Logo of eidLink to Publisher's site
Emerg Infect Dis. 2000 Sep-Oct; 6(5): 477–480.
PMCID: PMC2627953

Naturally occurring Ehrlichia chaffeensis infection in coyotes from Oklahoma.


A nested polymerase chain reaction assay was used to determine the presence of Ehrlichia chaffeensis, E. canis, and E. ewingii DNA in blood samples of free-ranging coyotes from central and northcentral Oklahoma. Of the 21 coyotes examined, 15 (71%) were positive for E. chaffeensis DNA; none was positive for E. canis or E. ewingii. Results suggest that E. chaffeensis infections are common in free-ranging coyotes in Oklahoma and that these wild canids could play a role in the epidemiology of human monocytotropic ehrlichiosis.

Full Text

The Full Text of this article is available as a PDF (71K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Eng TR, Harkess JR, Fishbein DB, Dawson JE, Greene CN, Redus MA, Satalowich FT. Epidemiologic, clinical, and laboratory findings of human ehrlichiosis in the United States, 1988. JAMA. 1990 Nov 7;264(17):2251–2258. [PubMed]
  • Dawson JE, Childs JE, Biggie KL, Moore C, Stallknecht D, Shaddock J, Bouseman J, Hofmeister E, Olson JG. White-tailed deer as a potential reservoir of Ehrlichia spp. J Wildl Dis. 1994 Apr;30(2):162–168. [PubMed]
  • Ewing SA, Dawson JE, Kocan AA, Barker RW, Warner CK, Panciera RJ, Fox JC, Kocan KM, Blouin EF. Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae). J Med Entomol. 1995 May;32(3):368–374. [PubMed]
  • Lockhart JM, Davidson WR, Stallknecht DE, Dawson JE, Howerth EW. Isolation of Ehrlichia chaffeensis from wild white-tailed deer (Odocoileus virginianus) confirms their role as natural reservoir hosts. J Clin Microbiol. 1997 Jul;35(7):1681–1686. [PMC free article] [PubMed]
  • Lockhart JM, Davidson WR, Stallknecht DE, Dawson JE, Little SE. Natural history of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) in the piedmont physiographic province of Georgia. J Parasitol. 1997 Oct;83(5):887–894. [PubMed]
  • Lockhart JM, Davidson WR, Dawson JE, Stallknecht DE. Temporal association of Amblyomma americanum with the presence of Ehrlichia chaffeensis reactive antibodies in white-tailed deer. J Wildl Dis. 1995 Apr;31(2):119–124. [PubMed]
  • Magnarelli LA, Anderson JF, Stafford KC, 3rd, Dumler JS. Antibodies to multiple tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in white-footed mice. J Wildl Dis. 1997 Jul;33(3):466–473. [PubMed]
  • Davidson WR, Lockhart JM, Stallknecht DE, Howerth EW. Susceptibility of red and gray foxes to infection by Ehrlichia chaffeensis. J Wildl Dis. 1999 Oct;35(4):696–702. [PubMed]
  • Telford SR, 3rd, Dawson JE. Persistent infection of C3H/HeJ mice by Ehrlichia chaffeensis. Vet Microbiol. 1996 Sep;52(1-2):103–112. [PubMed]
  • Lockhart JM, Davidson WR, Stallknecht DE, Dawson JE. Lack of seroreactivity to Ehrlichia chaffeensis among rodent populations. J Wildl Dis. 1998 Apr;34(2):392–396. [PubMed]
  • Dawson JE, Ewing SA. Susceptibility of dogs to infection with Ehrlichia chaffeensis, causative agent of human ehrlichiosis. Am J Vet Res. 1992 Aug;53(8):1322–1327. [PubMed]
  • Dawson JE, Biggie KL, Warner CK, Cookson K, Jenkins S, Levine JF, Olson JG. Polymerase chain reaction evidence of Ehrlichia chaffeensis, an etiologic agent of human ehrlichiosis, in dogs from southeast Virginia. Am J Vet Res. 1996 Aug;57(8):1175–1179. [PubMed]
  • Murphy GL, Ewing SA, Whitworth LC, Fox JC, Kocan AA. A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet Parasitol. 1998 Nov 27;79(4):325–339. [PubMed]
  • Dawson JE, Warner CK, Baker V, Ewing SA, Stallknecht DE, Davidson WR, Kocan AA, Lockhart JM, Olson JG. Ehrlichia-like 16S rDNA sequence from wild white-tailed deer (Odocoileus virginianus). J Parasitol. 1996 Feb;82(1):52–58. [PubMed]
  • Anderson BE, Sumner JW, Dawson JE, Tzianabos T, Greene CR, Olson JG, Fishbein DB, Olsen-Rasmussen M, Holloway BP, George EH, et al. Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):775–780. [PMC free article] [PubMed]
  • Dawson JE, Stallknecht DE, Howerth EW, Warner C, Biggie K, Davidson WR, Lockhart JM, Nettles VF, Olson JG, Childs JE. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. J Clin Microbiol. 1994 Nov;32(11):2725–2728. [PMC free article] [PubMed]
  • Wilton SD, Lim L, Dye D, Laing N. Bandstab: a PCR-based alternative to cloning PCR products. Biotechniques. 1997 Apr;22(4):642–645. [PubMed]
  • Kocan AA, Breshears M, Cummings C, Panciera RJ, Ewing SA, Barker RW. Naturally occurring hepatozoonosis in coyotes from Oklahoma. J Wildl Dis. 1999 Jan;35(1):86–89. [PubMed]
  • Bloemer SR, Zimmerman RH. Ixodid ticks on the coyote and gray fox at land between the lakes, Kentucky-Tennessee, and implications for tick dispersal. J Med Entomol. 1988 Jan;25(1):5–8. [PubMed]
  • Buller RS, Arens M, Hmiel SP, Paddock CD, Sumner JW, Rikhisa Y, Unver A, Gaudreault-Keener M, Manian FA, Liddell AM, et al. Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N Engl J Med. 1999 Jul 15;341(3):148–155. [PubMed]
  • Anziani OS, Ewing SA, Barker RW. Experimental transmission of a granulocytic form of the tribe Ehrlichieae by Dermacentor variabilis and Amblyomma americanum to dogs. Am J Vet Res. 1990 Jun;51(6):929–931. [PubMed]

Articles from Emerging Infectious Diseases are provided here courtesy of Centers for Disease Control and Prevention


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...