Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Apr 1991; 59(4): 1558–1563.
PMCID: PMC257876

T-cell proliferative response to antigens secreted by Mycobacterium tuberculosis.

Abstract

An infection model of human tuberculosis was established with C57BL/6J mice. The lymphocyte proliferative responses to antigens from Mycobacterium tuberculosis were investigated during the course of infection and compared with results obtained with a group of mice immunized with large amounts of killed bacteria. The two groups responded similarly to a number of mycobacterial antigens, but marked differences in responses against secreted antigens were found; only infected mice responded vigorously to these. The responding lymphocyte subpopulation was made up of L3T4+ T lymphocytes under restriction of the Ia molecule.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abou-Zeid C, Ratliff TL, Wiker HG, Harboe M, Bennedsen J, Rook GA. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG. Infect Immun. 1988 Dec;56(12):3046–3051. [PMC free article] [PubMed]
  • Abou-Zeid C, Smith I, Grange JM, Ratliff TL, Steele J, Rook GA. The secreted antigens of Mycobacterium tuberculosis and their relationship to those recognized by the available antibodies. J Gen Microbiol. 1988 Feb;134(2):531–538. [PubMed]
  • Andersen AB, Yuan ZL, Hasløv K, Vergmann B, Bennedsen J. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol. 1986 Mar;23(3):446–451. [PMC free article] [PubMed]
  • Barnes PF, Mehra V, Hirschfield GR, Fong SJ, Abou-Zeid C, Rook GA, Hunter SW, Brennan PJ, Modlin RL. Characterization of T cell antigens associated with the cell wall protein-peptidoglycan complex of Mycobacterium tuberculosis. J Immunol. 1989 Oct 15;143(8):2656–2662. [PubMed]
  • Borremans M, de Wit L, Volckaert G, Ooms J, de Bruyn J, Huygen K, van Vooren JP, Stelandre M, Verhofstadt R, Content J. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis. Infect Immun. 1989 Oct;57(10):3123–3130. [PMC free article] [PubMed]
  • Brett SJ, Lamb JR, Cox JH, Rothbard JB, Mehlert A, Ivanyi J. Differential pattern of T cell recognition of the 65-kDa mycobacterial antigen following immunization with the whole protein or peptides. Eur J Immunol. 1989 Jul;19(7):1303–1310. [PubMed]
  • Collins FM, Lamb JR, Young DB. Biological activity of protein antigens isolated from Mycobacterium tuberculosis culture filtrate. Infect Immun. 1988 May;56(5):1260–1266. [PMC free article] [PubMed]
  • Czuprynski CJ, Brown JF, Young KM, Cooley AJ. Administration of purified anti-L3T4 monoclonal antibody impairs the resistance of mice to Listeria monocytogenes infection. Infect Immun. 1989 Jan;57(1):100–109. [PMC free article] [PubMed]
  • Duquesne V, Auriault C, Darcy F, Decavel JP, Capron A. Protection of nude rats against Toxoplasma infection by excreted-secreted antigen-specific helper T cells. Infect Immun. 1990 Jul;58(7):2120–2126. [PMC free article] [PubMed]
  • David HL, Lévy-Frébault V, Thorel MF. Characterization of distinct layers of the Mycobacterium avium envelope in respect of their composition by fatty acids, proteins, oligosaccharides and antigens. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Apr;268(2):193–208. [PubMed]
  • Huygen K, Van Vooren JP, Turneer M, Bosmans R, Dierckx P, De Bruyn J. Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis. Scand J Immunol. 1988 Feb;27(2):187–194. [PubMed]
  • Julius MH, Simpson E, Herzenberg LA. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Leveton C, Barnass S, Champion B, Lucas S, De Souza B, Nicol M, Banerjee D, Rook G. T-cell-mediated protection of mice against virulent Mycobacterium tuberculosis. Infect Immun. 1989 Feb;57(2):390–395. [PMC free article] [PubMed]
  • Mackaness GB. The relationship of delayed hypersensitivity to acquired cellular resistance. Br Med Bull. 1967 Jan;23(1):52–54. [PubMed]
  • Magee DM, Wing EJ. Cloned L3T4+ T lymphocytes protect mice against Listeria monocytogenes by secreting IFN-gamma. J Immunol. 1988 Nov 1;141(9):3203–3207. [PubMed]
  • Matsuo K, Yamaguchi R, Yamazaki A, Tasaka H, Yamada T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J Bacteriol. 1988 Sep;170(9):3847–3854. [PMC free article] [PubMed]
  • Morrissey JH. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. [PubMed]
  • Müller I, Cobbold SP, Waldmann H, Kaufmann SH. Impaired resistance to Mycobacterium tuberculosis infection after selective in vivo depletion of L3T4+ and Lyt-2+ T cells. Infect Immun. 1987 Sep;55(9):2037–2041. [PMC free article] [PubMed]
  • Nagai S, Matsumoto J, Nagasuga T. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG. Infect Immun. 1981 Mar;31(3):1152–1160. [PMC free article] [PubMed]
  • Orme IM. The kinetics of emergence and loss of mediator T lymphocytes acquired in response to infection with Mycobacterium tuberculosis. J Immunol. 1987 Jan 1;138(1):293–298. [PubMed]
  • Orme IM. Induction of nonspecific acquired resistance and delayed-type hypersensitivity, but not specific acquired resistance in mice inoculated with killed mycobacterial vaccines. Infect Immun. 1988 Dec;56(12):3310–3312. [PMC free article] [PubMed]
  • Orme IM. Characteristics and specificity of acquired immunologic memory to Mycobacterium tuberculosis infection. J Immunol. 1988 May 15;140(10):3589–3593. [PubMed]
  • Rook GA, Steele J, Barnass S, Mace J, Stanford JL. Responsiveness to live M. tuberculosis, and common antigens, of sonicate-stimulated T cell lines from normal donors. Clin Exp Immunol. 1986 Jan;63(1):105–110. [PMC free article] [PubMed]
  • Silberstein DS, Despommier DD. Antigens from trichinella spiralis that induce a protective response in the mouse. J Immunol. 1984 Feb;132(2):898–904. [PubMed]
  • Worsaae A, Ljungqvist L, Hasløv K, Heron I, Bennedsen J. Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs. Infect Immun. 1987 Dec;55(12):2922–2927. [PMC free article] [PubMed]
  • Worsaae A, Ljungqvist L, Heron I. Monoclonal antibodies produced in BALB.B10 mice define new antigenic determinants in culture filtrate preparations of Mycobacterium tuberculosis. J Clin Microbiol. 1988 Dec;26(12):2608–2614. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...