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J Virol. May 1982; 42(2): 612–620.
PMCID: PMC256886

Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen.

Abstract

Several recently isolated monoclonal antibodies which reacted with simian virus 40 T antigens also reacted with proteins found in uninfected and untransformed cells. The proteins were different from each other, PAb419 reacting with a 35,000-molecular-weight protein, PAb427 reacting with a 75,000-molecular-weight phosphoprotein, PAb405 reacting with a 150,000-molecular-weight phosphoprotein, and PAb204 reacting with a 68,000-molecular-weight protein. It is suggested that although some of these cross-reactions may be fortuitous, they may, as an alternative, reflect similarities of shape and perhaps function between domains of the viral T antigen and the relevant host proteins.

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  • Atassi MZ. Antigenic structure of myoglobin: the complete immunochemical anatomy of a protein and conclusions relating to antigenic structures of proteins. Immunochemistry. 1975 May;12(5):423–438. [PubMed]
  • Atassi MZ. Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of "surface-simulation" synthesis--a powerful new concept for protein binding sites. Immunochemistry. 1978 Dec;15(12):909–936. [PubMed]
  • AUERSPERG N. LONG-TERM CULTIVATION OF HYPODIPLOID HUMAN TUMOR CELLS. J Natl Cancer Inst. 1964 Jan;32:135–163. [PubMed]
  • Berezney R, Coffey DS. Identification of a nuclear protein matrix. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410–1417. [PubMed]
  • Breschkin AM, Ahern J, White DO. Antigenic determinants of influenza virus hemagglutinin. VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies. Virology. 1981 Aug;113(1):130–140. [PubMed]
  • Buckler-White AJ, Humphrey GW, Pigiet V. Association of polyoma T antigen and DNA with the nuclear matrix from lytically infected 3T6 cells. Cell. 1980 Nov;22(1 Pt 1):37–46. [PubMed]
  • Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed]
  • Dulbecco R, Unger M, Bologna M, Battifora H, Syka P, Okada S. Cross-reactivity between Thy-1 and a component of intermediate filaments demonstrated using a monoclonal antibody. Nature. 1981 Aug 20;292(5825):772–774. [PubMed]
  • Galanti N, Jonak GJ, Soprano KJ, Floros J, Kaczmarek L, Weissman S, Reddy VB, Tilghman SM, Baserga R. Characterization and biological activity of cloned simian virus 40 DNA fragments. J Biol Chem. 1981 Jun 25;256(12):6469–6474. [PubMed]
  • Graessmann M, Graessman A. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication. Proc Natl Acad Sci U S A. 1976 Feb;73(2):366–370. [PMC free article] [PubMed]
  • Graessmann A, Graessmann M, Mueller C. Regulatory function of simian virus 40 DNA replication for late viral gene expression. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4831–4834. [PMC free article] [PubMed]
  • Gurney EG, Harrison RO, Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980 Jun;34(3):752–763. [PMC free article] [PubMed]
  • Hackett AJ, Smith HS, Springer EL, Owens RB, Nelson-Rees WA, Riggs JL, Gardner MB. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines. J Natl Cancer Inst. 1977 Jun;58(6):1795–1806. [PubMed]
  • Harlow E, Crawford LV, Pim DC, Williamson NM. Monoclonal antibodies specific for simian virus 40 tumor antigens. J Virol. 1981 Sep;39(3):861–869. [PMC free article] [PubMed]
  • Hassell JA, Lukanidin E, Fey G, Sambrook J. The structure and expression of two defective adenovirus 2/simian virus 40 hybrids. J Mol Biol. 1978 Apr 5;120(2):209–247. [PubMed]
  • Kessler SW. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed]
  • Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. [PubMed]
  • Lane DP, Crawford LV. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979 Mar 15;278(5701):261–263. [PubMed]
  • Lane DP, Crawford LV. The complex between simian virus 40 T antigen and a specific host protein. Proc R Soc Lond B Biol Sci. 1980 Nov 19;210(1180):451–463. [PubMed]
  • Lane DP, Hoeffler WK. SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000. Nature. 1980 Nov 13;288(5787):167–170. [PubMed]
  • Lubeck MD, Gerhard W. Topological mapping antigenic sites on the influenza A/PR/8/34 virus hemagglutinin using monoclonal antibodies. Virology. 1981 Aug;113(1):64–72. [PubMed]
  • Martinis J, Croce CM. Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40. Proc Natl Acad Sci U S A. 1978 May;75(5):2320–2323. [PMC free article] [PubMed]
  • Pillemer E, Weissman IL. A monoclonal antibody that detects a V kappa-TEPC15 idiotypic determinant cross-reactive with a Thy-1 determinant. J Exp Med. 1981 May 1;153(5):1068–1079. [PMC free article] [PubMed]
  • Takemoto KK, Kirschstein RL, Habel K. Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity. J Bacteriol. 1966 Oct;92(4):990–994. [PMC free article] [PubMed]
  • Thummel CS, Burgess TL, Tjian R. Properties of simian virus 40 small t antigen overproduced in bacteria. J Virol. 1981 Feb;37(2):683–697. [PMC free article] [PubMed]
  • Tjian R. The binding site on SV40 DNA for a T antigen-related protein. Cell. 1978 Jan;13(1):165–179. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • WALEN KH, BROWN SW. Chromosomes in a marsupial (Potorous tridactylis) tissue culture. Nature. 1962 Apr 28;194:406–406. [PubMed]

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