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FIGURE 2.

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Plaque assays of recovered viruses from parental (WT+G) and 3′-deletion mutants (Δ4+GC and Δ10+GGC) input RNAs. Viruses were collected from supernatants on the indicated days and plaque titers were determined. The plaque assays were repeated three times and the plaque titers (pfu/mL) of a representative experiment are given. The average plaque sizes (mm) at indicated days are as follows. WT: day 20: 8.0 × 103, 2.64 ± 1.06 mm. Δ4+GC RNA: day 13: 1.2 × 104, 1.25 ± 0.44 mm; day 20: 1.1 × 104, 1.86 ± 0.57 mm; day 27: 7.5 × 104, 2.41 ± 1.18 mm; day 48: 1.5 × 104, 2.67 ± 0.81 mm. Δ10+GGC: day 48: 4.0 × 103, 1.52 ± 0.51 mm; day 62: 8.5 × 103, 2.31 ± 0.83 mm.

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