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Infect Immun. Apr 1990; 58(4): 1120–1123.
PMCID: PMC258592

Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection.

Abstract

Proteus mirabilis, a urease-producing uropathogen, causes serious urinary tract infections in humans. To specifically evaluate the contribution of urease to virulence, a mutation was introduced into P. mirabilis HI4320 by homologous recombination. Virulence was assessed in the CBA mouse model of ascending urinary tract infection. Twenty mice each were challenged transurethrally with P. mirabilis HI4320 and its urease-negative derivative (1 x 10(9) to 2 x 10(9) CFU). At 48 h animals were sacrificed and the mean log10 CFU per milliliter of urine (parent, 6.23; mutant, 4.19; P = 0.0014) or per gram of bladder (parent, 6.29; mutant, 4.28; P = 0.0002), left kidney (parent, 4.11; mutant, 1.02; P = 0.00009), and right kidney (parent, 4.11; mutant, 2.43; P = 0.036) were all shown to be significantly different. These data demonstrate a role for urease as a critical virulence determinant for uropathogenic P. mirabilis.

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Selected References

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  • Aronson M, Medalia O, Griffel B. Prevention of ascending pyelonephritis in mice by urease inhibitors. Nephron. 1974;12(2):94–104. [PubMed]
  • BRAUDE AI, SHAPIRO AP, SIEMIENSKI J. Hematogenous pyelonephritis in rats. I. Its pathogenesis when produced by a simple new method. J Clin Invest. 1955 Oct;34(10):1489–1497. [PMC free article] [PubMed]
  • BRAUDE AI, SIEMIENSKI J. Role of bacterial urease in experimental pyelonephritis. J Bacteriol. 1960 Aug;80:171–179. [PMC free article] [PubMed]
  • Fairley KF, Carson NE, Gutch RC, Leighton P, Grounds AD, Laird EC, McCallum PH, Sleeman RL, O'Keefe CM. Site of infection in acute urinary-tract infection in general practice. Lancet. 1971 Sep 18;2(7725):615–618. [PubMed]
  • Fitzpatrick FK. Pyelonephritis in the mouse. I. Infection experiments. Proc Soc Exp Biol Med. 1966 Nov;123(2):336–339. [PubMed]
  • Gatermann S, Marre R. Cloning and expression of Staphylococcus saprophyticus urease gene sequences in Staphylococcus carnosus and contribution of the enzyme to virulence. Infect Immun. 1989 Oct;57(10):2998–3002. [PMC free article] [PubMed]
  • GORRILL RH. The effect of obstruction of the ureter on the renal localisation of bacteria. J Pathol Bacteriol. 1956 Jul;72(1):59–71. [PubMed]
  • GORRILL RH. THE FATE OF PSEUDOMONAS AERUGINOSA, PROTEUS MIRABILIS AND ESCHERICHIA COLI IN THE MOUSE KIDNEY. J Pathol Bacteriol. 1965 Jan;89:81–88. [PubMed]
  • Griffith DP, Musher DM, Itin C. Urease. The primary cause of infection-induced urinary stones. Invest Urol. 1976 Mar;13(5):346–350. [PubMed]
  • Hagberg L, Engberg I, Freter R, Lam J, Olling S, Svanborg Edén C. Ascending, unobstructed urinary tract infection in mice caused by pyelonephritogenic Escherichia coli of human origin. Infect Immun. 1983 Apr;40(1):273–283. [PMC free article] [PubMed]
  • Johnson DE, Lockatell CV, Hall-Craigs M, Mobley HL, Warren JW. Uropathogenicity in rats and mice of Providencia stuartii from long-term catheterized patients. J Urol. 1987 Sep;138(3):632–635. [PubMed]
  • Johnson ET. The condom catheter: urinary tract infection and other complications. South Med J. 1983 May;76(5):579–582. [PubMed]
  • Jones BD, Mobley HL. Proteus mirabilis urease: genetic organization, regulation, and expression of structural genes. J Bacteriol. 1988 Aug;170(8):3342–3349. [PMC free article] [PubMed]
  • Jones BD, Mobley HL. Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease. J Bacteriol. 1989 Dec;171(12):6414–6422. [PMC free article] [PubMed]
  • Kuntze JR, Weinberg AC, Ahlering TE. Hyperammonemic coma due to Proteus infection. J Urol. 1985 Nov;134(5):972–973. [PubMed]
  • MacLaren DM. The significance of urease in proteus pyelonephritis: a bacteriological study. J Pathol Bacteriol. 1968 Jul;96(1):45–56. [PubMed]
  • MacLaren DM. The significance of urease in proteus pyelonephritis: a histological and biochemical study. J Pathol. 1969 Jan;97(1):43–49. [PubMed]
  • Maclaren DM. The influence of acetohydroxamic acid on experimental proteus pyelonephritis. Invest Urol. 1974 Sep;12(2):146–149. [PubMed]
  • Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol. 1988 Jun;170(6):2575–2583. [PMC free article] [PubMed]
  • Mobley HL, Warren JW. Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol. 1987 Nov;25(11):2216–2217. [PMC free article] [PubMed]
  • Musher DM, Griffith DP, Yawn D, Rossen RD. Role of urease in pyelonephritis resulting from urinary tract infection with Proteus. J Infect Dis. 1975 Feb;131(2):177–181. [PubMed]
  • Old DC, Adegbola RA. Haemagglutinins and fimbriae of Morganella, Proteus and Providencia. J Med Microbiol. 1982 Nov;15(4):551–564. [PubMed]
  • Warren JW, Damron D, Tenney JH, Hoopes JM, Deforge B, Muncie HL., Jr Fever, bacteremia, and death as complications of bacteriuria in women with long-term urethral catheters. J Infect Dis. 1987 Jun;155(6):1151–1158. [PubMed]
  • Williams JJ, Rodman JS, Peterson CM. A randomized double-blind study of acetohydroxamic acid in struvite nephrolithiasis. N Engl J Med. 1984 Sep 20;311(12):760–764. [PubMed]

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