Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. 1991 Jul; 59(7): 2311–2315.
PMCID: PMC258012

Association of carboxylesterase B electrophoretic pattern with presence and expression of urovirulence factor determinants and antimicrobial resistance among strains of Escherichia coli that cause urosepsis.


We determined the carboxylesterase B electrophoretic profiles of 74 blood isolates of Escherichia coli from patients with urosepsis. Most strains (64%) exhibited the B2 electrophoretic pattern. P fimbrial and hemolysin genetic determinants were present and expressed significantly more often among strains with the B2 than with the B1 electrophoretic pattern. In contrast, aerobactin determinants were significantly more prevalent and more commonly expressed among the B1 strains; this difference was attributable to the presence of plasmid-encoded aerobactin in one-third of the B1 strains (P = 0.02, B1 versus B2). The prevalence and extent of antimicrobial resistance was significantly greater among the B1 strains, and the B1 electrophoretic pattern was more often found in isolates from patients with urinary tract abnormalities. We conclude that the carboxylesterase B electrophoretic pattern differentiates two groups of E. coli isolates from patients with urosepsis: strains with the B1 electrophoretic pattern are associated with urologically impaired hosts, characteristically lack P fimbrial and hemolysin determinants, and often carry a plasmid-encoded aerobactin system (possibly on multiple antimicrobial resistance plasmids), whereas B2 strains more commonly invade noncompromised hosts, express P fimbriae and hemolysin, carry chromosomal aerobactin determinants, and lack antimicrobial resistance.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1016K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Achtman M, Mercer A, Kusecek B, Pohl A, Heuzenroeder M, Aaronson W, Sutton A, Silver RP. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983 Jan;39(1):315–335. [PMC free article] [PubMed]
  • Brenner DJ, Fanning GR, Johnson KE, Citarella RV, Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. [PMC free article] [PubMed]
  • Carbonetti NH, Williams PH. A cluster of five genes specifying the aerobactin iron uptake system of plasmid ColV-K30. Infect Immun. 1984 Oct;46(1):7–12. [PMC free article] [PubMed]
  • Caugant DA, Levin BR, Orskov I, Orskov F, Svanborg Eden C, Selander RK. Genetic diversity in relation to serotype in Escherichia coli. Infect Immun. 1985 Aug;49(2):407–413. [PMC free article] [PubMed]
  • Goullet P, Picard B. Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources. J Gen Microbiol. 1986 Jul;132(7):1843–1851. [PubMed]
  • Goullet P, Picard B. Highly pathogenic strains of Escherichia coli revealed by the distinct electrophoretic patterns of carboxylesterase B. J Gen Microbiol. 1986 Jul;132(7):1853–1858. [PubMed]
  • Goullet P, Picard B. Comparative electrophoretic polymorphism of esterases and other enzymes in Escherichia coli. J Gen Microbiol. 1989 Jan;135(1):135–143. [PubMed]
  • Goullet P, Picard B. Electrophoretic type B2 of carboxylesterase B for characterisation of highly pathogenic Escherichia coli strains from extra-intestinal infections. J Med Microbiol. 1990 Sep;33(1):11–16. [PubMed]
  • Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991 Jan;4(1):80–128. [PMC free article] [PubMed]
  • Johnson JR, Moseley SL, Roberts PL, Stamm WE. Aerobactin and other virulence factor genes among strains of Escherichia coli causing urosepsis: association with patient characteristics. Infect Immun. 1988 Feb;56(2):405–412. [PMC free article] [PubMed]
  • Johnson JR, Roberts PL, Stamm WE. P fimbriae and other virulence factors in Escherichia coli urosepsis: association with patients' characteristics. J Infect Dis. 1987 Jul;156(1):225–229. [PubMed]
  • Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. [PMC free article] [PubMed]
  • Maas R. An improved colony hybridization method with significantly increased sensitivity for detection of single genes. Plasmid. 1983 Nov;10(3):296–298. [PubMed]
  • Picard B, Goullet P. Correlation between electrophoretic types B1 and B2 of carboxylesterase B and host-dependent factors in Escherichia coli septicaemia. Epidemiol Infect. 1988 Feb;100(1):51–61. [PMC free article] [PubMed]
  • Picard B, Goullet P. Correlation between electrophoretic types B1 and B2 of carboxylesterase B and sex of patients in Escherichia coli urinary tract infections. Epidemiol Infect. 1989 Aug;103(1):97–103. [PMC free article] [PubMed]
  • Orskov I, Williams PH, Svanborg Edén C, Orskov F. Assessment of biological and colony hybridization assays for detection of the aerobactin system in Escherichia coli from urinary tract infections. Med Microbiol Immunol. 1989;178(3):143–148. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...