• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Nov 1988; 56(11): 2891–2895.
PMCID: PMC259667

Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae.


El Tor and non-O1 strains of Vibrio cholerae were analyzed to determine whether synthesis of secreted hemolysin was influenced by the concentration of iron in the medium. Synthesis of hemolysin was found to be iron regulated in both El Tor and non-O1 isolates. Increased levels of hemolytic activity were detected in supernatants of iron-starved cells. Spontaneous hemolysin-deficient mutants of one non-O1 strain were found to occur at high frequency. These variants also failed to synthesize vibriobactin, the iron transport compound utilized by V. cholerae. Another non-O1 strain was found to synthesize both hemolysin and vibriobactin constitutively. When the cloned Escherichia coli fur gene, encoded on the plasmid pABN203, was introduced into this constitutive strain, normal iron regulation of both hemolysin and vibriobactin was reestablished. The ability of V. cholerae to utilize mammalian iron compounds was determined, and it was found that both hemin and hemoglobin could serve as sole sources of iron.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bagg A, Neilands JB. Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiol Rev. 1987 Dec;51(4):509–518. [PMC free article] [PubMed]
  • Bullen JJ. The significance of iron in infection. Rev Infect Dis. 1981 Nov-Dec;3(6):1127–1138. [PubMed]
  • Goldberg SL, Murphy JR. Molecular cloning of the hemolysin determinant from Vibrio cholerae El Tor. J Bacteriol. 1984 Oct;160(1):239–244. [PMC free article] [PubMed]
  • Goldberg SL, Murphy JR. Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B. J Bacteriol. 1985 Apr;162(1):35–41. [PMC free article] [PubMed]
  • Griffiths GL, Sigel SP, Payne SM, Neilands JB. Vibriobactin, a siderophore from Vibrio cholerae. J Biol Chem. 1984 Jan 10;259(1):383–385. [PubMed]
  • Grünig HM, Rutschi D, Schoch C, Lebek G. The chromosomal fur gene regulates the extracellular haemolytic activity encoded by certain hly plasmids. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Aug;266(1-2):231–238. [PubMed]
  • Honda T, Finkelstein RA. Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios. Infect Immun. 1979 Dec;26(3):1020–1027. [PMC free article] [PubMed]
  • Konopka K, Bindereif A, Neilands JB. Aerobactin-mediated utilization of transferrin iron. Biochemistry. 1982 Dec 7;21(25):6503–6508. [PubMed]
  • Lebek G, Gruenig HM. Relation between the hemolytic property and iron metabolism in Escherichia coli. Infect Immun. 1985 Dec;50(3):682–686. [PMC free article] [PubMed]
  • Linggood MA, Ingram PL. The role of alpha haemolysin in the virulence of Escherichia coli for mice. J Med Microbiol. 1982 Feb;15(1):23–30. [PubMed]
  • McIntyre OR, Feeley JC. Characteristics of non-cholera Vibrios isolated from cases of human diarrhoea. Bull World Health Organ. 1965;32(5):627–632. [PMC free article] [PubMed]
  • Mercurio A, Manning PA. Cellular localization and export of the soluble haemolysin of Vibrio cholerae El Tor. Mol Gen Genet. 1985;200(3):472–475. [PubMed]
  • Neilands JB. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. [PubMed]
  • Payne SM, Finkelstein RA. The critical role of iron in host-bacterial interactions. J Clin Invest. 1978 Jun;61(6):1428–1440. [PMC free article] [PubMed]
  • Payne SM, Finkelstein RA. Siderophore production by Vibrio cholerae. Infect Immun. 1978 Apr;20(1):310–311. [PMC free article] [PubMed]
  • Richardson K, Michalski J, Kaper JB. Hemolysin production and cloning of two hemolysin determinants from classical Vibrio cholerae. Infect Immun. 1986 Nov;54(2):415–420. [PMC free article] [PubMed]
  • Rogers HJ. Iron-Binding Catechols and Virulence in Escherichia coli. Infect Immun. 1973 Mar;7(3):445–456. [PMC free article] [PubMed]
  • Sciortino CV, Finkelstein RA. Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo. Infect Immun. 1983 Dec;42(3):990–996. [PMC free article] [PubMed]
  • Sigel SP, Lanier S, Baselski VS, Parker CD. In vivo evaluation of pathogenicity of clinical and environmental isolates of Vibrio cholerae. Infect Immun. 1980 Jun;28(3):681–687. [PMC free article] [PubMed]
  • Sigel SP, Payne SM. Effect of iron limitation on growth, siderophore production, and expression of outer membrane proteins of Vibrio cholerae. J Bacteriol. 1982 Apr;150(1):148–155. [PMC free article] [PubMed]
  • Sigel SP, Stoebner JA, Payne SM. Iron-vibriobactin transport system is not required for virulence of Vibrio cholerae. Infect Immun. 1985 Feb;47(2):360–362. [PMC free article] [PubMed]
  • SIMON EH, TESSMAN I. THYMIDINE-REQUIRING MUTANTS OF PHAGE T4. Proc Natl Acad Sci U S A. 1963 Sep;50:526–532. [PMC free article] [PubMed]
  • Waalwijk C, MacLaren DM, de Graaff J. In vivo function of hemolysin in the nephropathogenicity of Escherichia coli. Infect Immun. 1983 Oct;42(1):245–249. [PMC free article] [PubMed]
  • Weinberg ED. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. [PMC free article] [PubMed]
  • Yamamoto K, Al-Omani M, Honda T, Takeda Y, Miwatani T. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin. Infect Immun. 1984 Jul;45(1):192–196. [PMC free article] [PubMed]
  • Yamamoto K, Ichinose Y, Nakasone N, Tanabe M, Nagahama M, Sakurai J, Iwanaga M. Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor. Infect Immun. 1986 Mar;51(3):927–931. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...