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J Virol. Dec 1982; 44(3): 993–1002.
PMCID: PMC256359

Lytic action of cloned phi X174 gene E.

Abstract

The phi X174 lysis gene E was placed under control of the lac promoter by cloning into the multicopy plasmid pBH20. Other phi X174 gene sequences were removed by nuclease digestion. Expression of gene E was shown to be necessary and sufficient to produce lysis phenomena exhibited by infection with intact phage. Lysis, its inhibition by MgSO4 and spermine, its progression through a spheroplasting stage, and its dependence on an early chloramphenicol-sensitive step were reproduced in clones induced for expression of the E gene product. Escherichia coli clones carrying the E gene not under lac control, and clones under lac control but only minimally induced for gene E expression, exhibited morphological aberrations consistent with the view that the mechanism by which gene E mediates cell lysis is related to host cell division processes.

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Selected References

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  • Henrich B, Lubitz W, Plapp R. Lysis of Escherichia coli by induction of cloned phi X174 genes. Mol Gen Genet. 1982;185(3):493–497. [PubMed]
  • Aoyama A, Hamatake RK, Hayashi M. Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285–7289. [PMC free article] [PubMed]
  • Barrell BG, Air GM, Hutchison CA., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. [PubMed]
  • Bieńkowska-Szewczyk K, Taylor A. Murein transglycosylase from phage lambda lysate. Purification and properties. Biochim Biophys Acta. 1980 Oct;615(2):489–496. [PubMed]
  • Bradley DE. Spheroplast formation in cells of Escherichia coli infected with a phi-X 174 type bacteriophage. J Gen Virol. 1968 Jul;3(1):141–142. [PubMed]
  • Bradley DE, Dewar CA, Robertson D. Structural changes in Escherichia coli infected with a phi X174 type bacteriophage. J Gen Virol. 1969 Jul;5(1):113–121. [PubMed]
  • Breeden L, Yarus M, Cline S. A cloned suppressor tRNA gene relaxes stringent control. Mol Gen Genet. 1980;179(1):125–133. [PubMed]
  • FUJIMURA R, KAESBERG P. The adsorption of bacteriophage phi-X174 to its host. Biophys J. 1962 Nov;2:433–449. [PMC free article] [PubMed]
  • Garrett J, Fusselman R, Hise J, Chiou L, Smith-Grillo D, Schulz J, Young R. Cell lysis by induction of cloned lambda lysis genes. Mol Gen Genet. 1981;182(2):326–331. [PubMed]
  • Groman NB, Suzuki G. Effect of spermine on lysis and reproduction by bacteriophages phi-X174, lambda, and f2. J Bacteriol. 1966 Dec;92(6):1735–1740. [PMC free article] [PubMed]
  • Gschwender HH, Hofschneider PH. Lysis inhibition of phi-X174-, M12-, and Q-beta-infected Escherichia coli bacteria by magnesium ions. Biochim Biophys Acta. 1969 Oct 22;190(2):454–459. [PubMed]
  • Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. [PubMed]
  • HUTCHISON CA, SINSHEIMER RL. KINETICS OF BACTERIOPHAGE RELEASE BY SINGLE CELLS OF PHI X174-INFECTED E. COLI. J Mol Biol. 1963 Aug;7:206–208. [PubMed]
  • Hutchison CA, 3rd, Sinsheimer RL. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. [PubMed]
  • Itakura K, Hirose T, Crea R, Riggs AD, Heyneker HL, Bolivar F, Boyer HW. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science. 1977 Dec 9;198(4321):1056–1063. [PubMed]
  • Josslin R. The lysis mechanism of phage T4: mutants affecting lysis. Virology. 1970 Mar;40(3):719–726. [PubMed]
  • MARKERT A, ZILLIG W. STUDIES ON THE LYSIS OF ESCHERICHIA COLI C BY BACTERIOPHAGE PHI-X174. Virology. 1965 Jan;25:88–97. [PubMed]
  • Mazza A, Felluga B. Electron microscope observations on lambda bacteriophage ultrastructure. J Ultrastruct Res. 1973 Nov;45(3):259–278. [PubMed]
  • Panayotatos N, Truong K. Specific deletion of DNA sequences between preselected bases. Nucleic Acids Res. 1981 Nov 11;9(21):5679–5688. [PMC free article] [PubMed]
  • Queen C, Rosenberg M. A promoter of pBR322 activated by cAMP receptor protein. Nucleic Acids Res. 1981 Jul 24;9(14):3365–3377. [PMC free article] [PubMed]
  • Stüber D, Bujard H. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A. 1981 Jan;78(1):167–171. [PMC free article] [PubMed]
  • Thuring RW, Sanders JP, Borst P. A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975 May 26;66(1):213–220. [PubMed]
  • Tsugita A, Inouye M. Complete primary structure of phage lysozyme from Escherichia coli T4. J Mol Biol. 1968 Oct 14;37(1):201–212. [PubMed]
  • von Heijne G. Membrane proteins: the amino acid composition of membrane-penetrating segments. Eur J Biochem. 1981 Nov;120(2):275–278. [PubMed]

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