• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Jun 1990; 58(6): 1769–1773.
PMCID: PMC258721

Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus.


Translucent, avirulent spontaneous phase variants of Vibrio vulnificus MO6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. Two translucent, acapsular mutants, which did not show phase variation, were constructed by using the transposon Tn5 IS50L::phoA (TnphoA). Loss of capsule was accompanied by decreases in virulence, hydrophilicity, and serum resistance. The ability to utilize transferrin-bound iron for growth was lost in only one of the two unencapsulated mutants. Our data emphasize the apparent importance of capsule in the virulence of V. vulnificus and indicate that utilization of transferrin-bound iron is independent of encapsulation.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allen PM, Roberts I, Boulnois GJ, Saunders JR, Hart CA. Contribution of capsular polysaccharide and surface properties to virulence of Escherichia coli K1. Infect Immun. 1987 Nov;55(11):2662–2668. [PMC free article] [PubMed]
  • Blake PA, Merson MH, Weaver RE, Hollis DG, Heublein PC. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. [PubMed]
  • Blaser MJ, Smith PF, Repine JE, Joiner KA. Pathogenesis of Campylobacter fetus infections. Failure of encapsulated Campylobacter fetus to bind C3b explains serum and phagocytosis resistance. J Clin Invest. 1988 May;81(5):1434–1444. [PMC free article] [PubMed]
  • Bonner JR, Coker AS, Berryman CR, Pollock HM. Spectrum of Vibrio infections in a Gulf Coast community. Ann Intern Med. 1983 Oct;99(4):464–469. [PubMed]
  • Carruthers MM, Kabat WJ. Vibrio vulnificus (lactose-positive vibrio) and Vibrio parahaemolyticus differ in their susceptibilities to human serum. Infect Immun. 1981 May;32(2):964–966. [PMC free article] [PubMed]
  • Engebrecht J, Simon M, Silverman M. Measuring gene expression with light. Science. 1985 Mar 15;227(4692):1345–1347. [PubMed]
  • Schryvers AB, Gonzalez GC. Comparison of the abilities of different protein sources of iron to enhance Neisseria meningitidis infection in mice. Infect Immun. 1989 Aug;57(8):2425–2429. [PMC free article] [PubMed]
  • Klegerman ME, Boyer KM, Papierniak CK, Levine L, Gotoff SP. Type-specific capsular antigen is associated with virulence in late-onset group B Streptococcal type III disease. Infect Immun. 1984 Apr;44(1):124–129. [PMC free article] [PubMed]
  • Klontz KC, Lieb S, Schreiber M, Janowski HT, Baldy LM, Gunn RA. Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987. Ann Intern Med. 1988 Aug 15;109(4):318–323. [PubMed]
  • Kreger A, DeChatelet L, Shirley P. Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J Infect Dis. 1981 Sep;144(3):244–248. [PubMed]
  • Kreger AS, Gray LD, Testa J. Protection of mice against Vibrio vulnificus disease by vaccination with surface antigen preparations and anti-surface antigen antisera. Infect Immun. 1984 Sep;45(3):537–543. [PMC free article] [PubMed]
  • Luft JH. Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971 Nov;171(3):347–368. [PubMed]
  • Manoil C, Beckwith J. TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129–8133. [PMC free article] [PubMed]
  • Masson L, Holbein BE. Influence of nutrient limitation and low pH on serogroup B Neisseria meningitidis capsular polysaccharide levels: correlation with virulence for mice. Infect Immun. 1985 Feb;47(2):465–471. [PMC free article] [PubMed]
  • Miller JF, Mekalanos JJ, Falkow S. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science. 1989 Feb 17;243(4893):916–922. [PubMed]
  • Morris JG., Jr Vibrio vulnificus--a new monster of the deep? Ann Intern Med. 1988 Aug 15;109(4):261–263. [PubMed]
  • Morris JG, Jr, Black RE. Cholera and other vibrioses in the United States. N Engl J Med. 1985 Feb 7;312(6):343–350. [PubMed]
  • Simpson LM, White VK, Zane SF, Oliver JD. Correlation between virulence and colony morphology in Vibrio vulnificus. Infect Immun. 1987 Jan;55(1):269–272. [PMC free article] [PubMed]
  • Tacket CO, Brenner F, Blake PA. Clinical features and an epidemiological study of Vibrio vulnificus infections. J Infect Dis. 1984 Apr;149(4):558–561. [PubMed]
  • Taylor RK, Manoil C, Mekalanos JJ. Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae. J Bacteriol. 1989 Apr;171(4):1870–1878. [PMC free article] [PubMed]
  • Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene. 1984 Dec;32(3):369–379. [PubMed]
  • Wright AC, Simpson LM, Oliver JD. Role of iron in the pathogenesis of Vibrio vulnificus infections. Infect Immun. 1981 Nov;34(2):503–507. [PMC free article] [PubMed]
  • Yoshida S, Ogawa M, Mizuguchi Y. Relation of capsular materials and colony opacity to virulence of Vibrio vulnificus. Infect Immun. 1985 Feb;47(2):446–451. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...