• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Dec 1981; 40(3): 932–935.
PMCID: PMC256705

Molecular cloning of viral DNA from human genital warts.


The DNA of human papilloma virus type 6 (HPV 6) has been cloned in Escherichia coli K-12 by using pBR322 as vector. The DNA was cloned at the BamHI and EcoRI cleavage sites. This DNA was mapped by employing further restriction endonucleases and by terminal labeling. No major differences were noted as compared to HPV 6 DNA originating directly from a genital wart. The existence of at least two DNA subtypes (HPV 6a and 6b) became apparent.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (595K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Danos O, Katinka M, Yaniv M. Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1a DNA. Eur J Biochem. 1980 Aug;109(2):457–461. [PubMed]
  • Delap RD, Friedman-klen A, Rush MG. The absence of human papilloma viral DNA sequences in condylomata acuminata. Virology. 1976 Oct 1;74(1):268–272. [PubMed]
  • Gissmann L, Pfister H, Zur Hausen H. Human papilloma viruses (HPV): characterization of four different isolates. Virology. 1977 Feb;76(2):569–580. [PubMed]
  • Gissmann L, zur Hausen H. Partial characterization of viral DNA from human genital warts (Condylomata acuminata). Int J Cancer. 1980 May 15;25(5):605–609. [PubMed]
  • Heilman CA, Law MF, Israel MA, Howley PM. Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J Virol. 1980 Nov;36(2):395–407. [PMC free article] [PubMed]
  • Koller B, Delius H, Bünemann H, Müller W. The isolation of DNA from agarose gels by electrophoretic elution onto malachite green-polyacrylamide columns. Gene. 1978 Nov;4(3):227–239. [PubMed]
  • Kruczek I, Schwarz E, zur Hausen H. Mutants of adenovirus type 12 after adaptation to growth in tumor cell lines. II. Reproducible acquisition of additional sequences after adaptation to a human cervical cancer line. Int J Cancer. 1981 Feb 15;27(2):139–143. [PubMed]
  • Krzyzek RA, Watts SL, Anderson DL, Faras AJ, Pass F. Anogenital warts contain several distinct species of human papillomavirus. J Virol. 1980 Oct;36(1):236–244. [PMC free article] [PubMed]
  • Orth G, Favre M, Croissant O. Characterization of a new type of human papillomavirus that causes skin warts. J Virol. 1977 Oct;24(1):108–120. [PMC free article] [PubMed]
  • Orth G, Favre M, Jablonska S, Brylak K, Croissant O. Viral sequences related to a human skin papillomavirus in genital warts. Nature. 1978 Sep 28;275(5678):334–336. [PubMed]
  • Pfister H, Nürnberger F, Gissmann L, zur Hausen H. Characterization of a human papillomavirus from epidermodysplasia verruciformis lesions of a patient from Upper-volta. Int J Cancer. 1981 May 15;27(5):645–650. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Sutcliffe JG. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737–3741. [PMC free article] [PubMed]
  • zur Hausen H. Oncogenic Herpes viruses. Biochim Biophys Acta. 1975 Mar 20;417(1):25–53. [PubMed]
  • zur Hausen H. Human papillomaviruses and their possible role in squamous cell carcinomas. Curr Top Microbiol Immunol. 1977;78:1–30. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...