• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of yjbmLink to Publisher's site
Yale J Biol Med. 1983 Sep-Dec; 56(5-6): 723–727.
PMCID: PMC2590514

A plasmid from S. citri strain M14 hybridizes with extrachromosomal DNAs from other spiroplasmas, including corn stunt spiroplasma E275, tick spiroplasma 277F, and coco spiroplasma N525.

Abstract

A plasmid, pM41, has been isolated from the Spiroplasma citri strain M4 (group I-1) and characterized by restriction mapping. Using a 32P-labeled probe specific of the plasmid, we have shown by DNA-DNA hybridization that a plasmid identical to pM41 or a closely related plasmid, is present in several, but not all, S. citri strains. DNA sequences that hybridize to pM41 were also identified in three other spiroplasmas not belonging to the S. citri species. Protein patterns of several S. citri strains have been compared in order to investigate the effect of pM41 on the spiroplasma protein profiles or maps. In fact, the presence of pM41 does not appear to modify the protein pattern.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ranhand JM, Mitchell WO, Popkin TJ, Cole RM. Covalently closed circular deoxyribonucleic acids in spiroplasmas. J Bacteriol. 1980 Sep;143(3):1194–1199. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Botchan M, Topp W, Sambrook J. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell. 1976 Oct;9(2):269–287. [PubMed]
  • Bové JM, Mouches C, Carle-Junca P, Degorce-Dumas JR, Tully JG, Whitcomb RF. Spiroplasmas of Group I: the Spiroplasma citri cluster. Yale J Biol Med. 1983 Sep-Dec;56(5-6):573–582. [PMC free article] [PubMed]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...