Logo of bmcgeneBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genetics
BMC Genet. 2008; 9: 74.
Published online Nov 19, 2008. doi:  10.1186/1471-2156-9-74
PMCID: PMC2605456

A second generation genetic map for rainbow trout (Oncorhynchus mykiss)

Abstract

Background

Genetic maps characterizing the inheritance patterns of traits and markers have been developed for a wide range of species and used to study questions in biomedicine, agriculture, ecology and evolutionary biology. The status of rainbow trout genetic maps has progressed significantly over the last decade due to interest in this species in aquaculture and sport fisheries, and as a model research organism for studies related to carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition. We constructed a second generation genetic map for rainbow trout using microsatellite markers to facilitate the identification of quantitative trait loci for traits affecting aquaculture production efficiency and the extraction of comparative information from the genome sequences of model fish species.

Results

A genetic map ordering 1124 microsatellite loci spanning a sex-averaged distance of 2927.10 cM (Kosambi) and having 2.6 cM resolution was constructed by genotyping 10 parents and 150 offspring from the National Center for Cool and Cold Water Aquaculture (NCCCWA) reference family mapping panel. Microsatellite markers, representing pairs of loci resulting from an evolutionarily recent whole genome duplication event, identified 180 duplicated regions within the rainbow trout genome. Microsatellites associated with genes through expressed sequence tags or bacterial artificial chromosomes produced comparative assignments with tetraodon, zebrafish, fugu, and medaka resulting in assignments of homology for 199 loci.

Conclusion

The second generation NCCCWA genetic map provides an increased microsatellite marker density and quantifies differences in recombination rate between the sexes in outbred populations. It has the potential to integrate with cytogenetic and other physical maps, identifying paralogous regions of the rainbow trout genome arising from the evolutionarily recent genome duplication event, and anchoring a comparative map with the zebrafish, medaka, tetraodon, and fugu genomes. This resource will facilitate the identification of genes affecting traits of interest through fine mapping and positional cloning of candidate genes.

Background

Genetic maps characterizing the inheritance patterns of traits and markers have been developed and utilized for a wide range of species and applications associated with studies addressing biomedical, agricultural, ecological and evolutionary questions. These applications often target the discovery of allelic variation affecting traits and have the eventual goal of identifying the exact DNA sequences underlying phenotypes [1]. Other studies highlight differences in recombination rates between the sexes as observed in the earliest linkage maps [2-4], or suggest mechanisms of chromosomal evolution through the identification of regions of conserved synteny across species. For instance, comparisons of conserved synteny and conserved gene orders have been conducted extensively within the vertebrates [5-8]. Whole genome sequences have enabled comparative genomic studies which employ computational approaches [9], including the identification of functional elements [10,11]. As a result, one additional goal for mapping in species not having access to a whole genome reference sequence is to develop high-density comparative maps with whole genome sequences of related species. This may be accomplished through genetic, cytogenetic, radiation hybrid, bacterial artificial chromosome (BAC), and integrated mapping approaches [12-16]. These maps not only enable the use of information across species, but may be used within a species to aid whole genome sequence assembly [17].

The construction of genetic maps for many species begins by genotyping reference families with markers such as microsatellites [18] which were initially developed for population genetic analyses. Microsatellites are often the marker of choice as they exhibit co-dominant inheritance, have high degrees of heterozygosity, are widely distributed throughout the genome, and may provide comparative information between closely related species[19]. When associated with a gene, these markers can provide comparative information across a great diversity of taxa. The limiting factors of microsatellites for map construction are the time and resources required for marker development and genotyping. Alternatively, amplified fragment length polymorphisms (AFLPs) [20] and random amplified polymorphic DNAs (RAPDs) [21] markers are inexpensive to develop and are conducive to high throughput genotyping protocols. Although large numbers of loci can be mapped rather inexpensively in a short amount of time, these markers are not associated with unique sequences and are specific to each mapping population. These efforts result in first generation linkage maps containing hundreds of markers which represent much of the genome with a low resolution of microsatellites [22-28]. As additional markers become available, including those associated with candidate genes, second generation maps containing several hundred to over one thousand markers spanning the entire genome at higher resolutions are constructed [29-38]. The ultimate genetic maps have sub-centiMorgan (cM) resolution and include anywhere from thousands to millions of markers [39-41]. Currently, high-density mapping efforts for human, model organism, and agriculturally important species with whole genome sequences are using single nucleotide polymorphism (SNP) [42] markers. Similar to microsatellites, SNPs are abundant, widely distributed throughout the genome, and are associated with a unique sequence. Although SNPs are amenable to genotyping with high-throughput protocols, they are less polymorphic and will require large numbers of crosses for mapping.

The status of rainbow trout genetic maps has progressed significantly over the last decade due to interest in their economic impacts as an aquaculture species and on sport fisheries, and as a model research organism for studies related to carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition [43]. Rainbow trout have a genome size estimated to be 2.4 × 109 bp [44]. While the karyotype of this species varies from 2 N = 58–64, the number of chromosome arms is conserved at 104. An evolutionarily recent whole genome duplication event is estimated to have occurred 25–100 mya [45], and the genome is estimated to be 1/3 of the way along the process of re-diploidization, [46]. Several laboratories have constructed genetic maps including AFLPs, microsatellites, and SNPs to identify quantitative trait loci (QTL) affecting time to hatch, development rate, growth, thermal tolerance, natural killer cell-like activity, albinism and disease resistance [47-56]. The first genetic map based on molecular markers was constructed by Young et al. [57] who observed the inheritance of 476 loci (332 AFLPs) on 76 doubled haploid fish. The resulting map contained 31 large linkage groups and spanned a total of 2627.5 cM. In 2000, Sakamoto et al. [19] mapped 208 loci (191 microsatellites) to 29 linkage groups by genotyping 186 fish from 3 backcross families. This effort revealed large recombination rate differences between the sexes (3.25:1 female to male) and a female map length over 1000 cM Morgans which is an underestimation as acknowledged by the authors. In 2003, Nichols et al. [58] added to the map of Young et al[57], ordering 1359 loci consisting primarily of 973 AFLPs and 226 microsatellites, and forming 30 large linkage groups with a map length of 4590 cM. Most recently, Guyomard et al. [59] used 120 offspring from two doubled haploid mitogynogenetic families to map 903 microsatellite loci with a map length of 2750 cM. Concurrently, Phillips et al. [60] integrated the cytogenetic and genetic maps by assigning linkage groups from Nichols et al[61] to specific chromosomes of the OSU doubled haploid line. Using microsatellites as comparative loci between salmonids, Danzmann et al. [62] added genetic markers to the map of Sakamoto et al. [19], reporting homeologous chromosome arm assignments within species resulting from the genome duplication event and pairwise homologous assignments between rainbow trout, artic char and Atlantic salmon.

In an effort to support the selective breeding of rainbow trout for aquaculture production efficiency, we constructed a genetic map to identify QTL affecting important traits and facilitate positional candidate cloning [63-66]. Most of the markers mapped were anonymous microsatellites from random enriched libraries, but our focus was to add markers with comparative information between the trout genome and other salmonids, and with the genome sequences of model fish species. By genotyping 30 offspring from each of 5 outbred families related to our broodstock germplasm, 1124 loci were ordered into 29 linkage groups representing each chromosome. This allowed for the observation of differences in recombination rate between the sexes and the creation of comparative maps. Anchoring of EST sequence on the genome sequences of other fishes has enabled the construction of comparative maps to facilitate genome research in regions of interest. The development and mapping of a large number of microsatellite loci will facilitate genome mapping efforts in rainbow trout and other salmonids.

Results

Genotyping

A total of 1435 microsatellite markers were developed or obtained from the literature including anonymous markers [19,59,61,67-80], markers developed in other salmonids [62,81-83], markers identified from BACs either containing genes or cytogenetic assignments [60,84], or markers representing expressed sequence tags (ESTs) and serving as comparative loci with sequenced genomes of model fish species [80,85] (see Additional File 1, Worksheet 1). In all, 930 new microsatellite markers were developed and mapped for this project. These markers were genotyped on the 10 parents and 150 offspring of the NCCCWA reference family panel. One hundred twenty three markers were scored as duplicates containing two sets of segregating alleles resulting in evaluation of 1558 loci. Of the 1435 markers attempted, 268 amplified poorly and were discarded. Another 87 were not informative in our reference families. A total of 1181 loci were informative for either the male or female map, with 1100 loci informative in the female and 1068 loci informative for the male; 991 loci were informative for both sexes (See Additional File 1, Worksheet 6). A total of 18 loci were observed to demonstrate pseudolinkage in the males [86], therefore only female data for those markers were included for linkage analysis.

Linkage Analysis

Two-point linkage analysis placed 1124 loci into 29 major linkage groups having 16 or more loci at LOD ≥ 10, an additional 56 were informative but were not localized to single linkage groups with significant LOD scores (See Additional File 1, Worksheet 10). The total combined sex averaged map distance was 2927.10 cM (Kosambi), a map representing OMY10 is presented in Figure Figure1,1, and maps representing all chromosomes are presented in Additional Files 2 (comparative map) and 3 (genetic map only). The numbers of markers included in a framework map created at LOD ≥ 4 was 137 and ranged from 0–9 markers per chromosome. Chromosomes OMY1, 5, 21, or 23 did not contain any framework markers. Additional loci were added at LOD ≥ 3 (343), ≥ 2 (67) ≥ 1 (30), and ≥ 0 (547). Additional File 1, Worksheet 10 contains this information and can be used to recreate maps using MapChart software [87]. The average resolution of the genome map was 2.6 cM with individual chromosome maps ranging from 1.64 to 4.09 cM. The map contains 1095 gaps, ~84% of which are less than 5 cM and ~94% are less than 10 cM (See Figure Figure2).2). The markers used to assign linkage groups to the cytogenetic map of Phillips et al. [60] are presented in Additional File 1, Worksheet 7 in addition to markers comparing linkage groups across genetic maps as modified from Guyomard et al. and Nichols et al. reported in Worksheet 8 [59,61]. Overall statistics for the map and each chromosome are given in Additional File 1, Worksheet 9 including chromosome, number of markers, number of markers from ESTs, number of markers from BACs, number of markers with homology assignments to genome sequences of 4 model fish species, number of markers ordered at each LOD threshold, total map length, female map length, male map length, female:male recombination ratios, recombination rate correlations and map resolution. The female:male recombination ratio was 1.68:1, with the female having a map length of 4,317.60 cM and the male map 2,564.10 cM. This ratio varied by chromosome, ranging from 0.73:1 to 12.22:1 (Kosambi) (See Table Table1).1). Figure Figure33 shows a map representing the differences in recombination rate between the sexes along the chromosome length for OMY16. Maps for the other chromosomes are presented in Additional File 4.

Table 1
Chromosome specific differences in recombination rates between sexes
Figure 1
Genetic map of rainbow trout chromosome 10 including comparative assignments. Recombination distances are presented as total Kosambi cM for each map on the left, marker names and comparative assignments are on the right. Loci names in red bold font are ...
Figure 2
Gaps in the rainbow trout genetic map. The NCCCWA Genetic map has an average resolution of one marker per 2.6 cM and contains 1095 gaps, ~84% of which are less than 5 cM and ~94% are less than 10 cM. Figure 2 shows the number of gaps observed in the NCCCWA ...
Figure 3
Chromosome specific variation in recombination rate between sexes. The genetic map of OMY16 is presented on the left, on the right a chart of male (blue line) and female (red line) recombination fractions between each pair of loci. All chromosomes are ...

Homeologous Assignments

The frequency of duplicated microsatellite loci in rainbow trout is very high as a result of the recent salmonid whole genome duplication. We were able to score two loci for 123 markers, denoting the loci names with a lower case "a" or "b." Although two loci were informative for all 123 of these markers, in many cases only one locus was successfully ordered on the map. Our map revealed 180 duplicated marker regions, including 30 pairs of homeologues from ESTs and 10 pairs identified from BACs that harbor genes of interest (Figures (Figures4,4, ,5,5, ,6).6). In addition, 149 loci are potential duplicates with monomorphic amplicons at a putative second locus.

Figure 4
Comparative maps. The rainbow trout genetic map serves as a comparative map within its own genome and with the genome sequences of zebrafish (DRE), medaka (OLA), and tetraodon (TNI). Assignments for fugu scaffolds are reported in Additional File 1 Worksheets ...
Figure 5
Comparative maps. The rainbow trout genetic map serves as a comparative map within its own genome and with the genome sequences of zebrafish (DRE), medaka (OLA), and tetraodon (TNI). Assignments for fugu scaffolds are reported in Additional File 1 Worksheets ...
Figure 6
Comparative maps. The rainbow trout genetic map serves as a comparative map within its own genome and with the genome sequences of zebrafish (DRE), medaka (OLA), and tetraodon (TNI). Assignments for fugu scaffolds are reported in Additional File 1 Worksheets ...

Comparative Assignments

The ability to use microsatellites developed from one salmonid in other salmonid species provides comparative mapping information. To this end, our map includes 33 markers from Atlantic salmon, 8 from sockeye salmon, 3 from pink salmon, and 1 from Chinook salmon. In addition to comparative maps with the salmonids, our map includes markers representing 325 ESTs and 57 loci from BACs that harbor genes of interest. These have the potential to serve in developing comparative maps with the genome sequences of model fish species. Additional File 1, Worksheet 2 contains comparative assignments of homology for markers developed from ESTs, which have markers names in the OMM5000 series or GenBank accession numbers. Two strategies were used to assign functional annotation to these markers. First, Worksheet 3 contains functional annotation for those markers derived directly from blastx hits. Secondly, Worksheet 4 identifies the corresponding Unigene [88] or Rainbow Trout Gene Index [89] record, including the EST from which each marker was designed. Functional annotation through BLAST and GO assignments are available through these resources. Worksheet 5 contains assignments of comparative homology for markers derived from BACs that contain genes of interest. These marker names are in the OMM3000 series. Homologs for 199 loci were identified in zebrafish (146), medaka (123), tetraodon (164), or fugu (131) (Figures (Figures4,4, ,5,5, ,66).

Discussion

Map Overview

The NCCCWA genetic map of rainbow trout was constructed by observing the inheritance of 1124 microsatellite markers in 5 families containing 30 offspring each. Although all linkage groups were identified with a high level of confidence, many markers were ordered at low LOD scores, primarily the result of a low number of informative meiosis. This was especially true for most duplicated markers where only one family with a maximum of 30 offspring could be scored. The map contains many markers which provide comparative information by identifying regions of homeology within the trout genome or regions of conserved synteny with genome sequences of model fish species. We observed whole genome map lengths of 4317.6 cM and 2564.1 cM for females and males, respectively, which is similar to the distances reported by Young et al. (2627.5 cM)[90], Nichols et al. (4590 cM) [61], and Guyomard et al. (2750 cM) [59]. However, our female map length differs significantly from the 10 Morgans reported by Sakamoto et al. [19] who reported the differences in sex recombination ratio to be 3.25:1. We observed an average sex recombination ratio of 1.68:1, but it varied greatly by chromosome and sub-chromosomal region. One explanation is that the microsatellites used in our map and the AFLPs used in the previous map differ with respect to their co-location with recombination hot spots. Another explanation is likely due to the marker densities on specific linkage groups which show higher ratios than the rest of the map. The chromosome specific ratios of 12.22:1, 4.58:1, 7.97:1, and 6.14:1, observed for chromosomes OMY 1, 15, 23, and 26, respectively, are well outside of the range for the rest of the chromosomes (.73:1 – 2.77:1). Having less drastic difference in this ratio than observed by Sakamoto et al. [19] facilitated the construction of a sex-averaged map in which we could include loci informative in any one of the 10 parents in the NCCCWA mapping reference families. However, the differences in recombination ratios are significant and sex should be accounted for when designing QTL experiments.

Genome Duplication

Due to the evolutionarily recent whole genome duplication event, many microsatellite markers in salmonids exist as two copies in the genome, frequently resulting in two loci which can be genotyped per primer set [19,43,45,46,78,80,91]. In some instances the two loci can be distinguished due to drastic differences in allele sizes, but more often the loci have overlapping and identical allele sizes and include null alleles. In the latter case, the loci often can be scored in only one family, reducing the observed number of informative meioses supporting map construction. The benefit of these markers is that they identify chromosome fragments that probably share a common ancestor, and are likely to have similar complements of genes in various states of re-diploidization. As presented in Figures Figures4,4, ,5,5, ,6,6, we identified 180 assignments of homeology in the rainbow trout genome. As observed previously [19,59,61,62], several chromosomes showed homeology primarily with one other chromosome, including the pairs OMY1/OMY23, OMY8/OMY28, OMY10/OMY19, OMY2/OMY3, OMY15/OMY21, OMY16/OMY20, OMY6/OMY26, OMY7/OMY18, and OMY13/OMY17. Chromosomes OMY2, OMY6, OMY19, OMY25, and OMY27 showed regions of homeology within the chromosome. The mapping of duplicated microsatellites from BACs and ESTs suggests that the gene complements of these regions may be similar and is useful for comparative mapping these regions with other salmonids and with the genomes of model fish species.

Comparative Assignments

Through the development of microsatellite markers from 325 ESTs and 57 BACs, we identified homologs for 199 loci in zebrafish, medaka, tetraodon, and/or fugu for the construction of a comparative map. Assignments include 146 for zebrafish, 123 for medaka, 164 for tetraodon, and 131 for fugu. As the fugu genome is not fully assembled, we report comparative assignments only for zebrafish, medaka, and tetraodon in Figures Figures4,4, ,5,5, ,6.6. There were 34, 30, and 22 comparative assignments for zebrafish, medaka, and tetraodon, respectively, where more than 2 markers from the same chromosome were assigned to the same rainbow trout chromosome. There were 29, 26, and 17 blocks of conserved synteny as defined by two or more consecutive assignments from the same chromosome for zebrafish, medaka, and tetraodon, respectively. These assignments of homology will facilitate candidate gene discovery, potentially providing comparative genome sequence information to marker intervals of interest (e.g. from QTL detection experiments).

Conclusion

This second generation NCCCWA rainbow trout genetic map provides an increased microsatellite marker density, estimates of sex specific recombination rates across the genome of outbred populations and a framework for producing an integrated genetic and physical map. The map identifies paralogous regions of the rainbow trout genome arising from the evolutionarily recent salmonid genome duplication, and serves as a starting point for comparative maps with the zebrafish, medaka, tetraodon, and fugu genomes. This resource will facilitate the identification of genes affecting traits of interest through fine mapping and positional candidate cloning.

Methods

Reference Family Panel

Reference families for mapping studies were selected from the National Center for Cool and Cold Water Aquaculture's 2002 brood year including 10 parental fish originating from the following strains: Clear Spring (CS), Troutlodge (TL), and Donaldson from the University of Washington (UW) [92]. The majority of karyotypes for fish related to the parents were determined to have 2 N = 58 chromosomes, with low frequencies of variation of up to 2 N = 64. Parental fin clips and 30 offspring from each mating, including one intra-strain cross (CS × CS) and 4 inter-strain crosses (2 TL × UW, 2 UW × TL), were sampled for DNA extractions using the phenol-chloroform method described in Sambrook and Russell [93]. DNA samples were quantified by spectrophotometer (Beckman DU 640, Beckman Instruments, St. Louis, MO, USA) and diluted to a concentration of 12.5 ng/ul for PCR.

Microsatellite Genotyping

A total of 1435 microsatellite markers were developed or obtained from the literature including anonymous markers [19,59,61,67-80], markers developed in other salmonids [62,81-83], markers identified from BACs either containing genes or cytogenetic assignments [60,84,94-96], or markers representing expressed sequence tags (ESTs) and serving as comparative loci with sequenced genomes of model fish species [80,85]. Marker information including locus names, optimum annealing temperatures and magnesium concentrations, GenBank accession numbers, and primer sequences are reported in Additional File 1, Worksheet 1. Markers were either genotyped using the tailed protocol of Boutin-Ganache et al. [97] or by direct fluorescent labelling (with FAM, HEX, or NED) of the forward primer according to manufacturer protocols (ABI, Foster City, CA, USA). Primer pairs were obtained from commercial sources (forward primers labelled with FAM or HEX from Alpha DNA, Montreal, Quebec, Canada, or NED from ABI, Foster City, CA, USA). PCR reactions consisted of 12 μl reaction volumes containing 12.5 ng DNA, 1.5–2.5 mM MgCl2, 1.0 μM of each primer, 200 μM of dNTPs, 1× manufacturer's reaction buffer and 0.5 units Taq DNA polymerase. Thermal cycling consisted of an initial denaturation at 95°C for 15 min followed by 30 cycles of 95°C for 1 min, annealing temperature for 45 s, 72°C extension for 45 s, then a final extension at 72°C for 10 min. PCR products were visualized on agarose gels after staining with ethidium bromide. Markers were grouped in combinations of two or three markers based on differences in fluorescent dye color and amplicon size. Three μl of each PCR product was added to 20 μl of water, 1 μl of the diluted sample was added to 12.5 μl of loading mixture made up with 12 μl of HiDi formamide and 0.5 of Genscan 400 ROX internal size standard. Samples were denatured at 95°C for 5 min and kept on ice until loading on an automated DNA sequencer ABI 3730 DNA Analyzer (ABI, Foster City, CA, USA). Output files were analyzed using GeneMapper version 3.7 (ABI, Foster City, CA, USA), formatted using Microsoft Excel and stored in Microsoft Access. As a result of the evolutionarily recent genome duplication, microsatellite markers in salmonids are often present in two copies in the genome, each copy potentially having overlapping allele size ranges and possibly including alleles having identical sizes. Markers which were duplicated were scored as independent loci, adding an "a" and "b" to differentiate their locus names. Duplicated loci with overlapping and/or identical allele sizes were scored only in the family containing the most informative meiosis.

Linkage Analyses

Genotype data combined for both sexes were formatted using MAKEPED of the LINKAGE [98] program and checked for inconsistencies with Mendelian inheritance using PEDCHECK [99]. RECODE [100] and LNKTOCRI [101] were used to assemble the data into CRIMAP [102] format. MULTIMAP [103] was used to conduct two-point and multi-point linkage analyses. Two-point linkage analysis included parameters of LOD ≥ 10 and recombination fraction r ≤ 0.5. Multipoint linkage analysis was conducted on individual linkage groups, including loci unlinked at LOD ≥ 10 but linked to loci in that linkage group at LOD ≥ 4. Framework maps were constructed using default parameters, markers were added to comprehensive maps by lowering the LOD threshold one integer at a time and starting with the previous order. Resulting maps are consensus maps, accounting for co-informative meiosis across the five families.

Linkage Group Nomenclature

Linkage groups were assigned chromosome names using the integrated cytogenetic/linkage map of Phillips et al. [60]. Specific markers used to identify cytogenetic chromosome names are listed in Additional File 1 Worksheet 7 (Markers for Map Integration). In an effort to identify common linkage groups between published maps, Additional File 1 Worksheet 8 (Linkage Group Translation) has been adapted from Guyomard et al. [59].

Estimating Differences in Recombination Rates between the Sexes

Multimap reports sex averaged, female and male recombination rates for any given map order. Whole-genome map lengths were obtained by adding the total cM for each chromosome for the sex-averaged, female, and male maps. To estimate the genome wide female:male recombination ratio, the entire map length for the female was divided by that of the male. To evaluate chromosome specific rates, pairwise distances in cM between adjacent map intervals were calculated and presented in Figure Figure33 and Additional File 4, chromosome specific ratios are reported in Additional File 1 Worksheet 9 (Chromosome Information).

Comparative Assignments

Expressed Sequences associated with microsatellites (OMM5000 and GenBank accession no. designations) for markers were BLASTed [104] using blastn against the transcripts of each genome obtained from http://www.ensembl.org. Only matches having a minimum alignment length over 50 bp and percent identity over 78% were treated as potential matches. Data were hand checked and assignments which were questionable were removed. Microsatellites identified from bacterial artificial chromosomes were annotated with genes known to be contained within those clones by sequence analysis.

Authors' contributions

CER designed the study, collected genotypes and conducted the linkage analysis. RV developed the linkage analysis pipeline and participated in linkage analysis, YP participated in marker development and genotyping, SAG participated in marker development. Dr. Jeffrey Silverstein participated in cross design and execution.

Supplementary Material

Additional file 1:

Marker and Mapping Information. Additional Files are included as numbered spreadsheets in a MS Excel file:

1 Marker PCR Information (PCR primers, conditions, etc...)

2 ESTs – Comparative Assignments (identification of homologs of OMM5000 markers, developed from ESTs, in the zebrafish, medaka, tetraodon, and fugu genomes)

3 ESTs – BLASTx Annotation (Functional annotation for EST markers assigned through blastx of the GenBank nr database)

4 ESTs – RTGI and Unigene (Identification of Rainbow Trout Gene Index and Unigene Assemblies for which EST markers belong)

5 Comparative Info – BACs (Comparative information for OMM3000 markers derived from BACs containing genes of interest)

6 Inf Mei and Allele#s (informative meiosis and number of alleles for markers screened on the NCCCWA reference families)

7 Markers for Map Integration (Markers used to integrate NCCCWA linkage groups and the rainbow trout cytogenetic map)

8 Linkage Group Translation (identifying names of common linkage groups between published rainbow trout genetic maps)

9 Chromosome Info (Linkage analysis information broken down by chromosome)

10 2pt Info for loci informative but not mapped (Reporting loci which were informative in the NCCCWA reference families but not able to be placed on the map)

11 Maps (Genetic map data which can be used to recreate map figues in MapChart)

Additional file 2:

Genetic Map. This Adobe PDF file includes figures representing the 29 linkage groups/chromosomes of the NCCCWA rainbow trout genetic map. Recombination distances are presented as total Kosambi cM for each map on the left, marker names and comparative assignments are on the right. Loci names in red bold font are ordered at LOD 4.0, loci in blue font at LOD 3.0, loci in green font at LOD 2.0, loci in black font at LOD 1.0 and loci in black italic at LOD 0.0. Paralogous assignments within the rainbow trout genome are presented in orange in the format Omy_1:10.0 where Omy is the three letter species designation for rainbow trout (Oncorhynchus mykiss), 1 is the chromosome number of the paralogous locus and 10.0 is its map position. Comparative assignments are similarly presented next to loci names in the format species abbreviation_chromosome number:homolog start nucleotide:homolog end nucleotide. Comparative assignments for zebrafish are presented in purple, for medaka in brown, for tetraodon in light blue, and fugu in maroon.

Additional file 3:

Genetic Map. This Adobe PDF file includes figures representing the 29 linkage groups/chromosomes of the NCCCWA rainbow trout genetic map. Recombination distances are presented as total Kosambi cM for each map on the left, marker names are on the right. Loci names in red bold font are ordered at LOD 4.0, loci in blue font at LOD 3.0, loci in green font at LOD 2.0, loci in black font at LOD 1.0 and loci in black italic at LOD 0.0.

Additional file 4:

Sex Recombination Ratios. This Adobe PDF file includes figures representing the 29 linkage groups/chromosomes of the NCCCWA rainbow trout genetic map and figures representing the differences in recombination rate between the sexes along each chromosome. The map length is presented in terms of Kosambi cM, the pairwise recombination fractions (theta) between each marker pair along the length of the chromosome map is presented.

Acknowledgements

The authors wish to acknowledge Roseanna Long, Kristy Shewbridge, and M. Renee Fincham for their excellent technical expertise, Dr. Jeffrey Silverstein for providing the crosses, Dr. Ruth Phillips for ongoing collaboration on physical/genetic map integration, and Dr. Thomas Kocher, Dr. Roy Danzmann and Dr. Gary Rohrer for providing expert advice on linkage analysis.

References

  • Lander ES, Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989;121:185–199. [PMC free article] [PubMed]
  • Haldane J. Sex ratio and unisexual sterility in hybrid animals. Journal of Genetics. 1922;12:101–109. doi: 10.1007/BF02983075. [Cross Ref]
  • Huxley J. Sexual difference of linkage in Gammarus cheyreuxi. Journal of Genetics. 1928;20:145–156. doi: 10.1007/BF02983136. [Cross Ref]
  • Dunn L, Bennett D. Sex differences in recombination of linked genes in animals. Genetic Research. 1967;9:211–220. [PubMed]
  • O'Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG. Anchored reference loci for comparative genome mapping in mammals. Nat Genet. 1993;3:103–112. doi: 10.1038/ng0293-103. [PubMed] [Cross Ref]
  • Burt DW. Comparative mapping in farm animals. Brief Funct Genomic Proteomic. 2002;1:159–168. doi: 10.1093/bfgp/1.2.159. [PubMed] [Cross Ref]
  • Nadeau JH. Maps of linkage and synteny homologies between mouse and man. Trends Genet. 1989;5:82–86. doi: 10.1016/0168-9525(89)90031-0. [PubMed] [Cross Ref]
  • Serikawa T, Cui Z, Yokoi N, Kuramoto T, Kondo Y, Kitada K, Guenet JL. A comparative genetic map of rat, mouse and human genomes. Exp Anim. 1998;47:1–9. doi: 10.1538/expanim.47.1. [PubMed] [Cross Ref]
  • Miller W, Rosenbloom K, Hardison RC, Hou M, Taylor J, Raney B, Burhans R, King DC, Baertsch R, Blankenberg D, et al. 28-way vertebrate alignment and conservation track in the UCSC Genome Browser. Genome Res. 2007;17:1797–1808. doi: 10.1101/gr.6761107. [PMC free article] [PubMed] [Cross Ref]
  • Kheradpour P, Stark A, Roy S, Kellis M. Reliable prediction of regulator targets using 12 Drosophila genomes. Genome Res. 2007;17:1919–1931. doi: 10.1101/gr.7090407. [PMC free article] [PubMed] [Cross Ref]
  • Siepel A, Diekhans M, Brejova B, Langton L, Stevens M, Comstock CL, Davis C, Ewing B, Oommen S, Lau C, et al. Targeted discovery of novel human exons by comparative genomics. Genome Res. 2007;17:1763–1773. doi: 10.1101/gr.7128207. [PMC free article] [PubMed] [Cross Ref]
  • Breen M, Hitte C, Lorentzen TD, Thomas R, Cadieu E, Sabacan L, Scott A, Evanno G, Parker HG, Kirkness EF, et al. An integrated 4249 marker FISH/RH map of the canine genome. BMC Genomics. 2004;5:65. doi: 10.1186/1471-2164-5-65. [PMC free article] [PubMed] [Cross Ref]
  • Kayang BB, Fillon V, Inoue-Murayama M, Miwa M, Leroux S, Feve K, Monvoisin JL, Pitel F, Vignoles M, Mouilhayrat C, et al. Integrated maps in quail (Coturnix japonica) confirm the high degree of synteny conservation with chicken (Gallus gallus) despite 35 million years of divergence. BMC Genomics. 2006;7:101. doi: 10.1186/1471-2164-7-101. [PMC free article] [PubMed] [Cross Ref]
  • Snelling WM, Gautier M, Keele JW, Smith TP, Stone RT, Harhay GP, Bennett GL, Ihara N, Takasuga A, Takeda H, et al. Integrating linkage and radiation hybrid mapping data for bovine chromosome 15. BMC Genomics. 2004;5:77. doi: 10.1186/1471-2164-5-77. [PMC free article] [PubMed] [Cross Ref]
  • Kitada K, Voigt B, Kondo Y, Serikawa T. An integrated rat genome map based on genetic and cytogenetic data. Exp Anim. 2000;49:119–126. doi: 10.1538/expanim.49.119. [PubMed] [Cross Ref]
  • Mellersh CS, Hitte C, Richman M, Vignaux F, Priat C, Jouquand S, Werner P, Andre C, DeRose S, Patterson DF, et al. An integrated linkage-radiation hybrid map of the canine genome. Mamm Genome. 2000;11:120–130. doi: 10.1007/s003350010024. [PubMed] [Cross Ref]
  • Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, et al. A second generation radiation hybrid map to aid the assembly of the bovine genome sequence. BMC Genomics. 2006;7:283. doi: 10.1186/1471-2164-7-283. [PMC free article] [PubMed] [Cross Ref]
  • Weber JL, May PE. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet. 1989;44:388–396. [PMC free article] [PubMed]
  • Sakamoto T, Danzmann RG, Gharbi K, Howard P, Ozaki A, Khoo SK, Woram RA, Okamoto N, Ferguson MM, Holm LE, et al. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics. 2000;155:1331–1345. [PMC free article] [PubMed]
  • Vos P, Hogers R, Bleeker M, Reijans M, Lee T van de, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995;23:4407–4414. doi: 10.1093/nar/23.21.4407. [PMC free article] [PubMed] [Cross Ref]
  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18:6531–6535. doi: 10.1093/nar/18.22.6531. [PMC free article] [PubMed] [Cross Ref]
  • Dawson E, Abecasis GR, Bumpstead S, Chen Y, Hunt S, Beare DM, Pabial J, Dibling T, Tinsley E, Kirby S, et al. A first-generation linkage disequilibrium map of human chromosome 22. Nature. 2002;418:544–548. doi: 10.1038/nature00864. [PubMed] [Cross Ref]
  • Kayang BB, Vignal A, Inoue-Murayama M, Miwa M, Monvoisin JL, Ito S, Minvielle F. A first-generation microsatellite linkage map of the Japanese quail. Anim Genet. 2004;35:195–200. doi: 10.1111/j.1365-2052.2004.01135.x. [PubMed] [Cross Ref]
  • Khatkar MS, Collins A, Cavanagh JA, Hawken RJ, Hobbs M, Zenger KR, Barris W, McClintock AE, Thomson PC, Nicholas FW, Raadsma HW. A first-generation metric linkage disequilibrium map of bovine chromosome 6. Genetics. 2006;174:79–85. doi: 10.1534/genetics.106.060418. [PMC free article] [PubMed] [Cross Ref]
  • Lallias D, Beaumont AR, Haley CS, Boudry P, Heurtebise S, Lapegue S. A first-generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers. Anim Genet. 2007;38:560–568. doi: 10.1111/j.1365-2052.2007.01611.x. [PubMed] [Cross Ref]
  • Samollow PB, Kammerer CM, Mahaney SM, Schneider JL, Westenberger SJ, VandeBerg JL, Robinson ES. First-generation linkage map of the gray, short-tailed opossum, Monodelphis domestica, reveals genome-wide reduction in female recombination rates. Genetics. 2004;166:307–329. doi: 10.1534/genetics.166.1.307. [PMC free article] [PubMed] [Cross Ref]
  • Swinburne J, Gerstenberg C, Breen M, Aldridge V, Lockhart L, Marti E, Antczak D, Eggleston-Stott M, Bailey E, Mickelson J, et al. First comprehensive low-density horse linkage map based on two 3-generation, full-sibling, cross-bred horse reference families. Genomics. 2000;66:123–134. doi: 10.1006/geno.2000.6207. [PubMed] [Cross Ref]
  • Tobler A, Kapan D, Flanagan NS, Gonzalez C, Peterson E, Jiggins CD, Johntson JS, Heckel DG, McMillan WO. First-generation linkage map of the warningly colored butterfly Heliconius erato. Heredity. 2005;94:408–417. doi: 10.1038/sj.hdy.6800619. [PubMed] [Cross Ref]
  • Cox LA, Mahaney MC, Vandeberg JL, Rogers J. A second-generation genetic linkage map of the baboon (Papio hamadryas) genome. Genomics. 2006;88:274–281. doi: 10.1016/j.ygeno.2006.03.020. [PubMed] [Cross Ref]
  • de Gortari MJ, Freking BA, Cuthbertson RP, Kappes SM, Keele JW, Stone RT, Leymaster KA, Dodds KG, Crawford AM, Beattie CW. A second-generation linkage map of the sheep genome. Mamm Genome. 1998;9:204–209. doi: 10.1007/s003359900726. [PubMed] [Cross Ref]
  • Guerin G, Bailey E, Bernoco D, Anderson I, Antczak DF, Bell K, Biros I, Bjornstad G, Bowling AT, Brandon R, et al. The second generation of the International Equine Gene Mapping Workshop half-sibling linkage map. Anim Genet. 2003;34:161–168. doi: 10.1046/j.1365-2052.2003.00973.x. [PubMed] [Cross Ref]
  • Joobeur T, Periam N, de Vicente MC, King GJ, Arus P. Development of a second generation linkage map for almond using RAPD and SSR markers. Genome. 2000;43:649–655. doi: 10.1139/gen-43-4-649. [PubMed] [Cross Ref]
  • Kappes SM, Keele JW, Stone RT, McGraw RA, Sonstegard TS, Smith TP, Lopez-Corrales NL, Beattie CW. A second-generation linkage map of the bovine genome. Genome Res. 1997;7:235–249. doi: 10.1101/gr.7.3.235. [PubMed] [Cross Ref]
  • Lee BY, Lee WJ, Streelman JT, Carleton KL, Howe AE, Hulata G, Slettan A, Stern JE, Terai Y, Kocher TD. A second-generation genetic linkage map of tilapia (Oreochromis spp.) Genetics. 2005;170:237–244. doi: 10.1534/genetics.104.035022. [PMC free article] [PubMed] [Cross Ref]
  • Matise TC, Chen F, Chen W, De La Vega FM, Hansen M, He C, Hyland FC, Kennedy GC, Kong X, Murray SS, et al. A second-generation combined linkage physical map of the human genome. Genome Res. 2007;17:1783–1786. doi: 10.1101/gr.7156307. [PMC free article] [PubMed] [Cross Ref]
  • Neff MW, Broman KW, Mellersh CS, Ray K, Acland GM, Aguirre GD, Ziegle JS, Ostrander EA, Rine J. A second-generation genetic linkage map of the domestic dog, Canis familiaris. Genetics. 1999;151:803–820. [PMC free article] [PubMed]
  • Paglia GP, Olivieri AM, Morgante M. Towards second-generation STS (sequence-tagged sites) linkage maps in conifers: a genetic map of Norway spruce (Picea abies K.) Mol Gen Genet. 1998;258:466–478. doi: 10.1007/s004380050757. [PubMed] [Cross Ref]
  • Weissenbach J. A second generation linkage map of the human genome based on highly informative microsatellite loci. Gene. 1993;135:275–278. doi: 10.1016/0378-1119(93)90077-G. [PubMed] [Cross Ref]
  • Altshuler D, Pollara VJ, Cowles CR, Van Etten WJ, Baldwin J, Linton L, Lander ES. An SNP map of the human genome generated by reduced representation shotgun sequencing. Nature. 2000;407:513–516. doi: 10.1038/35035083. [PubMed] [Cross Ref]
  • Frazer KA, Ballinger DG, Cox DR, Hinds DA, Stuve LL, Gibbs RA, Belmont JW, Boudreau A, Hardenbol P, Leal SM, et al. A second generation human haplotype map of over 3.1 million SNPs. Nature. 2007;449:851–861. doi: 10.1038/nature06258. [PMC free article] [PubMed] [Cross Ref]
  • Frazer KA, Eskin E, Kang HM, Bogue MA, Hinds DA, Beilharz EJ, Gupta RV, Montgomery J, Morenzoni MM, Nilsen GB, et al. A sequence-based variation map of 8.27 million SNPs in inbred mouse strains. Nature. 2007;448:1050–1053. doi: 10.1038/nature06067. [PubMed] [Cross Ref]
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980;32:314–331. [PMC free article] [PubMed]
  • Thorgaard GH, Bailey GS, Williams D, Buhler DR, Kaattari SL, Ristow SS, Hansen JD, Winton JR, Bartholomew JL, Nagler JJ, et al. Status and opportunities for genomics research with rainbow trout. Comp Biochem Physiol B Biochem Mol Biol. 2002;133:609–646. doi: 10.1016/S1096-4959(02)00167-7. [PubMed] [Cross Ref]
  • Ohno S, Atkin NB. Comparative DNA values and chromosome complements of eight species of fishes. Chromosoma. 1966;18:455–466. doi: 10.1007/BF00332549. [PubMed] [Cross Ref]
  • Allendorf FW, Thorgaard GH. Tetraploidy and the evolution of salmonid fishes. In: Turner BJ, editor. Evolutionary Genetics of Fishes. New York: Plenum Press; 1984. pp. 1–46.
  • Palti Y, Gahr SA, Hansen JD, Rexroad CE., 3rd Characterization of a new BAC library for rainbow trout: evidence for multi-locus duplication. Anim Genet. 2004;35:130–133. doi: 10.1111/j.1365-2052.2004.01112.x. [PubMed] [Cross Ref]
  • Leder EH, Danzmann RG, Ferguson MM. The candidate gene, Clock, localizes to a strong spawning time quantitative trait locus region in rainbow trout. J Hered. 2006;97:74–80. doi: 10.1093/jhered/esj004. [PubMed] [Cross Ref]
  • Martinez V, Thorgaard G, Robison B, Sillanpaa MJ. An application of Bayesian QTL mapping to early development in double haploid lines of rainbow trout including environmental effects. Genet Res. 2005;86:209–221. doi: 10.1017/S0016672305007871. [PubMed] [Cross Ref]
  • Nichols KM, Broman KW, Sundin K, Young JM, Wheeler PA, Thorgaard GH. Quantitative trait loci × maternal cytoplasmic environment interaction for development rate in Oncorhynchus mykiss. Genetics. 2007;175:335–347. doi: 10.1534/genetics.106.064311. [PMC free article] [PubMed] [Cross Ref]
  • O'Malley KG, Sakamoto T, Danzmann RG, Ferguson MM. Quantitative trait loci for spawning date and body weight in rainbow trout: testing for conserved effects across ancestrally duplicated chromosomes. J Hered. 2003;94:273–284. doi: 10.1093/jhered/esg067. [PubMed] [Cross Ref]
  • Perry GM, Danzmann RG, Ferguson MM, Gibson JP. Quantitative trait loci for upper thermal tolerance in outbred strains of rainbow trout (Oncorhynchus mykiss) Heredity. 2001;86:333–341. doi: 10.1046/j.1365-2540.2001.00838.x. [PubMed] [Cross Ref]
  • Perry GM, Ferguson MM, Sakamoto T, Danzmann RG. Sex-linked quantitative trait loci for thermotolerance and length in the rainbow trout. J Hered. 2005;96:97–107. doi: 10.1093/jhered/esi019. [PubMed] [Cross Ref]
  • Robison BD, Wheeler PA, Sundin K, Sikka P, Thorgaard GH. Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss) J Hered. 2001;92:16–22. doi: 10.1093/jhered/92.1.16. [PubMed] [Cross Ref]
  • Zimmerman AM, Evenhuis JP, Thorgaard GH, Ristow SS. A single major chromosomal region controls natural killer cell-like activity in rainbow trout. Immunogenetics. 2004;55:825–835. doi: 10.1007/s00251-004-0645-6. [PubMed] [Cross Ref]
  • Ozaki A, Sakamoto T, Khoo S, Nakamura K, Coimbra MR, Akutsu T, Okamoto N. Quantitative trait loci (QTLs) associated with resistance/susceptibility to infectious pancreatic necrosis virus (IPNV) in rainbow trout (Oncorhynchus mykiss) Mol Genet Genomics. 2001;265:23–31. doi: 10.1007/s004380000392. [PubMed] [Cross Ref]
  • Nakamura K, Ozaki A, Akutsu T, Iwai K, Sakamoto T, Yoshizaki G, Okamoto N. Genetic mapping of the dominant albino locus in rainbow trout (Oncorhynchus mykiss) Mol Genet Genomics. 2001;265:687–693. doi: 10.1007/s004380100464. [PubMed] [Cross Ref]
  • Young WP, Wheeler PA, Coryell VH, Keim P, Thorgaard GH. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics. 1998;148:839–850. [PMC free article] [PubMed]
  • Nichols KM, Young WP, Danzmann RG, Robison BD, Rexroad CE, 3rd, Noakes M, Phillips RB, Bentzen P, Spies I, Knudsen K, et al. A consolidated linkage map for rainbow trout (Oncorhynchus mykiss) Anim Genet. 2003;34:102–115. doi: 10.1046/j.1365-2052.2003.00957.x. [PubMed] [Cross Ref]
  • Guyomard R, Mauger S, Tabet-Canale K, Martineau S, Genet C, Krieg F, Quillet E. A type I and type II microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) with presumptive coverage of all chromosome arms. BMC Genomics. 2006;7:302. doi: 10.1186/1471-2164-7-302. [PMC free article] [PubMed] [Cross Ref]
  • Phillips RB, Nichols KM, DeKoning JJ, Morasch MR, Keatley KA, Rexroad C, 3rd, Gahr SA, Danzmann RG, Drew RE, Thorgaard GH. Assignment of rainbow trout linkage groups to specific chromosomes. Genetics. 2006;174:1661–1670. doi: 10.1534/genetics.105.055269. [PMC free article] [PubMed] [Cross Ref]
  • Nichols KM, Young WP, Danzmann RG, Robison BD, Rexroad C, Noakes M, Phillips RB, Bentzen P, Spies I, Knudsen K, et al. A consolidated linkage map for rainbow trout (Oncorhynchus mykiss) Anim Genet. 2003;34:102–115. doi: 10.1046/j.1365-2052.2003.00957.x. [PubMed] [Cross Ref]
  • Danzmann RG, Cairney M, Davidson WS, Ferguson MM, Gharbi K, Guyomard R, Holm LE, Leder E, Okamoto N, Ozaki A, et al. A comparative analysis of the rainbow trout genome with 2 other species of fish (Arctic charr and Atlantic salmon) within the tetraploid derivative Salmonidae family (subfamily: Salmoninae) Genome. 2005;48:1037–1051. doi: 10.1139/g05-067. [PubMed] [Cross Ref]
  • Liu ZJ, Cordes JF. DNA marker technologies and their applications in aquaculture genetics. Aquaculture. 2004;238:1–37. doi: 10.1016/j.aquaculture.2004.05.027. [Cross Ref]
  • Womack JE, Kata SR. Bovine genome mapping: evolutionary inference and the power of comparative genomics. Curr Opin Genet Dev. 1995;5:725–733. doi: 10.1016/0959-437X(95)80004-O. [PubMed] [Cross Ref]
  • Collins FS. Positional cloning moves from perditional to traditional. Nat Genet. 1995;9:347–350. doi: 10.1038/ng0495-347. [PubMed] [Cross Ref]
  • Chistiakov DA, Hellemans B, Volckaert FAM. Microsatellites and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics. Aquaculture. 2006;255:1–29. doi: 10.1016/j.aquaculture.2005.11.031. [Cross Ref]
  • Holm LE. Omy0002DIAS: a highly polymorphic dinucleotide microsatellite in rainbow trout (Oncorhynchus mykiss) Anim Genet. 1998;29:465. [PubMed]
  • Ozaki A, Khoo SK, Sakamoto T, Okamoto N. Two highly polymorphic dinucleotide microsatellites in rainbow trout (Oncorhynchus mykiss): OmyRGT7TUF and OmyRGT8TUF. Anim Genet. 1999;30:393–394. doi: 10.1046/j.1365-2052.1999.00526-12.x. [PubMed] [Cross Ref]
  • Khoo SK, Ozaki A, Sakamoto T, Okamoto N. Rapid communication: two highly polymorphic dinucleotide microsatellites in rainbow trout (Oncorhynchus mykiss): OmyRGT18TUF and OmyRGT23TUF. J Anim Sci. 2000;78:490–491. [PubMed]
  • Khoo SK, Ozaki A, Sakamoto T, Okamoto N. Four highly polymorphic dinucleotide microsatellites in rainbow trout (Oncorhynchus mykiss) Anim Genet. 2000;31:73–74. doi: 10.1111/j.1365-2052.2000.579-8.x. [PubMed] [Cross Ref]
  • Rexroad CE, 3rd, Coleman RL, Martin AM, Hershberger WK, Killefer J. Thirty-five polymorphic microsatellite markers for rainbow trout (Oncorhynchus mykiss) Anim Genet. 2001;32:317–319. doi: 10.1046/j.1365-2052.2001.0730b.x. [PubMed] [Cross Ref]
  • Palti Y, Fincham MR, Rexroad CE., 3rd Characterization of 38 polymorphic microsatellite markers for rainbow trout (Oncorhynchus mykiss) Molecular Ecology Notes. 2002;2:449–452. doi: 10.1046/j.1471-8286.2002.00274.x. [Cross Ref]
  • Rexroad CE, 3rd, Coleman RL, Gustafson AL, Hershberger WK, Killefer J. Development of rainbow trout microsatellite markers from repeat enriched libraries. Mar Biotechnol (NY) 2002;4:12–16. doi: 10.1007/s10126-001-0058-6. [PubMed] [Cross Ref]
  • Rexroad CE, 3rd, Coleman RL, Hershberger WK, Killefer J. Rapid communication: Thirty-eight polymorphic microsatellite markers for mapping in rainbow trout. J Anim Sci. 2002;80:541–542. [PubMed]
  • Rexroad CE, Coleman RL, Hershberger WK, Killefer J. Eighteen polymorphic microsatellite markers for rainbow trout (Oncorhynchus mykiss) Anim Genet. 2002;33:76–78. doi: 10.1046/j.1365-2052.2002.0742d.x. [PubMed] [Cross Ref]
  • Ozaki A, Khoo SK, Sakamoto T, Okamoto N. Thirty-one polymorphic microsatellite markers for genetic mapping in rainbow trout (Oncorhynchus mykiss) Anim Genet. 2003;34:315–317. doi: 10.1046/j.1365-2052.2003.01031.x. [PubMed] [Cross Ref]
  • Palti Y, Danzmann RG, Rexroad CE., 3rd Characterization and mapping of 19 polymorphic microsatellite markers for rainbow trout (Oncorhynchus mykiss) Anim Genet. 2003;34:153–156. doi: 10.1046/j.1365-2052.2003.00965_7.x. [PubMed] [Cross Ref]
  • Rexroad CE, 3rd, Palti Y. Development of Ninety-Seven Polymorphic Microsatellite Markers for rainbow trout (Oncorhynchus mykiss) Transactions of the American Fisheries Society. 2003;132:1214–1221. doi: 10.1577/T02-086. [Cross Ref]
  • Rodriguez FM, Rexroad CE, 3rd, Palti Y. Characterization of twenty-four microsatellite markers for rainbow trout (Oncorhynchus mykiss) Molecular Ecology Notes. 2003;3:619–622. doi: 10.1046/j.1471-8286.2003.00531.x. [Cross Ref]
  • Coulibaly I, Gharbi K, Danzmann RG, Yao J, Rexroad CE., 3rd Characterization and comparison of microsatellites derived from repeat-enriched libraries and expressed sequence tags. Anim Genet. 2005;36:309–315. doi: 10.1111/j.1365-2052.2005.01305.x. [PubMed] [Cross Ref]
  • Gilbey J, Verspoor E, McLay A, Houlihan D. A microsatellite linkage map for Atlantic salmon (Salmo salar) Anim Genet. 2004;35:98–105. doi: 10.1111/j.1365-2052.2004.01091.x. [PubMed] [Cross Ref]
  • Gharbi K, Gautier A, Danzmann RG, Gharbi S, Sakamoto T, Hoyheim B, Taggart JB, Cairney M, Powell R, Krieg F, et al. A linkage map for brown trout (Salmo trutta): chromosome homeologies and comparative genome organization with other salmonid fish. Genetics. 2006;172:2405–2419. doi: 10.1534/genetics.105.048330. [PMC free article] [PubMed] [Cross Ref]
  • Moen T, Hoyheim B, Munck H, Gomez-Raya L. A linkage map of Atlantic salmon (Salmo salar) reveals an uncommonly large difference in recombination rate between the sexes. Anim Genet. 2004;35:81–92. doi: 10.1111/j.1365-2052.2004.01097.x. [PubMed] [Cross Ref]
  • Rodriguez MF, Gahr SA, Rexroad CE, 3rd, Palti Y. Rapid microsatellite detection from rainbow trout (Oncorhynchus mykiss) bacterial artificial chromosome using PCR screening method. Marine Biotechnology. 2006;8:346–350. doi: 10.1007/s10126-005-5064-7. [PubMed] [Cross Ref]
  • Rexroad CE, 3rd, Rodriguez MF, Coulibaly I, Gharbi K, Danzmann RG, Dekoning J, Phillips R, Palti Y. Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout (Oncorhynchus mykiss) BMC Genomics. 2005;6:54. doi: 10.1186/1471-2164-6-54. [PMC free article] [PubMed] [Cross Ref]
  • Davisson MT, Wright JE, Atherton LM. Cytogenetic Analysis of Pseudolinkage of Ldh Loci in the Teleost Genus Salvelinus. Genetics. 1973;73:645–658. [PMC free article] [PubMed]
  • Voorrips RE. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered. 2002;93:77–78. doi: 10.1093/jhered/93.1.77. [PubMed] [Cross Ref]
  • Rainbow Trout Unigene http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=8022
  • Rainbow Trout Gene Index
  • Young WP, Wheeler PA, Fields RD, Thorgaard GH. DNA fingerprinting confirms isogenicity of androgenetically derived rainbow trout lines. J Hered. 1996;87:77–80. [PubMed]
  • Phillips RB, Nichols KM, DeKoning JJ, Morash MR, Keatley KA, Rexroad CE, 3rd, Danzmann RG, Drew RE, Thorgaard GH. Assignment of Rainbow Trout Linkage Groups to Specific Chromosomes. Genetics. 2006;174:1661–1670. doi: 10.1534/genetics.105.055269. [PMC free article] [PubMed] [Cross Ref]
  • Silverstein JT, King T, Rexroad CE., 3rd Genetic Variation Measured by Microsatellites Among Three Strains of Domesticated Rainbow Trout. Aquaculture Research. 2004;35:40–48. doi: 10.1111/j.1365-2109.2004.00979.x. [Cross Ref]
  • Sambrook J, Russell D. Molecular cloning : a laboratory manual. 3. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press; 2001.
  • Coulibaly I, Danzmann RG, Palti Y, Vallejo R, Gahr SA, Yao J, Rexroad CE. Mapping of genes in a region associated with upper temperature tolerance in rainbow trout. Anim Genet. 2006;37:598–599. doi: 10.1111/j.1365-2052.2006.01529.x. [PubMed] [Cross Ref]
  • Palti Y, Rodriguez MF, Vallejo R, III CER. Mapping of toll-like receptor (TLR) genes in rainbow trout. Animal Genetics. 2006;37:597–598. doi: 10.1111/j.1365-2052.2006.01527.x. [PubMed] [Cross Ref]
  • Johnson N, Vallejo R, Silverstein J, Welch T, Wiens G, Hallerman E, Palti Y. Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss) Mar Biotechnol (NY) 2008;10:429–437. doi: 10.1007/s10126-007-9080-7. [PubMed] [Cross Ref]
  • Boutin-Ganache I, Raposo M, Raymond M, Deschepper CF. M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. Biotechniques. 2001;31:24–26, 28. [PubMed]
  • Lathrop GM, Lalouel JM, Julier C, Ott J. Strategies for multilocus linkage analysis in humans. Proc Natl Acad Sci USA. 1984;81:3443–3446. doi: 10.1073/pnas.81.11.3443. [PMC free article] [PubMed] [Cross Ref]
  • O'Connell JR, Weeks DE. PedCheck: a program for identification of genotype incompatibilities in linkage analysis. Am J Hum Genet. 1998;63:259–266. doi: 10.1086/301904. [PMC free article] [PubMed] [Cross Ref]
  • Weeks DE. RECODE. 1995.
  • Kashuk C, Chakravarti A, Matise T. Documentation for LNKTOCRI++ 1997
  • Lander ES, Green P. Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci USA. 1987;84:2363–2367. doi: 10.1073/pnas.84.8.2363. [PMC free article] [PubMed] [Cross Ref]
  • Matise TC, Perlin M, Chakravarti A. Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage map. Nat Genet. 1994;6:384–390. doi: 10.1038/ng0494-384. [PubMed] [Cross Ref]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–410. [PubMed]

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