes. Antigens common to all six A. marginale isolates were detected by nine antibodies. Single isolates from Florida, Southern Idaho, Northern Texas, and Virginia and two isolates from Washington state had four patterns of reactivity with a second panel of nine antibodies. Antigenically distinct stages were not detected, as sequential smears taken daily during acute infection had the same pattern of reactivity. The results demonstrate antigenic heterogeneity among isolates of A. marginale and the presence of common antigens. This information allows grouping of different isolates and, more importantly, provides a method for the identification and isolation of common antigens for diagnostic tests.

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Selected References

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  • Barbet AF, Anderson LW, Palmer GH, McGuire TC. Comparison of proteins synthesized by two different isolates of Anaplasma marginale. Infect Immun. 1983 Jun;40(3):1068–1074. [PMC free article] [PubMed]
  • Barbet AF, Davis WC, McGuire TC. Cross-neutralization of two different trypanosome populations derived from a single organism. Nature. 1982 Dec 2;300(5891):453–456. [PubMed]
  • Carson CA, Adams LG, Todorovic RA. An antigenic and serologic comparison of two virulent strains and an attenuated strain of Anaplasma marginale. Am J Vet Res. 1970 Jun;31(6):1071–1078. [PubMed]
  • Davis WC, Talmadge JE, Parish SM, Johnson MI, Vibber SD. Synthesis of DNA and protein by Anaplasma marginale in bovine erythrocytes during short-term culture. Infect Immun. 1978 Nov;22(2):597–602. [PMC free article] [PubMed]
  • Diffley P, Honigberg BM. Fluorescent antibody analysis of host plasma components on bloodstream forms or African pathogenic trypanosomes. I. Host specificity and time of accretion in Trypanosoma congolense. J Parasitol. 1977 Aug;63(4):599–606. [PubMed]
  • FAHEY JL, MCKELVEY EM. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed]
  • Kocan KM, Venable JH, Brock WE. Ultrastructure of anaplasmal inclusions (Pawhuska isolate) and their appendages in intact and hemolyzed erythrocytes and in complement-fixation antigen. Am J Vet Res. 1978 Jul;39(7):1123–1130. [PubMed]
  • KREIER JP, RISTIC M. Anaplasmosis. X. Morphologic characteristics of the parasites present in the blood of calves infected with the Oregon strain of Anaplasma marginale. Am J Vet Res. 1963 Jul;24:676–687. [PubMed]
  • KREIER JP, RISTIC M. Anaplasmosis. XI. Immunoserologic characteristics of the parasites present in the blood of calves infected with the Oregon strain of Anaplasma marginale. Am J Vet Res. 1963 Jul;24:688–696. [PubMed]
  • Kuttler KL, Johnson LW. Immunization of cattle with a Babesia bigemina antigen in Freund's complete adjuvant. Am J Vet Res. 1980 Apr;41(4):536–538. [PubMed]
  • Levy MG, Ristic M. Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture. Science. 1980 Mar 14;207(4436):1218–1220. [PubMed]
  • Love JN. Cryogenic preservation of Anaplasma marginale with Dimethyl sulfoxide. Am J Vet Res. 1972 Dec;33(12):2557–2560. [PubMed]
  • Magonigle RA, Eckblad WP, Lincoln SD, Frank FW. Anaplasma ovis in Idaho sheep. Am J Vet Res. 1981 Feb;42(2):199–201. [PubMed]
  • Ristic M, Carson CA. Methods of immunoprophylaxis against bovine anaplasmosis with emphasis on use of the attenuated Anaplasma marginale vaccine. Adv Exp Med Biol. 1977;93:151–188. [PubMed]
  • ROGERS TE, HIDALGO RJ, DIMOPOULLOS GT. IMMUNOLOGY AND SEROLOGY OF ANAPLASMA MARGINALE. I. FRACTIONATION OF THE COMPLEMENT-FIXING ANTIGEN. J Bacteriol. 1964 Jul;88:81–86. [PMC free article] [PubMed]

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