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Infect Immun. Apr 1986; 52(1): 45–49.
PMCID: PMC262195

Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.

Abstract

A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V. cholerae non-O1 (strain A-5) by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatographies on SP-Sephadex C-50 and DEAE-Sephadex A-25, and high-pressure liquid chromatography on a Lichrosorb RP-8 column. About 1.4 X 10(5)-fold purification was achieved, with a recovery of about 12%. Although the crude preparation was heat labile, the purified toxin was heat stable. The minimum effective dose of purified toxin was about 5 ng in the suckling mouse assay. The amino acid composition of the purified toxin was determined to be Asp(3), Glu(1), Ala(1), half-Cys(6), Ile(2), Leu(1), Phe(1), and Pro(1). These data show the production of a new type of heat-stable enterotoxin (NAG-ST) by V. cholerae non-O1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aimoto S, Takao T, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T. Amino-acid sequence of a heat-stable enterotoxin produced by human enterotoxigenic Escherichia coli. Eur J Biochem. 1982 Dec 15;129(2):257–263. [PubMed]
  • Aldová E, Láznicková K, Stepánková E, Lietava J. Isolation of nonagglutinable vibrios from an enteritis outbreak in Czechoslovakia. J Infect Dis. 1968 Feb;118(1):25–31. [PubMed]
  • Blake PA, Weaver RE, Hollis DG. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol. 1980;34:341–367. [PubMed]
  • Craig JP, Yamamoto K, Takeda Y, Miwatani T. Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment. Infect Immun. 1981 Oct;34(1):90–97. [PMC free article] [PubMed]
  • Giannella RA. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. [PMC free article] [PubMed]
  • Johnson WM, Lior H. Cytotoxicity and suckling mouse reactivity of Aeromonas hydrophila isolated from human sources. Can J Microbiol. 1981 Oct;27(10):1019–1027. [PubMed]
  • Madden JM, Nematollahi WP, Hill WE, McCardell BA, Twedt RM. Virulence of three clinical isolates of Vibrio cholerae non-O-1 serogroup in experimental enteric infections in rabbits. Infect Immun. 1981 Aug;33(2):616–619. [PMC free article] [PubMed]
  • MCINTYRE OR, FEELEY JC, GREENOUGH WB, 3rd, BENENSON AS, HASSAN SI, SAAD A. DIARRHEA CAUSED BY NON-CHOLERA VIBRIOS. Am J Trop Med Hyg. 1965 May;14:412–418. [PubMed]
  • Nishibuchi M, Seidler RJ. Medium-dependent production of extracellular enterotoxins by non-O-1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis. Appl Environ Microbiol. 1983 Jan;45(1):228–231. [PMC free article] [PubMed]
  • Nishibuchi M, Seidler RJ, Rollins DM, Joseph SW. Vibrio factors cause rapid fluid accumulation in suckling mice. Infect Immun. 1983 Jun;40(3):1083–1091. [PMC free article] [PubMed]
  • Pai CH, Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. [PMC free article] [PubMed]
  • Sack RB. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. [PubMed]
  • Takao T, Hitouji T, Aimoto S, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T. Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D. FEBS Lett. 1983 Feb 7;152(1):1–5. [PubMed]
  • Takao T, Tominaga N, Shimonishi Y, Hara S, Inoue T, Miyama A. Primary structure of heat-stable enterotoxin produced by Yersinia enterocolitica. Biochem Biophys Res Commun. 1984 Dec 28;125(3):845–851. [PubMed]
  • Takeda Y, Takeda T, Yano T, Yamamoto K, Miwatani T. Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli. Infect Immun. 1979 Sep;25(3):978–985. [PMC free article] [PubMed]
  • Yamamoto K, Takeda Y, Miwatani T, Craig JP. Evidence that a non-O1 Vibrio cholerae produces enterotoxin that is similar but not identical to cholera enterotoxin. Infect Immun. 1983 Sep;41(3):896–901. [PMC free article] [PubMed]

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