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Infect Immun. Apr 1985; 48(1): 248–251.
PMCID: PMC261943

Haemophilus influenzae can use human transferrin as a sole source for required iron.


Haemophilus influenzae grown on enriched medium containing protoporphyrin IX rather than hemin was iron starved by the addition of the chelator ethylenediamine di-o-hydroxyphenylacetic acid. Iron starvation could be overcome in each of 33 H. influenzae type b isolates by 30% Fe-saturated human transferrin but not by human lactoferrin. Among nontypeable H. influenzae, 28 of 35 isolates, including 2 of 3 systemic isolates, were able to utilize Fe-transferrin. None of 18 H. parainfluenzae isolates was able to use Fe-transferrin. Iron starvation of H. influenzae type b resulted in increased amounts of three membrane proteins of 94,000 to 98,000 daltons.

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Selected References

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  • Brock JH, Pickering MG, McDowall MC, Deacon AG. Role of antibody and enterobactin in controlling growth of Escherichia coli in human milk and acquisition of lactoferrin- and transferrin-bound iron by Escherichia coli. Infect Immun. 1983 May;40(2):453–459. [PMC free article] [PubMed]
  • Eaton JW, Brandt P, Mahoney JR, Lee JT., Jr Haptoglobin: a natural bacteriostat. Science. 1982 Feb 5;215(4533):691–693. [PubMed]
  • Finch CA, Huebers H. Perspectives in iron metabolism. N Engl J Med. 1982 Jun 24;306(25):1520–1528. [PubMed]
  • Grahm G, Bates GW. Approaches to the standardization of serum unsaturated iron-binding capacity. J Lab Clin Med. 1976 Sep;88(3):477–486. [PubMed]
  • Helms SD, Oliver JD, Travis JC. Role of heme compounds and haptoglobin in Vibrio vulnificus pathogenicity. Infect Immun. 1984 Aug;45(2):345–349. [PMC free article] [PubMed]
  • Kochan I, Kvach JT, Wiles TI. Virulence-associated acquisition of iron in mammalian serum by Escherichia coli. J Infect Dis. 1977 Apr;135(4):623–632. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. [PubMed]
  • Masson PL, Heremans JF, Schonne E. Lactoferrin, an iron-binding protein in neutrophilic leukocytes. J Exp Med. 1969 Sep 1;130(3):643–658. [PMC free article] [PubMed]
  • Mickelsen PA, Blackman E, Sparling PF. Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from lactoferrin. Infect Immun. 1982 Mar;35(3):915–920. [PMC free article] [PubMed]
  • Mickelsen PA, Sparling PF. Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infect Immun. 1981 Aug;33(2):555–564. [PMC free article] [PubMed]
  • Miles AA, Khimji PL. Enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity. J Med Microbiol. 1975 Nov;8(4):477–490. [PubMed]
  • Muller-Eberhard U. Hemopexin. N Engl J Med. 1970 Nov 12;283(20):1090–1094. [PubMed]
  • Nagel RL, Gibson QH. The binding of hemoglobin to haptoglobin and its relation to subunit dissociation of hemoglobin. J Biol Chem. 1971 Jan 10;246(1):69–73. [PubMed]
  • Neilands JB. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. [PubMed]
  • Neilands JB. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. [PubMed]
  • Waalwijk C, van den Bosch JF, MacLaren DM, de Graaff J. Hemolysin plasmid coding for the virulence of a nephropathogenic Escherichia coli strain. Infect Immun. 1982 Jan;35(1):32–37. [PMC free article] [PubMed]
  • Weinberg ED. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. [PMC free article] [PubMed]
  • WHITE DC, GRANICK S. HEMIN BIOSYNTHESIS IN HAEMOPHILUS. J Bacteriol. 1963 Apr;85:842–850. [PMC free article] [PubMed]
  • Williams PH, Warner PJ. ColV plasmid-mediated, colicin V-independent iron uptake system of invasive strains of Escherichia coli. Infect Immun. 1980 Aug;29(2):411–416. [PMC free article] [PubMed]

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