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Infect Immun. Jul 1987; 55(7): 1580–1587.
PMCID: PMC260561

Serotype specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae serotype 5.

Abstract

Serotyping of Haemophilus pleuropneumoniae and serologic assays for detection of serotype-specific antibody are problematic due to the potential cross-reactivity of the crude antigens used for raising immune serum or for serology. The capsular polymer (CP) of H. pleuropneumoniae serotype 5 was investigated for serotype-specific activity with antiserum to whole cells or with antiserum made monospecific to CP by adsorption with a capsule-deficient mutant. When antiserum to whole cells or monospecific antiserum to CP was tested against purified CP from serotypes 1 to 7 by immunodiffusion or enzyme-linked immunosorbent assay, only capsules of serotype 5 were reactive. In addition, only encapsulated serotype 5 cells reacted with serum monospecific to CP in an indirect immunofluorescent-antibody assay. Serotype-specific antibody was completely inhibited in each assay by preincubation of purified CP with the serum. Antiserum to whole cells of H. pleuropneumoniae serotype 5 contained antibodies to proteins and lipopolysaccharide; these antibodies cross-reacted with antigens of heterologous serotypes by dot-blot enzyme-linked immunosorbent assay and immunoblotting. The antigenic activity of CP was stable after heating for at least 30 min at 100 degrees C. High titers of antibody to CP were present in the sera of rabbits immunized intravenously with whole log-phase cells or in the convalescent sera of pigs experimentally infected with H. pleuropneumoniae serotype 5. However, the purified CP was poorly immunogenic in rabbits and swine. Our results indicate that the capsule is the serotype-specific antigen of H. pleuropneumoniae and that a monospecific antiserum to capsule or purified capsule should be used for serotyping or serologic assays, respectively.

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Selected References

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  • Afshar A, Wright PF, Dulac GC. Dot-enzyme immunoassay for visual detection of antibodies to pseudorabies virus in swine serum. J Clin Microbiol. 1986 Mar;23(3):563–567. [PMC free article] [PubMed]
  • Anderson P. Antibody responses to Haemophilus influenzae type b and diphtheria toxin induced by conjugates of oligosaccharides of the type b capsule with the nontoxic protein CRM197. Infect Immun. 1983 Jan;39(1):233–238. [PMC free article] [PubMed]
  • Anderson P, Smith DH. Isolation of the capsular polysaccharide from culture supernatant of Haemophilus influenzae type b. Infect Immun. 1977 Feb;15(2):472–477. [PMC free article] [PubMed]
  • Barenkamp SJ, Munson RS, Jr, Granoff DM. Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles. J Infect Dis. 1981 May;143(5):668–676. [PubMed]
  • Fenwick BW, Osburn BI, Olander HJ. Isolation and biological characterization of two lipopolysaccharides and a capsular-enriched polysaccharide preparation from Haemophilus pleuropneumoniae. Am J Vet Res. 1986 Jul;47(7):1433–1441. [PubMed]
  • Gunnarsson A. Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): extraction of type-specific antigens. Am J Vet Res. 1979 Apr;40(4):469–472. [PubMed]
  • Gunnarsson A. Evaluation of different antigens in the complement-fixation test for diagnosis of Haemophilus pleuropneumoniae (parahaemolyticus) infections in swine. Am J Vet Res. 1979 Nov;40(11):1564–1567. [PubMed]
  • Gunnarsson A, Biberstein EL, Hurvell B. Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): agglutination reactions. Am J Vet Res. 1977 Aug;38(8):1111–1114. [PubMed]
  • Gunnarsson A, Hurvell B, Biberstein EL. Serologic studies of porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): antigenic specificity and relationship between serotypes. Am J Vet Res. 1978 Aug;39(8):1286–1292. [PubMed]
  • Inzana TJ. Purification and partial characterization of the capsular polymer of Haemophilus pleuropneumoniae serotype 5. Infect Immun. 1987 Jul;55(7):1573–1579. [PMC free article] [PubMed]
  • Inzana TJ, Seifert WE, Jr, Williams RP. Composition and antigenic activity of the oligosaccharide moiety of Haemophilus influenzae type b lipooligosaccharide. Infect Immun. 1985 May;48(2):324–330. [PMC free article] [PubMed]
  • Mittal KR, Higgins R, Lariviere S. Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniae. J Clin Microbiol. 1982 Jun;15(6):1019–1023. [PMC free article] [PubMed]
  • Mittal KR, Higgins R, Lariviere S. Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test. J Clin Microbiol. 1983 May;17(5):787–790. [PMC free article] [PubMed]
  • Mittal KR, Higgins R, Larivière S. Identification and serotyping of Haemophilus pleuropneumoniae by coagglutination test. J Clin Microbiol. 1983 Dec;18(6):1351–1354. [PMC free article] [PubMed]
  • Nicolet J. Sur l'hémophilose du pore. 3. Différenciation sérologique de Haemophilus parahaemolyticus. Zentralbl Bakteriol Orig. 1971;216(4):487–495. [PubMed]
  • Nicolet J, Paroz P, Krawinkler M, Baumgartner A. An enzyme-linked immunosorbent assay, using an EDTA-extracted antigen for the serology of Haemophilus pleuropneumoniae. Am J Vet Res. 1981 Dec;42(12):2139–2142. [PubMed]
  • Nielsen R. Serological characterization of Haemophilus pleuropneumoniae (Actinobacillus pleuropneumoniae) strains and proposal of a new serotype: serotype 10. Acta Vet Scand. 1985;26(4):581–585. [PubMed]
  • Rapp VJ, Munson RS, Jr, Ross RF. Outer membrane protein profiles of Haemophilus pleuropneumoniae. Infect Immun. 1986 May;52(2):414–420. [PMC free article] [PubMed]
  • Rapp VJ, Ross RF, Erickson BZ. Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine. Am J Vet Res. 1985 Jan;46(1):185–192. [PubMed]
  • Rapp VJ, Ross RF, Young TF. Characterization of Haemophilus spp. isolated from healthy swine and evaluation of cross-reactivity of complement-fixing antibodies to Haemophilus pleuropneumoniae and Haemophilus taxon "minor group". J Clin Microbiol. 1985 Dec;22(6):945–950. [PMC free article] [PubMed]
  • Robbins JB. Vaccines for the prevention of encapsulated bacterial diseases: current status, problems and prospects for the future. Immunochemistry. 1978 Nov;15(10-11):839–854. [PubMed]
  • Rosendal S, Boyd DA. Haemophilus pleuropneumoniae serotyping. J Clin Microbiol. 1982 Nov;16(5):840–843. [PMC free article] [PubMed]
  • Rosendal S, Lombin L, DeMoor J. Serotyping and detection of Haemophilus pleuropneumoniae by indirect fluorescent antibody technique. Can J Comp Med. 1981 Jul;45(3):271–274. [PMC free article] [PubMed]
  • Rosendal S, Mittal KR. Serological cross-reactivity between a porcine Actinobacillus strain and Haemophilus pleuropneumoniae. Can J Comp Med. 1985 Apr;49(2):164–170. [PMC free article] [PubMed]
  • Schneerson R, Barrera O, Sutton A, Robbins JB. Preparation, characterization, and immunogenicity of Haemophilus influenzae type b polysaccharide-protein conjugates. J Exp Med. 1980 Aug 1;152(2):361–376. [PMC free article] [PubMed]
  • Schultz RA, Young TF, Ross RF, Jeske DR. Prevalence of antibodies to Haemophilus pleuropneumoniae in Iowa swine. Am J Vet Res. 1982 Oct;43(10):1848–1851. [PubMed]
  • Sebunya TN, Saunders JR. Haemophilus pleuropneumoniae infection in swine: a review. J Am Vet Med Assoc. 1983 Jun 15;182(12):1331–1337. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]

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