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Toxicol Sci. Nov 2008; 106(1): 5–28.
Published online Jun 19, 2008. doi:  10.1093/toxsci/kfn121
PMCID: PMC2563142

Caenorhabditis elegans: An Emerging Model in Biomedical and Environmental Toxicology

Abstract

The nematode Caenorhabditis elegans has emerged as an important animal model in various fields including neurobiology, developmental biology, and genetics. Characteristics of this animal model that have contributed to its success include its genetic manipulability, invariant and fully described developmental program, well-characterized genome, ease of maintenance, short and prolific life cycle, and small body size. These same features have led to an increasing use of C. elegans in toxicology, both for mechanistic studies and high-throughput screening approaches. We describe some of the research that has been carried out in the areas of neurotoxicology, genetic toxicology, and environmental toxicology, as well as high-throughput experiments with C. elegans including genome-wide screening for molecular targets of toxicity and rapid toxicity assessment for new chemicals. We argue for an increased role for C. elegans in complementing other model systems in toxicological research.

Keywords: Caenorhabditis elegans, neurotoxicity, genotoxicity, environmental toxicology, high-throughput methods

Caenorhabditis elegans is a saprophytic nematode species that has often been described as inhabiting soil and leaf-litter environments in many parts of the world (Hope, 1999); recent reports indicate that it is often carried by terrestrial gastropods and other small organisms in the soil habitat (Caswell-Chen et al., 2005; Kiontke and Sudhaus, 2006). Although scientific reports on the species have appeared in the literature for more than 100 years (e.g., Maupus, 1900), the publication of Brenner's seminal genetics paper (Brenner, 1974) signaled its emergence as an important experimental model. Work with C. elegans has since led in a short time span to seminal discoveries in neuroscience, development, signal transduction, cell death, aging, and RNA interference (Antoshechkin and Sternberg, 2007). The success of C. elegans as a model has attracted increased attention as well in the fields of in biomedical and environmental toxicology.

Clearly, C. elegans will be a valuable toxicity model only if its results were predictive of outcomes in higher eukaryotes. There is increasing evidence that this is the case both at the level of genetic and physiological similarity and at the level of actual toxicity data. Many of the basic physiological processes and stress responses that are observed in higher organisms (e.g., humans) are conserved in C. elegans. Depending on the bioinformatics approach used, C. elegans homologues have been identified for 60–80% of human genes (Kaletta and Hengartner, 2006), and 12 out of 17 known signal transduction pathways are conserved in C. elegans and human (NRC, 2000; Table 1). We discuss specific examples in the areas of neurotoxicology and genetic toxicology in this review.

TABLE 1
Signal Transduction Pathways Conserved in Nematodes and Vertebratesab

Caenorhabditis elegans has a number of features that make it not just relevant but quite powerful as a model for biological research. First of all, C. elegans is easy and inexpensive to maintain in laboratory conditions with a diet of Escherichia coli. The short, hermaphroditic life cycle (~3 days) and large number (300+) of offspring of C. elegans allows large-scale production of animals within a short period of time (Hope, 1999). Since C. elegans has a small body size, in vivo assays can be conducted in a 96-well microplate. The transparent body also allows clear observation of all cells in mature and developing animals. Furthermore, the intensively studied genome, complete cell lineage map, knockout (KO) mutant libraries, and established genetic methodologies including mutagenesis, transgenesis, and RNA interference (RNAi) provide a variety of options to manipulate and study C. elegans at the molecular level (Tables 2 and and3;3; for a more detailed presentation of genetic and genomic resources, see Antoshechkin and Sternberg, 2007). We address the particular power of these genetic and molecular tools in C. elegans at more length below.

TABLE 2
Examples of Mutational Analysis of Caenorhabditis elegans in Toxicology Research
TABLE 3
Examples of Transgenic Caenorhabditis elegans Used in Toxicology Research

Since reverse genetic and transgenic experiments are much easier and less expensive to conduct in C. elegans as compared to many other model systems, it is a useful model for molecular analyses of the response of conserved pathways to in vivo chemical exposure. As an in vivo model, C. elegans provides several characteristics that complement in vitro or cellular models. The use of whole-organism assays, first of all, allows the study of a functional multicellular unit, such as a serotonergic synapse, instead of a single cell (Kaletta and Hengartner, 2006). Caenorhabditis elegans also enables the detection of organism-level end points (e.g., feeding, reproduction, life span, and locomotion) and the interaction of a chemical with multiple targets in an organism. Thus, C. elegans complements both in vitro and in vivo mammalian models in toxicology.

Of note, these characteristics facilitate high-throughput experiments that can examine both fundamental toxicity, which are critical since so many chemicals have yet to be thoroughly tested, and the gene-gene and gene-environment interactions whose importance is just beginning to be appreciated in toxicology.

Here we review three major applications of C. elegans in biomedical and environmental toxicology: (1) mechanistic toxicology, with a focus on neurotoxicity and genotoxicity; (2) high-throughput screening capabilities; and (3) environmental toxicology and environmental assessment. We emphasize studies of neurotoxicity because they are the area of toxicology in which C. elegans has been most exploited to date. We discuss research methods, recent advances, and important considerations including limitations of the C. elegans model.

Caenorhabditis elegans AND NEUROTOXICITY

Caenorhabditis elegans Is Well Suited for Neurophysiology Analysis of Neurotoxicity

With 302 neurons representing 118 characterized neuronal subtypes (Hobert, 2005), C. elegans provides an in vivo model for studying mechanisms of neuronal injury with resolution of single neurons. It intially underwent extensive development as a model organism in order to study the nervous system (Brenner, 1974), and its neuronal lineage and the complete wiring diagram of its nervous system are stereotyped and fully described (Sulston, 1983; Sulston et al., 1983; White et al., 1986). Each neuron has been assigned a code name corresponding to its location. For example, ADEL describes the dopaminergic (DAergic) head neuron “anterior deirid left.” This relatively “simple” nervous system is comprised of 6393 chemical synapses, 890 electrical junctions, and 1410 neuromuscular junctions (Chen et al., 2006). Additionally, the main neurotransmitter systems (cholinergic, γ-aminobutyric acid (GABA)ergic, glutamatergic, DAergic, and serotoninergic) and their genetic networks (from neurotransmitter metabolism to vesicle cycling and synaptic transmission) are phylogenetically conserved from nematodes to vertebrates, which allows for findings from C. elegans to be extrapolated and further confirmed in vertebrate systems.

Several genes involved in neurotransmission were originally identified in C. elegans. This is exemplified by the GABA vesicular transporter unc-47 and the regulatory transcription factor unc-30 (for review on the GABAergic system [Jorgensen, 2005]), the vesicular acetylcholine (ACh) transporter unc-17 (for review on the cholinergic system [Rand, 2007]), the glutamate-gated chloride channel subunits α1 and β (glc-1 and glc-2, respectively, for review on the glutamatergic system [Brockie and Maricq, 2006]), and the synaptic proteins unc-18, unc-13, unc-26 (for review on synaptic function [Richmond, 2005]). Experiments challenging the C. elegans nervous system by laser ablation of individual neurons/axons, exposure to drugs, and other external stimuli have facilitated the design of robust behavioral tests to assess the function of defined neuronal populations (Avery and Horvitz, 1990; Bargmann, 2006; Barr and Garcia, 2006; Brockie and Maricq, 2006; Chase and Koelle, 2007; Goodman, 2006; Morgan et al., 2007; Rand, 2007). For example, inhibitory GABAergic and excitatory cholinergic motor functions are assessed by quantifying the sinusoidal movement (amplitude and frequency of body bends) and foraging behavior of the worm. Motor and mechanosensory functions of glutamatergic neurons are evaluated by measuring the pharyngeal pumping rate and the response to touch. Mechanosensory functions of DAergic and serotoninergic neurons are appraised by observing the ability of worms to slow down when they encounter food. Furthermore, the creation of transgenic strains expressing fluorescent proteins in defined neurons allows in vivo imaging of any desired neuron. While experimentally challenging in the cells of microscopic animals, electrophysiology studies can be conducted with relative ease and success in live worms and cultured C. elegans neurons, establishing that they are electrophysiologically comparable to vertebrate neurons in their response to various drugs (Bianchi and Driscoll, 2006; Brockie and Maricq, 2006; Cook et al., 2006; Schafer, 2006). Given the relative ease with which gene KO and transgenic animals can be generated, the ability to culture embryonic or primary C. elegans cells offers unique perspectives for neurotoxicology applications and study designs.

Caenorhabditis elegans Is a Potent Model to Decipher Genetic Aspects of Neurotoxicity

The conservation of neurophysiologic components from nematodes to humans largely relies on shared genetic networks and developmental programs. Hence, the availability of mutants for many of the C. elegans genes facilitated significant progress in unraveling of evolutionarily conserved cellular and genetic pathways responsible for neuron fate specificity (Hobert, 2005), differentiation (Chisholm and Jin, 2005), migration (Silhankova and Korswagen, 2007), axon guidance (Quinn and Wadsworth, 2006; Wadsworth, 2002), and synaptogenesis (Jin, 2002, 2005). Recently, laser axotomy in C. elegans has been successfully applied to identify axon regeneration mechanisms (Gabel et al., 2008; Wu et al., 2007), which are of utmost importance in developing treatments to reverse neurodegenerative processes and spinal cord injuries. Essential cell functions relevant to neurotoxicity studies are also conserved. This is best exemplified by the mechanistic elucidation of the apoptotic pathway in C. elegans, for which the 2002 Nobel Prize in Physiology or Medicine was awarded (Hengartner and Horvitz, 1994; Horvitz, 2003; Sulston, 2003). The pathway is of direct interest to neurotoxicologists since apoptosis is implicated in many neurodegenerative diseases and toxicant-induced cell demise (Bharathi et al., 2006; Hirata, 2002; Koh, 2001; Mattson, 2000; Ong and Farooqui, 2005; Savory et al., 2003). Pathways relevant to oxidative stress–related neuronal injuries, such as the p38 mitogen-activated protein kinase and AKT signaling cascades, the ubiquitin-proteasome pathway, and the oxidative stress response are also conserved in the worm (Ayyadevara et al., 2005, 2008; Daitoku and Fukamizu, 2007; Gami et al., 2006; Grad and Lemire, 2004; Inoue et al., 2005; Kipreos, 2005; Leiers et al., 2003; Tullet et al., 2008; Wang et al., 2007a).

The nematode model is also amenable to interesting genetic alterations. Hence, it is very easy to generate transgenic worms expressing any kind of mutant recombinant protein, providing means for the study of neurodegenerative diseases (see additional discussion below). Gene KO and altered function mutations are in many cases available from the Gene Knockout Consortium or the National BioResource Project of Japan (currently ~1/3 of the ~20,000 total genes in C. elegans; Antoshechkin and Sternberg, 2007) or alternatively are conveniently generated using chemicals, radiations, or transposons (discussed below under Caenorhabditis elegans and Genotoxicity). Hence, classical approaches to elucidate intracellular pathways in C. elegans include forward and modifier screens following random mutagenesis (Inoue and Thomas, 2000; Malone and Thomas, 1994; Morck et al., 2003; Nass et al., 2005; O'Connell et al., 1998). Finally C. elegans is amenable to gender manipulation (possible generation of males, feminized males, masculinized hermaphrodites, or feminized hermaphrodites) permitting studies on sex specificity mechanisms of neurotoxicants or disorders and “rejuvenation” by forcing development through the quiescent dauer larval stage (Houthoofd et al., 2002).

Neurotoxicological Studies in C. elegans

Years before the latest technologic developments (RNAi and high-throughput techniques), C. elegans was used to study toxicity mechanisms of environmental factors affecting the nervous system. The following section provides a synopsis of the available literature on neurotoxicity-related issues addressed in C. elegans. It is not meant to be exhaustive but rather to illustrate typical studies that are amenable in the C. elegans platform. We highlight studies with exposure outcomes to various metals and pesticides, as well as general considerations on studies of neurodegenerative diseases. We emphasize the utility of C. elegans in addressing hypothesis-driven mechanisms of neurotoxicity and extrapolations to vertebrate systems.

Toxicity Mechanisms of Neurotoxic Metals in C. elegans

Caenorhabditis elegans has been used as a model system to elucidate the toxicity and toxicological mechanisms of various heavy metals, such as Aluminum (Al), Arsenic (As), Barium (Ba), Cadmium (Cd), Copper (Cu), Lead (Pb), Mercury (Hg), Uranium (U), and Zinc (Zn). In general, these studies focused on various toxic end points, such as lethality, reproduction, life span, and protein expression. Some focus has also been directed to the effects of these metals on the nervous system by assessing behavior, reporter expression and neuronal morphology. We provide here a few examples of these approaches.

Investigators have performed numerous studies to assess behavior-induced alterations following exposure of the worm to heavy metals. Depending on the end point assessed, neurotoxic effects on specific neuronal circuitries can be inferred.

For instance, a defect in locomotion reflects an impairment of the neuronal network formed by the interneurons AVA, AVB, AVD, and PVC providing input to the A- and B-type motor neurons (responsible for forward and backward movement) and the inhibitory D-type motor neurons involved in the coordination of movement (Riddle et al., 1997). By recording short videos and subsequently analyzing them using computer tracking software, it has been possible to quantify the overall movement of C. elegans (distance traveled, directional change, etc.), body bends and head thrashes, upon metal treatments, allowing to further correlate the data with damages to neuron circuitries. These computer tracking studies showed that worms displayed a dose-dependent decrease in locomotory movement upon exposure to Pb (Anderson et al., 2001, 2004; Johnson and Nelson, 1991) and Al (Anderson et al., 2004), while an increase in locomotion was observed upon exposure to low concentrations of Hg as compared with Cu (Williams and Dusenbery, 1988). Another study showed that exposure to Ba impaired both body bend and head thrashing rates in a dose-dependent manner (Wang et al., 2008), corroborating mammalian data on the effect of Ba on the nervous system attributed to its ability to block potassium channels (Johnson and Nelson, 1991).

Feeding behavior has also been shown to be affected upon heavy metal exposure. Feeding requires a different neuronal circuitry including M3 (involved in pharyngeal relaxation), MC (control of pumping rate), M4 (control of isthmus peristalsis), NSM (stimulate feeding), RIP, and I neurons (Riddle et al., 1997). A decrease in feeding was observed when worms were exposed to Cd or Hg (Boyd et al., 2003; Jones and Candido, 1999).

Behavioral research studying the effect of heavy metals on C. elegans has also taken the route of assessing the ability of the worm to sense the toxin and alter its behavior accordingly, involving other neural circuitry, such as the amphid and phasmid neurons responsible for chemosensation (Riddle et al., 1997). By generating concentration gradient–containing plates, Sambongi et al. (1999) discovered that C. elegans was able to avoid Cd and Cu but not Ni and that the amphid ADL, ASE, and ASH neurons were responsible for this avoidance as their combined ablation eliminated the avoidance phenotype. Furthering the investigation into the role of ASH neurons, researchers found that a calcium (Ca2+) influx could be elicited upon exposing the C. elegans to Cu, which may provide insight into the mechanism of the ability of the worm to display avoidance behaviors (Hilliard et al., 2005).

Caenorhabditis elegans exhibits both short-term and long-term learning-related behaviors in response to specific sensory inputs (Rankin et al., 1990), which involve defined neuronal networks. As an example, thermosensation-associated learning and memory rely on the AFD sensory neuron sending inputs to the AIY and AIZ interneurons, whose signals are integrated by the RIA and RIB interneurons to command the RIM motor neuron (Mori et al., 2007). When assessing the function of this circuitry, worms grown and fed at a definite temperature are moved to a food-deprived test plate exposed to a temperature gradient. The ability of the worms to find and remain in the area of the test plate corresponding to the feeding temperature reflects the functioning of the thermosensation learning and memory network aforementioned (Mori et al., 2007). Interestingly, worms exposed to Al and Pb exhibit poor scores at this test, indicative of a significant reduction of the worms’ learning ability (Ye et al., in press). This recapitulates the learning deficits observed in young patients overexposed to the same metals (Garza et al., 2006; Goncalves and Silva, 2007).

While behavioral testing was informative of the neuronal circuitries affected by heavy metals, additional experiments uncovered the molecular mechanisms of their neurotoxic effects. For example, in the previously described study, after determining that Al and Pb induced memory deficits, the investigators showed that the antioxidant vitamin E effectively reversed these deficits, indicating a role of oxidative stress in Al and Pb neurotoxicity (Ye et al., in press). The involvement of oxidative stress in metal-induced toxicity was further confirmed when worms mutated in glutamylcysteine synthetase (gcs-1), the rate-limiting enzyme in glutathione synthesis exhibited hypersensitivity to As exposure when compared to wild-type animals (Liao and Yu, 2005).

Studies conducted in mammalian models found that Hg is able to block Ca2+ channels. In neurons, this blockage can induce spontaneous release of neurotransmitters (Atchison, 2003). In C. elegans, the Ca2+ channel blocker verapamil was found to protect against Hg exposure, suggesting that Ca2+ signaling plays a role in the toxicity of Hg in this model organism as in mammals (Koselke et al., 2007).

Observation of neuron morphology following heavy metal exposure was also performed using C. elegans strains expressing the green fluorescent protein (GFP) in discrete neuronal populations. Tests using depleted U evoked no alterations in the DAergic nervous system of C. elegans, an observation corroborated with data from mammalian primary neuronal cultures (Jiang et al., 2007). Meanwhile, kel-8 and numr-1, which are involved in resistance to Cd toxicity, were upregulated upon Cd exposure. In particular, GFP levels of KEL-8::GFP and NUMR-1::GFP were increased in the pharynx and the intestine in addition to the constitutive expression observed in AWA neurons (Cui et al., 2007a; Freedman et al., 2006; Jackson et al., 2006; Tvermoes and Freedman, 2008). Furthermore, numr-1 was shown to be induced in response to heavy metals, such as Cd, Cu, Cobalt (Co), Chromium (Cr), Ni, As, Zn, and Hg. NUMR-1::GFP was localized to nuclei within the intestine and the pharynx and colocalized with the stress-responsive heat-shock transcription factor HSF-1::mCherry (Tvermoes and Freedman, 2008). This indicates that these particular genes were altered in response to heavy metals and this may aid in the understanding of the toxicity of or the protection against these agents.

Toxicity Mechanisms of Neurotoxic Pesticides in C. elegans

Currently, there are over a hundred types of pesticides available and substantial efforts have been put forth to examine the neurotoxicity of these agents. Similarity in neural circuitry and the conservation in genetic makeup between C. elegans and humans have led to a number of recent studies on pesticide neurotoxicity in this species (summarized in Table 4). In this section, we discuss the effects of three groups of pesticides on neurological pathways in C. elegans and their relevance to understanding mechanisms of human neurotoxicity.

TABLE 4
Pesticides that Have Been Tested Using Caenorhabditis elegans as a Model Organism

Paraquat, also known as methyl viologen (mev), is mainly used as an herbicide. Increased concerns for the potential human risks associated with paraquat exposure stems from studies indicating that subjects experiencing exposure to this and other herbicides/insecticides have a higher prevalence of Parkinson disease (PD) (Liou et al., 1997; Semchuk et al., 1992) (Gorell et al., 1998) and increased mortality from PD (Ritz and Yu, 2000). The use of C. elegans to study the etiology of PD will be discussed in the later section. This is due to the specificity with which these pesticides target the nigrostriatal DAergic system via an elevation of dopamine and amine turnover (Thiruchelvam et al., 2000a, 2000b). All forms of paraquat are easily reduced to a radical ion, which generates superoxide radical that reacts with unsaturated membrane lipids (Uversky, 2004), a likely mechanism of neurotoxicity. Caenorhabditis elegans, has a well-defined, yet simple DAergic network, consisting of eight neurons in the hermaphrodite and an additional six neurons located in the tail of the male (Chase and Koelle, 2007) and four DA receptors. Dopamine is known to be required in the modulation of locomotion and in learning in C. elegans (Hills et al., 2004; Sanyal et al., 2004; Sawin et al., 2000). To date, several paraquat/mev–altered strains have been generated to study potential pathways in which paraquat exerts its toxic effects. mev-1 (mutated for the succinate dehydrogenase) (Hartman et al., 1995; Ishii et al., 1990; Kondo et al., 2005) and mev-3 (Yamamoto et al., 1996) were generated first, and both strains displayed increased sensitivity to paraquat- and oxygen-mediated injury as a result of increased production of superoxide radicals (Guo and Lemire, 2003; Ishii et al., 1990) and hypersensitivity to oxidative stress. mev-4 (Fujii et al., 2004), mev-5, mev-6, and mev-7 (Fujii et al., 2005) displayed resistance to paraquat. However, since the proteins that are encoded by these genes are currently unknown, future mapping of these genes will likely reveal pathways involved in paraquat toxicity.

Paraquat exerts oxidative damage in vertebrates, which has also been corroborated in C. elegans. Mutants that lack antioxidant enzymes such as cytosolic or mitochondrial superoxide dismutases (sod-1 and sod-2) show increased sensitivity to paraquat (Yang et al., 2007), whereas mutants with increased superoxide dismutase levels, such as age-1 (encoding the catalytic subunit of phosphoinositide 3-kinase) (Vanfleteren, 1993; Yanase et al., 2002) and worms overexpressing the omega-class glutathione transferase gsto-1 (Burmeister et al., 2008) display increased resistance to paraquat toxicity. Moreover, C. elegans mutants hypersensitive to oxygen toxicity, such as rad-8 (Honda et al., 1993; Ishii et al., 1990) or those with a prolonged life span, such as daf-2 (encoding insulin/insulin growth factor receptor) (Bardin et al., 1994; Kim and Sun, 2007) show increased tolerance to paraquat. Taken together, these results provide novel information on mechanisms by which paraquat mediates its toxicity (by enhancing sensitivity to oxygen toxicity with an elevation in production of reactive oxygen species and shortening life span) and provide directions for future investigations on mechanisms that lead to DAergic neurodegeneration.

A second ubiquitous pesticide is rotenone; it is a naturally occurring and biodegradable pesticide effective in killing pests and fish (Uversky, 2004). Researchers first reported in 2000 that Iv exposure to rotenone may lead in humans to the development of PD-like symptoms accompanied by the selective destruction of nigral DAergic neurons (Betarbet et al., 2000). Since rotenone acts by inhibiting mitochondrial NADH dehydrogenase within complex I (Gao et al., 2003), the development of a mutant C. elegans strain that exhibits mitochondrial inhibition provided an experimental platform where the role of this enzyme could be directly evaluated. A mutation in a 49-kDa subunit of mitochondrial complex I in C. elegans mutant gas-1 displays hypersensitivity to rotenone and oxygen (Ishiguro et al., 2001), highlighting the importance of a functional complex I in rotenone resistance. Moreover, C. elegans with alterations in PD causative genes are highly sensitive to rotenone toxicity, suggesting the ability of these proteins to protect against rotenone-induced oxidative damage in DAergic neurons (Ved et al., 2005; Wolozin et al., 2008) (see neurodegenerative disease section below).

The organophosphates (OPs) are a group of insecticides that target the cholinergic system. ACh is the primary neurotransmitter involved in motor function in most organisms, including the nematode (Rand and Nonet, 1997). Due to the involvement of the neuromuscular system, a computer tracking system was used to study the neurobehavioral changes in C. elegans associated with two OP pesticides (malathion and vapona). Caenorhabditis elegans showed a remarkable decline in locomotion at a concentration below survival reduction (Williams and Dusenbery, 1990b). Comparison studies using similar behavioral analyses were later developed to assess movement alteration as an indicator of the neurotoxity of 15 OP pesticides (Cole et al., 2004) and carbamate pesticides, which unlike OP pesticides are reversible AChE inhibitors (Melstrom and Williams, 2007). The LD50 values in C. elegans closely correlated with LD50 in both rats and mice. Pesticides (vapon, parathion, methyl parathion, methidathion, and funsulfothion) that showed cholinesterase inhibition were associated with pronounced behavioral toxicity (i.e., decrease in movement). A recent study has compared end points using OPs and found AChE inhibition to be the most sensitive indicator of toxicity but also the most difficult to measure (Rajini et al., in press). Reduction in movement for 10 OPs was found to correlate to rat and mouse acute lethality data. Finally, simulation studies examining the rate of absorption and biodegradation of OP (parathion) also (Saffih-Hdadi et al., 2005) establish the relevance and reliability of C. elegans as an experimental model and predictor for soil toxicity.

Caenorhabditis elegans in the Study of Neurodegeneration

As previously stated, the C. elegans nervous system functionally recapitulates many of the characteristics of the vertebrate brain. In particular, it can undergo degeneration through conserved mechanisms and is thus a powerful model for uncovering the genetic basis of neurodegenerative disorders. In this section, we will focus on PD, Alzheimer disease (AD), Huntington disease (HD), and Duchenne muscular dystrophy (DMD).

PD is a progressive, neurodegenerative disorder afflicting ~2% of the U.S. population (Bushnell and Martin, 1999). Characteristic features include a gradual loss of motor function due to the degeneration of DAergic neurons within the substantia nigra pars compacta and loss of DAergic terminals in the striatum (Wilson et al., 1996). At the cellular level, deposition of cytoplasmic Lewy bodies composed of aggregated protein, such as α-synuclein, is observed. PD cases are referred as familial (FPD) or idiopathic (IPD) depending on whether the disease is hereditary (FPD) or from unknown origin, possibly due to environmental exposure to neurotoxicants (IPD) (Dauer and Przedborski, 2003; Samii et al., 2004). Among 11 genomic regions (PARK1 to 11) associated with FPD, 7 were narrowed down to single genes: PARK1 (α-SYNUCLEIN), PARK2 (PARKIN), PARK4 (α-SYNUCLEIN), PARK5 (UCHL1), PARK6 (PINK1), PARK7 (DJ1), PARK8 (DARDARIN/LRRK2), and PARK9 (ATP13A2) (Wood-Kaczmar et al., 2006). All but α-SYNUCLEIN are strictly conserved in the nematode with most residue positions mutated in PD patients encoding identical amino acids in C. elegans orthologues (Benedetto et al., 2008). Worms overexpressing wild type, mutant A30P, or A53T human α-SYNUCLEIN in DAergic neurons show differential levels of injury, including reduced DA content, DAergic neuron degeneration, motor deficits reversible by DA administration, intracellular α-SYNUCLEIN aggregates similar to Lewy bodies, and increased vulnerability to mitochondrial complex-I inhibitors, which is reversed by treatment with antioxidants (Kuwahara et al., 2006; Lakso et al., 2003; Ved et al., 2005). Furthermore, deletion (Springer et al., 2005) and knockdown of the C. elegans PARKIN and DJ1 genes produce similar patterns of pharmacological vulnerability as those described above for α-SYNUCLEIN overexpression (Ved et al., 2005). Other PD genes in C. elegans have been investigated. For example, ubh-1 and ubh-3 (Chiaki Fujitake et al., 2004) share similar functions with the human PARK5/UCHL1 orthologue. Studies on other genes have been instrumental in unraveling previously unknown functions. For example, examination of the PARK8/DARDARIN orthologue lrk-1 showed that the protein allows the proper targeting of synaptic vesicle proteins to the axon (Sakaguchi-Nakashima et al., 2007) and protects against rotenone-induced mitochondrial injury (Wolozin et al., 2008). Recently, RNAi, genomic, and proteomic approaches using human α-SYNUCLEIN transgenic worms identified genetic networks linking PD to G-protein signaling, endomembrane trafficking, actin cytoskeleton, and oxidative stress (Cooper et al., 2006; Gitler et al., 2008; Hamamichi et al., 2008; Ichibangase et al., 2008; van Ham et al., 2008; Vartiainen et al., 2006), illustrating the power of this transgenic model for PD study.

Nonhereditary PD cases have also been associated with exposure to 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine, a designer drug that is converted intracerebrally (by astrocytes) to 1-methyl-4-phenylpyridinium (MPP+) by the monoamine oxygenase B. MPP+ damages the DAergic nervous system, leading to a typical Parkinsonian syndrome (Kopin and Markey, 1988; Langston et al., 1984). Similarly, MPP+-exposed C. elegans show specific degeneration of DAergic neurons and associated behavioral defects (Braungart et al., 2004), which is due to ATP depletion (Wang et al., 2007b). Exposures to rotenone (see above) or 6-hydroxydopamine also lead to PD syndromes that share similar features both in humans and worms (Cao et al., 2005; Ishiguro et al., 2001; Marvanova and Nichols, 2007; Nass et al., 2002, 2005; Ved et al., 2005). Though the nematode does not truly exhibit PD-like symptoms, results with transgenic and drug-exposed worms emphasize the relevance of C. elegans as a model organism that (1) permits rapid insights in the genetic pathways involved in PD and (2) enables high-throughput screening methods for the development of new anti-PD drugs (Schmidt et al., 2007).

Tauopathies and polyglutamine extension disorders have also been investigated in the worm using mutants and transgenic strains (Brandt et al., 2007; Dickey et al., 2006, Link, 2001; Kraemer et al., 2003, 2006, and Kraemer and Schellenberg, 2007). The first AD-associated proteins identified were the beta-amyloid peptide precursor (betaAPP) and the presenilins PS1 and PS2. Study of the C. elegans presenilin orthologues sel-12 (Baumeister et al., 1997; Levitan and Greenwald, 1995) and hop-1 (Li and Greenwald, 1997; Smialowska and Baumeister, 2006) linked AD to the apoptotic pathway (Kitagawa et al., 2003) and Notch signaling, which was later confirmed in vertebrates (Berezovska et al., 1998, 1999; Ray et al., 1999). Characterization of the C. elegans betaAPP orthologue revealed a key role for microRNA in AD gene regulation (Niwa et al., 2008). However, most of the knowledge about AD acquired in C. elegans came from two transgenic models: worms expressing the human betaAPP (Boyd-Kimball et al., 2006; Drake et al., 2003; Gutierrez-Zepeda and Luo, 2004; Wu and Luo, 2005; Wu et al., 2006) or TAU (Brandt et al., in press; Kraemer et al., 2003). Studies on betaAPP transgenic worms revealed toxicity mechanisms of AD by identifying two new genes, aph-1 and pen-2, likely involved in the progression of the disease (Boyd-Kimball et al., 2006; Francis et al., 2002). They also allowed the characterization of oxidation processes preceding fibrillar deposition (Drake et al., 2003) and the identification of genes activated upon induction of betaAPP expression (Link et al., 2003). Furthermore, protective mechanisms were identified (Florez-McClure et al., 2007; Fonte et al., 2008) and potential therapeutic drugs for AD (ginkgolides, Ginkgo biloba extract EGb 761, soy isoflavone glycitein) were originally and successfully assayed in worms (Gutierrez-Zepeda et al., 2005; Luo, 2006; Wu et al., 2006). Caenorhabditis elegans overexpressing the human TAU or a pseudohyperphosphorylated mutant TAU were found to exhibit age-dependent motor neuron dysfunctions, neurodegeneration, and locomotor defects due to impaired neurotransmission (Brandt et al., 2007; Kraemer et al., 2003).

Likewise, while a few Huntingtin (Htt)-interacting genes were identified in C. elegans (Chopra et al., 2000; Holbert et al., 2003), most data came from transgenic worms expressing polyQ variants of Htt. Several groups targeted different neuronal subsets to study polyQHtt neurotoxicity in the worm. They described behavioral defects prior to neurodegeneration and protein aggregation and axonal defects and uncovered a role for apoptosis in HD neurodegeneration (Bates et al., 2006; Faber et al., 1999; Holbert et al., 2003; Parker et al., 2001). Protective mechanisms of the polyQ enhancer-1 and ubiquilin were demonstrated (Faber et al. 2002; Wang et al., 2006), and pharmacological screening using polyQHtt transgenic C. elegans is ongoing (Faber et al. 2002; Wang et al., 2006).

A final illustration of the successful use of C. elegans in elucidating the genetic basis of neurodegenerative disorder is exemplified by the characterization of the genetic network implicated in DMD. DMD is mainly characterized by a progressive loss of muscular mass and function occurring in males due to mutations in the DYSTROPHIN gene located on the X chromosome, which commonly leads to paralysis and death by the age of 30. DYSTROPHIN is both muscular and neuronal, being required for brain architecture and neurotransmission, such that DMD patients exhibit neurodegeneration associated with motor deficits and reduced cognitive performances (average IQ is 85 in DMD boys) (Anderson et al., 2002; Blake and Kroger, 2000; Poysky, 2007). DYSTROPHIN is conserved in C. elegans, but its loss-of-function in the worm results in hypercontractility due to impaired cholinergic activity and does not affect muscle cells (Bessou et al., 1998; Gieseler et al., 1999b). Nevertheless, the observation that double mutants for Dystrophin/dys-1 and MyoD/hlh-1 display severe and progressive muscle degeneration in the worm (as observed in mice), set up the basis for a C. elegans model to study dystrophin-dependent myopathies (Gieseler et al., 2000). Using this model, several partners of DYSTROPHIN were characterized, establishing their role in cholinergic neurotransmission and muscle degeneration (Gieseler et al., 1999a, 1999b, 2001; Grisoni et al., 2002a, 2002b, 2003). Additionally, it was shown that the overexpression of DYSTROBREVIN/dyb-1 delays neurological and muscular defects (Gieseler et al., 2002), and mutations in CHIP/chn-1, chemical inhibition of the proteasome, and prednisone or serotonin treatments suppress muscle degeneration in C. elegans (Carre-Pierrat et al., 2006; Gaud et al., 2004; Nyamsuren et al., 2007).

Thus, though at first glance C. elegans appears quite different from vertebrates, its nervous circuitry and the cellular processes guiding neuronal development, neuronal death or survival, neurotransmission, and signal integration rely on the same neuronal and molecular networks as vertebrates. Combined with the advantages of a small and fast-growing organism, these properties make C. elegans a perfect system for rapid genetic analysis of neurotoxicity mechanisms.

Caenorhabditis elegans AND GENOTOXICITY

As is the case for neurotoxicity, C. elegans provides a cost-effective, in vivo, genetically manipulable and physiological model for the study of the toxicological consequences of DNA damage. As described below, the machinery that responds to DNA damage in C. elegans is very similar genetically to the corresponding machinery in higher eukaryotes. Many processes related to DNA damage have been extensively studied in C. elegans, providing an important biological context and clear relevance to mechanistic studies. Finally, powerful tools for the study of DNA damage, DNA repair, and mutations have been developed in this organism.

DNA Damage Response Proteins Are Conserved between C. elegans and Higher Eukaryotes

Genes and pathways involved in DNA repair in mammals are generally well conserved in C. elegans (Boulton et al., 2002; Hartman and Nelson, 1998; O'Neil and Rose, 2005). Proteins involved in nucleotide excision repair, mismatch repair, homologous recombination, and nonhomologous end joining, for instance, are almost entirely conserved between C. elegans, mouse, and human based on nucleotide sequence homology (http://www.niehs.nih.gov/research/atniehs/labs/lmg/dnarmd/docs/Cross-species-comparison-of-DNA-repair-genes.xls). This is also true for proteins involved in many DNA repair–related processes, such as translesion DNA polymerases, helicases, and nucleases. Base excision repair proteins, interestingly, show somewhat less conservation. While this conservation is based in some cases only on sequence homology, many of these proteins have now been biochemically or genetically characterized. Critically, proteins involved in other DNA damage responses including apoptosis and cell cycle arrest are also conserved in C. elegans and mammals (Stergiou and Hengartner, 2004).

DNA Repair in C. elegans

Early studies on DNA repair in C. elegans were carried out by Hartman and colleagues, who identified a series of radiation-sensitive mutants (Hartman, 1985; Hartman and Herman, 1982) and used an antibody-based assay to measure induction and repair of ultraviolet (UV) radiation–induced damage (Hartman et al., 1989). These and more recent studies (Hyun et al., 2008; Meyer et al., 2007) have shown that nucleotide excision repair is similar in C. elegans and humans both in terms of conservation of genes and kinetics of repair. Nucleotide excision repair is a critical pathway in the context of exposure to environmental toxins since it recognizes and repairs a wide variety of bulky, helix-distorting DNA lesions, including polycyclic aromatic hydrocarbon metabolites, mycotoxins such as aflatoxin B1, UV photoproducts, cisplatin adducts, and others (Friedberg et al., 2006; Truglio et al., 2006).

While nucleotide excision repair has been the best-studied DNA repair pathway in C. elegans, significant progress has been made in the study of genes involved in other DNA repair pathways as well. The role of specific C. elegans gene products in DNA repair has been studied both via high-throughput and low-throughput methods. High-throughput methods including RNAi knockdown and yeast two-hybrid analysis of protein-protein interaction have been used to identify a large number of genes coding for proteins involved in responding to DNA damage (Boulton et al., 2002; van Haaften et al., 2004a, 2004b). Lower throughput studies involving biochemical analyses of DNA repair activities (Dequen et al., 2005a; Gagnon et al., 2002; Hevelone and Hartman, 1988; Kanugula and Pegg, 2001; Munakata and Morohoshi, 1986; Shatilla et al., 2005a, 2005b; Shatilla and Ramotar, 2002) as well in vivo sensitivity to DNA damaging agents (Astin et al., 2008; Boulton et al., 2004; Dequen et al., 2005b; Lee et al., 2002, 2004; Park et al. 2002, 2004; St-Laurent et al., 2007) or other DNA damage–related phenotypes (Aoki et al., 2000; Kelly et al., 2000; Sadaie and Sadaie, 1989; Takanami et al., 1998) have supported the sequence similarity–based identification of C. elegans homologues of DNA repair genes in higher vertebrates, as well as in some cases permitting identification of previously unknown genes involved in these pathways.

Apoptosis and Cell Cycle Checkpoints in C. elegans

DNA damage that is not repaired can trigger cell cycle arrest and apoptosis, and these pathways are very well studied in C. elegans. The great progress made in understanding them mechanistically demonstrates the power of this model organism. As mentioned, the cellular mechanisms regulating apoptosis were discovered in C. elegans, and apoptosis and cell cycle responses to DNA damage continue to be heavily studied in C. elegans (Ahmed et al., 2001; Ahmed and Hodgkin, 2000; Conradt and Xue, 2005; Gartner et al., 2000; Jagasia et al., 2005; Kinchen and Hengartner, 2005; Lettre and Hengartner, 2006; Olsen et al., 2006; Schumacher et al., 2005; Stergiou et al., 2007). The short life span of C. elegans has especially lent itself to groundbreaking studies on the mechanisms of germ line immortality (Ahmed, 2006; Ahmed and Hodgkin, 2000). Another important advantage of C. elegans is the ability to easily study in vivo phenomena such as age- or developmental stage–related differences in DNA repair capacity. For example, Clejan et al. (2006) showed that the error-prone DNA repair pathway of nonhomologous end joining has little or no role in the repair of DNA double-strand breaks in germ cells but is functional in somatic cells. Holway et al. (2006) showed that checkpoint silencing in response to DNA damage occurs in developing embryos but not in the germ line. Both these findings are important in our understanding developmental exposure to genotoxins in that they suggest a special protection for germ line cells.

DNA Damage–Related Pathological Processes in C. elegans

DNA damage–related pathological processes including carcinogenesis (He et al., 2007; Kroll, 2007; Pinkston-Gosse and Kenyon, 2007; Poulin et al., 2004; Sherwood et al., 2005; van Haaften et al., 2004a), aging (Antebi, 2007; Brys et al., 2007; Hartman et al., 1988; Johnson, 2003; Kenyon, 2005; Klass, 1977; Klass et al., 1983; Murakami, 2007; Rea et al., 2007; Ventura et al., 2006), and neurodegenerative diseases (described above) are also areas of active research in C. elegans. This research has both established the relevance of C. elegans as a model for the study of genotoxic agents (due to conservation of the DNA damage response) and enormously increased its utility in such studies by providing a wealth of complementary and contextual biological information related to the pathological responses to DNA damage in this organism.

Tools for the Study of DNA Damage, Repair, and Mutation in C. elegans

Caenorhabditis elegans is an excellent model for studies of genotoxicity due to the plethora of powerful tools available. Genetic manipulation via RNAi and generation of KOs or other mutants is relatively straightforward. If suitable mutants are not already available, they can be generated by a variety of approaches. These include untargeted and targeted methods, including chemical mutagenesis, transposon insertion, and biolistic transformation (Anderson, 1995; Barrett et al., 2004; Berezikov et al., 2004; Plasterk, 1995; Plasterk and Groenen, 1992; Rushforth et al., 1993).

Assays for the measurement of mutagenesis, DNA damage and repair, and transcriptional activity have also been developed for genotoxicity assessment in C. elegans (Table 5). Some DNA damage and repair assays in C. elegans can be carried out with as few as one or a few individual nematodes, permitting studies of interindividual differences and permitting high-throughput screening of DNA- damaging agents or genes involved in DNA repair. It is also possible, using PCR- or Southern blot–based methods, to distinguish damage and repair in different genomic regions and genomes (i.e., mitochondrial vs. nuclear DNA; (Hyun et al., 2008; Meyer et al., 2007)). Mutagenesis has been studied by a variety of methods (Table 5) including phenotype-based genetic mutation reversion screens, an out-of-frame LacZ transgene reporter, and direct sequencing.

TABLE 5
Genotoxicity Assays Available for the Caenorhabditis elegans Model

Genotoxin Studies in C. elegans

Unlike the case of neurotoxicology, there have so far been relatively few studies of genotoxicity per se using C. elegans. One exception has been the study of UV radiation, typically as a model genotoxin that introduces bulky DNA lesions (Astin et al., 2008; Coohill et al., 1988; Hartman, 1984; Hartman et al., 1988; Hyun et al., 2008; Jones and Hartman, 1996; Keller et al., 1987; Meyer et al., 2007; Stergiou et al., 2007; Stewart et al., 1991). However, other classes of genotoxins have been studied, including ionizing radiation (Dequen et al., 2005a; Johnson and Hartman, 1988; Stergiou et al., 2007; Weidhaas et al., 2006), heavy metals (Cui et al., 2007b; Neher and Sturzenbaum, 2006; Wang et al., 2008), methylmethanesulphonate (Holway et al., 2006), polycyclic aromatic hydrocarbons (Neher and Sturzenbaum, 2006), photosensitizers (Fujita et al., 1984; Hartman and Marshall, 1992; Mills and Hartman, 1998), and prooxidant compounds (Astin et al., 2008; Hartman et al., 2004; Hyun et al., 2008; Salinas et al., 2006). Studies have taken advantage of the utility of C. elegans as an in vivo model; for example, it was shown that nucleotide excision repair slowed in aging individuals (Meyer et al., 2007) and that longer lived and stress-resistant strains have faster nucleotide excision repair (Hyun et al., 2008) than do wild type. It has been possible to identify cases in which UV resistance was correlated to life span (Hyun et al., 2008; Murakami and Johnson, 1996), and others in which it was not (Hartman et al., 1988), so that theories about the relationship of DNA damage and repair with aging can be directly tested. Studies of aging populations or individuals are slow and expensive in mammalian models and impossible in vitro.

High-Throughput Approaches with C. elegans

High-throughput screening has two specific definitions in toxicology: (1) genome-wide screens for molecular targets or mediators of toxicity and (2) rapid, high-content chemical screens to detect potential toxicants. A genome-wide screen can serve as a hypothesis-finding tool, providing a direction for further mechanistic investigation. This approach is particularly useful for studying any toxicant with a poorly understood mechanism of action. Genome-wide screens can be done using forward genetics, DNA microarrays, or genome-wide RNAi in C. elegans.

High-throughput chemical screening, in comparison, has been proposed as a quicker and less expensive method for toxicity testing (Gibb, 2008). The conventional animal testing used by companies or agencies is labor intensive and time consuming, resulting in a large number of toxicants not being tested at all. It is estimated, for instance, that there are more than 10,000 environmental chemicals from several Environmental Protection Agency programs that require further testing (Dix et al., 2007). The objective of high-throughput chemical screening is to shortlist chemicals showing high toxicity, thereby setting priority for regulations as well as further toxicity testing in mammalian models.

High-throughput screening is feasible with C. elegans due to its experimental manipulability as well as several automation technologies. Caenorhabditis elegans is easy to handle in the laboratory; it can be cultivated on solid support or in liquid, in Petri dishes, tubes, or 6-, 12-, 24-, 96-, or 384-well plates. It can also be exposed to toxicants acutely or chronically by injection, feeding, or soaking. Automated imaging methods for absorbance, fluorescence, movement, or morphometric measurement have been developed since the late 1980s (Baek et al., 2002; Bennett and Pax, 1986; Hoshi and Shingai, 2006; Simonetta and Golombek, 2007; Tsibidis and Tavernarakis, 2007; Williams and Dusenbery, 1990b). Nowadays, cell sorters adapted to sort worms based on morphometric parameters or expression of fluorescent proteins combined with imaging platforms have been successfully used for large-scale promoter expression analyses and drug screening purposes (Burns et al., 2006; Dupuy et al., 2007; Pulak, 2006). Recently, a microfluidic C. elegans sorter with three dimensional subcellular imaging capabilities was developed, allowing high-throughput assays of higher complexity (Rohde et al., 2007).

While the simplicity and manipulability of the C. elegans system enables high-throughput approaches, it also leads to several potential disadvantages in toxicology studies. Caenorhabditis elegans exhibits important metabolic differences compared to vertebrates. For example, C. elegans is highly resistant to benzo[a]pyrene (Miller and Hartman, 1998), likely because it does not metabolize the chemical (M. Leung and J. Meyer, unpublished data). This problem can be potentially solved, however, by expressing the vertebrate cytochrome P450s in C. elegans. The impermeable cuticle layer as well as selective intestinal uptake, furthermore, may block the entry of chemicals, thereby necessitating high exposure doses to impact the worm's physiology. A mutant strain (dal-1) has recently been isolated that is healthy under laboratory conditions but exhibits altered intestinal morphology and increased intestinal absorption of a wide range of drugs (C. Paulson and J. Waddle, personal communication). The resultant-increased vulnerability of this strain to the toxic or pharmacological activities of tested compounds has the potential to increase the sensitivity of the C. elegans system.

Forward Genetics Screens in C. elegans

Forward genetics refers to the study of genes based on a given phenotype. In a forward genetics screen, C. elegans are treated with a mutagen, as described above. Mutant strains are then exposed to a toxicant and are screened for increased resistance or sensitivity. Once a resistant or hypersensitive mutant is identified, the mutation is located using two-point and three-point mapping and confirmed using single-gene rescue or RNAi phenocopying (Hodgkin and Hope, 1999). Forward genetics is efficient in C. elegans because the mutants can cover genes expressed in a variety of tissues. Caenorhabditis elegans is hermaphroditic, so homozygous mutant strains can be produced in the F2 generation via self-crossing.

Forward genetics screens are a useful method in mechanistic toxicology. Griffitts et al. (2001, 2005), for instance, discovered the role of glycolipid receptors and carbohydrate metabolism in Bacillus thuringiensis (Bt) toxins using C. elegans subjected to a forward genetics screen. The mutation of glycolipid receptors prevents Bt toxin from entering intestinal epithelium in C. elegans. Such a tissue-specific mechanism would have been difficult to detect using in vitro cell cultures.

Gene Expression Analysis in C. elegans

Caenorhabditis elegans has several advantages over other species in gene expression analysis. WormBase (Harris et al., 2004), the information-rich central genomic database of C. elegans, provides an intuitive interface into a well-annotated genome. Caenorhabditis elegans also has a consistent system of gene identification, thereby avoiding the confusion of gene identification that is common in many species, including human. The interactome modeling of C. elegans is also the most developed among all animal species (Dupuy et al., 2007; Li et al. 2004, 2008; Zhong and Sternberg, 2006) and along with other genome-level bioinformatics tools (Kim et al., 2001) greatly facilitates system-based analysis.

The results of gene expression analysis can be validated in vivo using mutational or transgenic approaches in C. elegans. For example, the gene expression of C. elegans exposed to ethanol, atrazine, polychlorinated biphenyls, endocrine disrupting chemicals, and polycyclic aromatic hydrocarbons have been profiled (Custodia et al., 2001; Kwon et al., 2004; Menzel et al., 2007; Reichert and Menzel, 2005). Follow-up studies with transgenic C. elegans expressing fluorescent markers were used to detect overexpression of protein in specific tissues in vivo (Menzel et al., 2007; Reichert and Menzel, 2005). Mutant C. elegans were also used to confirm the role of specific molecular targets based on gene expression analysis (Menzel et al., 2007).

Genome-Wide RNAi Screens in C. elegans

The discovery of RNAi mechanisms in C. elegans for which the 2006 Nobel Prize was awarded (Fire et al., 1998) and the complete sequencing of the nematode genome (C. elegans Sequencing Consortium, 1998) led to the generation of publically available RNAi libraries covering ~90% of its genes (Fewell and Schmitt, 2006; Kamath and Ahringer, 2003). Strategies to improve RNAi efficiency, especially in neurons, were further developed (Esposito et al., 2007; Lee et al., 2006; Simmer et al. 2002, 2003; Tabara et al., 2002; Tops et al., 2005). RNAi can be triggered by injection of worms with interfering double-strand RNA (dsRNA), by feeding them with transgenic bacteria producing the dsRNA or by soaking them in a solution of dsRNA. The latter allow timed RNAi exposure and genome-wide screens in 96- or 384-well plates with liquid worm cultures and have contributed to discoveries of mechanisms of axon guidance as well as mitochondrial involvement in oxidative stress and aging (Ayyadevara et al., 2007; Hamamichi et al., 2008; Hamilton et al., 2005; Ichishita et al., 2008; Lee et al., 2003; Schmitz et al., 2007; Zhang et al., 2006).

A genome-wide RNAi screen typically assesses a number of physiological parameters at the same time, such as viability, movement, food intake, and development, thereby facilitating the interpretation of screening results. While most RNAi screens have been done in wild-type C. elegans, some are performed using KO mutants to provide more sensitive or selective assays (Kaletta and Hengartner, 2006). Genome-wide RNAi screens are becoming a method of choice for discovering gene function. A recent study by Kim and Sun (2007), for example, identified a number of daf-2-dependent and nutrient-responsive genes that are responsive to paraquat-induced oxidative stress.

High-Content Chemical Screens

The use of C. elegans as a predictive model for human toxicity was first proposed in the context of heavy metals (Williams and Dusenbery, 1988). The C. elegans assay was validated as a predictor of mammalian acute lethality using eight different metal salts, generating LC50 values parallel to the rat and mouse LD50 values. A later study investigated the acute behavioral toxicity of 15 OP pesticides in C. elegans (Cole et al., 2004). The toxicity of these pesticides in C. elegans was found to be significantly correlated to the LD50 acute lethality values in rats and mice. Several other studies have also validated a number of C. elegans–based assays for predicting neurological and developmental toxicity in mammalian species (Anderson et al., 2004; Dhawan et al., 1999; Tatara et al., 1998; Williams et al., 2000).

A C. elegans–based, high-throughput toxicity screen was first published by the Freedman group at National Institute of Environmental Health Sciences (Peterson et al., in press); additional groups including industry and government groups in the United States and elsewhere are also carrying out high-throughput toxicity screening. Screens are typically conducted on a 96-well plate with a robotic liquid handling workstation (Biosort, Union Biometrica, Inc., Holliston, MA) to analyze the length, optical density, motion, and fluorescence of C. elegans. Caenorhabditis elegans is cultured in liquid from fertilized egg to adult through four distinct larval stages. The development, reproduction, and feeding behaviors of the C. elegans culture in response to different chemical exposures are characterized. The screen has been validated by the Freedman group with 60 chemicals including metals, pesticides, mutagens, and nontoxic agents (Peterson et al., in press).

The high-throughput toxicity screen is being further improved with additional genetics and automation techniques. The generalized stress response of C. elegans, for instance, was visualized with transgenic GFP constructs, providing a more sensitive end point for toxicity screens (Dengg and van Meel, 2004; Roh et al., 2006). Nematode locomotion can be tracked automatically, providing a more sensitive screen of neurotoxicity (Cole et al., 2004; Williams and Dusenbery, 1990b). Transgenic or mutant C. elegans can also be used in the high-throughput screen to detect specific modes of action, including metal response (Cioci et al., 2000), oxidative stress (Hasegawa et al., 2008; Leiers et al., 2003), and DNA damage (Denver et al., 2006). A microfluidic C. elegans sorter with three-dimensional subcellular imaging capabilities was recently reported, thereby allowing high-throughput assays of higher complexity (Rohde et al., 2007).

Environmental Assessment of Chemical Exposure

Nematodes are the most abundant animal in soil ecosystems and also found in aquatic and sediment environments. They serve many important roles in nutrient cycling and in maintaining environmental quality. These features have supported their use in ecotoxicological studies and, from the late 1970s, a variety of nematode species have been used to study environmental issues. During the late 1990s, C. elegans began to emerge as the nematode species of choice based on the tremendous body of knowledge developed by basic scientists using this model organism for biological studies. Although generally considered a soil organism, C. elegans lives in the interstitial water between soil particles and can be easily cultured within the laboratory in aquatic medium. The majority of environmental studies have been performed in an aquatic medium, given its ease of use, and as toxicological end points have been developed, the assessment tools have been applied to sediment and soil medium which allows for a more relevant direct environmental comparison.

The environmental toxicological literature using C. elegans is extensive and Table 6 provides an overview of laboratory-based studies where a toxicant of environmental interest has been added to a medium (water, sediment, or soil) followed by exposure to C. elegans and the assessment of an adverse effect. In a limited number of situations, C. elegans testing has been used to assess contamination in field settings (Table 7). Much of the early work explored metal toxicity and used lethality as an endpoint. Over time, a wider variety of toxicants have been tested and more sophisticated sublethal end points have been developed including the use of transgenic strains with specific biomarkers (Candido and Jones, 1996; Chu et al., 2005; Dengg and van Meel, 2004; Easton et al., 2001; Mutwakil et al., 1997; Roh et al., 2006), growth and reproduction (Anderson et al., 2001; Hoss and Weltje, 2007), feeding (Boyd et al., 2003), and movement (Anderson et al., 2004). These types of end points developed through environmental studies are directly applicable to the use of the organism as an alternative for mammalian testing.

TABLE 6
Representative Laboratory Studies Evaluating Environmentally Relevant Toxicants
TABLE 7
Examples of Field Studies Using Caenorhabditis elegans to Assess Environmental Samples

Two of the principal limitations in using C. elegans in environmental testing are concerns related to its comparison to other nematodes and reliable and simple methods for extracting them from soil and sediments. Given the almost countless variety of nematodes, it is impossible for one species to be representative of the entire Nematoda phylum. Limited studies comparing the toxicological effects between nematodes species indicate that C. elegans is as representative as any of the ones commonly used and, in many cases, little difference in response has been found between species (Boyd and Williams, 2003; Kammenga et al., 2000). Further, this organism is much more thoroughly understood and benefits from its ease of use.

Much progress has been made to develop better methods to extract the worm from soil and sediments. The initial method developed by Donkin and Dusenbery (1993) has led to a standardized soil toxicological testing method adopted in 2001 by the American Society for Testing and Materials (ASTM, 2002) and recently the International Standards Organization in Europe (ISO 2007). The initial extraction method has been improved through the use of transgenic strains of nematodes (Graves et al., 2005) which allows for GFP-labeled worms to be used that distinguishes the worms being tested in soils from the large numbers of indigenous species that are similar in size and appearance. It also makes easier removal from soil with high organic content. All this work has led to more interest in using C. elegans in environmental studies.

CONCLUSION: THE ROLE OF C. elegans IN TOXICOLOGY RESEARCH

The unique features of C. elegans make it an excellent model to complement mammalian models in toxicology research. Experiments with C. elegans do not incur the same costs as experiments with in vivo vertebrate models, while still permitting testing of hypotheses in an intact metazoan organism. The genetic tools available for C. elegans make it an excellent model for studying the roles of specific genes in toxicological processes and gene-environment interactions, while the life history of this organism lends itself to high-throughput analyses. Thus, C. elegans represents an excellent complement to in vitro or cell culture–based systems and in vivo vertebrate models.

FUNDING

National Institute of Environmental Health Sciences 10563; Department of Defense W81XWH-05-1-0239; the Gray E.B. Stahlman Chair of Neuroscience.

Supplementary Material

[Supplementary Data]

Acknowledgments

We thank Windy A. Boyd, Richard T. Di Giulio, and Jonathan H. Freedman for advice and assistance in the preparation of the manuscript.

References

  • Ahmed S. Uncoupling of pathways that promote postmitotic life span and apoptosis from replicative immortality of Caenorhabditis elegans germ cells. Aging Cell. 2006;5:559–563. [PubMed]
  • Ahmed S, Alpi A, Hengartner MO, Gartner A. C-elegans RAD-5/CLK-2 defines a new DNA damage checkpoint protein. Curr. Biol. 2001;11:1934–1944. [PubMed]
  • Ahmed S, Hodgkin J. MRT-2 checkpoint protein is required for germline immortality and telomere replication in C-elegans. Nature. 2000;403:159–164. [PubMed]
  • Anderson GL, Boyd WA, Williams PL. Assessment of sublethal endpoints for toxicity testing with the nematode Caenorhabditis elegans. Environ. Toxicol. Chem. 2001;20:833–838. [PubMed]
  • Anderson GL, Cole RD, Williams PL. Assessing behavioral toxicity with Caenorhabditis elegans. Environ. Toxicol. Chem. 2004;23:1235–1240. [PubMed]
  • Anderson JL, Head SI, Rae C, Morley JW. Brain function in Duchenne muscular dystrophy. Brain. 2002;125:4–13. [PubMed]
  • Anderson P. Mutagenesis. Methods Cell. Biol. 1995;48:31–58. [PubMed]
  • Antebi A. Genetics of aging in Caenorhabditis elegans. PLoS Genet. 2007;3:1565–1571. [PMC free article] [PubMed]
  • Antoshechkin I, Sternberg PW. The versatile worm: Genetic and genomic resources for Caenorhabditis elegans research. Nat. Rev. Genet. 2007;8:518–532. [PubMed]
  • Aoki H, Sato S, Takanami T, Ishihara T, Katsura I, Takahashi H, Higashitani A. Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans. Mol. Gen. Genet. 2000;264:119–126. [PubMed]
  • Astin JW, O'Neil NJ, Kuwabara PE. Nucleotide excision repair and the degradation of RNA pol II by the Caenorhabditis elegans XPA and Rsp5 orthologues, RAD-3 and WWP-1. DNA Repair. 2008;7:267–280. [PubMed]
  • American Society for Testing and Materials (ASTM) Annual Book of ASTM Standards, Vol. 11.05. 2002. Standard guide for conducting laboratory soil toxicity tests with the nematode Caenorhabditis elegans. E 2172-01. ASTM, West Conshohocken, PA. pp. 1606–1616.
  • Atchison WD. Effects of toxic environmental contaminants on voltage-gated calcium channel function: From past to present. J. Bioenerg. Biomembr. 2003;35:507–532. [PubMed]
  • Avery L, Horvitz HR. Effects of starvation and neuroactive drugs on feeding in Caenorhabditis elegans. J. Exp. Zool. 1990;253:263–270. [PubMed]
  • Ayyadevara S, Alla R, Thaden JJ, Shmookler Reis RJ. Remarkable longevity and stress resistance of nematode PI3K-null mutants. Aging Cell. 2008;7:13–22. [PubMed]
  • Ayyadevara S, Dandapat A, Singh SP, Siegel ER, Shmookler Reis RJ, Zimniak L, Zimniak P. Life span and stress resistance of Caenorhabditis elegans are differentially affected by glutathione transferases metabolizing 4-hydroxynon-2-enal. Mech. Ageing Dev. 2007;128:196–205. [PMC free article] [PubMed]
  • Ayyadevara S, Engle MR, Singh SP, Dandapat A, Lichti CF, Benes H, Shmookler Reis RJ, Liebau E, Zimniak P. Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal. Aging Cell. 2005;4:257–271. [PubMed]
  • Baek JH, Cosman P, Feng Z, Silver J, Schafer WR. Using machine vision to analyze and classify Caenorhabditis elegans behavioral phenotypes quantitatively. J. Neurosci. Methods. 2002;118:9–21. [PubMed]
  • Bardin PG, van Eeden SF, Moolman JA, Foden AP, Joubert JR. Organophosphate and carbamate poisoning. Arch. Intern. Med. 1994;154:1433–1441. [PubMed]
  • Bargmann CI. WormBook. 2006. Chemosensation in C. elegans. pp. 1–29. http://www.wormbook.org. [PubMed]
  • Barr MM, Garcia LR. WormBook. 2006. Male mating behavior; pp. 1–11. [PubMed]
  • Barrett PL, Fleming JT, Gobel V. Targeted gene alteration in Caenorhabditis elegans by gene conversion. Nat. Genet. 2004;36:1231–1237. [PubMed]
  • Barrows BD, Griffitts JS, Aroian RV. Caenorhabditis elegans carbohydrates in bacterial toxin resistance. Methods Enzymol. 2006;417:340–358. [PubMed]
  • Barrows BD, Griffitts JS, Aroian RV. Resistance is non-futile: Resistance to Cry5B in the nematode Caenorhabditis elegans. J. Invertebr. Pathol. 2007a;95:198–200. [PubMed]
  • Barrows BD, Haslam SM, Bischof LJ, Morris HR, Dell A, Aroian RV. Resistance to Bacillus thuringiensis toxin in Caenorhabditis elegans from loss of fucose. J. Biol. Chem. 2007b;282:3302–3311. [PubMed]
  • Bates EA, Victor M, Jones AK, Shi Y, Hart AC. Differential contributions of Caenorhabditis elegans histone deacetylases to huntingtin polyglutamine toxicity. J. Neurosci. 2006;26:2830–2838. [PubMed]
  • Baumeister R, Leimer U, Zweckbronner I, Jakubek C, Grunberg J, Haass C. Human presenilin-1, but not familial Alzheimer's disease (FAD) mutants, facilitate Caenorhabditis elegans Notch signalling independently of proteolytic processing. Genes Funct. 1997;1:149–159. [PubMed]
  • Benedetto A, Au C, Aschner M, Nass R. Manganese and C. elegans in Parkinson's disease. In: Nass R, Przedborski S, editors. Parkinson's Disease: Pathogenic and Therapeutic Insights from Toxin and Genetic Models. Elsevier Inc; 2008. (in press)
  • Bennett JL, Pax RA. Micromotility meter: An instrument designed to evaluate the action of drugs on motility of larval and adult nematodes. Parasitology. 1986;93(Pt 2):341–346. [PubMed]
  • Berezikov E, Bargmann CI, Plasterk RHA. Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Res. 2004;32:e40. [PMC free article] [PubMed]
  • Berezovska O, Frosch M, McLean P, Knowles R, Koo E, Kang D, Shen J, Lu FM, Lux SE, Tonegawa S, et al. The Alzheimer-related gene presenilin 1 facilitates notch 1 in primary mammalian neurons. Brain Res. 1999;69:273–280. [PubMed]
  • Berezovska O, Xia MQ, Hyman BT. Notch is expressed in adult brain, is coexpressed with presenilin-1, and is altered in Alzheimer disease. J. Neuropathol. Exp. Neurol. 1998;57:738–745. [PubMed]
  • Bessou C, Giugia JB, Franks CJ, Holden-Dye L, Segalat L. Mutations in the Caenorhabditis elegans dystrophin-like gene dys-1 lead to hyperactivity and suggest a link with cholinergic transmission. Neurogenetics. 1998;2:61–72. [PubMed]
  • Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson's disease. Nat. Neurosci. 2000;3:1301–1306. [PubMed]
  • Bezchlebova J, Cernohlavkova J, Lana J, Sochova I, Kobeticova K, Hofman J. Effects of toxaphene on soil organisms. Ecotoxicol. Environ. Saf. 2007;68:326–334. [PubMed]
  • Bharathi, Ravid R, Rao KS. Role of metals in neuronal apoptosis: Challenges associated with neurodegeneration. Curr. Alzheimer Res. 2006;3:311–326. [PubMed]
  • Bianchi L, Driscoll M. WormBook. 2006. Heterologous expression of C. elegans ion channels in Xenopus oocytes; pp. 1–16. [PubMed]
  • Black MC, Williams PL. Preliminary assessment of metal toxicity in the middle Tisza River (Hungary) flood plain. J Soils Sediments. 2001;1:203–206.
  • Blake DJ, Kroger S. The neurobiology of duchenne muscular dystrophy: Learning lessons from muscle? Trends Neurosci. 2000;23:92–99. [PubMed]
  • Boulton SJ, Gartner A, Reboul J, Vaglio P, Dyson N, Hill DE, Vidal M. Combined functional genomic maps of the C. elegans DNA damage response. Science. 2002;295:127–131. [PubMed]
  • Boulton SJ, Martin JS, Polanowska J, Hill DE, Gartner A, Vidal M. BRCA1/BARD1 orthologs required for DNA repair in Caenorhabditis elegans. Curr. Biol. 2004;14:33–39. [PubMed]
  • Boyd WA, Cole RD, Anderson GL, Williams PL. The effects of metals and food availability on the behavior of Caenorhabditis elegans. Environ. Toxicol. Chem. 2003;22:3049–3055. [PubMed]
  • Boyd WA, Williams PL. Comparison of the sensitivity of three nematode species to copper and their utility in aquatic and soil toxicity tests. Environ. Toxicol. Chem. 2003;22:2768–2774. [PubMed]
  • Boyd-Kimball D, Poon HF, Lynn BC, Cai J, Pierce WM, Jr, Klein JB, Ferguson J, Link CD, Butterfield DA. Proteomic identification of proteins specifically oxidized in Caenorhabditis elegans expressing human Abeta(1-42): Implications for Alzheimer's disease. Neurobiol. Aging. 2006;27:1239–1249. [PubMed]
  • Brandt R, Gergou A, Wacker I, Fath T, Hutter HA. Caenorhabditis elegans model of tau hyperphosphorylation: Induction of developmental defects by transgenic overexpression of Alzheimer's disease-like modified tau. Neurobiol. Aging. Accessed June 21. [PubMed]
  • Braungart E, Gerlach M, Riederer P, Baumeister R, Hoener MC. Caenorhabditis elegans MPP+ model of Parkinson's disease for high-throughput drug screenings. Neurodegener. Dis. 2004;1:175–183. [PubMed]
  • Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974;77:71–94. [PMC free article] [PubMed]
  • Brockie PJ, Maricq AV. WormBook. 2006. Ionotropic glutamate receptors: Genetics, behavior and electrophysiology; pp. 1–16. [PubMed]
  • Brys K, Vanfleteren JR, Braeckman BP. Testing the rate-of-living/oxidative damage theory of aging in the nematode model Caenorhabditis elegans. Exp. Gerontol. 2007;42:845–851. [PubMed]
  • Burmeister C, Luersen K, Heinick A, Hussein A, Domagalski M, Walter RD, Liebau E. Oxidative stress in Caenorhabditis elegans: Protective effects of the Omega class glutathione transferase (GSTO-1) FASEB J. 2008;22:343–354. [PubMed]
  • Burns AR, Kwok TC, Howard A, Houston E, Johanson K, Chan A, Cutler SR, McCourt P, Roy PJ. High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans. Nat. Protoc. 2006;1:1906–1914. [PubMed]
  • Bushnell DM, Martin ML. Quality of life and Parkinson's disease: Translation and validation of the US Parkinson's Disease Questionnaire (PDQ-39) Qual. Life. Res. 1999;8:345–350. [PubMed]
  • Candido EP, Jones D. Transgenic Caenorhabditis elegans strains as biosensors. Trends Biotechnol. 1996;14:125–129. [PubMed]
  • Cao S, Gelwix CC, Caldwell KA, Caldwell GA. Torsin-mediated protection from cellular stress in the dopaminergic neurons of Caenorhabditis elegans. J. Neurosci. 2005;25:3801–3812. [PubMed]
  • Carre-Pierrat M, Mariol MC, Chambonnier L, Laugraud A, Heskia F, Giacomotto J, Segalat L. Blocking of striated muscle degeneration by serotonin in C. elegans. J. Muscle Res. Cell Motil. 2006;27:253–258. [PubMed]
  • Caswell-Chen EP, Chen J, Lewis EE, Douhan GW, Nadler SA, Carey JR. Revising the standard wisdom of C. elegans natural history: Ecology of longevity. Sci. Aging Knowledge Environ. 2005;2005:pe30. [PMC free article] [PubMed]
  • C. elegans Sequencing Consortium. Genome sequence of the nematode C. elegans: A platform for investigating biology. Science. 1998;282:2012–2018. [PubMed]
  • Chase DL, Koelle MR. WormBook. 2007. Biogenic amine neurotransmitters in C. elegans; pp. 1–15. [PubMed]
  • Chen BL, Hall DH, Chklovskii DB. Wiring optimization can relate neuronal structure and function. Proc. Natl Acad. Sci. USA. 2006;103:4723–4728. [PMC free article] [PubMed]
  • Cheng Q, Valmas N, Reilly PE, Collins PJ, Kopittke R, Ebert PR. Caenorhabditis elegans mutants resistant to phosphine toxicity show increased longevity and cross-resistance to the synergistic action of oxygen. Toxicol. Sci. 2003;73:60–65. [PubMed]
  • Chiaki Fujitake MT, Inoue H, Takahashi K. In East Asia Worm Meeting. 2004. Characterization of C. elegans ubiquitin C-terminal hydrolases. June 28-July 1, 2004. p. 111.
  • Chisholm AD, Jin Y. Neuronal differentiation in C. elegans. Curr. Opin. Cell Biol. 2005;17:682–689. [PubMed]
  • Chopra VS, Metzler M, Rasper DM, Engqvist-Goldstein AE, Singaraja R, Gan L, Fichter KM, McCutcheon K, Drubin D, Nicholson DW, et al. HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin. Mamm. Genome. 2000;11:1006–1015. [PubMed]
  • Chu KW, Chan SKW, Chow KL. Improvement of heavy metal stress and toxicity assays by coupling a transgenic reporter in a mutant nematode strain. Aquat. Toxicol. 2005;74:320–332. [PubMed]
  • Cioci LK, Qiu L, Freedman JH. Transgenic strains of the nematode Caenorhabditis elegans as biomonitors of metal contamination. Environ. Toxicol. Chem. 2000;19:2122–2129.
  • Clejan I, Boerckel J, Ahmed S. Developmental modulation of nonhomologous end joining in Caenorhabditis elegans. Genetics. 2006;173:1301–1317. [PMC free article] [PubMed]
  • Cole RD, Anderson GL, Williams PL. The nematode Caenorhabditis elegans as a model of organophosphate-induced mammalian neurotoxicity. Toxicol. Appl. Pharmacol. 2004;194:248–256. [PubMed]
  • Conradt B, Xue D. WormBook. 2005. Programmed cell death; pp. 1–13. [PubMed]
  • Coohill T, Marshall T, Schubert W, Nelson G. Ultraviolet mutagenesis of radiation-sensitive (rad) mutants of the nematode Caenorhabditis elegans. Mutat. Res. 1988;209:99–106. [PubMed]
  • Cook A, Franks CJ, Holden-Dye L. WormBook. 2006. Electrophysiological recordings from the pharynx; pp. 1–7. [PubMed]
  • Cooper AA, Gitler AD, Cashikar A, Haynes CM, Hill KJ, Bhullar B, Liu K, Xu K, Strathearn KE, Liu F, et al. Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models. Science. 2006;313:324–328. [PMC free article] [PubMed]
  • Cui Y, Boyd WA, McBride SJ, Freedman JH. SOT Meeting March 25-29, 2007. 2007a. Toxicogenomic analysis of cadmium responsive transcription in Caenorhabditis elegans reveals novel genes and pathways involved in heavy metal resistance. Society of Toxicology, Charlotte, NC. p. 396.
  • Cui YX, McBride SJ, Boyd WA, Alper S, Freedman JH. Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity. Genome Biol. 2007b;8:R122. [PMC free article] [PubMed]
  • Custodia N, Won SJ, Novillo A, Wieland M, Li C, Callard IP. Caenorhabditis elegans as an environmental monitor using DNA microarray analysis. Environmental Hormones: the Scientific Basis of Endocrine Disruption. 2001;948:32–42. [PubMed]
  • Daitoku H, Fukamizu A. FOXO transcription factors in the regulatory networks of longevity. J. Biochem. 2007;141:769–774. [PubMed]
  • Dauer W, Przedborski S. Parkinson's disease: Mechanisms and models. Neuron. 2003;39:889–909. [PubMed]
  • Degtyareva NP, Greenwell P, Hofmann ER, Hengartner MO, Zhang L, Culotti JG, Petes TD. Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proc. Natl Acad. Sci. USA. 2002;99:2158–2163. [PMC free article] [PubMed]
  • Dengg M, van Meel JCA. Caenorhabditis elegans as model system for rapid toxicity assessment of pharmaceutical compounds. J. Pharmacol. Toxicol. Methods. 2004;50:209–214. [PubMed]
  • Denver DR, Feinberg S, Steding C, Durbin M, Lynch M. The relative roles of three DNA repair pathways in preventing Caenorhabditis elegan mutation accumulation. Genetics. 2006;174:57–65. [PMC free article] [PubMed]
  • Denver DR, Morris K, Lynch M, Thomas WK. High mutation rate and predominance of insertions in the Caenorhabditis elegans nuclear genome. Nature. 2004;430:679–682. [PubMed]
  • Denver DR, Morris K, Lynch M, Vassilieva LL, Thomas WK. High direct estimate of the mutation rate in the mitochondrial genome of Caenorhabditis elegans. Science. 2000;289:2342–2344. [PubMed]
  • Dequen F, Gagnon SN, Desnoyers S. Ionizing radiations in Caenorhabditis elegans induce poly (ADP-ribosyl) ation, a conserved DNA-damage response essential for survival. DNA Repair. 2005a;4:814–825. [PubMed]
  • Dequen F, St-Laurent JF, Gagnon SN, Carreau M, Desnoyers S. The Caenorhabditis elegans FancD2 ortholog is required for survival following DNA damage. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2005b;141:453–460. [PubMed]
  • Dhawan R, Dusenbery DB, Williams PL. Comparison of lethality, reproduction, and behavior as toxicological endpoints in the nematode Caenorhabditis elegans. J. Toxicol. Environ. Health A. 1999;58:451–462. [PubMed]
  • Dickey CA, Yue M, Lin WL, Dickson DW, Dunmore JH, Lee WC, Zehr C, West G, Cao S, Clark AM, et al. Deletion of the ubiquitin ligase CHIP leads to the accumulation, but not the aggregation, of both endogenous phospho- and caspase-3-cleaved tau species. J. Neurosci. 2006;26:6985–6996. [PubMed]
  • Dix DJ, Houck KA, Martin MT, Richard AM, Setzer RW, Kavlock RJ. The ToxCast program for prioritizing toxicity testing of environmental chemicals. Toxicol. Sci. 2007;95:5–12. [PubMed]
  • Donkin SG, Dusenberry DB. A soil toxicity test using the nematode Caenorhabditis elegans and an effective method of recovery. Arch. Environ. Contam. Toxicol. 1993;25:145–151.
  • Drake J, Link CD, Butterfield DA. Oxidative stress precedes fibrillar deposition of Alzheimer's disease amyloid beta-peptide (1-42) in a transgenic Caenorhabditis elegans model. Neurobiol. Aging. 2003;24:415–420. [PubMed]
  • Dupuy D, Bertin N, Hidalgo CA, Venkatesan K, Tu D, Lee D, Rosenberg J, Svrzikapa N, Blanc A, Carnec A, et al. Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans. Nat. Bio. technol. 2007;25:663–668. [PubMed]
  • Easton A, Guven K, de Pomerai DI. Toxicity of the dithiocarbamate fungicide mancozeb to the nontarget soil nematode, Caenorhabditis elegans. J. Biochem. Mol. Toxicol. 2001;15:15–25. [PubMed]
  • Esposito G, Di Schiavi E, Bergamasco C, Bazzicalupo P. Efficient and cell specific knock-down of gene function in targeted C. elegans neurons. Gene. 2007;395:170–176. [PubMed]
  • Faber PW, Alter JR, MacDonald ME, Hart AC. Polyglutamine-mediated dysfunction and apoptotic death of a Caenorhabditis elegans sensory neuron. Proc. Natl Acad. Sci. USA. 1999;96:179–184. [PMC free article] [PubMed]
  • Faber PW, Voisine C, King DC, Bates EA, Hart AC. Glutamine/proline-rich PQE-1 proteins protect Caenorhabditis elegans neurons from huntingtin polyglutamine neurotoxicity. Proc. Natl Acad. Sci. USA. 2002;99:17131–17136. [PMC free article] [PubMed]
  • Fewell GD, Schmitt K. Vector-based RNAi approaches for stable, inducible and genome-wide screens. Drug Discovery Today. 2006;11:975–982. [PubMed]
  • Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998;391:806–811. [PubMed]
  • Florez-McClure ML, Hohsfield LA, Fonte G, Bealor MT, Link CD. Decreased insulin-receptor signaling promotes the autophagic degradation of beta-amyloid peptide in C. elegans. Autophagy. 2007;3:569–580. [PubMed]
  • Fonte V, Kipp DR, Yerg J, III, Merin D, Forrestal M, Wagner E, Roberts CM, Link CD. Suppression of in vivo beta-amyloid peptide toxicity by overexpression of the HSP-16.2 small chaperone protein. J. Biol. Chem. 2008;283:784–791. [PubMed]
  • Francis R, McGrath G, Zhang J, Ruddy DA, Sym M, Apfeld J, Nicoll M, Maxwell M, Hai B, Ellis MC, et al. aph-1 and pen-2 are required for Notch pathway signaling, gamma-secretase cleavage of betaAPP, and presenilin protein accumulation. Dev. Cell. 2002;3:85–97. [PubMed]
  • Freedman JH, Chen M, Coughlan S, Boyd WA. SOT Meeting March 5-9, 2006, (Abstract No. 1178). 2006. Changes in gene expression associated with exposure to environmental toxicants. Society of Toxicology, San Diego, CA. p. 240.
  • Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. DNA Repair and Mutagenesis. Washington, DC: ASM Press; 2006.
  • Fujii M, Matsumoto Y, Tanaka N, Miki K, Suzuki T, Ishii N, Ayusawa D. Mutations in chemosensory cilia cause resistance to paraquat in nematode Caenorhabditis elegans. J. Biol. Chem. 2004;279:20277–20282. [PubMed]
  • Fujii M, Tanaka N, Miki K, Hossain MN, Endoh M, Ayusawa D. Uncoupling of longevity and paraquat resistance in mutants of the nematode Caenorhabditis elegans. Biosci. Biotechnol. Biochem. 2005;69:2015–2018. [PubMed]
  • Fujita H, Ishii N, Suzuki K. Effects of 8-methoxypsoralen plus near-ultraviolet light on the nematode Caenorhabditis-elegans. Photochem. Photobiol. 1984;39:831–834. [PubMed]
  • Gabel CV, Antonie F, Chuang CF, Samuel AD, Chang C. Distinct cellular and molecular mechanisms mediate initial axon development and adult-stage axon regeneration in C. elegans. Development. 2008;135:1129–1136. [PubMed]
  • Gagnon SN, Hengartner MO, Desnoyers S. The genes pme-1 and pme-2 encode two poly(ADP-ribose) polymerases in Caenorhabditis elegans. Biochem. J. 2002;368:263–271. [PMC free article] [PubMed]
  • Gami MS, Iser WB, Hanselman KB, Wolkow CA. Activated AKT/PKB signaling in C. elegans uncouples temporally distinct outputs of DAF-2/insulin-like signaling. BMC Dev. Biol. 2006;6:45. [PMC free article] [PubMed]
  • Gao HM, Liu B, Hong JS. Critical role for microglial NADPH oxidase in rotenone-induced degeneration of dopaminergic neurons. J. Neurosci. 2003;23:6181–6187. [PubMed]
  • Gartner A, Milstein S, Ahmed S, Hodgkin J, Hengartner MO. A conserved checkpoint pathway mediates DNA damage-induced apoptosis and cell cycle arrest in C. elegans. Mol. Cell. 2000;5:435–443. [PubMed]
  • Garza A, Vega R, Soto E. Cellular mechanisms of lead neurotoxicity. Med. Sci. Monit. 2006;12:RA57–RA65. [PubMed]
  • Gaud A, Simon JM, Witzel T, Carre-Pierrat M, Wermuth CG, Segalat L. Prednisone reduces muscle degeneration in dystrophin-deficient Caenorhabditis elegans. Neuromuscul. Disord. 2004;14:365–370. [PubMed]
  • Gibb S. Toxicity testing in the 21st century: A vision and a strategy. Reprod. Toxicol. 2008;25:136–138. [PubMed]
  • Gieseler K, Abdel-Dayem M, Segalat L. In vitro interactions of Caenorhabditis elegans dystrophin with dystrobrevin and syntrophin. FEBS Lett. 1999a;461:59–62. [PubMed]
  • Gieseler K, Bessou C, Segalat L. Dystrobrevin- and dystrophin-like mutants display similar phenotypes in the nematode Caenorhabditis elegans. Neurogenetics. 1999b;2:87–90. [PubMed]
  • Gieseler K, Grisoni K, Mariol MC, Segalat L. Overexpression of dystrobrevin delays locomotion defects and muscle degeneration in a dystrophin-deficient Caenorhabditis elegans. Neuromuscul. Disord. 2002;12:371–377. [PubMed]
  • Gieseler K, Grisoni K, Segalat L. Genetic suppression of phenotypes arising from mutations in dystrophin-related genes in Caenorhabditis elegans. Curr. Biol. 2000;10:1092–1097. [PubMed]
  • Gieseler K, Mariol MC, Bessou C, Migaud M, Franks CJ, Holden-Dye L, Segalat L. Molecular, genetic and physiological characterisation of dystrobrevin-like (dyb-1) mutants of Caenorhabditis elegans. J. Mol. Biol. 2001;307:107–117. [PubMed]
  • Gitler AD, Bevis BJ, Shorter J, Strathearn KE, Hamamichi S, Su LJ, Caldwell KA, Caldwell GA, Rochet JC, McCaffery JM, et al. The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis. Proc. Natl Acad. Sci. USA. 2008;105:145–150. [PMC free article] [PubMed]
  • Goldstein P, Modric T. Transgenerational, ultrastructural analysis on the antioxidative effects of tocopherol on early gametogenesis in Caenorhabditis elegans grown in 100% oxygen. Toxicol. Appl. Pharmacol. 1994;124:212–220. [PubMed]
  • Goncalves PP, Silva VS. Does neurotransmission impairment accompany aluminium neurotoxicity? J. Inorg. Biochem. 2007;101:1291–1338. [PubMed]
  • Goodman MB. WormBook. 2006. Mechanosensation; pp. 1–14. [PMC free article] [PubMed]
  • Gorell JM, Johnson CC, Rybicki BA, Peterson EL, Richardson RJ. The risk of Parkinson's disease with exposure to pesticides, farming, well water, and rural living. Neurology. 1998;50:1346–1350. [PubMed]
  • Grad LI, Lemire BD. Mitochondrial complex I mutations in Caenorhabditis elegans produce cytochrome C oxidase deficiency, oxidative stress and vitamin-responsive lactic acidosis. Hum. Mol. Genet. 2004;13:303–314. [PubMed]
  • Graves AL, Boyd WA, Williams PL. Using transgenic Caenorhabditis elegans in soil toxicity testing. Arch. Environ. Contam. Toxicol. 2005;48:490–494. [PubMed]
  • Greenwald IS, Horvitz HR. Unc-93(E1500)—A behavioral mutant of Caenorhabditis-elegans that defines a gene with a wild-type null phenotype. Genetics. 1980;96:147–164. [PMC free article] [PubMed]
  • Griffitts JS, Haslam SM, Yang T, Garczynski SF, Mulloy B, Morris H, Cremer PS, Dell A, Adang MJ, Aroian RV. Glycolipids as receptors for Bacillus thuringiensis crystal toxin. Science. 2005;307:922–925. [PubMed]
  • Griffitts JS, Huffman DL, Whitacre JL, Barrows BD, Marroquin LD, Muller R, Brown JR, Hennet T, Esko JD, Aroian RV. Resistance to a bacterial toxin is mediated by removal of a conserved glycosylation pathway required for toxin-host interactions. J. Biol. Chem. 2003;278:45594–45602. [PubMed]
  • Griffitts JS, Whitacre JL, Stevens DE, Aroian RV. Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme. Science. 2001;293:860–864. [PubMed]
  • Grisoni K, Gieseler K, Mariol MC, Martin E, Carre-Pierrat M, Moulder G, Barstead R, Segalat L. The stn-1 syntrophin gene of C. elegans is functionally related to dystrophin and dystrobrevin. J. Mol. Biol. 2003;332:1037–1046. [PubMed]
  • Grisoni K, Gieseler K, Segalat L. Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans. Eur. J. Biochem. 2002a;269:1607–1612. [PubMed]
  • Grisoni K, Martin E, Gieseler K, Mariol MC, Segalat L. Genetic evidence for a dystrophin-glycoprotein complex (DGC) in Caenorhabditis elegans. Gene. 2002b;294:77–86. [PubMed]
  • Guo J, Lemire BD. The ubiquinone-binding site of the Saccharomyces cerevisiae succinate-ubiquinone oxidoreductase is a source of superoxide. J. Biol. Chem. 2003;278:47629–47635. [PubMed]
  • Gutierrez-Zepeda A, Luo Y. Testing the amyloid toxicity hypothesis of Alzheimer's disease in transgenic Caenorhabditis elegans model. Front. Biosci. 2004;9:3333–3338. [PubMed]
  • Gutierrez-Zepeda A, Santell R, Wu Z, Brown M, Wu Y, Khan I, Link CD, Zhao B, Luo Y. Soy isoflavone glycitein protects against beta amyloid-induced toxicity and oxidative stress in transgenic Caenorhabditis elegans. BMC Neurosci. 2005;6:54. [PMC free article] [PubMed]
  • Guven K, Power RS, Avramides S, Allender R, de Pomerai DI. The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain of Caenorhabditis elegans. J. Biochem. Mol. Toxicol. 1999;13:324–333. [PubMed]
  • Hamamichi S, Rivas RN, Knight AL, Cao S, Caldwell KA, Caldwell GA. Hypothesis-based RNAi screening identifies neuroprotective genes in a Parkinson's disease model. Proc. Natl Acad. Sci. USA. 2008;105:728–733. [PMC free article] [PubMed]
  • Hamilton B, Dong Y, Shindo M, Liu W, Odell I, Ruvkun G, Lee SS. A systematic RNAi screen for longevity genes in C. elegans. Genes Dev. 2005;19:1544–1555. [PMC free article] [PubMed]
  • Harris TW, Chen NS, Cunningham F, Tello-Ruiz M, Antoshechkin I, Bastiani C, Bieri T, Blasiar D, Bradnam K, Chan J, et al. WormBase: A multi-species resource for nematode biology and genomics. Nucleic Acids Res. 2004;32:D411–D417. [PMC free article] [PubMed]
  • Hartman P, Childress E, Beyer T. Nematode development is inhibited by methyl viologen and high oxygen concentrations at a rate inversely proportional to life span. J. Gerontol. A Biol. Sci. Med. Sci. 1995;50:B322–B6. [PubMed]
  • Hartman P, Ponder R, Lo HH, Ishii N. Mitochondrial oxidative stress can lead to nuclear hypermutability. Mech. Ageing Dev. 2004;125:417–420. [PubMed]
  • Hartman PS. UV irradiation of wild-type and radiation-sensitive mutants of the nematode Caenorhabditis elegans—Fertilities, survival, and parental effects. Photochem. Photobiol. 1984;39:169–175. [PubMed]
  • Hartman PS. Epistatic interactions of radiation-sensitive (Rad) mutants of Caenorhabditis elegans. Genetics. 1985;109:81–93. [PMC free article] [PubMed]
  • Hartman PS, Herman RK. Radiation-sensitive mutants of Caenorhabditis elegans. Genetics. 1982;102:159–178. [PMC free article] [PubMed]
  • Hartman PS, Hevelone J, Dwarakanath V, Mitchell DL. Excision repair of UV radiation-induced DNA damage in Caenorhabditis elegans. Genetics. 1989;122:379–385. [PMC free article] [PubMed]
  • Hartman PS, Marshall A. Inactivation of wild-type and rad mutant Caenorhabditis elegans by 8-methoxypsoralen and near ultraviolet radiation. Photochem. Photobiol. 1992;55:103–111. [PubMed]
  • Hartman PS, Nelson GA. Processing of DNA damage in the nematode Caenorhabditis elegans. In: Nickoloff JA, Hoekstra MF, editors. DNA damage and Repair Volume 1: DNA Repair in Prokaryotes and Lower Eukaryotes. Vol. 1. Totowa, NJ: Humana Press; 1998. pp. 557–576.
  • Hartman PS, Simpson VJ, Johnson T, Mitchell D. Radiation sensitivity and DNA repair in Caenorhabditis elegans strains with different mean life spans. Mutat. Res. 1988;208:77–82. [PubMed]
  • Hasegawa A, van der Bliek AM. Inverse correlation between expression of the Wolfs Hirschhorn candidate gene Letm1 and mitochondrial volume in C. elegans and in mammalian cells. Hum. Mol. Genet. 2007;16:2061–2071. [PubMed]
  • Hasegawa K, Miwa S, Isomura K, Tsutsumiuchi K, Taniguchi H, Miwa J. Acrylamide-responsive genes in the nematode Caenorhabditis elegans. Toxicol. Sci. 2008;101:215–225. [PubMed]
  • Hasshoff M, Bohnisch C, Tonn D, Hasert B, Schulenburg H. The role of Caenorhabditis elegans insulin-like signaling in the behavioral avoidance of pathogenic Bacillus thuringiensis. FASEB J. 2007;21:1801–1812. [PubMed]
  • He L, He XY, Lowe SW, Hannon GJ. microRNAs join the p53 network. Another piece in the tumour-suppression puzzle. Nat. Rev. Cancer. 2007;7:819–822. [PMC free article] [PubMed]
  • Hengartner MO, Horvitz HR. Programmed cell death in Caenorhabditis elegans. Curr. Opin. Genet. Dev. 1994;4:581–586. [PubMed]
  • Hevelone J, Hartman PS. An endonuclease from Caenorhabditis elegans. Partial purification and characterization. Biochem. Genet. 1988;26:447–461. [PubMed]
  • Hilliard MA, Apicella AJ, Kerr R, Suzuki H, Bazzicalupo P, Schafer WR. In vivo imaging of C. elegans ASH neurons: Cellular response and adaptation to chemical repellents. EMBO J. 2005;24:63–72. [PMC free article] [PubMed]
  • Hills T, Brockie PJ, Maricq AV. Dopamine and glutamate control area-restricted search behavior in Caenorhabditis elegans. J. Neurosci. 2004;24:1217–1225. [PubMed]
  • Hirata Y. Manganese-induced apoptosis in PC12 cells. Neurotoxicol. Teratol. 2002;24:639–653. [PubMed]
  • Hitchcock DR, Black MC, Williams PL. Investigations into using the nematode Caenorhabditis elegans for municipal and industrial wastewater toxicity testing. Arch. Environ. Contam. Toxicol. 1997;33:252–260. [PubMed]
  • Hobert O. WormBook. 2005. Specification of the nervous system; pp. 1–19. [PubMed]
  • Hodgkin J, Hope IA. Practical Approach Series; C. elegans: A Practical Approach. 1999. Conventional genetics; pp. 245–270.
  • Holbert S, Dedeoglu A, Humbert S, Saudou F, Ferrante RJ, Neri C. Cdc42-interacting protein 4 binds to huntingtin: Neuropathologic and biological evidence for a role in Huntington's disease. Proc. Natl Acad. Sci. USA. 2003;100:2712–2717. [PMC free article] [PubMed]
  • Holway AH, Kim SH, La Volpe A, Michael WM. Checkpoint silencing during the DNA damage response in Caenorhabditis elegans embryos. J. Cell Biol. 2006;172:999–1008. [PMC free article] [PubMed]
  • Honda S, Ishii N, Suzuki K, Matsuo M. Oxygen-dependent perturbation of life span and aging rate in the nematode. J. Gerontol. 1993;48:B57–B61. [PubMed]
  • Hope IA. Background on Caenorhabditis elegans. In: Hope IA, editor. C. elegans: A Practical Approach. NY: Oxford University Press; 1999. pp. 1–15.
  • Horvitz HR. Worms, life, and death (Nobel lecture) Chem. biochem. 2003;4:697–711. [PubMed]
  • Hoshi K, Shingai R. Computer-driven automatic identification of locomotion states in Caenorhabditis elegans. J. Neurosci. Methods. 2006;157:355–363. [PubMed]
  • Hoss S, Arndt M, Baumgarte S, Tebbe CC, Nguyen HT, Jehle JA. Effects of transgenic corn and Cry1Ab protein on the nematode, Caenorhabditis elegans. Ecotoxicol. Environ. Saf. 2008;70:334–340. [PubMed]
  • Hoss S, Haitzer M, Traunspurger W, Gratzer H, Ahlf W, Steinberg C. Influence of particle size distribution and content of organic matter on the toxicity of copper in sediment bioassays using Caenorhabditis elegans (nematoda) Water Air Soil Pollut. 1997;99:689–695.
  • Hoss S, Haitzer M, Traunspurger W, Steinberg CEW. Growth and fertility of Caenorhabditis elegans (Nematoda) in unpolluted freshwater sediments: Response to particle size distribution and organic content. Environ. Toxicol. Chem. 1999;18:2921–2925.
  • Hoss S, Henschel T, Haitzer M, Traunspurger W, Steinberg CE. Toxicity of cadmium to Caenorhabditis elegans (Nematoda) in whole sediment and pore water—The ambiguous role of organic matter. Environ. Toxicol. Chem. 2001;20:2794–2801. [PubMed]
  • Hoss S, Weltje L. Endocrine disruption in nematodes: Effects and mechanisms. Ecotoxicology. 2007;16:15–28. [PubMed]
  • Houthoofd K, Braeckman BP, Lenaerts I, Brys K, De Vreese A, Van Eygen S, Vanfleteren JR. Ageing is reversed, and metabolism is reset to young levels in recovering dauer larvae of C. elegans. Exp. Gerontol. 2002;37:1015–1021. [PubMed]
  • Huffman DL, Abrami L, Sasik R, Corbeil J, van der Goot FG, Aroian RV. Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins. Proc. Natl Acad. Sci. USA. 2004a;101:10995–11000. [PMC free article] [PubMed]
  • Huffman DL, Bischof LJ, Griffitts JS, Aroian RV. Pore worms: Using Caenorhabditis elegans to study how bacterial toxins interact with their target host. Int. J. Med. Microbiol. 2004b;293:599–607. [PubMed]
  • Hyun M, Lee J, Lee K, May A, Bohr VA, Ahn B. Longevity and resistance to stress correlate with DNA repair capacity in Caenorhabditis elegans. Nucleic Acids Res. 2008;36:1380–1389. [PMC free article] [PubMed]
  • Ibiam U, Grant A. RNA/DNA ratios as a sublethal endpoint for large-scale toxicity tests with the nematode Caenorhabditis elegans. Environ. Toxicol. Chem. 2005;24:1155–1159. [PubMed]
  • Ichibangase T, Saimaru H, Takamura N, Kuwahara T, Koyama A, Iwatsubo T, Imai K. Proteomics of Caenorhabditis elegans over-expressing human alpha-synuclein analyzed by fluorogenic derivatization-liquid chromatography/tandem mass spectrometry: Identification of actin and several ribosomal proteins as negative markers at early Parkinson's disease stages. Biomed. Chromatogr. 2008;22:232–234. [PubMed]
  • Ichishita R, Tanaka K, Sugiura Y, Sayano T, Mihara K, Oka T. An RNAi screen for mitochondrial proteins required to maintain the morphology of the organelle in Caenorhabditis elegans. J. Biochem. 2008;143:449–454. [PubMed]
  • Inoue H, Hisamoto N, An JH, Oliveira RP, Nishida E, Blackwell TK, Matsumoto K. The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response. Genes Dev. 2005;19:2278–2283. [PMC free article] [PubMed]
  • Inoue T, Thomas JH. Suppressors of transforming growth factor-beta pathway mutants in the Caenorhabditis elegans dauer formation pathway. Genetics. 2000;156:1035–1046. [PMC free article] [PubMed]
  • Ishiguro H, Yasuda K, Ishii N, Ihara K, Ohkubo T, Hiyoshi M, Ono K, Senoo-Matsuda N, Shinohara O, Yosshii F, et al. Enhancement of oxidative damage to cultured cells and Caenorhabditis elegans by mitochondrial electron transport inhibitors. IUBMB life. 2001;51:263–268. [PubMed]
  • Ishii N, Suzuki N, Hartman PS, Suzuki K. The radiation-sensitive mutant rad-8 of Caenorhabditis elegans is hypersensitive to the effects of oxygen on aging and development. Mech. Ageing Dev. 1993;68:1–10. [PubMed]
  • Ishii N, Takahashi K, Tomita S, Keino T, Honda S, Yoshino K, Suzuki K. A methyl viologen-sensitive mutant of the nematode Caenorhabditis elegans. Mutat. Res. 1990;237:165–171. [PubMed]
  • International Standards Organization (ISO) 2007 ISO/CD 10872 Water quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda). Geneva, Switzerland.
  • Jackson D, Lewis J, Anderson S, Gehman E, Szilagyi M, Clegg E. SOT Meeting March 5-9, 2006. 2006. A nematode model to elucidate mechanisms of developmental toxicity. Society of Toxicology, San Diego, CA. p. 229.
  • Jagasia R, Grote P, Westermann B, Conradt B. DRP-1-mediated mitochondrial fragmentation during EGL-1-induced cell death in C. elegans. Nature. 2005;433:754–760. [PubMed]
  • Jiang GC, Tidwell K, McLaughlin BA, Cai J, Gupta RC, Milatovic D, Nass R, Aschner M. Neurotoxic potential of depleted uranium effects in primary cortical neuron cultures and in Caenorhabditis elegans. Toxicol. Sci. 2007;99:553–565. [PubMed]
  • Jin Y. Synaptogenesis: Insights from worm and fly. Curr. Opin. Neurobiol. 2002;12:71–79. [PubMed]
  • Jin Y. WormBook. 2005. Synaptogenesis; pp. 1–11. [PubMed]
  • Johnson TE. Advantages and disadvantages of Caenorhabditis elegans for aging research. Exp. Gerontol. 2003;38:1329–1332. [PubMed]
  • Johnson TE, Hartman PS. Radiation effects on lifespan in Caenorhabditis elegans. J. Gerontol. 1988;43:B137–B141. [PubMed]
  • Johnson TE, Nelson GA. Caenorhabditis elegans: A model system for space biology studies. Exp. Gerontol. 1991;26:299–309. [PubMed]
  • Jones CA, Hartman PS. Replication in UV-irradiated Caenorhabditis elegans embryos. Photochem. Photobiol. 1996;63:187–192. [PubMed]
  • Jones D, Candido EP. Feeding is inhibited by sublethal concentrations of toxicants and by heat stress in the nematode Caenorhabditis elegans: Relationship to the cellular stress response. J. Exp. Zool. 1999;284:147–157. [PubMed]
  • Jones D, Stringham EG, Babich SL, Candido EP. Transgenic strains of the nematode C. elegans in biomonitoring and toxicology: Effects of captan and related compounds on the stress response. Toxicology. 1996;109:119–127. [PubMed]
  • Jorgensen EM. WormBook. 2005. Gaba; pp. 1–13. [PubMed]
  • Kaletta T, Hengartner MO. Finding function in novel targets: C-elegans as a model organism. Nat. Rev. Drug Discovery. 2006;5:387–398. [PubMed]
  • Kamath RS, Ahringer J. Genome-wide RNAi screening in Caenorhabditis elegans. Methods. 2003;30:313–321. [PubMed]
  • Kammenga JE, Dallinger R, Donker MH, Kohler HR, Simonsen V, Triebskorn R, Weeks JM. Biomarkers in terrestrial invertebrates for ecotoxicological soil risk assessment. Rev. Environ. Contam. Toxicol. 2000;164:93–147. [PubMed]
  • Kanugula S, Pegg AE. Novel DNA repair alkyltransferase from Caenorhabditis elegans. Environ. Mol. Mutagen. 2001;38:235–243. [PubMed]
  • Kell A, Ventura N, Kahn N, Johnson TE. Activation of SKN-1 by novel kinases in Caenorhabditis elegans. Free Radic. Biol. Med. 2007;43:1560–1566. [PMC free article] [PubMed]
  • Keller CI, Calkins J, Hartman PS, Rupert CS. UV photobiology of the nematode Caenorhabditis-elegans—Action spectra, absence of photoreactivation and effects of caffeine. Photochem. Photobiol. 1987;46:483–488. [PubMed]
  • Kelly KO, Dernburg AF, Stanfield GM, Villeneuve AM. Caenorhabditis elegans msh-5 is required for both normal and radiation-induced meiotic crossing over but not for completion of meiosis. Genetics. 2000;156:617–630. [PMC free article] [PubMed]
  • Kenyon C. The plasticity of aging: Insights from long-lived mutants. Cell. 2005;120:449–460. [PubMed]
  • Kim SK, Lund J, Kiraly M, Duke K, Jiang M, Stuart JM, Eizinger A, Wylie BN, Davidson GS. A gene expression map for Caenorhabditis elegans. Science. 2001;293:2087–2092. [PubMed]
  • Kim Y, Sun H. Functional genomic approach to identify novel genes involved in the regulation of oxidative stress resistance and animal lifespan. Aging Cell. 2007;6:489–503. [PubMed]
  • Kinchen JM, Hengartner MO. Tales of cannibalism, suicide, and murder: Programmed cell death in C. elegans. Curr. Top. Dev. Biol. 2005;65:1–45. [PubMed]
  • Kiontke K, Sudhaus W. WormBook. 2006. Ecology of Caenorhabditis species; pp. 1–14. [PubMed]
  • Kipreos ET. WormBook. 2005. Ubiquitin-mediated pathways in C. elegans; pp. 1–24. [PubMed]
  • Kitagawa N, Shimohama S, Oeda T, Uemura K, Kohno R, Kuzuya A, Shibasaki H, Ishii N. The role of the presenilin-1 homologue gene sel-12 of Caenorhabditis elegans in apoptotic activities. J. Biol. Chem. 2003;278:12130–12134. [PubMed]
  • Klass M, Nguyen PN, Dechavigny A. Age-correlated changes in the DNA template in the nematode Caenorhabditis elegans. Mech. Ageing Dev. 1983;22:253–263. [PubMed]
  • Klass MR. Aging in nematode Caenorhabditis elegans—Major biological and environmental factors influencing lifespan. Mech. Ageing Dev. 1977;6:413–429. [PubMed]
  • Koh JY. Zinc and disease of the brain. Mol. Neurobiol. 2001;24:99–106. [PubMed]
  • Kondo M, Senoo-Matsuda N, Yanase S, Ishii T, Hartman PS, Ishii N. Effect of oxidative stress on translocation of DAF-16 in oxygen-sensitive mutants, mev-1 and gas-1 of Caenorhabditis elegans. Mech. Ageing Dev. 2005;126:637–641. [PubMed]
  • Kopin IJ, Markey SP. MPTP toxicity: Implications for research in Parkinson's disease. Annu. Rev. Neurosci. 1988;11:81–96. [PubMed]
  • Koselke L, Sam C, Hajela R, Atchison B. SOT Meeting March 25-29, 2007 (Abstract No. 98). 2007. Protective effects of verapamil on mercury toxicity in C. elegans. Society of Toxicology, Charlotte, NC. p. 20.
  • Kraemer BC, Burgess JK, Chen JH, Thomas JH, Schellenberg GD. Molecular pathways that influence human tau-induced pathology in Caenorhabditis elegans. Hum. Mol. Genet. 2006;15:1483–1496. [PubMed]
  • Kraemer BC, Schellenberg GD. SUT-1 enables tau-induced neurotoxicity in C. elegans. Hum. Mol. Genet. 2007;16:1959–1971. [PubMed]
  • Kraemer BC, Zhang B, Leverenz JB, Thomas JH, Trojanowski JQ, Schellenberg GD. Neurodegeneration and defective neurotransmission in a Caenorhabditis elegans model of tauopathy. Proc. Natl Acad. Sci. USA. 2003;100:9980–9985. [PMC free article] [PubMed]
  • Kroll J. Molecular chaperones and the epigenetics of longevity and cancer resistance. Ann. N. Y. Acad. Sci. 2007;1100:75–83. [PubMed]
  • Kurz CL, Shapira M, Chen K, Baillie DL, Tan MW. Caenorhabditis elegans pgp-5 is involved in resistance to bacterial infection and heavy metal and its regulation requires TIR-1 and a p38 map kinase cascade. Biochem. Biophys. Res. Commun. 2007;363:438–443. [PMC free article] [PubMed]
  • Kuwahara T, Koyama A, Gengyo-Ando K, Masuda M, Kowa H, Tsunoda M, Mitani S, Iwatsubo T. Familial Parkinson mutant alpha-synuclein causes dopamine neuron dysfunction in transgenic Caenorhabditis elegans. J. Biol. Chem. 2006;281:334–340. [PubMed]
  • Kwon JY, Hong M, Choi MS, Kang SJ, Duke K, Kim S, Lee SH, Lee JH. Ethanol-response genes and their regulation analyzed by a microarray and comparative genomic approach in the nematode Caenorhabditis elegans. Genomics. 2004;83:600–614. [PubMed]
  • Lagido C, Pettitt J, Porter AJR, Paton GI, Glover LA. Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEBS Lett. 2001;493:36–39. [PubMed]
  • Lakso M, Vartiainen S, Moilanen AM, Sirvio J, Thomas JH, Nass R, Blakely RD, Wong G. Dopaminergic neuronal loss and motor deficits in Caenorhabditis elegans overexpressing human alpha-synuclein. J. Neurochem. 2003;86:165–172. [PubMed]
  • Langston JW, Langston EB, Irwin I. MPTP-induced Parkinsonism in human and non-human primates—Clinical and experimental aspects. Acta. Neurol. Scand. Suppl. 1984;100:49–54. [PubMed]
  • Lee I, Lehner B, Crombie C, Wong W, Fraser AG, Marcotte EM. A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans. Nat. Genet. 2008;40:181–188. [PubMed]
  • Lee MH, Ahn B, Choi IS, Koo HS. The gene expression and deficiency phenotypes of Cockayne syndrome B protein in Caenorhabditis elegans. FEBS Lett. 2002;522:47–51. [PubMed]
  • Lee RC, Hammell CM, Ambros V. Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. RNA. 2006;12:589–597. [PMC free article] [PubMed]
  • Lee SJ, Yook JS, Han SM, Koo HS. A Werner syndrome protein homolog affects C. elegans development, growth rate, life span and sensitivity to DNA damage by acting at a DNA damage checkpoint. Development. 2004;131:2565–2575. [PubMed]
  • Lee SS, Lee RY, Fraser AG, Kamath RS, Ahringer J, Ruvkun G. A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat. Genet. 2003;33:40–48. [PubMed]
  • Leiers B, Kampkotter A, Grevelding CG, Link CD, Johnson TE, Henkle-Duhrsen K. A stress-responsive glutathione S-transferase confers resistance to oxidative stress in Caenorhabditis elegans. Free Radic. Biol. Med. 2003;34:1405–1415. [PubMed]
  • Lettre G, Hengartner MO. Developmental apoptosis in C. elegans: A complex CEDnario. Nat. Rev. Mol. Cell Biol. 2006;7:97–108. [PubMed]
  • Levitan D, Greenwald I. Facilitation of lin-12-mediated signalling by sel-12, a Caenorhabditis elegans S182 Alzheimer's disease gene. Nature. 1995;377:351–354. [PubMed]
  • Li S, Armstrong CM, Bertin N, Ge H, Milstein S, Boxem M, Vidalain PO, Han JD, Chesneau A, Hao T, et al. A map of the interactome network of the metazoan C. elegans. Science. 2004;303:540–543. [PMC free article] [PubMed]
  • Li X, Greenwald I. HOP-1, a Caenorhabditis elegans presenilin, appears to be functionally redundant with SEL-12 presenilin and to facilitate LIN-12 and GLP-1 signaling. Proc. Natl Acad. Sci. USA. 1997;94:12204–12209. [PMC free article] [PubMed]
  • Liao VH, Yu CW. Caenorhabditis elegans gcs-1 confers resistance to arsenic-induced oxidative stress. Biometals. 2005;18:519–528. [PubMed]
  • Link CD. Transgenic invertebrate models of age-associated neurodegenerative diseases. Mech. Ageing Dev. 2001;122:1639–1649. [PubMed]
  • Link CD, Taft A, Kapulkin V, Duke K, Kim S, Fei Q, Wood DE, Sahagan BG. Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer's disease model. Neurobiol. Aging. 2003;24:397–413. [PubMed]
  • Liou HH, Tsai MC, Chen CJ, Jeng JS, Chang YC, Chen SY, Chen RC. Environmental risk factors and Parkinson's disease: A case-control study in Taiwan. Neurology. 1997;48:1583–1588. [PubMed]
  • Luo Y. Alzheimer's disease, the nematode Caenorhabditis elegans, and ginkgo biloba leaf extract. Life Sci. 2006;78:2066–2072. [PubMed]
  • Malone EA, Thomas JH. A screen for nonconditional dauer-constitutive mutations in Caenorhabditis elegans. Genetics. 1994;136:879–886. [PMC free article] [PubMed]
  • Marroquin LD, Elyassnia D, Griffitts JS, Feitelson JS, Aroian RV. Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans. Genetics. 2000;155:1693–1699. [PMC free article] [PubMed]
  • Marvanova M, Nichols CD. Identification of neuroprotective compounds of Caenorhabditis elegans dopaminergic neurons against 6-OHDA. J. Mol. Neurosci. 2007;31:127–137. [PubMed]
  • Mattson MP. Apoptosis in neurodegenerative disorders. Nat. Rev. Mol. Cell. Biol. 2000;1:120–129. [PubMed]
  • Maupus E. Modes et formes de reproduction des nematodes. Arch. Zool. Exp. Gen. 1900;8:463–624.
  • Mee CJ, Tomlinson SR, Perestenko PV, de Pomerai D, Duce IR, Usherwood PNR, Bell DR. Latrophilin is required for toxicity of black widow spider venom in Caenorhabditis elegans. Biochem. J. 2004;378:185–191. [PMC free article] [PubMed]
  • Melstrom PC, Williams PL. Reversible AChE inhibitors in C. elegans vs. rats, mice. Biochem. Biophys. Res. Commun. 2007;357:200–205. [PubMed]
  • Menzel R, Yeo HL, Rienau S, Li S, Steinberg CE, Sturzenbaum SR. Cytochrome P450s and short-chain dehydrogenases mediate the toxicogenomic response of PCB52 in the nematode Caenorhabditis elegans. J. Mol. Biol. 2007;370:1–13. [PubMed]
  • Meyer JN, Boyd WA, Azzam GA, Haugen AC, Freedman JH, Van Houten B. Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. Genome Biol. 2007;8:R70. [PMC free article] [PubMed]
  • Miller LM, Hartman PS. The effects of benzo[a]pyrene (cough cough!) on C. elegans. Worm Breed. Gaz. 1998;15:43.
  • Mills DK, Hartman PS. Lethal consequences of simulated solar radiation on the nematode Caenorhabditis elegans in the presence and absence of photosensitizers. Photochem. Photobiol. 1998;68:816–823. [PubMed]
  • Morck C, Axang C, Pilon M. A genetic analysis of axon guidance in the C. elegans pharynx. Dev. Biol. 2003;260:158–175. [PubMed]
  • Morgan PG, Kayser EB, Sedensky MM. WormBook. 2007. C. elegans and volatile anesthetics; pp. 1–11. [PubMed]
  • Mori I, Sasakura H, Kuhara A. Worm thermotaxis: A model system for analyzing thermosensation and neural plasticity. Curr. Opin. Neurobiol. 2007;17:712–719. [PubMed]
  • Munakata N, Morohoshi F. DNA glycosylase activities in the nematode Caenorhabditis elegans. Mutat. Res. 1986;165:101–107.
  • Murakami S. Caenorhabditis elegans as a model system to study aging of learning and memory. Mol. Neurobiol. 2007;35:85–94. [PubMed]
  • Murakami S, Johnson TE. A genetic pathway conferring life extension and resistance to UV stress in Caenorhabditis elegans. Genetics. 1996;143:1207–1218. [PMC free article] [PubMed]
  • Mutwakil MH, Reader JP, Holdich DM, Smithurst PR, Candido EPM, Jones D, Stringham EG, de Pomerai DI. Use of stress-inducible transgenic nematodes as biomarkers of heavy metal pollution in water samples from an English river system. Arch. Environ. Contam. Toxicol. 1997;32:146–153. [PubMed]
  • Nass R, Hahn MK, Jessen T, McDonald PW, Carvelli L, Blakely RD. A genetic screen in Caenorhabditis elegans for dopamine neuron insensitivity to 6-hydroxydopamine identifies dopamine transporter mutants impacting transporter biosynthesis and trafficking. J. Neurochem. 2005;94:774–785. [PubMed]
  • Nass R, Hall DH, Miller DM, III, Blakely RD. Neurotoxin-induced degeneration of dopamine neurons in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA. 2002;99:3264–3269. [PMC free article] [PubMed]
  • National Research Council (NRC) Scientific Frontiers in Developmental Toxicology and Risk Assessment. Washington, DC: The National Academies Press; 2000. pp. 296–308.
  • Neher DA, Sturzenbaum SR. Extra-long PCR, an identifier of DNA adducts in single nematodes (Caenorhabditis elegans) Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol. 2006;144:279–285. [PubMed]
  • Niwa R, Zhou F, Li C, Slack FJ. The expression of the Alzheimer's amyloid precursor protein-like gene is regulated by developmental timing microRNAs and their targets in Caenorhabditis elegans. Dev. Biol. 2008;315:418–425. [PMC free article] [PubMed]
  • Nyamsuren O, Faggionato D, Loch W, Schulze E, Baumeister R. A mutation in CHN-1/CHIP suppresses muscle degeneration in Caenorhabditis elegans. Dev. Biol. 2007;312:193–202. [PubMed]
  • O'Connell KF, Leys CM, White JG. A genetic screen for temperature-sensitive cell-division mutants of Caenorhabditis elegans. Genetics. 1998;149:1303–1321. [PMC free article] [PubMed]
  • Olsen A, Vantipalli MC, Lithgow GJ. Using Caenorhabditis elegans as a model for aging and age-related diseases. Ann. N. Y. Acad. Sci. 2006;1067:120–128. [PubMed]
  • O'Neil N, Rose A. WormBook. 2005. DNA repair.
  • Ong WY, Farooqui AA. Iron, neuroinflammation, and Alzheimer's disease. J. Alzheimers Dis. 2005;8:183–215. [PubMed]
  • Park HK, Suh D, Hyun M, Koo HS, Ahn B. A DNA repair gene of Caenorhabditis elegans: A homolog of human XPF. DNA Repair. 2004;3:1375–1383. [PubMed]
  • Park HK, Yook JS, Koo HS, Choi IS, Ahn B. The Caenorhabditis elegans XPA homolog of human XPA. Mol. Cells. 2002;14:50–55. [PubMed]
  • Parker JA, Connolly JB, Wellington C, Hayden M, Dausset J, Neri C. Expanded polyglutamines in Caenorhabditis elegans cause axonal abnormalities and severe dysfunction of PLM mechanosensory neurons without cell death. Proc. Natl Acad. Sci. USA. 2001;98:13318–13323. [PMC free article] [PubMed]
  • Peredney CL, Williams PL. Utility of Caenorhabditis elegans for assessing heavy metal contamination in artificial soil. Arch. Environ. Contam. Toxicol. 2000;39:113–118. [PubMed]
  • Peterson RT, Nass R, Dong K, Boyd WA, Freedman JH, Narahashi T. Use of non-mammalian alternative models for neurotoxicological study. Neurotoxicology. 2008;29:545–554. [PMC free article] [PubMed]
  • Pinkston-Gosse J, Kenyon C. DAF-16/FOXO targets genes that regulate tumor growth in Caenorhabditis elegans. Nat. Genet. 2007;39:1403–1409. [PubMed]
  • Plasterk RHA. Reverse genetics: From gene sequence to mutant worm. Methods Cell Biol. 1995;48:59–80. [PubMed]
  • Plasterk RHA, Groenen JTM. Targeted alterations of the Caenorhabditis-elegans genome by transgene instructed DNA double strand break repair following Tc1 excision. EMBO J. 1992;11:287–290. [PMC free article] [PubMed]
  • Pothof J, van Haaften G, Thijssen K, Kamath RS, Fraser AG, Ahringer J, Plasterk RH, Tijsterman M. Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi. Genes Dev. 2003;17:443–448. [PMC free article] [PubMed]
  • Poulin G, Nandakumar R, Ahringer J. Genome-wide RNAi screens in Caenorhabditis elegans: Impact on cancer research. Oncogene. 2004;23:8340–8345. [PubMed]
  • Poysky J. Behavior patterns in Duchenne muscular dystrophy: Report on the Parent Project Muscular Dystrophy Behavior Workshop 8–9 of December 2006, Philadelphia, USA. Neuromuscul. Disord. 2007;17:986–994. [PubMed]
  • Pulak R. Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system. Methods Mol. Biol. 2006;351:275–286. [PubMed]
  • Quinn CC, Wadsworth WG. Axon guidance: Ephrins at WRK on the midline. Curr. Biol. 2006;16:R954–R955. [PubMed]
  • Rajini PS, Melstrom PC, Williams PL. A comparative study on the relationship between various toxicological endpoints in Caenorhabditis elegans exposed to organophosphorus insecticides. Toxicol. Environ. Health. 2008;71:1043–1050. [PubMed]
  • Rand JB. WormBook. 2007. Acetylcholine; pp. 1–21. [PubMed]
  • Rand JB, Nonet ML. Synaptic Transmission. New York: Cold Spring Harbor Laboratory Press; 1997.
  • Rankin CH, Beck CD, Chiba CM. Caenorhabditis elegans: A new model system for the study of learning and memory. Behav. Brain Res. 1990;37:89–92. [PubMed]
  • Ray WJ, Yao M, Nowotny P, Mumm J, Zhang W, Wu JY, Kopan R, Goate AM. Evidence for a physical interaction between presenilin and Notch. Proc. Natl Acad. Sci. USA. 1999;96:3263–3268. [PMC free article] [PubMed]
  • Rea SL, Ventura N, Johnson TE. Relationship between mitochondrial electron transport chain dysfunction, development, and life extension in Caenorhabditis elegans. PLoS Biol. 2007;5:e259. [PMC free article] [PubMed]
  • Reichert K, Menzel R. Expression profiling of five different xenobiotics using a Caenorhabditis elegans whole genome microarray. Chemosphere. 2005;61:229–237. [PubMed]
  • Richmond J. WormBook. 2005. Synaptic function; pp. 1–14. [PubMed]
  • Riddle DL, Blumenthal T, Meyer BJ, Preiss JR. C. elegans II. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1997.
  • Ritz B, Yu F. Parkinson's disease mortality and pesticide exposure in California 1984–1994. Int. J. Epidemiol. 2000;29:323–329. [PubMed]
  • Roh JY, Lee J, Choi J. Assessment of stress-related gene expression in the heavy metal-exposed nematode Caenorhabditis elegans: A potential biomarker for metal-induced toxicity monitoring and environmental risk assessment. Environ. Toxicol. Chem. 2006;25:2946–2956. [PubMed]
  • Rohde CB, Zeng F, Gonzalez-Rubio R, Angel M, Yanik MF. Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proc. Natl Acad. Sci. USA. 2007;104:13891–13895. [PMC free article] [PubMed]
  • Rosenbluth RE, Cuddeford C, Baillie DL. Mutagenesis in Caenorhabditis elegans. II. A spectrum of mutational events induced with 1500 R of gamma-radiation. Genetics. 1985;109:493–511. [PMC free article] [PubMed]
  • Rosenbluth RE, Cuddeford C, Baillie DL. Mutagenesis in Caenorhabditis elegans I: A rapid eukaryotic mutagen test system using the reciprocal translocation eT1(III;V) Mutat. Res. 1983;110:39–48.
  • Rushforth AM, Saari B, Anderson P. Site-selected insertion of the transposon Tc1 into a Caenorhabditis-elegans myosin light chain gene. Mol. Cell Biol. 1993;13:902–910. [PMC free article] [PubMed]
  • Sadaie T, Sadaie Y. Rad-2-dependent repair of radiation-induced chromosomal-aberrations in Caenorhabditis-elegans. Mutat. Res. 1989;218:25–31. [PubMed]
  • Saffih-Hdadi K, Bruckler L, Amichot M, Belzunces L. Modeling impact of parathion and its metabolite paraoxon on the nematode Caenorhabditis elegans in soil. Environ. Toxicol. Chem. 2005;24:1387–1394. [PubMed]
  • Sakaguchi-Nakashima A, Meir JY, Jin Y, Matsumoto K, Hisamoto N. LRK-1, a C. elegans PARK8-related kinase, regulates axonal-dendritic polarity of SV proteins. Curr. Biol. 2007;17:592–598. [PubMed]
  • Salinas LS, Maldonado E, Navarro RE. Stress-induced germ cell apoptosis by a p53 independent pathway in Caenorhabditis elegans. Cell Death Differ. 2006;13:2129–2139. [PubMed]
  • Sambongi Y, Nagae T, Liu Y, Yoshimizu T, Takeda K, Wada Y, Futai M. Sensing of cadmium and copper ions by externally exposed ADL, ASE, and ASH neurons elicits avoidance response in Caenorhabditis elegans. Neuroreport. 1999;10:753–757. [PubMed]
  • Samii A, Nutt JG, Ransom BR. Parkinson's disease. Lancet. 2004;363:1783–1793. [PubMed]
  • Sanyal S, Wintle RF, Kindt KS, Nuttley WM, Arvan R, Fitzmaurice P, Bigras E, Merz DC, Hebert TE, van der Kooy D, et al. Dopamine modulates the plasticity of mechanosensory responses in Caenorhabditis elegans. EMBO J. 2004;23:473–482. [PMC free article] [PubMed]
  • Savory J, Herman MM, Ghribi O. Intracellular mechanisms underlying aluminum-induced apoptosis in rabbit brain. J. Inorg. Biochem. 2003;97:151–154. [PubMed]
  • Sawin ER, Ranganathan R, Horvitz HR. C. elegans locomotory rate is modulated by the environment through a dopaminergic pathway and by experience through a serotonergic pathway. Neuron. 2000;26:619–631. [PubMed]
  • Schafer WR. WormBook. 2006. Neurophysiological methods in C. elegans: An introduction; pp. 1–4. [PubMed]
  • Schmidt E, Seifert M, Baumeister R. Caenorhabditis elegans as a model system for Parkinson's disease. Neurodegener. Dis. 2007;4:199–217. [PubMed]
  • Schmitz C, Kinge P, Hutter H. Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive Caenorhabditis elegans strain nre-1(hd20) lin-15b(hd126) Proc. Natl Acad. Sci. USA. 2007;104:834–839. [PMC free article] [PubMed]
  • Schulenburg H, Muller S. Natural variation in the response of Caenorhabditis elegans towards Bacillus thuringiensis. Parasitology. 2004;128:433–443. [PubMed]
  • Schumacher B, Schertel C, Wittenburg N, Tuck S, Mitani S, Gartner A, Conradt B, Shaham S. C-elegans ced-13 can promote apoptosis and is induced in response to DNA damage. Cell Death Differ. 2005;12:153–161. [PubMed]
  • Semchuk KM, Love EJ, Lee RG. Parkinson's disease and exposure to agricultural work and pesticide chemicals. Neurology. 1992;42:1328–1335. [PubMed]
  • Shatilla A, Ishchenko AA, Saparbaev M, Ramotar D. Characterization of Caenorhabditis elegans exonuclease-3 and evidence that a Mg2+-dependent variant exhibits a distinct mode of action on damaged DNA. Biochemistry. 2005a;44:12835–12848. [PubMed]
  • Shatilla A, Leduc A, Yang XM, Ramotar D. Identification of two apurinic/apyrimidinic endonucleases from Caenorhabditis elegans by cross-species complementation. DNA Repair. 2005b;4:655–670. [PubMed]
  • Shatilla A, Ramotar D. Embryonic extracts derived from the nematode Caenorhabditis elegans remove uracil from DNA by the sequential action of uracil-DNA glycosylase and AP (apurinic/apyrimidinic) endonuclease. Biochem. J. 2002;365:547–553. [PMC free article] [PubMed]
  • Sherwood DR, Butler JA, Kramer JM, Sternberg PW. FOS-1 promotes basement-membrane removal during anchor-cell invasion in C. elegans. Cell. 2005;121:951–962. [PubMed]
  • Silhankova M, Korswagen HC. Migration of neuronal cells along the anterior-posterior body axis of C. elegans: Wnts are in control. Curr. Opin. Genet. Dev. 2007;17:320–325. [PubMed]
  • Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH. Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. PLoS Biol. 2003;1:E12. [PMC free article] [PubMed]
  • Simmer F, Tijsterman M, Parrish S, Koushika SP, Nonet ML, Fire A, Ahringer J, Plasterk RH. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr. Biol. 2002;12:1317–1319. [PubMed]
  • Simonetta SH, Golombek DA. An automated tracking system for Caenorhabditis elegans locomotor behavior and circadian studies application. J. Neurosci. Methods. 2007;161:273–280. [PubMed]
  • Smialowska A, Baumeister R. Presenilin function in Caenorhabditis elegans. Neurodegener. Dis. 2006;3:227–232. [PubMed]
  • Sochova I, Hofman J, Holoubek I. Effects of seven organic pollutants on soil nematode Caenorhabditis elegans. Environ. Int. 2007;33:798–804. [PubMed]
  • Springer W, Hoppe T, Schmidt E, Baumeister R. A Caenorhabditis elegans Parkin mutant with altered solubility couples alpha-synuclein aggregation to proteotoxic stress. Hum. Mol. Genet. 2005;14:3407–3423. [PubMed]
  • Stergiou L, Doukoumetzidis K, Sendoel A, Hengartner MO. The nucleotide excision repair pathway is required for UV-C-induced apoptosis in Caenorhabditis elegans. Cell Death Differ. 2007;14:1129–1138. [PubMed]
  • Stergiou L, Hengartner MO. Death and more: DNA damage response pathways in the nematode C. elegans. Cell Death Differ. 2004;11:21–28. [PubMed]
  • Stewart HI, Rosenbluth RE, Baillie DL. Most ultraviolet-irradiation induced mutations in the nematode Caenorhabditis-elegans are chromosomal rearrangements. Mutat. Res. 1991;249:37–54. [PubMed]
  • St-Laurent JF, Gagnon SN, Dequen F, Hardy I, Desnoyers S. Altered DNA damage response in Caenorhabditis elegans with impaired poly(ADP-ribose) glycohydrolases genes expression. DNA Repair. 2007;6:329–343. [PubMed]
  • Stringham-Durovic EG, Candido EPM. Transgenic hsp16-lacZ strains of the soil nematode Caenorhabditis elegans as biological monitors of environmental stress. Environmental Toxicology and Chemistry. 1994;13:1211–1220.
  • Sulston JE. Neuronal cell lineages in the nematode Caenorhabditis elegans. Cold Spring Harb. Symp. Quant. Biol. 1983;48(Pt 2):443–452. [PubMed]
  • Sulston JE. Caenorhabditis elegans: The cell lineage and beyond (Nobel lecture) Chembiochem. 2003;4:688–696. [PubMed]
  • Sulston JE, Schierenberg E, White JG, Thomson JN. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 1983;100:64–119. [PubMed]
  • Tabara H, Yigit E, Siomi H, Mello CC. The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans. Cell. 2002;109:861–871. [PubMed]
  • Takanami T, Sato S, Ishihara T, Katsura I, Takahashi H, Higashitani A. Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination. DNA Res. 1998;5:373–377. [PubMed]
  • Tatara CP, Newman MC, McCloskey JT, Williams PL. Use of ion characteristics to predict relative toxicity of mono-, di- and trivalent metal ions: Caenorhabditis elegans LC50. Aquat. Toxicol. 1998;42:255–269.
  • Thiruchelvam M, Brockel BJ, Richfield EK, Baggs RB, Cory-Slechta DA. Potentiated and preferential effects of combined paraquat and maneb on nigrostriatal dopamine systems: Environmental risk factors for Parkinson's disease? Brain Res. 2000a;873:225–234. [PubMed]
  • Thiruchelvam M, Richfield EK, Baggs RB, Tank AW, Cory-Slechta DA. The nigrostriatal dopaminergic system as a preferential target of repeated exposures to combined paraquat and maneb: Implications for Parkinson's disease. J. Neurosci. 2000b;20:9207–9214. [PubMed]
  • Tijsterman M, Pothof J, Plasterk RHA. Frequent germline mutations and somatic repeat instability in DNA mismatch-repair-deficient Caenorhabditis elegans. Genetics. 2002;161:651–660. [PMC free article] [PubMed]
  • Tops BB, Tabara H, Sijen T, Simmer F, Mello CC, Plasterk RH, Ketting RF. RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. Nucleic Acids Res. 2005;33:347–355. [PMC free article] [PubMed]
  • Traunspurger W, Haitzer M, Hoss S, Beier S, Ahlf W, Steinberg C. Ecotoxicological assessment of aquatic sediments with Caenorhabditis elegans (nematoda) A method for testing liquid medium and whole-sediment samples. Environ. Toxicol. Chem. 1997;16:245–250.
  • Truglio JJ, Croteau DL, Van Houten B, Kisker C. Prokaryotic nucleotide excision repair: The UvrABC system. Chem. Rev. 2006;106:233–252. [PubMed]
  • Tseng YY, Yu CW, Liao VHC. Caenorhabditis elegans expresses a functional ArsA. FEBS J. 2007;274:2566–2572. [PubMed]
  • Tsibidis GD, Tavernarakis N. Nemo: A computational tool for analyzing nematode locomotion. BMC Neurosci. 2007;8:86. [PMC free article] [PubMed]
  • Tullet JM, Hertweck M, An JH, Baker J, Hwang JY, Liu S, Oliveira RP, Baumeister R, Blackwell TK. Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans. Cell. 2008;132:1025–1038. [PMC free article] [PubMed]
  • Tvermoes B, Freedman JH. SOT Meeting March 16-20, 2008. 2008. Caenorhabditis elegans gene, Numr-1, assembles into nuclear stress granules after cadmium treatment. Society of Toxicology, Seattle, WA. p. 331–332.
  • Uversky VN. Neurotoxicant-induced animal models of Parkinson's disease: Understanding the role of rotenone, maneb and paraquat in neurodegeneration. Cell Tissue Res. 2004;318:225–241. [PubMed]
  • Vanfleteren JR. Oxidative stress and ageing in Caenorhabditis elegans. Biochem. J. 1993;292(Pt 2):605–608. [PMC free article] [PubMed]
  • van Haaften G, Plasterk RHA, Tijsterman M. Genomic instability and cancer: Scanning the Caenorhabditis elegans genome for tumor suppressors. Oncogene. 2004a;23:8366–8375. [PubMed]
  • van Haaften G, Vastenhouw NL, Nollen EAA, Plasterk RHA, Tijsterman M. Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality. Proc. Natl Acad. Sci. USA. 2004b;101:12992–12996. [PMC free article] [PubMed]
  • van Ham TJ, Thijssen KL, Breitling R, Hofstra RM, Plasterk RH, Nollen EA. C. elegans model identifies genetic modifiers of alpha-synuclein inclusion formation during aging. PLoS Genet. 2008;4:e1000027. [PMC free article] [PubMed]
  • Vartiainen S, Pehkonen P, Lakso M, Nass R, Wong G. Identification of gene expression changes in transgenic C. elegans overexpressing human alpha-synuclein. Neurobiol. Dis. 2006;22:477–486. [PubMed]
  • Ved R, Saha S, Westlund B, Perier C, Burnam L, Sluder A, Hoener M, Rodrigues CM, Alfonso A, Steer C, et al. Similar patterns of mitochondrial vulnerability and rescue induced by genetic modification of alpha-synuclein, parkin, and DJ-1 in Caenorhabditis elegans. J. Biol. Chem. 2005;280:42655–42668. [PMC free article] [PubMed]
  • Ventura N, Rea SL, Testi R. Long-lived C. elegans mitochondrial mutants as a model for human mitochondrial-associated diseases. Exp. Gerontol. 2006;41:974–991. [PubMed]
  • Wadsworth WG. Moving around in a worm: Netrin UNC-6 and circumferential axon guidance in C. elegans. Trends Neurosci. 2002;25:423–429. [PubMed]
  • Wang H, Lim PJ, Yin C, Rieckher M, Vogel BE, Monteiro MJ. Suppression of polyglutamine-induced toxicity in cell and animal models of Huntington's disease by ubiquilin. Hum. Mol. Genet. 2006;15:1025–1041. [PubMed]
  • Wang S, Tang M, Pei B, Xiao X, Wang J, Hang H, Wu L. Cadmium-induced germline apoptosis in Caenorhabditis elegans: The roles of HUS1, p53, and MAPK signaling pathways. Toxicol. Sci. 2008;102:345–351. [PubMed]
  • Wang XW, Liou YC, Ho B, Ding JL. An evolutionarily conserved 16-kDa thioredoxin-related protein is an antioxidant which regulates the NF-kappaB signaling pathway. Free Radic. Biol. Med. 2007a;42:247–259. [PubMed]
  • Wang YM, Pu P, Le WD. ATP depletion is the major cause of MPP+ induced dopamine neuronal death and worm lethality in alpha-synuclein transgenic C. elegans. Neurosci. Bull. 2007b;23:329–335. [PubMed]
  • Watanabe M, Mitani N, Ishii N, Miki K. A mutation in a cuticle collagen causes hypersensitivity to the endocrine disrupting chemical, bisphenol A, in Caenorhabditis elegans. Mutat. Res. 2005;570:71–80. [PubMed]
  • Wei JZ, Hale K, Carta L, Platzer E, Wong C, Fang SC, Aroian RV. Bacillus thuringiensis crystal proteins that target nematodes. Proc. Natl Acad. Sci. USA. 2003;100:2760–2765. [PMC free article] [PubMed]
  • Weidhaas JB, Eisenmann DM, Holub JM, Nallur SV. A Caenorhabditis elegans tissue model of radiation-induced reproductive cell death. Proc. Natl Acad. Sci. USA. 2006;103:9946–9951. [PMC free article] [PubMed]
  • White JG, Southgate E, Thomson JN, Brenner S. The structure of the nervous system of the nematode Caenorhabditis elegans. Philos. Trans. R. Soc. Lond. B Biol. Sci. 1986;314:1–340. [PubMed]
  • Williams PL, Anderson GL, Johnstone JL, Nunn AD, Tweedle MF, Wedeking P. Caenorhabditis elegans as an alternative animal species. J. Toxicol. Environ. Health A. 2000;61:641–647. [PubMed]
  • Williams PL, Dusenbery DB. Using the nematode Caenorhabditis elegans to predict mammalian acute lethality to metallic salts. Toxicol. Ind. Health. 1988;4:469–478. [PubMed]
  • Williams PL, Dusenbery DB. Aquatic toxicity testing using the nematode, Caenorhabditis elegans. Environ. Toxicol. Chem. 1990a;9:1285–1290.
  • Williams PL, Dusenbery DB. A promising indicator of neurobehavioral toxicity using the nematode Caenorhabditis elegans and computer tracking. Toxicol. Ind. Health. 1990b;6:425–440. [PubMed]
  • Wilson JM, Levey AI, Rajput A, Ang L, Guttman M, Shannak K, Niznik HB, Hornykiewicz O, Pifl C, Kish SJ. Differential changes in neurochemical markers of striatal dopamine nerve terminals in idiopathic Parkinson's disease. Neurology. 1996;47:718–726. [PubMed]
  • Wolozin B, Saha S, Guillily M, Ferree A, Riley M. Investigating convergent actions of genes linked to familial Parkinson's disease. Neurodegener. Dis. 2008;5:182–185. [PMC free article] [PubMed]
  • Wood-Kaczmar A, Gandhi S, Wood NW. Understanding the molecular causes of Parkinson's disease. Trends Mol. Med. 2006;12:521–528. [PubMed]
  • Wu Y, Luo Y. Transgenic C. elegans as a model in Alzheimer's research. Curr. Alzheimer Res. 2005;2:37–45. [PubMed]
  • Wu Y, Wu Z, Butko P, Christen Y, Lambert MP, Klein WL, Link CD, Luo Y. Amyloid-beta-induced pathological behaviors are suppressed by Ginkgo biloba extract EGb 761 and ginkgolides in transgenic Caenorhabditis elegans. J. Neurosci. 2006;26:13102–13113. [PubMed]
  • Wu Z, Ghosh-Roy A, Yanik MF, Zhang JZ, Jin Y, Chisholm AD. Caenorhabditis elegans neuronal regeneration is influenced by life stage, ephrin signaling, and synaptic branching. Proc. Natl Acad. Sci. USA. 2007;104:15132–15137. [PMC free article] [PubMed]
  • Yamamoto K, Honda S, Ishii N. Properties of an oxygen-sensitive mutant mev-3 of the nematode Caenorhabditis elegans. Mutat. Res. 1996;358:1–6. [PubMed]
  • Yanase S, Yasuda K, Ishii N. Adaptive responses to oxidative damage in three mutants of Caenorhabditis elegans (age-1, mev-1 and daf-16) that affect life span. Mech. Ageing Dev. 2002;123:1579–1587. [PubMed]
  • Yang W, Li J, Hekimi S. A measurable increase in oxidative damage due to reduction in superoxide detoxification fails to shorten the life span of long-lived mitochondrial mutants of Caenorhabditis elegans. Genetics. 2007;177:2063–2074. [PMC free article] [PubMed]
  • Ye H, Ye B, Wang D. Trace administration of vitamin E can retrieve and prevent UV-irradiation and metal exposure-induced memory deficits in nematode Caenorhabditis elegans. Neurobiol. Learn. Mem. (in press) [PubMed]
  • Zhang SL, Yeromin AV, Zhang XH, Yu Y, Safrina O, Penna A, Roos J, Stauderman KA, Cahalan MD. Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. Proc. Natl Acad. Sci. USA. 2006;103:9357–9362. [PMC free article] [PubMed]
  • Zhong W, Sternberg PW. Genome-wide prediction of C. elegans genetic interactions. Science. 2006;311:1481–1484. [PubMed]

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