• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Aug 1988; 56(8): 1897–1900.
PMCID: PMC259498

Two distinct forms of Chlamydia psittaci associated with disease and infertility in Phascolarctos cinereus (koala).

Abstract

While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strains of C. psittaci infect koalas; one strain was isolated from the conjunctivae, and the other was isolated from the urogenital tract and the rectum. A gene probe, pFEN207, containing the coding sequence for an enzyme involved in the biosynthesis of the chlamydial genus-specific lipopolysaccharide antigen, and a separate probe, pCPML-4N, prepared from a DNA fragment of a koala-infecting strain of C. psittaci, were used to determine the patterns of hybridization in the koala-infecting strains; these patterns were found to be quite distinct from those observed with C. psittaci isolates from other animals. We also demonstrated by hybridization analysis with an avian strain plasmid that all three koala urogenital isolates contain a plasmid and that there is no evidence for the presence of a homologous plasmid in any of the ocular isolates.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cockram FA, Jackson AR. Letter: Isolation of a Chlamydia from cases of keratoconjunctivitis in koalas. Aust Vet J. 1974 Feb;50(2):82–83. [PubMed]
  • Horn JE, Hammer ML, Falkow S, Quinn TC. Detection of Chlamydia trachomatis in tissue culture and cervical scrapings by in situ DNA hybridization. J Infect Dis. 1986 Jun;153(6):1155–1159. [PubMed]
  • Joseph T, Nano FE, Garon CF, Caldwell HD. Molecular characterization of Chlamydia trachomatis and Chlamydia psittaci plasmids. Infect Immun. 1986 Feb;51(2):699–703. [PMC free article] [PubMed]
  • Kingsbury DT, Weiss E. Lack of deoxyribonucleic acid homology between species of the genus Chlamydia. J Bacteriol. 1968 Oct;96(4):1421–1423. [PMC free article] [PubMed]
  • McClenaghan M, Herring AJ, Aitken ID. Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect Immun. 1984 Aug;45(2):384–389. [PMC free article] [PubMed]
  • McColl KA, Martin RW, Gleeson LJ, Handasyde KA, Lee AK. Chlamydia infection and infertility in the female koala (Phascolarctos cinereus). Vet Rec. 1984 Dec 22;115(25-26):655–655. [PubMed]
  • McKenzie RA. Observations on diseases of free-living and captive koalas. Aust Vet J. 1981 May;57(5):243–246. [PubMed]
  • Nano FE, Caldwell HD. Expression of the chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science. 1985 May 10;228(4700):742–744. [PubMed]
  • Nilsson B, Uhlén M, Josephson S, Gatenbeck S, Philipson L. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 1983 Nov 25;11(22):8019–8030. [PMC free article] [PubMed]
  • Obendorf DL. Pathology of the female reproductive tract in the koala, Phascolarctos cinereus (Goldfuss), from Victoria, Australia. J Wildl Dis. 1981 Oct;17(4):587–592. [PubMed]
  • Obendorf DL. Causes of mortality and morbidity of wild koalas, Phascolarctos cinereus (Goldfuss), in Victoria, Australia. J Wildl Dis. 1983 Apr;19(2):123–131. [PubMed]
  • Palmer L, Falkow S. A common plasmid of Chlamydia trachomatis. Plasmid. 1986 Jul;16(1):52–62. [PubMed]
  • Peterson EM, de la Maza LM. Characterization of Chlamydia DNA by restriction endonuclease cleavage. Infect Immun. 1983 Aug;41(2):604–608. [PMC free article] [PubMed]
  • Schachter J, Caldwell HD. Chlamydiae. Annu Rev Microbiol. 1980;34:285–309. [PubMed]
  • Storz J, Spears P. Chlamydiales: properties, cycle of development and effect on eukaryotic host cells. Curr Top Microbiol Immunol. 1977;76:167–214. [PubMed]
  • Timms P, Eaves FW, Girjes AA, Lavin MF. Comparison of Chlamydia psittaci isolates by restriction endonuclease and DNA probe analyses. Infect Immun. 1988 Jan;56(1):287–290. [PMC free article] [PubMed]
  • Wenman WM, Lovett MA. Expression in E. coli of Chlamydia trachomatis antigen recognized during human infection. Nature. 1982 Mar 4;296(5852):68–70. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...