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Infect Immun. 1986 May; 52(2): 349–355.
PMCID: PMC261005

Effect of hemin on the physiology and virulence of Bacteroides gingivalis W50.


Bacteroides gingivalis W50 was grown in a chemostat under steady-state conditions at pH 7.5 +/- 0.2 and a constant growth rate of 6.9 h for periods of up to 6 weeks (146 bacterial generations) in a complex medium. Hemin was capable of limiting the growth of cells up to a concentration of approximately 0.5 micrograms/ml since higher concentrations of hemin did not increase cell yields; cells grew in the absence of exogenously added vitamin K1. Only a limited number of amino acids was metabolized during growth, but because none of these was totally depleted, the limiting nutrient under hemin excess conditions was probably a peptide. A range of fermentation products was produced under all conditions of growth; higher concentrations of cytotoxic metabolites such as propionate and butyrate were formed under hemin excess conditions, although more ammonia was released under hemin limitation. When viewed by electron microscopy, cells grown under hemin limitation appeared to be either coccobacillary or short rods and possessed few fimbriae per cell, but large numbers of extracellular vesicles could be seen both surrounding the cell surface and free in the environment. In contrast, cells grown under hemin excess conditions were more commonly coccus shaped and were more heavily fimbriated but had fewer extracellular vesicles. Marked differences were found in the susceptibility of mice to infection with cells grown under different concentrations of hemin. Cells transferred to media without any added hemin were avirulent, whereas those grown under conditions of hemin limitation (0.33 and 0.40 micrograms/ml) produced a 20 and 50% mortality in mice, respectively. In contrast cells grown under hemin excess always caused 100% mortality in mice, although this virulence was dose dependent. When virulent, the bacteria caused an extensive, spreading infection with necrosis of the skin and subcutaneous tissues. Collagen disintegration was seen histologically, implying a role for collagenase production in the pathogenicity of these bacteria.

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Selected References

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  • Carlsson J, Höfling JF, Sundqvist GK. Degradation of albumin, haemopexin, haptoglobin and transferrin, by black-pigmented Bacteroides species. J Med Microbiol. 1984 Aug;18(1):39–46. [PubMed]
  • Duerden BI. The isolation and identification of Bacteroides spp. from the normal human gingival flora. J Med Microbiol. 1980 Feb;13(1):89–101. [PubMed]
  • Finn TM, Arbuthnott JP, Dougan G. Properties of Escherichia coli grown in vivo using a chamber implant system. J Gen Microbiol. 1982 Dec;128(12):3083–3091. [PubMed]
  • GIBBONS RJ, MACDONALD JB. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. [PMC free article] [PubMed]
  • Gregory EM, Fanning DD. Effect of heme on Bacteroides distasonis catalase and aerotolerance. J Bacteriol. 1983 Dec;156(3):1012–1018. [PMC free article] [PubMed]
  • Laughon BE, Syed SA, Loesche WJ. API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J Clin Microbiol. 1982 Jan;15(1):97–102. [PMC free article] [PubMed]
  • Loesche WJ, Syed SA. Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score. Infect Immun. 1978 Sep;21(3):830–839. [PMC free article] [PubMed]
  • Mayrand D, McBride BC. Exological relationships of bacteria involved in a simple, mixed anaerobic infection. Infect Immun. 1980 Jan;27(1):44–50. [PMC free article] [PubMed]
  • Mayrand D, McBride BC, Edwards T, Jensen S. Characterization of Bacteroides asaccharolyticus and B. melaninogenicus oral isolates. Can J Microbiol. 1980 Oct;26(10):1178–1183. [PubMed]
  • McKee AS, McDermid AS, Ellwood DC, Marsh PD. The establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula. J Appl Bacteriol. 1985 Sep;59(3):263–275. [PubMed]
  • Moore WE, Holdeman LV, Smibert RM, Hash DE, Burmeister JA, Ranney RR. Bacteriology of severe periodontitis in young adult humans. Infect Immun. 1982 Dec;38(3):1137–1148. [PMC free article] [PubMed]
  • Pittman KA, Lakshmanan S, Bryant MP. Oligopeptide uptake by Bacteroides ruminicola. J Bacteriol. 1967 May;93(5):1499–1508. [PMC free article] [PubMed]
  • Sciortino CV, Finkelstein RA. Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo. Infect Immun. 1983 Dec;42(3):990–996. [PMC free article] [PubMed]
  • Shah HN, Bonnett R, Mateen B, Williams RA. The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus. Biochem J. 1979 Apr 15;180(1):45–50. [PMC free article] [PubMed]
  • Shah HN, Williams RA, Bowden GH, Hardie JM. Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. J Appl Bacteriol. 1976 Dec;41(3):473–495. [PubMed]
  • Slots J. Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system. J Clin Microbiol. 1981 Sep;14(3):288–294. [PMC free article] [PubMed]
  • Slots J, Genco RJ. Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res. 1984 Mar;63(3):412–421. [PubMed]
  • Slots J, Gibbons RJ. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun. 1978 Jan;19(1):254–264. [PMC free article] [PubMed]
  • Socransky SS, Haffajee AD, Goodson JM, Lindhe J. New concepts of destructive periodontal disease. J Clin Periodontol. 1984 Jan;11(1):21–32. [PubMed]
  • Touw JJ, van Steenbergen TJ, De Graaff J. Butyrate: a cytotoxin for Vero cells produced by Bacteroides gingivalis and Bacteroides asaccharolyticus. Antonie Van Leeuwenhoek. 1982;48(4):315–325. [PubMed]
  • van Steenbergen TJ, Kastelein P, Touw JJ, de Graaff J. Virulence of black-pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice. J Periodontal Res. 1982 Jan;17(1):41–49. [PubMed]
  • Wahren A, Gibbons RJ. Amino acid fermentation by Bacteroides melaninogenicus. Antonie Van Leeuwenhoek. 1970;36(1):149–159. [PubMed]
  • Woo DD, Holt SC, Leadbetter ER. Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius. J Infect Dis. 1979 May;139(5):534–546. [PubMed]

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