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Infect Immun. Jun 1991; 59(6): 1875–1880.
PMCID: PMC257936

Helicobacter mustelae-induced gastritis and elevated gastric pH in the ferret (Mustela putorius furo).

Abstract

Helicobacter mustelae has been cultured from the stomachs of ferrets with chronic gastritis; the lesions in the stomach have many of the same histological features seen in H. pylori gastritis in humans. To determine whether H. mustelae-negative ferrets with normal gastric mucosa were susceptible to colonization and whether gastritis developed after infection, four H. mustelae-negative ferrets treated with cimetidine were inoculated orally on two successive days with 3 ml (1.5 x 10(8) CFU) of H. mustelae; eight age-matched H. mustelae-negative ferrets served as controls. All four ferrets became colonized; H. mustelae persisted through week 24 of the study, as determined by positive gastric culture, tissue urease, and Warthin-Starry staining of gastric tissue. Superficial gastritis developed in the oxyntic gastric mucosa, and a full-thickness gastritis, composed primarily of lymphocytes and plasma cells plus small numbers of neutrophils and eosinophils, was present in the antrum. The inflammation was accompanied by an elevation of immunoglobulin G antibody to H. mustelae. At 4 weeks post-inoculation, the four infected (experimental) ferrets developed an elevated gastric pH (4.0 to 5.2) for 2 weeks. The eight control ferrets did not have gastritis; H. mustelae could not be demonstrated in gastric tissue via culture, nor was there an immune response to the bacteria. In ferrets, H. mustelae readily colonizes the stomach and produces a gastritis, a significant immune response, and, like H. pylori infection in humans, a transient elevated gastric pH after Helicobacter infection.

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  • Barthel JS, Westblom TU, Havey AD, Gonzalez F, Everett ED. Gastritis and Campylobacter pylori in healthy, asymptomatic volunteers. Arch Intern Med. 1988 May;148(5):1149–1151. [PubMed]
  • Baskerville A, Newell DG. Naturally occurring chronic gastritis and C pylori infection in the rhesus monkey: a potential model for gastritis in man. Gut. 1988 Apr;29(4):465–472. [PMC free article] [PubMed]
  • Cantorna MT, Balish E. Inability of human clinical strains of Helicobacter pylori to colonize the alimentary tract of germfree rodents. Can J Microbiol. 1990 Apr;36(4):237–241. [PubMed]
  • Cave DR, Vargas M. Effect of a Campylobacter pylori protein on acid secretion by parietal cells. Lancet. 1989 Jul 22;2(8656):187–189. [PubMed]
  • Chen XG, Correa P, Offerhaus J, Rodriguez E, Janney F, Hoffmann E, Fox J, Hunter F, Diavolitsis S. Ultrastructure of the gastric mucosa harboring Campylobacter-like organisms. Am J Clin Pathol. 1986 Nov;86(5):575–582. [PubMed]
  • Czinn SJ, Dahms BB, Jacobs GH, Kaplan B, Rothstein FC. Campylobacter-like organisms in association with symptomatic gastritis in children. J Pediatr. 1986 Jul;109(1):80–83. [PubMed]
  • Engstrand L, Gustavsson S, Jörgensen A, Schwan A, Scheynius A. Inoculation of barrier-born pigs with Helicobacter pylori: a useful animal model for gastritis type B. Infect Immun. 1990 Jun;58(6):1763–1768. [PMC free article] [PubMed]
  • Fox JG, Cabot EB, Taylor NS, Laraway R. Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets. Infect Immun. 1988 Nov;56(11):2994–2996. [PMC free article] [PubMed]
  • Fox JG, Correa P, Taylor NS, Lee A, Otto G, Murphy JC, Rose R. Helicobacter mustelae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans. Gastroenterology. 1990 Aug;99(2):352–361. [PubMed]
  • Fox JG, Correa P, Taylor NS, Zavala D, Fontham E, Janney F, Rodriguez E, Hunter F, Diavolitsis S. Campylobacter pylori-associated gastritis and immune response in a population at increased risk of gastric carcinoma. Am J Gastroenterol. 1989 Jul;84(7):775–781. [PubMed]
  • Fox JG, Edrise BM, Cabot EB, Beaucage C, Murphy JC, Prostak KS. Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res. 1986 Feb;47(2):236–239. [PubMed]
  • Fox JG, Lee A. Gastric campylobacter-like organisms: their role in gastric disease of laboratory animals. Lab Anim Sci. 1989 Nov;39(6):543–553. [PubMed]
  • Fox JG, Lee A, Otto G, Taylor NS, Murphy JC. Helicobacter felis gastritis in gnotobiotic rats: an animal model of Helicobacter pylori gastritis. Infect Immun. 1991 Mar;59(3):785–791. [PMC free article] [PubMed]
  • Graham DY, Alpert LC, Smith JL, Yoshimura HH. Iatrogenic Campylobacter pylori infection is a cause of epidemic achlorhydria. Am J Gastroenterol. 1988 Sep;83(9):974–980. [PubMed]
  • Hazell SL, Borody TJ, Gal A, Lee A. Campylobacter pyloridis gastritis I: Detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol. 1987 Apr;82(4):292–296. [PubMed]
  • Henry GA, Long PH, Burns JL, Charbonneau DL. Gastric spirillosis in beagles. Am J Vet Res. 1987 May;48(5):831–836. [PubMed]
  • Krakowka S, Morgan DR, Kraft WG, Leunk RD. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun. 1987 Nov;55(11):2789–2796. [PMC free article] [PubMed]
  • Lee A, Fox JG, Otto G, Murphy J. A small animal model of human Helicobacter pylori active chronic gastritis. Gastroenterology. 1990 Nov;99(5):1315–1323. [PubMed]
  • Lee A, Hazell SL, O'Rourke J, Kouprach S. Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun. 1988 Nov;56(11):2843–2850. [PMC free article] [PubMed]
  • Lockard VG, Boler RK. Ultrastructure of a spiraled microorganism in the gastric mucosa of dogs. Am J Vet Res. 1970 Aug;31(8):1453–1462. [PubMed]
  • Marshall BJ, Armstrong JA, McGechie DB, Glancy RJ. Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med J Aust. 1985 Apr 15;142(8):436–439. [PubMed]
  • Marshall BJ, Barrett LJ, Prakash C, McCallum RW, Guerrant RL. Urea protects Helicobacter (Campylobacter) pylori from the bactericidal effect of acid. Gastroenterology. 1990 Sep;99(3):697–702. [PubMed]
  • Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. [PubMed]
  • McNulty CA, Dent JC, Curry A, Uff JS, Ford GA, Gear MW, Wilkinson SP. New spiral bacterium in gastric mucosa. J Clin Pathol. 1989 Jun;42(6):585–591. [PMC free article] [PubMed]
  • Morris A, Nicholson G. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol. 1987 Mar;82(3):192–199. [PubMed]
  • Otto G, Fox JG, Wu PY, Taylor NS. Eradication of Helicobacter mustelae from the ferret stomach: an animal model of Helicobacter (Campylobacter) pylori chemotherapy. Antimicrob Agents Chemother. 1990 Jun;34(6):1232–1236. [PMC free article] [PubMed]
  • Radin MJ, Eaton KA, Krakowka S, Morgan DR, Lee A, Otto G, Fox J. Helicobacter pylori gastric infection in gnotobiotic beagle dogs. Infect Immun. 1990 Aug;58(8):2606–2612. [PMC free article] [PubMed]
  • Ramsey EJ, Carey KV, Peterson WL, Jackson JJ, Murphy FK, Read NW, Taylor KB, Trier JS, Fordtran JS. Epidemic gastritis with hypochlorhydria. Gastroenterology. 1979 Jun;76(6):1449–1457. [PubMed]
  • Rauws EA, Tytgat GN. Cure of duodenal ulcer associated with eradication of Helicobacter pylori. Lancet. 1990 May 26;335(8700):1233–1235. [PubMed]
  • Sato T, Takeuchi A. Infection by spirilla in the stomach of the rhesus monkey. Vet Pathol Suppl. 1982 Sep;19 (Suppl 7):17–25. [PubMed]

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