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PLoS Pathog. Nov 2008; 4(11): e1000217.
Published online Nov 21, 2008. doi:  10.1371/journal.ppat.1000217
PMCID: PMC2581891

The Hyphal-Associated Adhesin and Invasin Als3 of Candida albicans Mediates Iron Acquisition from Host Ferritin

Aaron P. Mitchell, Editor

Abstract

Iron sequestration by host iron-binding proteins is an important mechanism of resistance to microbial infections. Inside oral epithelial cells, iron is stored within ferritin, and is therefore not usually accessible to pathogenic microbes. We observed that the ferritin concentration within oral epithelial cells was directly related to their susceptibility to damage by the human pathogenic fungus, Candida albicans. Thus, we hypothesized that host ferritin is used as an iron source by this organism. We found that C. albicans was able to grow on agar at physiological pH with ferritin as the sole source of iron, while the baker's yeast Saccharomyces cerevisiae could not. A screen of C. albicans mutants lacking components of each of the three known iron acquisition systems revealed that only the reductive pathway is involved in iron utilization from ferritin by this fungus. Additionally, C. albicans hyphae, but not yeast cells, bound ferritin, and this binding was crucial for iron acquisition from ferritin. Transcriptional profiling of wild-type and hyphal-defective C. albicans strains suggested that the C. albicans invasin-like protein Als3 is required for ferritin binding. Hyphae of an Δals3 null mutant had a strongly reduced ability to bind ferritin and these mutant cells grew poorly on agar plates with ferritin as the sole source of iron. Heterologous expression of Als3, but not Als1 or Als5, two closely related members of the Als protein family, allowed S. cerevisiae to bind ferritin. Immunocytochemical localization of ferritin in epithelial cells infected with C. albicans showed ferritin surrounding invading hyphae of the wild-type, but not the Δals3 mutant strain. This mutant was also unable to damage epithelial cells in vitro. Therefore, C. albicans can exploit iron from ferritin via morphology dependent binding through Als3, suggesting that this single protein has multiple virulence attributes.

Author Summary

Iron is an essential nutrient for all microbes. Many human pathogenic microbes have developed sophisticated strategies to acquire iron from the host as most compartments in the body contain little free iron. For example, in oral epithelial cells intracellular iron is bound to ferritin, a protein that is highly resistant to microbial attack. In fact, no microorganism has so far been shown to directly exploit ferritin as an iron source during interaction with host cells. This study demonstrates that the pathogenic fungus Candida albicans can use ferritin as the sole source of iron. Most intriguingly, C. albicans binds ferritin via a receptor that is only exposed on invasive hyphae. This receptor is Als3, which is a member of the Als-protein family. Als3 was previously demonstrated to be an adhesin with invasin-like properties. Mutants lacking Als3 failed to bind ferritin, grew poorly with ferritin as an iron source and were unable to damage epithelial cells. Strains of the baker's yeast expressing C. albicans Als3, but not two closely related proteins, Als1 or Als5, were able to bind ferritin. Therefore, C. albicans uses an additional morphology specific and unique iron uptake strategy based on ferritin while invading into host cells where ferritin is located.

Introduction

Iron is an essential element for virtually all organisms, ranging from microbes to multicellular animals. Higher organisms can sequester iron using high-affinity iron-binding molecules, so that it is unavailable to microorganisms. Iron sequestration provides a natural resistance to infections which has been described as “nutritional immunity” [1].

Successful microbial pathogens have developed multiple iron acquisition and uptake systems (reviewed in [2],[3]). These systems include enzymes for reduction and oxidization of iron ions (Fe2+ or Fe3+), high-affinity permeases for iron transport, chelators (siderophores) and uptake systems for siderophores. In the human body, the majority of iron is bound to iron-containing proteins with physiological functions (for example heme proteins such as hemoglobin), iron-binding transport proteins (transferrin), antimicrobial proteins (lactoferrin), or cellular iron storage proteins (ferritin). With the exception of ferritin, each of these proteins has been reported to serve as an iron source for some pathogenic microbes. These iron sources are exploited via direct binding, degradation, and/or uptake [4][13].

In mammalian cells, extracellular ferric iron is bound by apotransferrin (transferrin without iron). The binding of apotransferrin to two ferric iron molecules (holotransferrin, hTF) increases by two-fold its affinity for the transferrin receptor (TFR) present on the surface of virtually all mammalian cells. Following endocytosis of the hTF-TFR complex into the early endosome, acidification to low pH (pH 5.6) results in the release of iron from holotransferrin. The released ferrous iron is then transported to the cytoplasm by the divalent metal transporter (DMT1) and either used for cellular metabolism or stored within ferritin. The resulting apotransferrin is recycled to the cell surface and released at physiological pH (7.4) [14][16].

Ferritin is the main intracellular storage protein for iron (reviewed in [17]), containing approximately 30% of the total human body iron (66% is bound to hemoglobin). Ferritin consist of a 24-subunit protein shell of approximately 500 kDa. One ferritin molecule can contain up to 4,500 Fe3+ ions. The quaternary structure of ferritin is dissociated at acidic pH [18]. Its intracellular concentration can be increased by addition of exogenous iron and decreased by addition of an iron chelator [19]. Under iron-limiting conditions, cytosolic ferritin is autophagocytosed and subsequently degraded within acidic lysosomes [19],[20] and the iron becomes available to the cell. Outside of lysosomes, ferritin is an extremely robust and stable protein which seems to be resistant to all known microbial activities. In fact, the only microorganism that has so far been shown experimentally to exploit holoferritin as an iron source during interaction with host cells is Neisseria meningitidis [21]. N. meningitidis bacteria can trigger degradation of cytosolic ferritin within infected epithelial cells by manipulating the cellular machinery and lysosomal activity [22].

To secure sufficient iron availability whilst avoiding toxicity by iron mediated processes, a tight regulation of iron metabolism is essential. Some pathogenic microbes seem to have linked the availability of iron with expression of virulence attributes. For example, the expression of virulence genes in Listeria monocytogenes was found to be positively controlled by iron limitation [23] and infections with Mycobacterium tuberculosis were reported to be more fatal when iron was accessible [24]. In pathogenic Escherichia coli strains, more than 90 genes involved in iron acquisition and several other cellular functions such as chemotaxis, respiration, DNA synthesis, glycolysis and the tricarboxylic acid cycle are co-regulated by the global iron-dependent regulator FUR (Ferric-Uptake Regulator protein) [2]. The fungus, Cryptococcus neoformans has recently been shown to co-regulate iron uptake mechanisms with two key virulence properties, capsule formation and melanin production [25],[26]. Such coordinated regulation indicates that sensing the low iron content of the host environment is a key signal for pathogenic microbes to initiate adaptation to the host and express factors such as toxins and siderophores to facilitate access to host iron sources [27],[28].

Candida albicans is a polymorphic yeast which is part of the normal microbial flora of humans. The fungus lives as a harmless commensal on mucosal surfaces in healthy individuals, but can cause several types of infections in predisposed patients, ranging from superficial to life threatening disease [29]. During infection, C. albicans can grow in almost all body sites and organs, indicating an astonishing metabolic flexibility, a high level of stress resistance and effective immune evasion strategies. One of the key features of C. albicans is its ability to grow in different morphological forms – either as ovoid yeast, a filamentous hyphal form or as pseudohyphae [30]. Although the yeast form appears to be important for dissemination [31], the hyphal form is of crucial importance for cell and tissue invasion [32][34]. Furthermore, genes known or proposed to be associated with adhesion, invasion, extracellular hydrolytic activity, detoxification or as yet unknown functions (HWP1, ALS3, SAP4-6, SOD5, HYR1, ECE1) are co-regulated with the yeast-to-hyphal transition [35][40]. Both cellular morphology and expression of hyphal associated genes are tightly regulated by a network of signal transduction pathways (including MAP kinase, cAMP and Rim101 pathways [30],[41]) and transcriptional activators and repressors such as Efg1, Tec1, Bcr1, Tup1 and Nrg1 [42][46].

C. albicans adaptation to the host environment is also reliant on a large number of genes associated with iron acquisition [47]. These genes contribute to the three known iron acquisition systems of C. albicans: (1) Uptake and utilization of iron from hemoglobin is mediated by Rbt5 and Hmx1 [13],[48],[49]. In vitro data have shown that Rbt5 is a hemoglobin receptor that binds hemoglobin on the surface. This binding seems to induce expression of HMX1, which encodes a heme oxygenase. This activity is essential for iron utilization from heme [49]. (2) Iron in siderophores is taken up via the siderophore transporter, Sit1 [50],[51]. C. albicans siderophore production had been demonstrated by biochemical assays in earlier studies [52],[53]. However, in contrast to Aspergillus fumigatus [54], genes encoding factors of a possible siderophore production pathway have not been discovered in the C. albicans genome [47]. Nevertheless, Sit1 can mediate uptake of a range of heterologous siderophores from other organisms and other iron complexes [50], [55][57]. (3) To use free iron from the environment, iron from transferrin, and possibly iron from other so far unknown sources, C. albicans uses the reductive uptake system. This system is located in the plasma membrane and has three components. The first component consists of ferric reductases. At least two surface ferric reductases, which are able to reduce insoluble extracellular ferric (Fe3+) ions into soluble Fe2+ ions, have been described [7],[58],[59]. In addition thirteen homologous genes, putatively encoding other ferric reductases have been identified in the C. albicans genome (http://www.Candidagenome.org). The second component consists of multicopper oxidase. Reduced ferrous iron generated by surface reductase activity can be toxic due to spontaneous generation of free radicals. However, Fe2+ can also be oxidized to Fe3+ by multicopper oxidase activity and thus preventing the production of toxic free radicals [60],[61]. The C. albicans genome contains five putative multicopper oxidase genes [62]. Due to the copper requirement of the oxidase activity, the intracellular copper transporter Ccc2 is essential for this reductive pathway [63]. The third component consists of iron permeases. These form a protein complex with multicopper oxidases and transport Fe3+ into the cell. C. albicans has two iron permeases that are encoded by two highly homologous genes. The high-affinity iron permease gene, FTR1 is induced by iron deprivation and the low-affinity iron permease gene, FTR2 is induced when higher levels of iron are available [64].

All three iron acquisition systems appear to be independent from each other and so far only Ftr1 has been shown to be crucial for C. albicans virulence in an experimental animal model of infection [64]. However, it is unclear which iron sources are used during the different types of C. albicans infection and within different anatomical sites. Recent in vitro and in vivo transcriptional profiling experiments have shown that C. albicans gene expression is tissue specific [33],[34],[65]. Since the relative proportion of iron-containing proteins differs among the different anatomical sites, we propose that usage of iron by C. albicans is niche specific.

Within the oral cavity, extracellular iron is bound mostly to lactoferrin in saliva and intracellular iron is stored in ferritin. However, oral infections by C. albicans are frequent, suggesting that C. albicans must be able to exploit the host iron resources of the oral cavity. We observed that genes encoding the high-affinity reductive iron uptake system of C. albicans are up-regulated during oral infections in patients [33]. Also, C. albicans causes greater damage to oral epithelial cells that contain a high concentration of ferritin (this study). Therefore, we hypothesized that host ferritin may be used as an iron source by this organism. Here we show that C. albicans can utilize iron from ferritin via morphology dependent binding through the adhesin and invasin Als3, suggesting that this single protein has multiple virulence attributes.

Results

The Ferritin Content of Epithelial Cells Influences the Extent of Cellular Damage Caused by C. albicans

C. albicans can attach to and proliferate on oral epithelial tissue and can invade and damage epithelial cells [66]. To elucidate which iron sources are exploited during growth on and invasion of oral epithelial cells and to determine how the availability of iron influences fungal-host cell interactions, we incubated oral epithelial cell monolayers in the presence of additional free iron or the iron chelator bathophenanthrolindisulphonic acid (BPS). This chelator sequesters extracellular, but not intracellular iron [67]. Through immunocytochemical localization of ferritin within epithelial cells, we found that addition of BPS caused a dramatic decrease in cellular ferritin within 24 hours of incubation (Figure 1A), in comparison to non-treated cells (Figure 1B). In contrast, addition of free iron to the medium increased the concentration of ferritin within the same time period (Figure 1C). The treatment with additional iron or the iron chelator itself did not cause cell damage, as monitored by measuring the release of epithelial lactate dehydrogenase (LDH) into the supernatant (not shown).

Figure 1
The ferritin content of host epithelial cells influences the cell damage by C. albicans.

Next, ferritin enriched or depleted epithelial monolayers were incubated for 8 h with C. albicans in normal cell culture medium (serum-free RPMI1640) and cell damage caused by C. albicans was monitored by LDH release. The epithelial monolayers depleted of ferritin were significantly protected from damage in comparison to untreated monolayers (control) (Figure 1D). In contrast, ferritin enriched epithelial cells were significantly and dose dependently more susceptible to damage caused by C. albicans (Figure 1D). These observations suggested that the ferritin content of epithelial cells directly correlates with cell damage and opened up the possibility that C. albicans can use ferritin as an iron source.

Depletion of Ferritin, but not Ferritin Saturation Influences Invasion of Epithelial Cells by C. albicans

To clarify whether the observed increased or decreased cytotoxicity was due to either reduced or increased invasion of epithelial cells by C. albicans, we quantified invasion (after 3 h of co-incubation) in iron depleted versus iron saturated epithelial cells. Invasion of C. albicans into iron depleted epithelial cells was drastically reduced (Figure 2). It is known that C. albicans must invade oral epithelial cells to cause epithelial cell damage [68]. Therefore, the decreased epithelial cell invasion likely contributed to the reduced epithelial cell damage caused by iron depletion. In contrast, iron saturated epithelial cells were invaded at the same proportion as compared to untreated cells (Figure 2), even though C. albicans caused much more damage to these cells. These results suggest that the iron content of epithelial cell influences their susceptibility to damage by C. albicans, a mechanism that is at least partially independent of the extent of epithelial cell invasion.

Figure 2
Invasion of ferritin depleted or enriched epithelial cells by C. albicans.

C. albicans Can Use Ferritin as the Sole Source of Iron in vitro

One explanation for the increased susceptibility of iron saturated epithelial cells to damage by C. albicans is that the organism uses epithelial cell ferritin as an iron source and is thereby able to produce more cytotoxic factors. To test whether C. albicans can use ferritin as an iron source in vitro, we incubated fungal cells on agar with ferritin as the sole iron source. By addition of BPS to the medium, we were able to remove any residual iron from the agar, medium or plastic surfaces. Only the addition of an external iron source allowed fungal growth under these conditions. Moreover, to minimize possible iron contamination of the ferritin solutions (not shown), we passed the ferritin through a column (Microcon YM-100, see Material and Methods) and washed it once with 5 mM HEPES buffer (pH 7.4) prior to use. Addition of free ferric iron, hemoglobin or ferritin to the agar promoted the growth of C. albicans at pH 7.4 (Figure 3A). In contrast, the baker's yeast Saccharomyces cerevisiae, known to be unable to grow with hemoglobin as the sole source of iron [13], only grew after addition of free iron to the medium (Figure 3A). However, S. cerevisiae was able to grow with ferritin when the initial pH of the medium was calibrated to pH 5.0 (not shown). This result suggested that the external pH of the medium influenced the bio-availability of iron from ferritin.

Figure 3
Usage of ferritin by C. albicans requires the reductive pathway and is mediated by acidification of the medium.

The Use of Ferritin as the Sole Source of Iron in vitro Requires the Reductive Pathway and is Mediated by Acidification of the Medium

The ferritin protein shell is unstable at acidic pH [18]. Therefore, our finding that S. cerevisiae can utilize iron from ferritin at acidic, but not alkaline pH, suggested the possibility that C. albicans is able to release iron from this protein by active acidification of the medium. In fact, C. albicans was able to acidify a medium buffered with 25 mM HEPES (initial pH 7.4) during incubation with ferritin as sole source of iron as monitored by the pH indicator bromocresol green (Figure S1). To determine whether ferritin utilization is dependent on fungal-driven acidification, we substituted the glucose in the medium for casamino acids. This mixture of amino acids can be used as a carbon source by yeasts and avoids the acidification associated with glucose use [69],[70]. Furthermore, we stabilized the buffering capacity of the medium by the addition of HEPES buffer (pH 7.4) with increasing concentrations. As shown in Figure 3B, decreasing the capacity to acidify the medium, reduced the ability of C. albicans to grow with ferritin as a sole source of iron.

Next, we sought to determine which of the three known iron uptake systems of C. albicans are involved in iron acquisition from ferritin. Mutants lacking key genes of each iron acquisition system were screened for growth on ferritin agar plates. A mutant lacking the high-affinity permease Ftr1 was able to grow with free iron, hemoglobin, but not with ferritin as the sole iron source (Figure 3A; Table 1). Similarly, a mutant, lacking the copper transporter Ccc2, which is also essential for the reductive pathway, did not grow on ferritin plates (Figure S2; Table 1). In contrast to S. cerevisiae, Δftr1 and Δccc2 mutants did not grow on ferritin plates even when the initial pH was reduced to 5.0 (not shown). As expected, the Δftr1+FTR1 and Δccc2+CCC2 re-integrant strains grew similarly to the wild-type strain in the presence of ferritin (Figure S2). These observations suggest that the reductive pathway is essential for C. albicans to acquire iron from ferritin.

Table 1
Growth of different strains on ferritin agar plates.

The Δsit1 and Δrbt5 mutants grew normally when ferritin was the sole iron source, indicating that C. albicans utilization of iron from ferritin is independent of the siderophore and hemoglobin uptake systems (Figure S2; Table 1).

We also investigated the possibility that aspartic proteases secreted by C. albicans could break down ferritin and release iron. The Δsap1-3 and Δsap4-6 triple-mutants grew similarly to wild-type cells on ferritin plates, suggesting that secreted aspartic proteases of C. albicans are not involved in liberating iron from ferritin (Figure S2; Table 1).

Hyphal, but not Yeast Cells of C. albicans Can Bind Ferritin

Pathogenic microbes frequently utilize iron from host proteins by binding these molecules via specific receptors [4][8],[12],[13],[71]. Since our data showed that C. albicans can use ferritin as a sole source of iron, we investigated whether C. albicans can bind ferritin on its surface.

C. albicans cells precultured in iron limited medium (LIM0) were co-incubated with ferritin and then rinsed extensively. The ferritin that remained bound to the organisms was subsequently visualized with fluorescent labeled anti-ferritin antibodies. Hyphae of wild-type C. albicans bound ferritin whereas yeast-phase organisms did not (Figures 4A and 4B). The binding of ferritin to hyphae was also visualized by electron microscopy. Due to their high-electron density, ferritin molecules appeared as black particles in the electron micrograph adjacent to the fungal cell wall, indicating that ferritin bound to the cell surface, and not within the fungal cell wall (Figure 4C). C. albicans cells incubated under the same condition, but without ferritin, had no such electron dense particles on their surfaces (not shown).

Figure 4
Ferritin binding of C. albicans requires hyphal formation.

The finding that ferritin was not bound by either yeast cells or the mother cells of hyphae suggested that ferritin binding was specific to C. albicans hyphae. To test this hypothesis further, we investigated the ferritin binding of C. albicans Δras1 and Δcph1/efg1 mutants that were unable to form hyphae, and did not express hyphal-specific genes [72],[73]. Both mutants were unable to bind ferritin (Figure 4A and 4B). Next, we tested the ferritin binding capacity of a Δhgc1 mutant, which forms pseudohyphae rather than true hyphae, but still expresses hyphal-specific genes [74]. When grown under hyphal-inducing conditions (RPMI 1640, 37°C with 5% CO2), the Δhgc1 mutant bound ferritin even though it did not form true hyphae (Figure 4A and 4B). These results suggest that the product of one or more hyphal specific genes is essential for C. albicans to bind ferritin.

To uncover which hyphal-associated activities are involved in ferritin binding, wild-type hyphae were killed with thimerosal or UV light and tested for ferritin binding. Cells killed with thimerosal still bound ferritin; whereas cells killed with UV light did not (Figure S3A). When untreated wild-type cells were mixed with 50% UV-killed cells, we observed 49.06%±4.27% ferritin binding. These data demonstrate that cell viability is not necessary for ferritin binding and that the ferritin receptor on the cell surface is inactivated by UV treatment. We also investigated whether iron availability influenced the extent of ferritin binding of wild-type hyphae. Cells grown under iron limiting conditions or in the presence of excess iron bound ferritin similarly (Figure S3B). Also, C. albicans hyphae were able to bind ferritin and apoferritin (a ferritin shell without iron) with similar efficiency (not shown) indicating that iron molecules within the ferritin shell were dispensable for binding of ferritin. Thus, these data indicated that the binding of ferritin by C. albicans is morphology associated, but not iron-regulated.

Transcriptional Profiling of C. albicans Cells Binding Ferritin Identifies Putative Genes Necessary for Ferritin Binding

Transcriptional profiling was used to identify genes encoding putative ferritin receptors. We incubated a wild-type strain (true hyphae and ferritin binding), the Δhgc1 mutant (yeast or pseudohyphae and ferritin binding) and Δras1 (no hyphae and no ferritin binding) under hyphal-inducing conditions (RPMI medium, 37°C with 5% CO2) and in the presence of ferritin. After 1.5 h, the RNA from all three strains was isolated, labeled and hybridized to C. albicans microarrays. Microarray data from four independent experiments were analyzed. We reasoned that candidate genes encoding putative ferritin receptors should be up-regulated in wild-type and Δhgc1 cells, but should be unaltered or down-regulated in the Δras1 mutant (Figure 5). A total of 22 genes were identified with such an expression profile (Figure 5). Expression data shown in Table 2 indicate the genes that were up-regulated in wild-type cells but not in Δras1 mutant cells. Three of these genes were known to encode hyphal-specific proteins that are cell surface localized as would be expected for a receptor protein. Consequently, these three genes were further investigated.

Figure 5
Transcription profiling identifies genes associated with ferritin binding.
Table 2
Genes up-regulated in wild-type and Δhgc1 cells, but unaltered or down-regulated in the Δras1 mutant (Figure 5).

Deletion of ALS3 Precludes Ferritin Binding

The three genes encoding cell surface localized and hyphal-specific proteins were ECE1, HYR1 and ALS3. ECE1 (Extent of Cell Elongation) is a hyphal-specific gene with yet unknown functions. ECE1 expression increases during elongation of the hyphal cell. This gene encodes a predicted cell membrane protein and the corresponding null mutant displays no obvious altered phenotype [40]. HYR1 (HYphally Regulated) encodes a GPI-anchored protein that is predicted to be cell wall localized and is of unknown function [39]. Finally, ALS3 (Agglutinin-Like Sequence) encodes a hyphal-specific cell wall protein which belongs to a family of adhesins (Als family) [75] and plays a crucial role in epithelial and endothelial adhesion and invasion [32].

The corresponding homozygous null mutants were tested for ferritin binding. Both, the Δece1 and the Δhyr1 mutants bound ferritin similarly to the wild-type strain (Figure 6A and B). In contrast, ferritin binding of the Δals3 mutant was dramatically reduced (Figures 6 and and7).7). This defect in ferritin binding was restored when a wild-type copy of ALS3 was reintegrated into the Δals3 mutant (Figures 6 and and77).

Figure 6
Als3 is essential for ferritin binding.
Figure 7
Flow cytometric detection of ferritin binding.

These results suggested that Als3 plays a crucial role in ferritin binding and may in fact be the hyphal-specific ferritin receptor.

Upstream Regulators of ALS3 are Required for Ferritin Binding

If Als3 is a ferritin receptor, one would expect that mutants lacking factors that govern ALS3 expression would also have an altered capacity to bind ferritin. Therefore, we tested two mutants that lacked ALS3 transcriptional regulators. BCR1 encodes a transcription factor which regulates the expression of certain hyphal-specific genes, including ALS3 [76]. Furthermore, expression of BCR1 itself depends on Tec1 [44]. Figure 6C shows that the presence of both transcriptional factors, Tec1 and Bcr1, is necessary for C. albicans cells to bind ferritin. These data reinforce the view that Als3 plays a key role in the capacity of C. albicans to bind ferritin.

Binding is Necessary for Iron Acquisition from Ferritin

To determine whether ferritin binding is necessary for the utilization of iron from this protein, we tested the growth of the Δals3 mutant with ferritin as the sole iron source. The Δals3 mutant grew very poorly on agar plates (pH 7.4) with ferritin as the sole source of iron (Figure 8). The reconstitution of one copy of the gene (Δals3+ALS3 re-integrant strain), improved growth, although not to wild-type levels (Figure 8). Growth of the Δals3 mutant in media with low iron content was not reduced, indicating that uptake of free iron is normal in the Δals3 mutant (not shown). Therefore, Als3 is required for C. albicans hyphae to both bind and utilize ferritin as a source of iron.

Figure 8
Binding is necessary for iron acquisition from ferritin.

Moreover, a mutant unable to form hyphae (Δras1) and thus unable to bind ferritin was also tested for growth on ferritin plates. As expected, Δras1 displayed a reduced ability to grow with ferritin as the sole source of iron (Figure S4). This result reforces the key role of hyphal development and the hyphal associated expression of ALS3 in the ability of C. albicans to obtain iron from ferritin.

Als3 is a Ferritin Receptor

To elucidate whether Als3 itself can bind ferritin without an additional C. albicans surface factor, we tested the ferritin binding capacity of a strain of S. cerevisiae that expressed C. albicans ALS3 [77]. Because ALS3 is a member of a large gene family encoding similar proteins, we also analyzed two additional S. cerevisiae strains that expressed ALS1 or ALS5, two closely related ALS genes. The strain that expressed ALS3 strongly bound ferritin, whereas the strains that expressed ALS1 or ALS5 did not (Figure 9).

Figure 9
Als3 is a ferritin receptor.

Invading C. albicans Hyphae Bind Ferritin from Epithelial Cells during Infection

Next we investigated whether ferritin binding via Als3 occurs when C. albicans interacts with host cells. Oral epithelial cells were loaded with iron and then incubated with wild-type C. albicans, the Δals3 mutant, or the Δals3+ALS3 re-integrant strain for 6 h.

To visualize ferritin molecules on cellular surfaces and to investigate whether the location of fungal cells had an influence on ferritin binding, we used an immunofluorescence approach with differential staining, which enabled us to discriminate between hyphae located on the epithelial cell surface and hyphae that had invaded into the epithelial cells (Figure 10 columns 1, 2 and 4). In addition, we used an anti-ferritin antibody to localize ferritin (Figure 10 column 3). As shown in Figure 10, hyphae of wild-type and Δals3+ALS3 re-integrant strains invaded the epithelial cells and were surrounded by ferritin (white arrows in Figure 10). Very little ferritin accumulated around wild-type hyphae that had not invaded the epithelial cells (data not shown). In contrast, the few hyphae of the Δals3 mutant that invaded the epithelial cells displayed no accumulation of ferritin (Figure 10G and K). These results indicate that C. albicans hyphae bind to ferritin in an Als3-dependent manner while invading epithelial cells.

Figure 10
C. albicans hyphae invading oral epithelial cells bind ferritin.

C. albicans Mutants Lacking Genes Essential for Iron Utilization from Ferritin are Unable to Damage Epithelial Cells

If binding to ferritin and utilizing host iron are important for C. albicans to cause an oral infection, one would expect that mutants lacking ALS3 or FTR1 would have a reduced potential to cause tissue damage as compared to wild-type cells. To test this prediction, we measured the extent of epithelial cell damage caused by wild-type, Δals3 mutant and Δftr1 mutant strains of C. albicans. We found that the Δals3 and Δftr1 mutants lost their capacity to damage epithelial cells (Figure 11). In contrast to Δals3 mutant cells, which displayed strongly reduced invasion abilities when co-incubated with epithelial cells for 3 hours (not shown), Δftr1 mutant cells showed the same invasion rate than the wild-type strain (Figure S5). Although hyphae of this mutant seemed shorter than the wild-type hyphae, there was no morphological differences between Δftr1 mutant cells on epithelial cells and in RPMI medium alone (control) (not shown).

Figure 11
Iron uptake from ferritin plays a role in oral epithelial cell damage.

Discussion

Iron availability is a critical factor for all pathogenic microbes and iron excess can accelerate pathogenicity [1], [78][80]. We observed that oral epithelial cells enriched in intracellular ferritin were more susceptible to tissue damage by wild-type C. albicans and that epithelial cells depleted of ferritin were significantly protected from damage. The reduced damage of iron depleted epithelial cells correlated with reduced invasion of C. albicans. It is possible that the treatment with the iron chelator affected both the host cells and the pathogen. Iron depleted epithelial cells may have a reduced ability to internalize fungal cells and limited accessibility of iron may reduce the capacity of C. albicans to both invade and damage epithelial cells. This model is supported by previous data. For example, endothelial cells incubated with an iron chelator before C. albicans infection were protected from injury by C. albicans [81] and the anti Candida activity of ciclopiroxolamine, a potent antifungal agent, is proposed to be mediated by iron chelation [82][84]. In contrast, when epithelial cells were loaded with exogenous iron, epithelial cell uptake of C. albicans was not affected. However, the increased iron reservoir was likely exploited by C. albicans, leading to increased epithelial cell damage. This increased damage was probably due to an enhanced production of virulence determinants (e.g. hydrolases) and hyphal extention. Therefore, it can be concluded that access to iron has a direct influence on the pathogenicity of C. albicans, probably by acting on both the host and the fungus. Furthermore, our data suggest that C. albicans is able to directly use ferritin as a source of iron.

It is known that ferritin is an extremely robust and resistant protein. Prior to this study, the only microorganism that has been known to exploit holoferritin as an iron source during interaction with host cells is N. meningitidis [21],[22]. However, this bacterium is not able to directly utilize iron from ferritin. Instead, it induces degradation of cytosolic ferritin by manipulating the host cellular machinery and thereby utilizes the resultant free cytosolic iron. To our knowledge, no published studies have so far demonstrated direct use of iron from host ferritin.

Nevertheless, a number of studies have suggested that certain microbial pathogens can use ferritin as an iron source during in vitro growth. For example, Yersinia pestis can grow on agar containing hemin, myoglobin, hemoglobin or ferritin [85]. A siderophore produced by M. tuberculosis (exochelin) can sequester iron from transferrin, lactoferrin and to a lesser extent from ferritin [86]. L. monocytogenes and Burkholderia cenocepacia can grow in liquid medium with ferritin as the sole source of iron [87],[88]. However, the microbial mechanisms of iron acquisition from ferritin are unknown and it is not clear whether ferritin from host cells can be used by any of these species. Furthermore, although ferritin seems to be almost indestructible under physiological conditions, iron may be released from ferritin in vitro, especially under condition of low pH. In our hands, even S. cerevisiae was able to utilize iron from ferritin under such conditions. Therefore, it is possible that previous observations of the microbial usage of ferritin in vitro were the results of non-physiological conditions.

In contrast to S. cerevisiae, C. albicans can use ferritin as the sole source of iron in vitro even when the growth medium was buffered at a physiological pH. Which mechanisms and activities are involved in iron acquisition from ferritin?

One possibility is that ferritin is degraded by extracellular proteolytic activity since it is known that C. albicans can secrete a family of aspartic proteases (Saps) with very broad substrate specificity [89]. However, it appears that extracellular degradation due to fungal proteases is not necessary for growth with ferritin, since mutants lacking the protease genes SAP1-3 or SAP4-6 were still able to grow on such medium. Indeed, an earlier study by Rüchel demonstrated that ferritin was the only tested protein which was resistant to proteolysis by Sap2, one of the major secreted proteases of C. albicans with an extremely broad substrate specificity [90], supporting the view that proteases are not involved in the ability of C. albicans to utilize iron from ferritin.

Since even S. cerevisiae was able to grow with ferritin when the pH of the medium was low (pH 5.0), we reasoned that the pH plays a crucial role in the release of iron from ferritin. It is known that ferritin is unstable at acidic pH [18] and that the natural recycling of iron from ferritin occurs in the acidic environment of lysosomes [19],[20]. Thus, it may be possible that C. albicans actively lowers the pH in its proximate vicinity. In fact, C. albicans was able to lower the pH of the medium during growth even on buffered ferritin plates (Figure S2). Additionally, the fungus was only able to use ferritin as an iron source under conditions which allowed acid production (glucose, but not casamino acids as a carbon source) and acidification of the surrounding environment (low concentrations of buffer at pH 7.4). Similarly, it has been observed that the bacterial pathogen Staphylococcus aureus, under iron starvation, decreases the local pH resulting in the release of iron from transferrin [91].

It is also possible that C. albicans can produce and secrete reductants, which are able to sequester iron from ferritin. Such a process would indeed be favoured by acidification of the surrounding media. In agreement with this model, reductants or chelators such as thioglycolic acid, ascorbate, and aceto- and benzohydroxamic acids are capable of releasing iron from the ferritin core [92][94]. Underscoring the importance of pH in the release of iron from ferritin, this process is increased at pH 5.2 in comparison to pH 7.4 [94]. However, since we demonstrated that binding is necessary for ferritin iron exploitation by C. albicans, it can be hypothesized that a surface factor rather than a secreted factor is necessary for ferric iron reduction from the ferritin core. Another possible speculation is that reductases on the C. albicans cell surface can reduce ferric iron from the ferritin core and that this process may be facilitate under acidic pH.

Although we do not have experimental evidence that local acidification occurs in vivo during infection, transcriptional profiling of C. albicans during experimental infections suggests that the local environment of at least some cells in fact changes from neutral to acidic pH during invasion and tissue damage. For example, we have found that the acid induced gene, PHR2 is up-regulated during tissue invasion [34].

In addition to the ability to acidify the environment, C. albicans requires the reductive high-affinity iron uptake pathway to exploit iron from ferritin. Mutants lacking either the high-affinity permease Ftr1 or the copper transporter Ccc2 (which is essential for the reductive pathway) [63],[64] did not grow on ferritin plates even when the initial pH was low. Therefore, we conclude that a combination of active acidification and uptake via the high-affinity permease are key mechanisms in this process. As a third prerequisite, we hypothesized, that a close association between C. albicans cells and ferritin is required for the release of iron from ferritin and subsequent uptake into the fungal cell. This close contact is facilitated by binding of ferritin on the fungal surface.

In principle it may also be postulated that a yet unknown molecule is secreted by C. albicans, which binds ferritin and subsequently delivers the iron protein to a surface receptor, similar to some bacteria which can secrete haemophores that bind extracellular haemoglobin and mediate its delivery to surface receptors [95]. However, such a mechanism is unlikely to be involved in ferritin-binding by C. albicans since fungal cells that were killed with thimerosal and then washed, removing any secreted factors, were still able to bind ferritin.

Interestingly, fungal cells killed via exposure to UV-light lost their ability to bind ferritin. This result suggests that ferritin-binding at the cell surface is mediated by a receptor which is inactivated by UV treatment. In support of this possibility, it is known that certain proteins can be inactivated by exposure to UV light [96].

Several lines of evidence suggest that the cell surface protein, Als3 is a receptor that binds ferritin and facilitates iron acquisition from this protein. (1) Only hyphae, but not yeast cells bound ferritin and Als3 is known to be a hyphal-specific protein. However, the binding of ferritin did not need the hyphal morphology, since a mutant lacking Hgc1 [74] did not produce true hyphae, but still bound ferritin (Figures 4A and 4B) and expressed ALS3 (not shown). (2) Mutants lacking transcription factors known to regulate ALS3 expression (Tec1, Bcr1) [44],[45] had a reduced ability to bind ferritin. In agreement with this, a mutant that was unable to form hyphae (Δras1) and that did not express ALS3, also displayed reduced binding of ferritin and reduced growth on ferritin plates. (3) A mutant lacking ALS3 was dramatically reduced in its ability to bind ferritin and displayed poor growth on ferritin plates. The Δals3+ALS3 re-integrant strain had a restored ability to bind ferritin and a partially restored ability to grow on ferritin plates, although not to wild-type levels, possibly due to a gene dosage effect. Finally, (4) a S. cerevisiae strain expressing Als3 was able to bind ferritin.

Binding of ferritin to hyphal surfaces was observed with both exogenously added purified ferritin and during the interaction of C. albicans with intact epithelial cells. Only hyphae, but not yeast cells showed bound ferritin during interaction with epithelial cells. Furthermore, ferritin accumulation was predominantly observed on those hyphae that had invaded the epithelial cells. Finally, the hyphae of the Δals3 mutant did not show ferritin accumulation.

Taken together, these data suggest that ferritin can be used as an iron source by C. albicans via direct binding by Als3 on the surface of hyphae, iron release is then mediated by acidification and uptake is facilitated by the reductive pathway (Figure 12).

Figure 12
Proposed model for iron utilization from ferritin by C. albicans.

Although we do not have direct evidence that ferritin is in fact used as an iron source during interaction with epithelial cells, these data at least suggest that ferritin is in close contact to invading C. albicans hyphae and thus may be exploited by the above proposed mechanism. This view is supported by the fact that both the Δals3 mutant and the Δftr1 mutant completely lost their capacities to damage epithelial cells in vitro. Furthermore, the Δals3 mutant has significantly reduced capacity to damage epithelial cells in the reconstituted human epithelium model [97]. However, it should be noted that this reduced damage is likely due to a combination of reduced adherence [97], reduced invasion [32], and reduced ability to use ferritin as an iron source. Interestingly, hyphae of the Δftr1 mutant displayed the same invasion rate than the wild-type strain, suggesting that this mutant can initially invade the epithelial cells, but is not able to damage host cells possibly because it can not use ferritin as an intracellular available iron source.

Several studies have shown that pathogenic microbes link the availability of iron with virulence attributes. In this study, we show that a similar link between the regulation of an iron acquisition system and virulence attributes exists in C. albicans. In fact, the regulation of the ferritin receptor Als3 is independent from external iron sources and seems to be strictly linked to hyphal formation, one of the most extensively investigated virulence attributes of C. albicans [30],[98].

Therefore, iron acquisition of the intracellular iron storage protein ferritin is hyphal regulated. Hyphal formation is also associated with adhesion, proteolytic activity, cellular invasion and damage [32][34],[89],[99], and the hyphal form of the organism is the predominant morphology that reaches the intracellular compartments of epithelial cells where ferritin is located. Therefore, C. albicans co-regulates morphology, invasion, tissue damage and an iron acquisition system. This view may explain why iron acquisition from ferritin is a hyphal-specific property and does not occur with the normally non-invasive yeast cells.

A second potential link exists between the external pH, hyphal formation and iron acquisition. It is well known that the external pH influences hyphal formation [100],[101] and we recently reported that pH-dependent hyphal formation is crucial for liver invasion [34]. During liver invasion C. albicans cells are exposed to a neutral or alkaline pH and iron limited conditions as reflected by transcriptional profiles [34]. Availability of iron for fungal cells within a human host is even more difficult in neutral or slight alkaline pH conditions such as those found in the liver tissue (pH 7.4) because the balance between the soluble Fe2+ ion and the insoluble ferric form Fe3+ shifts towards the insoluble form [41]. Therefore, the formation of hyphae and expression of Als3 in response to neutral pH may facilitate iron acquisition by C. albicans.

Interestingly, the expression of ALS3 is not absolutely linked to the hyphal morphology in wild-type cells. Sosinska et al. [102] recently observed that hypoxic conditions and iron restriction in a vagina-simulative medium affected cell morphology and the cell wall proteome of C. albicans. One of the proteins found in yeast cells under these iron limited conditions was Als3, which indicates that even proteins which are strictly hyphal-associated under most growth conditions, may be expressed in the yeast form. Similarly, White and co-workers recently showed that C. albicans expresses a number of hyphal-specific genes (such as ECE1) in a murine gut model of commensalism, whilst growing in the yeast morphology [103]. The observation that yeast cells express Als3 under iron limited conditions may further support the view that this protein is involved in iron acquisition from the host. However, the expression of ALS3 is not directly linked to low iron conditions since two studies that analyzed the influence of iron on the genome wide gene expression of C. albicans [47],[83] found that iron starvation did not increase the expression of ALS3.

The Als protein family of C. albicans encodes large cell-surface GPI-glycoproteins that were originally implicated in the process of adhesion to host surfaces [75],[104]. Expression of Als3, was shown to be hyphal-specific [36] and was observed in vivo during oral and systemic infection [33],[34]. In addition to its adhesion properties, Als3 was recently shown to be an invasin that binds to cadherins and induces endocytosis by host cells [32]. In this study, we made the intriguing observation that Als3 has a third function in iron acquisition by binding to host ferritin, indicating that this single member of a protein family has multiple virulence-associated functions.

Materials and Methods

Fungal growth conditions and strains

C. albicans were grown in liquid YPD medium (1% yeast extract [Merck, http://www.merck.de], 2% bactopeptone [Difco, http://www.bdbiosciences.com], and 2% D-glucose [Roth, http://www.carl-roth.de]) in a shaking incubator at 30°C for 8 h. Subsequently, the cultures were diluted 1[ratio]1000 in LIM0 medium [105] and incubated in a shaking incubator at 30°C overnight for iron starvation. For non-starved cells, precultures were incubated in YPD medium overnight at 30°C with shaking. The yeast cells were harvested by centrifugation, washed three times in filter sterilized ultra-pure water and counted using a hemacytometer. Strains of C. albicans and S. cerevisiae used in this study are listed in Table 3 and Table 4, respectively.

Table 3
C. albicans strains used in this study.
Table 4
S. cerevisiae strains used in this study.

Oral epithelial cells

The epithelial cell line TR146, derived from a squamous cell carcinoma of buccal mucosa [106], was kindly provided by Cancer Research Technology (http://www.cancertechnology.co.uk). TR146 cells were routinely grown in RPMI 1640 medium (PAA, http://www.paa.com) supplemented with 10% fetal bovine serum (FBS; PAA). For experiments, epithelial cells were used between passages 10 to 20. Monolayers with 70–90% confluent cells in 24 well plates were additionally incubated for 24 h in three different conditions: (1) RPMI 1640 with 50 µM bathophenanthrolinedisulfonic acid disodium salt (BPS; iron chelator; Sigma-Aldrich, http://www.sigmaaldrich.com); (2) RPMI 1640 with 10% FBS; (3) RPMI 1640 with 10% FBS and indicated concentrations of iron chloride (FeCl3; Merck). After 24 h incubation, monolayers were washed twice with phosphate-buffered saline without calcium or magnesium (PBS) and serum-free RPMI 1640 medium was added. Each well was infected with ~106 C. albicans cells and incubated for 8 h. Supernatants were removed for LDH measurements. All incubations were performed in a humidified incubator at 37°C in 5% CO2.

To monitor the ferritin content of cells, the uninfected monolayers were fixed with Roti®-Histofix 4% (Roth) and the ferritin content of the cells was visualized under the microscope using immunofluorescence. Briefly, fixed monolayers were permeabilized through incubation with 0.1% Triton X-100 (Serva, http://www.serva.de) for 15 min at room temperature and washed three times with PBS. Next, the samples were blocked using Image-iT™ FX signal enhancer (Invitrogen, http://probes.invitrogen.com/products/) for 30 min at room temperature in a humidity chamber. Cells were again washed three times with PBS and incubated with rabbit anti-ferritin antibody (Sigma-Aldrich) coupled with dye DY-649 (Dyomics, http://www.dyomics.com) diluted 1[ratio]1000 in PBS with 1% bovine serum albumin (BSA, Sigma-Aldrich) for 1 h at room temperature. Finally, cover-slips were washed three times with PBS, inverted and mounted on a microscope slide with ProLong® Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Invitrogen). The samples were analyzed in duplicates using a Leica DM 5500B microscope (Leica, http://www.leica-microsystems.com). The same exposure time and light intensity were used to analyze all samples, permiting comparisons. For every sample, 10 randomly chosen fields per cover-slip were photographed using a DFC 350 FX camera (Leica). A representative picture of each condition was selected.

Epithelial cell monolayer damage assay

Epithelial cell damage caused by C. albicans, was determined by the release of lactate dehydrogenase (LDH) into the medium using a Cytotoxicity Detection Kit–LDH (Roche, http://www.roche.de). The assays were performed according to the manufacturer instructions and the measurements were performed in duplicates.

To measure epithelial cell damage, the following calculation was used: 100×(ECa−C1−C2)/(100L−C1) = relative cytotoxicity (%). Absorbance measured at OD 490–600 directly correlates with LDH activity. ECa = epithelial cells infected with C. albicans; C1 = control 1–uninfected epithelial cells; C2 = control 2–only C. albicans; 100L = 100% lysis (0.2% Triton-X 100, Serva). Controls 1, 2 and 100% lysis were determined individually for each treatment.

Ferritin agar plates

To investigate whether C. albicans was able to grow with ferritin as the sole source of iron, we added 350 µM BPS to the SD agar medium (6.7 g/l yeast nitrogen base, YNB [Difco]; 20 g/l D-glucose; 20 g/l purified agar [Oxoid, http://www.oxoid.com]). Additionally, HEPES buffer (Sigma-Aldrich) was added to the medium as indicated and the pH was adjust to 7.4 using a 5 M NaOH stock solution (Roth). To prevent active acidification of the medium by the fungus, 20 g/l casamino acids (Difco) was used in place of D-glucose. The ferritin solution (ferritin from horse spleen [Sigma-Aldrich]) was diluted 1[ratio]100 in a dilution buffer (5 mM HEPES; 0,1 M NaCl [Roth]) and passed through a Microcon YM-100 Centrifugal Filter Unit (Millipore, http://www.millipore.com). The retentate was collected in a fresh 1.5 ml microcentrifuge-tube and the original volume was adjusted with the dilution buffer. Afterwards, this ferritin solution was plated out on agar surfaces at indicated concentrations. To monitor the pH changes in the medium during C. albicans growth, the pH indicator bromocresol green (Sigma-Aldrich) was added to the medium at a concentration of 3.9 mg/l.

Ferritin binding assay

C. albicans cells growing under iron limitation, as described above, were washed and enumerated. Approximately 5×105 cells were added per well in a 24 well plate (TPP, http://www.tissue-cell-culture.com) containing Poly-L-Lysine-coated (Biochrom AG, http://www.biochrom.de) 12-mm diameter glass cover-slips and 1 ml RPMI 1640. The cells were incubated for 3 h at 37°C under 5% CO2 to induce hyphae. Afterwards, the cells were washed once with PBS and incubated for 1 h in 1 ml PBS with 1% bovine serum albumin (BSA) and 100 µg/ml ferritin. Subsequently, the cells were washed three times with PBS to remove non-bound ferritin and fixed with 500 µl Roti®-Histofix 4%.

To test if viability is necessary for ferritin binding, C. albicans hyphae (3 h in RPMI 1640 at 37°C and 5% CO2) were killed using two different approaches: either 1.5 h incubation at room temperature with 0.05% Thimerosal (Sigma-Aldrich) or 2 times exposition to 0.5 J/cm2 UV light in a UV-crosslinker with a 254 nm low pressure mercury-vapor lamp (Vilber-Loumart, http://www.vilber.de). Complete killing without residual viability of cells was checked by plating the cells on YPD agar plates. After killing, the cells were incubated with ferritin and fixed as described above.

The fixed cells were washed three times with PBS and incubated with rabbit anti-ferritin antibody coupled with dye DY-649 diluted 1[ratio]2000 in PBS with 1% BSA for 1 h at room temperature. Next, the cover-slips were inverted and mounted on a microscope slide with ProLong® Gold Antifade Reagent (Invitrogen) and cells were visualized using a Leica DM 5500B microscope (Leica). Photomicrographs were taken using a DFC 350 FX camera (Leica). To quantify how many C. albicans cells bound ferritin, at least 100 cells per cover-slip were counted and percent binding was calculated by counting the total number of cells and the number of cells displaying fluorescent signal. All binding assays were performed in duplicates. Cells incubated without ferritin were used as a negative control.

Because S. cerevisiae cells were detached during the washing steps described above, a different approach was used. The use of a fluorophore-coupled ferritin reduced the number of washing steps in the staining procedure and consequently left more cells on the coverslip for observation by fluorescent microscopy. Briefly, 5×105 cells were added per well in a 24 well plate containing Poly-L-Lysine-coated 12-mm diameter glass cover-slips in 1 ml RPMI 1640. The cells were incubated for 1 h at 30°C. Afterwards, the medium was removed and 250 µl PBS with 1% BSA and 25 µg/ml ferritin coupled with dye DY-649 was added. After 15 min at 30°C, the cells were washed once with PBS, fixed, mounted and visualized under the microscope as described above for C. albicans cells.

Transmission electron microscopy

C. albicans wild-type cells (SC5314) were grown on poly-L-lysine-coated cover-slips (0.5 mm in diameter) in the presence or absence of 100 µg/ml ferritin for 6 h in RPMI 1640. Afterwards, the cells were washed with PBS four times to remove non-bound ferritin and then immersed in fixative (4% formaldehyde, prepared from para-formaldehyde [Roth] and 0.1% glutaraldehyde [Roth] in 0.05 M HEPES ) at room temperature. After three min the fixative was replaced with fresh fixative and stored at 4°C overnight. The samples were dehydrated in ethanol (Roth) by progressively lowering the temperature to −35°C and infiltrated with Lowicryl K4M resin (Polysciences, http://www.polysciences.com) at −35°C [107]. The resin polymerization was carried out under UV light at −35°C for 24 h and for 10 h at 0°C. Ultra thin sections (60–80 nm thick) were produced with an Ultracut S (Leica) and a diamond knife. Sections were collected on formvar filmed copper slot grids. Bright-field transmission electron microscopy was performed with an EM902 (ZEISS, http://www.zeiss.de) at 80 kV. Images were recorded with a 1 k CCD camera (Proscan, http://www.proscan.de).

Flow cytometry analysis of ferritin binding

Flow cytometry was used to quantify the binding of ferritin on the surface of C. albicans hyphal cells. C. albicans cells were grown under iron limitation, as described above, washed and counted. Approximately 106 cells in 1 ml RPMI 1640 medium were added to poly-L-lysine-coated (Biochrom) 12-mm diameter glass cover-slips in a 24 well tissue-culture plate (TPP). The cells were incubated for 2 h at 37°C in 5% CO2 to induce hyphae. Next, the cells were washed once with PBS and incubated for 1 h in 0.5 ml PBS with 1% bovine serum albumin (BSA) and 100 µg/ml ferritin. Subsequently, the cells were washed three times with PBS to remove non-bound ferritin and fixed with 500 µl Roti®-Histofix 4%. The fixed cells were washed three times with PBS and incubated with rabbit anti-ferritin antibody (Sigma-Aldrich) diluted 1[ratio]500 in PBS with 1% BSA for 1 h at room temperature. After washing, the cells were incubated with a goat anti-rabbit secondary antibody conjugated with Alexa 488 (Invitrogen) diluted 1[ratio]500. Finally, the cells were detached from the cover-slips using a pipet point and resuspended in 0.5 ml PBS. The fluorescent intensity of the hyphae was measured using a LSRII flow cytometer (Becton Dickinson, http://www.bd.com). Fluorescence data for 10,000 cells of each strain were collected.

Immunofluorescence of infected epithelial cells

Ferritin enriched epithelial cell monolayers (described above) were washed twice with PBS and infected with ~105 C. albicans cells in serum-free RPMI 1640 medium for 6 h. Next, the samples were washed twice with PBS and fixed with 500 µl Roti®-Histofix 4%. C. albicans cells and TR146 cells were incubated separately and used as controls. All incubation times were performed in a humidified incubator at 37°C in 5% CO2.

To stain C. albicans cells localized only outside epithelial cells, before permeabilization, the samples were incubated with 12.5 µg Concanavalin A–fluorescein conjugate (Invitrogen) in PBS for 45 min at room temperature. After washing, the cells were permeabilized by incubation with 0.1% Triton X-100 for 15 min at room temperature. After washing three times with PBS, the samples were blocked using Image-iT™ FX signal enhancer (Invitrogen) for 30 min at room temperature in a humidity chamber. After washing three times with PBS, the cells were incubated with rabbit anti-ferritin antibody coupled with dye DY-649 diluted 1[ratio]1000 in PBS with 1% BSA for 1 h at room temperature. To stain C. albicans cells localized outside and inside epithelial cells, the samples were incubated with 10 µg/ml Calcofluor White (Sigma) in 0,1 M Tris-hydrochloride (pH 9.0 [Roth]) for 20 min at room temperature.

Finally, cover-slips were washed three times with ultra pure water, inverted and mounted on a microscope slide with ProLong® Gold Antifade Reagent. At least two experiments in duplicates were analyzed using a Leica microscope and 10 randomly chosen fields per cover-slip were photographed. A representative picture of each strain was selected.

Invasion of ferritin depleted or enriched oral epithelial cells

Ferritin depleted or enriched oral epithelial cell monolayers (as described above) were washed twice with PBS and infected with ~105 iron starved C. albicans cells in serum-free RPMI 1640 medium for 3 h. The samples were washed twice with PBS and fixed with 500 µl Roti®-Histofix 4%. C. albicans cells alone were incubated separately and used as control. All samples were incubated in a humidified incubator at 37°C and 5% CO2. The samples were stained to distinguish invading from non-invading fungal cells as described above. At least 100 randomly selected organisms were analyzed and the percentage of organisms that had invaded the epithelial cells was calculated.

Sample preparation for RNA extraction

C. albicans cells growing under iron limitation, as described above, were washed and enumerated. Approximately 2×106 cells were added per well in a 24 well plate containing Poly-L-Lysine -coated 12-mm diameter glass cover-slips in 1 ml RPMI 1640 with 100 µg/ml ferritin. The strains used were CAI4 carrying CIp10; Δhgc1 carrying CIp10 and Δras1 carrying CIp10. The plasmid CIp10 was used to reconstitute URA3 into the RP10 locus of each strain [108]. After 1.5 h incubation at 37°C under 5% CO2, the medium was removed and 100 µl peqGOLD RNAPure (PeqLab, http://www.peqlab.de) was added per well. The cells were immediately removed from the cover-slips using a pipette point. For each strain, cells from 12 wells were pooled in a microcentrifuge tube and immediately shock frozen in liquid nitrogen. To verify that ferritin was bound to C. albicans hyphae as observed before, additional cover-slips for each strain were fixed and ferritin was stained as described.

RNA extraction and labeling

Frozen cells were lysed and homogenized (Precellys 24, PeqLab) with glass beads (0.5 mm, Roth). Total RNA was extracted as previously described [109]. Total RNA was linearly amplified and labeled using the ‘Low RNA Input Fluorescent Linear amplification Kit’ (Agilent Technologies, http://www.agilent.de).

Microarray hybridization and analysis

For transcriptional profiling, C. albicans microarrays (Eurogentec) were used as previously described [109]. RNA was co-hybridized with a common reference (RNA from SC5314 grown in YPD medium, mid-log phase, 37°C). Slides were hybridized, washed and scanned as described [109]. Data normalization (LOWESS) and analysis were performed in Gene-Spring 7.2 software (Agilent Technologies). Reliable expression of genes was defined as normalized expression of present genes that did not vary more than 1.5 standard deviations within replicate arrays. Genes were defined as differentially expressed if their expression was at least 2 times stronger or 2 times weaker in at least one strain compared to the common reference. Using the Benjamini and Hochberg false discovery test, a p-value<0.05 was considered as significant. Microarray data from four independent experiments (two of them with dye swap) were used. To identify genes involved in ferritin binding, genes were selected that were up-regulated (≥2.5 increase in expression compared to the common reference) in wild-type and Δhgc1 cells, but unaltered or down-regulated (≤1.5 of the common reference expression) in the Δras1 mutant. Raw data have been deposited in NCBIs Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) and are accessible through GEO series accession number GSE11490.

Statistical Analysis

Statistical significances (p-values) were calculated with the Student's two-tailed t-test function in Microsoft Excel, with exception of the microarray analysis described above.

Supporting Information

Figure S1

C. albicans can acidify the medium during growth on ferritin plates. C. albicans wild-type (SC5314) was grown on media containing ferritin as the sole source of iron and bromocresol green (3.9 mg/ml) as a pH indicator. SD agar was buffered using 25 mM HEPES (pH 7.4). BPS, iron chelator; ferritin, 15 µg/ml ferritin. All plates were incubated for 4 days at 37°C under 5% CO2. Blue indicates pH values higher than 5.5. Green indicates pH values between 5.5 and 4. Yellow indicates pH values below 4. The assay was performed twice in duplicate.

(6.36 MB TIF)

Figure S2

Examples of ferritin plates as described in Table 1. SD agar was buffered using 100 mM HEPES (pH 7.4). BPS, iron chelator. Ferritin, 5 µg/ml ferritin. All plates were incubated for 3 days at 37°C under 5% CO2.

(2.68 MB TIF)

Figure S3

Ferritin binding does not require live cells or iron limitation and is UV sensitive. (A) Comparison of ferritin binding between live and dead cells (using thimerosal or UV light). (B) Cells from iron limitation medium (LIM0) or from YPD were used for the ferritin binding assay. Additionally, cells from the same YPD preculture were tested for ferritin binding with the addition of 50 µM iron chloride during the binding assay.

(0.30 MB TIF)

Figure S4

Growth of selected mutants on ferritin plates. SD agar was buffered using 100 mM HEPES (pH 7.4). BPS, iron chelator. Ferritin, 5 µg/ml ferritin. All plates were incubated for 3 days at 37°C under 5% CO2.

(1.59 MB TIF)

Figure S5

Invasion of epithelial cells by Δftr1. Aproximately 105 iron starved wild-type C. albicans cells (SC5314) or Δftr1 mutant cells were co-incubated with epithelial cells for 3 h. After fixation the samples were differentially stained and analysed under the fluorescence microscope. The experiment was performed three times in duplicate. No significant difference was observed between the wild-type strain and the Δftr1 mutant strain.

(0.30 MB TIF)

Acknowledgments

We thank A. Brown, A. Mitchell, D. Kornitzer, G. Fink, J. Ernst, K. Schröppel, Y. Wang and W. Fonzi for kindly providing C. albicans mutants used in this study and “Cancer Research Technology”, London, for kindly providing the TR146 cell line. We also thank Duncan Wilson for proof-reading of our manuscript, Anja Müller for technical assistance and Michael Mihlan for providing assistance with the flow cytometry experiment. We also would like to thank the reviewers of this manuscript for helpful suggestions.

Footnotes

The authors have declared that no competing interests exist.

RSA was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), grant BEX 1054/03-1. This work was supported by the Robert Koch Institute (RKI, Germany), the Leibniz Institute for Natural Product Research and Infection Biology–Hans Knoell Institute (HKI, Germany) and by grants R01DE017088 and R01AI019990 from the National Institutes of Health (NIH, USA).

References

1. Weinberg ED. Iron loading and disease surveillance. Emerg Infect Dis. 1999;5:346–352. [PMC free article] [PubMed]
2. Andrews SC. Bacterial iron homeostasis. FEMS Microbiol Rev. 2003;27:215–237. [PubMed]
3. Howard DH. Iron gathering by zoopathogenic fungi. FEMS Immunol Med Microbiol. 2004;40:95–100. [PubMed]
4. Ekins A. Lactoferrin receptors in gram-negative bacteria: insights into the iron acquisition process. Biometals. 2004;17:235–243. [PubMed]
5. Gerlach GF. Cloning and expression of a transferrin-binding protein from Actinobacillus pleuropneumoniae. Infect Immun. 1992;60:892–898. [PMC free article] [PubMed]
6. Jacobsen I, et al. Deletion of the ferric uptake regulator Fur impairs the in vitro growth and virulence of Actinobacillus pleuropneumoniae. Infect Immun. 2005;73:3740–3744. [PMC free article] [PubMed]
7. Knight SA. Iron acquisition from transferrin by Candida albicans depends on the reductive pathway. Infect Immun. 2005;73:5482–5492. [PMC free article] [PubMed]
8. Pettersson A. Identification of the iroA gene product of Neisseria meningitidis as a lactoferrin receptor. J Bacteriol. 1994;176:1764–1766. [PMC free article] [PubMed]
9. Ratledge C. Iron, mycobacteria and tuberculosis. Tuberculosis (Edinb) 2004;84:110–130. [PubMed]
10. Skaar EP. Iron-source preference of Staphylococcus aureus infections. Science. 2004;305:1626–1628. [PubMed]
11. Skaar EP. Bacillus anthracis IsdG, a heme-degrading monooxygenase. J Bacteriol. 2006;188:1071–1080. [PMC free article] [PubMed]
12. Stojiljkovic I, et al. The Neisseria meningitidis haemoglobin receptor: its role in iron utilization and virulence. Mol Microbiol. 1995;15:531–541. [PubMed]
13. Weissman Z. A family of Candida cell surface haem-binding proteins involved in haemin and haemoglobin-iron utilization. Mol Microbiol. 2004;53:1209–1220. [PubMed]
14. Bali PK. Receptor-induced switch in site-site cooperativity during iron release by transferrin. Biochemistry. 1992;31:3963–3967. [PubMed]
15. Klausner RD. The molecular basis of iron metabolism. Harvey Lect. 1996;92:99–112. [PubMed]
16. Rouault TA. How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003;1:e79. doi:10.1371/journal.pbio.0000079. [PMC free article] [PubMed]
17. Arosio P. Ferritin, iron homeostasis, and oxidative damage. Free Radic Biol Med. 2002;33:457–463. [PubMed]
18. Dominguez-Vera JM. Iron(III) complexation of Desferrioxamine B encapsulated in apoferritin. J Inorg Biochem. 2004;98:469–472. [PubMed]
19. Kidane TZ. Release of iron from ferritin requires lysosomal activity. Am J Physiol Cell Physiol. 2006;291:C445–455. [PubMed]
20. Radisky DC. Iron in cytosolic ferritin can be recycled through lysosomal degradation in human fibroblasts. Biochem J. 1998;336(Pt 1):201–205. [PMC free article] [PubMed]
21. Schaible UE. Iron and microbial infection. Nat Rev Microbiol. 2004;2:946–953. [PubMed]
22. Larson JA. Neisseria meningitidis accelerates ferritin degradation in host epithelial cells to yield an essential iron source. Mol Microbiol. 2004;53:807–820. [PubMed]
23. Bockmann R. Specific binding of the Listeria monocytogenes transcriptional regulator PrfA to target sequences requires additional factor(s) and is influenced by iron. Mol Microbiol. 1996;22:643–653. [PubMed]
24. McDermid JM. Iron and infection: effects of host iron status and the iron-regulatory genes haptoglobin and NRAMP1 (SLC11A1) on host-pathogen interactions in tuberculosis and HIV. Clin Sci (Lond) 2006;110:503–524. [PubMed]
25. Jung WH. Iron regulation of the major virulence factors in the AIDS-associated pathogen Cryptococcus neoformans. PLoS Biol. 2006;4:e410. doi:10.1371/journal.pbio.0040410. [PMC free article] [PubMed]
26. Jung WH, et al. Iron Source Preference and Regulation of Iron Uptake in Cryptococcus neoformans. PLoS Pathog. 2008;4:e45. doi:10.1371/journal.ppat.0040045. [PMC free article] [PubMed]
27. Boyd J. Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proc Natl Acad Sci U S A. 1990;87:5968–5972. [PMC free article] [PubMed]
28. Kragl C, et al. EstB-mediated hydrolysis of the siderophore triacetylfusarinine C optimizes iron uptake of Aspergillus fumigatus. Eukaryot Cell. 2007;6:1278–1285. [PMC free article] [PubMed]
29. Odds FC. Candida and Candidiasis. London: Bailliere Tindal; 1988.
30. Whiteway M. Morphogenesis in Candida albicans. Annu Rev Microbiol. 2007;61:529–553. [PubMed]
31. Saville SP. Use of a genetically engineered strain to evaluate the pathogenic potential of yeast cell and filamentous forms during Candida albicans systemic infection in immunodeficient mice. Infect Immun. 2008;76:97–102. [PMC free article] [PubMed]
32. Phan QT, et al. Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells. PLoS Biol. 2007;5:e64. doi:10.1371/journal.pbio.0050064. [PMC free article] [PubMed]
33. Zakikhany K, et al. In vivo transcript profiling of Candida albicans identifies a gene essential for interepithelial dissemination. Cell Microbiol. 2007;9:2938–2954. [PubMed]
34. Thewes S, et al. In vivo and ex vivo comparative transcriptional profiling of invasive and non-invasive Candida albicans isolates identifies genes associated with tissue invasion. Mol Microbiol. 2007;63:1606–1628. [PubMed]
35. Staab JF. Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans. Yeast. 1998;14:681–686. [PubMed]
36. Hoyer LL. Candida albicans ALS3 and insights into the nature of the ALS gene family. Curr Genet. 1998;33:451–459. [PubMed]
37. Sanglard D. A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence. Infect Immun. 1997;65:3539–3546. [PMC free article] [PubMed]
38. Martchenko M. Superoxide dismutases in Candida albicans: transcriptional regulation and functional characterization of the hyphal-induced SOD5 gene. Mol Biol Cell. 2004;15:456–467. [PMC free article] [PubMed]
39. Bailey DA. The Candida albicans HYR1 gene, which is activated in response to hyphal development, belongs to a gene family encoding yeast cell wall proteins. J Bacteriol. 1996;178:5353–5360. [PMC free article] [PubMed]
40. Birse CE. Cloning and characterization of ECE1, a gene expressed in association with cell elongation of the dimorphic pathogen Candida albicans. Infect Immun. 1993;61:3648–3655. [PMC free article] [PubMed]
41. Bensen ES. Transcriptional profiling in Candida albicans reveals new adaptive responses to extracellular pH and functions for Rim101p. Mol Microbiol. 2004;54:1335–1351. [PubMed]
42. Braun BR. NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction. Embo J. 2001;20:4753–4761. [PMC free article] [PubMed]
43. Murad AM, et al. NRG1 represses yeast-hypha morphogenesis and hypha-specific gene expression in Candida albicans. Embo J. 2001;20:4742–4752. [PMC free article] [PubMed]
44. Nobile CJ. Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p. Curr Biol. 2005;15:1150–1155. [PubMed]
45. Schweizer A. The TEA/ATTS transcription factor CaTec1p regulates hyphal development and virulence in Candida albicans. Mol Microbiol. 2000;38:435–445. [PubMed]
46. Stoldt VR. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi. Embo J. 1997;16:1982–1991. [PMC free article] [PubMed]
47. Lan CY, et al. Regulatory networks affected by iron availability in Candida albicans. Mol Microbiol. 2004;53:1451–1469. [PubMed]
48. Pendrak ML. Heme oxygenase in Candida albicans is regulated by hemoglobin and is necessary for metabolism of exogenous heme and hemoglobin to alpha-biliverdin. J Biol Chem. 2004;279:3426–3433. [PubMed]
49. Santos R, et al. Haemin uptake and use as an iron source by Candida albicans: role of CaHMX1-encoded haem oxygenase. Microbiology. 2003;149:579–588. [PubMed]
50. Heymann P, et al. The siderophore iron transporter of Candida albicans (Sit1p/Arn1p) mediates uptake of ferrichrome-type siderophores and is required for epithelial invasion. Infect Immun. 2002;70:5246–5255. [PMC free article] [PubMed]
51. Hu CJ. Characterization and functional analysis of the siderophore-iron transporter CaArn1p in Candida albicans. J Biol Chem. 2002;277:30598–30605. [PubMed]
52. Ismail A. Siderophore production by the pathogenic yeast, Candida albicans. Biochem Biophys Res Commun. 1985;130:885–891. [PubMed]
53. Holzberg M. Hydroxamate siderophore production by opportunistic and systemic fungal pathogens. Infect Immun. 1983;40:1134–1139. [PMC free article] [PubMed]
54. Schrettl M, et al. Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection. PLoS Pathog. 2007;3:e128. doi:10.1371/journal.ppat.0030128. [PMC free article] [PubMed]
55. Lee JH. Candida albicans can utilize siderophore during candidastasis caused by apotransferrin. Arch Pharm Res. 2006;29:249–255. [PubMed]
56. Bernier G, et al. Desketoneoenactin-siderophore conjugates for Candida: evidence of iron transport-dependent species selectivity. Antimicrob Agents Chemother. 2005;49:241–248. [PMC free article] [PubMed]
57. Lesuisse E. Siderophore uptake by Candida albicans: effect of serum treatment and comparison with Saccharomyces cerevisiae. Yeast. 2002;19:329–340. [PubMed]
58. Hammacott JE. Candida albicans CFL1 encodes a functional ferric reductase activity that can rescue a Saccharomyces cerevisiae fre1 mutant. Microbiology. 2000;146(Pt 4):869–876. [PubMed]
59. Knight SA. Reductive iron uptake by Candida albicans: role of copper, iron and the TUP1 regulator. Microbiology. 2002;148:29–40. [PubMed]
60. Kosman DJ. Molecular mechanisms of iron uptake in fungi. Mol Microbiol. 2003;47:1185–1197. [PubMed]
61. De Luca NG. Iron uptake by fungi: contrasted mechanisms with internal or external reduction. Adv Microb Physiol. 2000;43:39–74. [PubMed]
62. Eck R. A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption. Microbiology. 1999;145(Pt 9):2415–2422. [PubMed]
63. Weissman Z. Deletion of the copper transporter CaCCC2 reveals two distinct pathways for iron acquisition in Candida albicans. Mol Microbiol. 2002;44:1551–1560. [PubMed]
64. Ramanan N. A high-affinity iron permease essential for Candida albicans virulence. Science. 2000;288:1062–1064. [PubMed]
65. Fradin C. Tissue infection and site-specific gene expression in Candida albicans. Adv Appl Microbiol. 2003;53:271–290. [PubMed]
66. Schaller M. Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia. Nat Protoc. 2006;1:2767–2773. [PMC free article] [PubMed]
67. Alcain FJ. Iron reverses impermeable chelator inhibition of DNA synthesis in CCl 39 cells. Proc Natl Acad Sci U S A. 1994;91:7903–7906. [PMC free article] [PubMed]
68. Park H, et al. Role of the fungal Ras-protein kinase A pathway in governing epithelial cell interactions during oropharyngeal candidiasis. Cell Microbiol. 2005;7:499–510. [PubMed]
69. Lapathitis G. Different sources of acidity in glucose-elicited extracellular acidification in the yeast Saccharomyces cerevisiae. Biochem Mol Biol Int. 1998;46:973–978. [PubMed]
70. Kotyk A. Glucose- and K(+)-induced acidification in different yeast species. Folia Microbiol (Praha) 1999;44:295–298. [PubMed]
71. Torres VJ. Staphylococcus aureus IsdB is a hemoglobin receptor required for heme iron utilization. J Bacteriol. 2006;188:8421–8429. [PMC free article] [PubMed]
72. Feng Q. Ras signaling is required for serum-induced hyphal differentiation in Candida albicans. J Bacteriol. 1999;181:6339–6346. [PMC free article] [PubMed]
73. Lo HJ, et al. Nonfilamentous C. albicans mutants are avirulent. Cell. 1997;90:939–949. [PubMed]
74. Zheng X. Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis. Embo J. 2004;23:1845–1856. [PMC free article] [PubMed]
75. Hoyer LL. The ALS gene family of Candida albicans. Trends Microbiol. 2001;9:176–180. [PubMed]
76. Nobile CJ, et al. Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo. PLoS Pathog. 2006;2:e63. doi:10.1371/journal.ppat.0020063. [PMC free article] [PubMed]
77. Sheppard DC, et al. Functional and structural diversity in the Als protein family of Candida albicans. J Biol Chem. 2004;279:30480–30489. [PubMed]
78. Doherty CP. Host-pathogen interactions: the role of iron. J Nutr. 2007;137:1341–1344. [PubMed]
79. Boelaert JR. The effect of the host's iron status on tuberculosis. J Infect Dis. 2007;195:1745–1753. [PubMed]
80. Abe F. Experimental candidiasis in iron overload. Mycopathologia. 1985;89:59–63. [PubMed]
81. Fratti RA. Endothelial cell injury caused by Candida albicans is dependent on iron. Infect Immun. 1998;66:191–196. [PMC free article] [PubMed]
82. Niewerth M, et al. Ciclopirox olamine treatment affects the expression pattern of Candida albicans genes encoding virulence factors, iron metabolism proteins, and drug resistance factors. Antimicrob Agents Chemother. 2003;47:1805–1817. [PMC free article] [PubMed]
83. Sigle HC, et al. Oxygen accessibility and iron levels are critical factors for the antifungal action of ciclopirox against Candida albicans. J Antimicrob Chemother. 2005;55:663–673. [PubMed]
84. Lee RE. Genome-wide expression profiling of the response to ciclopirox olamine in Candida albicans. J Antimicrob Chemother. 2005;55:655–662. [PubMed]
85. Sikkema DJ. Outer membrane peptides of Yersinia pestis mediating siderophore-independent assimilation of iron. Biol Met. 1989;2:174–184. [PubMed]
86. Gobin J. Exochelins of Mycobacterium tuberculosis remove iron from human iron-binding proteins and donate iron to mycobactins in the M. tuberculosis cell wall. J Exp Med. 1996;183:1527–1532. [PMC free article] [PubMed]
87. Jin B, et al. Iron acquisition systems for ferric hydroxamates, haemin and haemoglobin in Listeria monocytogenes. Mol Microbiol. 2006;59:1185–1198. [PubMed]
88. Whitby PW, et al. Burkholderia cenocepacia utilizes ferritin as an iron source. J Med Microbiol. 2006;55:661–668. [PubMed]
89. Naglik J. Candida albicans proteinases and host/pathogen interactions. Cell Microbiol. 2004;6:915–926. [PubMed]
90. Ruchel R. Properties of a purified proteinase from the yeast Candida albicans. Biochim Biophys Acta. 1981;659:99–113. [PubMed]
91. Friedman DB, et al. Staphylococcus aureus redirects central metabolism to increase iron availability. PLoS Pathog. 2006;2:e87. doi:10.1371/journal.ppat.0020087. [PMC free article] [PubMed]
92. Joo MS. Rapid reduction of iron in horse spleen ferritin by thioglycolic acid measured by dispersive X-ray absorption spectroscopy. Biol Met. 1990;3:171–175. [PubMed]
93. Laulhere JP. Dynamic equilibria in iron uptake and release by ferritin. Biometals. 1996;9:303–309. [PubMed]
94. Galvez N. Release of iron from ferritin by aceto- and benzohydroxamic acids. Inorg Chem. 2005;44:2706–2709. [PubMed]
95. Genco CA. Emerging strategies in microbial haem capture. Mol Microbiol. 2001;39:1–11. [PubMed]
96. Voss P, et al. Irradiation of GAPDH: a model for environmentally induced protein damage. Biol Chem. 2007;388:583–592. [PubMed]
97. Zhao X, et al. ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparisons between Als3p and Als1p. Microbiology. 2004;150:2415–2428. [PubMed]
98. Biswas S. Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans. Microbiol Mol Biol Rev. 2007;71:348–376. [PMC free article] [PubMed]
99. Sundstrom P. Adhesion in Candida spp. Cell Microbiol. 2002;4:461–469. [PubMed]
100. Davis D. RIM101-dependent and-independent pathways govern pH responses in Candida albicans. Mol Cell Biol. 2000;20:971–978. [PMC free article] [PubMed]
101. Soll DR. Filament ring formation in the dimorphic yeast Candida albicans. J Cell Biol. 1983;96:486–493. [PMC free article] [PubMed]
102. Sosinska GJ, et al. Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions. Microbiology. 2008;154:510–520. [PubMed]
103. White SJ, et al. Self-regulation of Candida albicans population size during GI colonization. PLoS Pathog. 2007;3:e184. doi:10.1371/journal.ppat.0030184. [PMC free article] [PubMed]
104. Hoyer LL. Discovering the secrets of the Candida albicans agglutinin-like sequence (ALS) gene family–a sticky pursuit. Med Mycol. 2008;46:1–15. [PMC free article] [PubMed]
105. Eide D. Increased dosage of a transcriptional activator gene enhances iron-limited growth of Saccharomyces cerevisiae. J Gen Microbiol. 1992;138:347–354. [PubMed]
106. Rupniak HT, et al. Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. J Natl Cancer Inst. 1985;75:621–635. [PubMed]
107. Fuchs S, et al. Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 2003;311:31–45. [PubMed]
108. Murad AM. CIp10, an efficient and convenient integrating vector for Candida albicans. Yeast. 2000;16:325–327. [PubMed]
109. Fradin C, et al. Granulocytes govern the transcriptional response, morphology and proliferation of Candida albicans in human blood. Mol Microbiol. 2005;56:397–415. [PubMed]
110. Gillum AM. Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet. 1984;198:179–182. [PubMed]
111. Fonzi WA. Isogenic strain construction and gene mapping in Candida albicans. Genetics. 1993;134:717–728. [PMC free article] [PubMed]
112. Kretschmar M, et al. Individual acid aspartic proteinases (Saps) 1–6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice. Microb Pathog. 2002;32:61–70. [PubMed]

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