• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Sep 1987; 55(9): 2032–2036.
PMCID: PMC260651

Protein and RNA synthesis by isolated Rickettsia prowazekii.

Abstract

Purified host-cell-free Rickettsia prowazekii synthesized protein from leucine, lysine, or proline as radioactive precursor. Most, if not all, of the rickettsial proteins were synthesized. The specific activities of the newly synthesized proteins in the cytoplasmic fraction and in the envelope fraction were not significantly different, indicating that both soluble and envelope proteins were synthesized and the latter were inserted into the envelope. The synthesis of protein was sensitive to chloramphenicol but insensitive to the eucaryotic inhibitors cycloheximide and atractyloside. When added before the initiation of synthetic activity, rifampin, a transcriptional inhibitor, inhibited protein synthesis as quickly and extensively as did chloramphenicol, a translational inhibitor. However, when added during synthetic activity, rifampin, unlike chloramphenicol, allowed protein synthesis to continue for several minutes. These results indicate that de novo transcription is necessary for de novo translation and that no stable mRNA pool exists in these rickettsiae. UMP was incorporated into both rRNA and mRNA. The minimal conditions necessary for RNA synthesis contrasted with the complex medium required for protein synthesis. The half-life of the labeled RNA synthesized in these rickettsiae was approximately 16 min. It was concluded that all the rickettsial mRNA that had existed in the growing intracellular rickettsiae was degraded during their lengthy purification.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (905K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BOVARNICK MR. Phosphorylation accompanying the oxidation of glutamate by the Madrid E strain of typhus rickettsiae. J Biol Chem. 1956 May;220(1):353–361. [PubMed]
  • Bovarnick MR. INCORPORATION OF ACETATE-1-C INTO LIPID BY TYPHUS RICKETTSIAE. J Bacteriol. 1960 Oct;80(4):508–512. [PMC free article] [PubMed]
  • BOVARNICK MR, MILLER JC, SNYDER JC. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. [PMC free article] [PubMed]
  • BOVARNICK MR, SCHNEIDER L. The incorporation of glycine-1-C14 by typhus rickettsiae. J Biol Chem. 1960 Jun;235:1727–1731. [PubMed]
  • BOVARNICK MR, SCHNEIDER L, WALTER H. The incorporation of labeled methionine by typhus rickettsiae. Biochim Biophys Acta. 1959 Jun;33(2):414–422. [PubMed]
  • Coolbaugh JC, Progar JJ, Weiss E. Enzymatic activities of cell-free extracts of Rickettsia typhi. Infect Immun. 1976 Jul;14(1):298–305. [PMC free article] [PubMed]
  • Hatch TP, Miceli M, Silverman JA. Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis. J Bacteriol. 1985 Jun;162(3):938–942. [PMC free article] [PubMed]
  • Krause DC, Winkler HH, Wood DO. Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12. Infect Immun. 1985 Jan;47(1):157–165. [PMC free article] [PubMed]
  • Krause DC, Winkler HH, Wood DO. Cloning and expression of the Rickettsia prowazekii ADP/ATP translocator in Escherichia coli. Proc Natl Acad Sci U S A. 1985 May;82(9):3015–3019. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Smith DK, Winkler HH. Characterization of a lysine-specific active transport system in Rickettsia prowazeki. J Bacteriol. 1977 Mar;129(3):1349–1355. [PMC free article] [PubMed]
  • Smith DK, Winkler HH. Separation of inner and outer membranes of Rickettsia prowazeki and characterization of their polypeptide compositions. J Bacteriol. 1979 Feb;137(2):963–971. [PMC free article] [PubMed]
  • Walker TS, Winkler HH. Penetration of cultured mouse fibroblasts (L cells) by Rickettsia prowazeki. Infect Immun. 1978 Oct;22(1):200–208. [PMC free article] [PubMed]
  • Weiss E. Growth and physiology of rickettsiae. Bacteriol Rev. 1973 Sep;37(3):259–283. [PMC free article] [PubMed]
  • Weiss E, Coolbaugh JC, Williams JC. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation. Appl Microbiol. 1975 Sep;30(3):456–463. [PMC free article] [PubMed]
  • Winkler HH. Rickettsial permeability. An ADP-ATP transport system. J Biol Chem. 1976 Jan 25;251(2):389–396. [PubMed]
  • Winkler HH. Early events in the interaction of the obligate intracytoplasmic parasite, Rickettsia prowazekii, with eucaryotic cells: entry and lysis. Ann Inst Pasteur Microbiol. 1986 May-Jun;137A(3):333–336. [PubMed]
  • Winkler HH, Daugherty RM. Proline transport and metabolism in Rickettsia prowazekii. J Bacteriol. 1984 May;158(2):460–463. [PMC free article] [PubMed]
  • Winkler HH, Daugherty RM. Acquisition of glucose by Rickettsia prowazekii through the nucleotide intermediate uridine 5'-diphosphoglucose. J Bacteriol. 1986 Sep;167(3):805–808. [PMC free article] [PubMed]
  • Winkler HH, Miller ET. Phospholipase A activity in the hemolysis of sheep and human erythrocytes by Rickettsia prowazeki. Infect Immun. 1980 Aug;29(2):316–321. [PMC free article] [PubMed]
  • Winkler HH, Miller ET. Phospholipase A and the interaction of Rickettsia prowazekii and mouse fibroblasts (L-929 cells). Infect Immun. 1982 Oct;38(1):109–113. [PMC free article] [PubMed]
  • Wood DO, Atkinson WH, Sikorski RS, Winkler HH. Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli. J Bacteriol. 1983 Jul;155(1):412–416. [PMC free article] [PubMed]
  • Zahorchak RJ, Winkler HH. Transmembrane electrical potential in Rickettsia prowazekii and its relationship to lysine transport. J Bacteriol. 1983 Feb;153(2):665–671. [PMC free article] [PubMed]
  • Zuerner RL, Thompson HA. Protein synthesis by intact Coxiella burnetii cells. J Bacteriol. 1983 Oct;156(1):186–191. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...