Logo of jexbotLink to Publisher's site
J Exp Bot. 2008 Oct; 59(13): 3621–3634.
PMCID: PMC2561157

Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins


The ligandin activity of specific glutathione S-transferases (GSTs) is necessary for the transport of anthocyanins from the cytosol to the plant vacuole. Five GSTs were purified from Vitis vinifera L. cv. Gamay Fréaux cell suspension cultures by glutathione affinity chromatography. These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. The corresponding coding sequences were cloned, with heterologous expression in Escherichia coli used to confirm GST activity. Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (PAL, CHS, DFR, and UFGT) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, VvGST1 and VvGST4, respectively. The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. Furthermore, the differential induction of VvGST1 and VvGST4 in suspension cells and grape berries suggests functional differences between these two proteins. Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation in planta and in vitro suspension cells.

Keywords: Anthocyanin transport, glutathione S-transferase, ligandin, plant cell culture, Vitis vinifera


Plants contain a variety of glutathione-binding proteins, including glutathione lyases, peroxidases, reductases, and S-transferases (GSTs) (Marrs, 1996). The GSTs are unique in this group as they are all soluble or loosely membrane-associated dimers with a monomeric size of 15–28 kDa, and together comprise 1–3.5% of total cellular protein (Droog et al., 1995; Pairoba and Walbot, 2003). GSTs catalyse the conjugation of the tripeptide glutathione (γ-Glu-Cys-Gly; GSH) to a variety of electrophilic compounds to direct them to specific sites both intra- and extracellularly. In mammals, GST subunits are classified into α, μ, π, σ, and θ classes on the basis of amino acid identity, immuno-cross-reactivity, and substrate specificity (Buetler and Eaton, 1992; Mannervik et al., 1992). The majority of plant GSTs are most similar to the θ class, and are further classified into three phylogenetic types based on sequence similarity and intron/exon structure (Droog et al., 1993; Droog, 1997). Type I GSTs, with two introns, have been identified in numerous plant species. Type II GSTs have been identified in wheat and carnation, with the gene in carnation containing nine introns (McGonigle et al., 2000). Type III GSTs contain a single intron and make up the second largest family of plant GSTs, including many of the GSTs originally identified as auxin-regulated proteins (Takahashi et al., 1995; Edwards and Dixon, 2005).

GSTs, originally characterized by their ability to modify xenobiotics covalently by glutathionation, are rapidly induced by their substrates—in particular plant herbicides (Marrs et al., 1995; Aharoni et al., 2002; Dean et al., 2005). In addition, GSTs act as non-enzymatic carrier proteins (ligandins) enabling intracellular shuttling of endogenous compounds in animals, plants, and prokaryotes. Within plants, these ligands include indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), labile cytochrome precursors, and the ubiquitous plant pigments, anthocyanins (Jones, 1994; Bilang and Sturm, 1995; Marrs et al., 1995; Mueller et al., 2000; Lederer and Böger, 2005). The soybean GH2/4 gene is inducible not only by strong auxins (2,4-D; 2,4,5-T; α-NAA) and salicylic acid (SA), but also by numerous other electrophilic compounds, including weak auxins (β-NAA), inactive auxin analogues (2,3-D and 2,3,6-T), and inactive SA analogues (3-hydroxybenzoic acid, 4-hydroxybenzoic acid) (Ulmasov et al., 1994, 1995). Furthermore, the Hmgst-1 protein from Hyoscyamus muticus able to bind IAA exhibits an expression profile that correlates with accumulation of IAA, suggesting that this GST is involved in intracellular IAA transport (Bilang et al., 1993; Bilang and Sturm, 1995). Thus, it is clear that different auxins and potentially other ligands induce and bind GSTs differently. Beyond this work on auxins, few studies relate GST transcription or expression with accumulation of endogenous substrates. The well-characterized anthocyanin biosynthetic pathway in grapevines thus provides an ideal target for analysing transcriptional correlation of GSTs with another family of non-substrate ligands.

The state of protonation influences the colour of anthocyanins, and in many plant species they are concentrated within structures known as anthocyanic vacuolar inclusions, within the acidic central vacuole (Pecket and Small, 1980; Conn et al., 2003). Their synthesis, however, has been demonstrated to occur predominantly in the cytosol. Indeed for Arabidopsis, the anthocyanin biosynthetic complex has been localized to the cytosolic face of the rough endoplasmic reticulum (reviewed in Winkel, 2004). Furthermore, the localization of an anthocyanin methyltransferase from Petunia hybrida (Jonsson et al., 1983) and anthocyanin acyltransferases from Gentiana triflora (Fujiwara et al., 1998) and Perilla frutescens (Yonekura-Sakakibara et al., 2000) to the cytosol suggests that complete anthocyanins, rather than intermediates, are transported into the vacuole.

Gene knockout and complementation studies have demonstrated that GSTs are indelibly involved in anthocyanin transport. A Zea mays knockout mutant of a single GST [Bronze-2 (Bz2)] accumulated anthocyanins in the cytosol only, at concentrations <10% of those found in wild-type lines (Mueller and Walbot, 2001; Goodman et al., 2004). Anthocyanin-transporting GSTs have been identified by large-scale mutagenesis screens in other species, including petunia (An9; Alfenito et al., 1998), carnation (Fl3; Larsen et al., 2003), and Arabidopsis (TT19; Kitamura et al., 2004). Kitamura et al. (2004) demonstrated that TT19 played an additional role in seed deposition of proanthocyanidins (tannins). While binding of both Bz2 and An9 to anthocyanins has not been directly shown, various studies have inferred this interaction by the use of equilibrium dialysis and tryptophan quenching with the recombinant proteins (Marrs et al., 1995; Mueller et al., 2000). Bz2 knockout (bz2) suspension cells, when complemented with Bz2 and exposed to [35S]GSH, produced 35S-labelled compounds that co-migrated with anthocyanins when separated by thin-layer chromatography. While this is suggestive of covalent glutathionation in vivo, no further analysis was performed on these compounds (Marrs et al., 1995). Further evidence for ligandin activity in anthocyanin transport is the ability of the petunia An9 protein to complement the Bz2 mutation, despite the inability of An9 to produce detectable [35S]anthocyanin conjugates. Together, these results suggest that only ligandin activity is necessary for anthocyanin transport in these systems (Alfenito et al., 1998; Mueller et al., 2000). In addition to anthocyanins, GSTs have been shown to bind other flavonoids in vitro and be important for their transport (Mueller et al., 2000; Winefield et al., 2006).

A proteomics approach, combining affinity chromatography and mass spectrometry (MS)/Edman sequencing was used to clone GSTs from pigmented Vitis vinifera cell cultures as putative anthocyanin transport proteins. Characterization of these proteins in V. vinifera will enable a better understanding of how transport influences accumulation of anthocyanins in the vacuole. This may assist in the augmentation of anthocyanin accumulation in plant cell cultures, as previous methods including precursor feeding, end-product removal, various biotic and abiotic treatments, and overexpression of transcription factors and enzymes in the anthocyanin biosynthetic pathway have been largely unsuccessful (Grotewold et al., 1998; Zhang et al., 2002a; Grotewald, 2006). This may also facilitate more widespread manipulation of the plant metabolome, enhancing accumulation of desirable compounds including glutathione-bound aromatic compounds (Winefield et al., 2006) and other flavonoids.

Materials and methods

Plant cell culture and anthocyanin induction

Vitis vinifera cv. Gamay Fréaux var. Teinturier suspension cell line FU-01 was maintained under conditions previously described (Zhang et al., 2002b). The FU-01 cell line, selected for anthocyanin production in the dark, was originally developed and characterized by Cormier et al. (1994).

After 4 d of incubation, cultures were induced by constant irradiation with cool white light (96.8 ± 2.2 μmol s−1 m−2), 10 μM jasmonic acid, and 20 g l−1 sucrose. Control cultures received equal volumes of vehicle solutions in place of jasmonic acid and sucrose.

Growth and anthocyanin content analysis

Growth of cultures was measured by determining dry cell weight (DCW). Briefly, fresh cells were vacuum-filtered, washed with milliQ water, and transferred to pre-weighed aluminium foil squares. Fresh cell weight (FCW) was recorded and the foil parcels were dried at 90 °C for at least 48 h prior to DCW measurement.

Anthocyanins were extracted quantitatively from fresh, filter-dried suspension cells with 20 vols of acetic acid:H2O (1:1, v/v) for 1 h at room temperature. Extracts were clarified by centrifugation at 12 000 g for 5 min and the supernatant diluted 1:4 (v/v) in 40 mM Na2HPO4:80 mM citrate buffer, pH 3.0. The absorbance at 535 nm was measured on a Shimadzu UV mini 1240 UV-Vis spectrophotometer, and anthocyanins were quantified in terms of colour value (CV), as described previously (Zhang et al., 2002b). Following determination of the FCW to DCW ratio, CV/g-DCW was calculated.

Analysis of total soluble solids

Vitis vinifera cv. Shiraz berries from the Coombe Vineyard, Waite campus, Adelaide University, were analysed by a hand refractometer (Rochert, Buffalo, NY, USA) by crushing individual berries onto the optical path. For each condition, six berries were quantified (°Brix), and the results are presented as the mean ±SD.

Glutathione affinity chromatography

Glutathione affinity chromatography was performed on the 60% ammonium sulphate precipitate of whole-cell protein extracted from day 5 cultures with GS-Trap columns (GE Healthcare) according to the manufacturer's instructions. Specifically, the flow rate was set to 0.3 ml min−1 and recirculated overnight at 4 °C using a P-1 peristaltic pump (GE Healthcare). Washes and elution (10 mM glutathione) were performed at 1 ml min−1 using the ÄKTAPrime™ Plus LC system (GE Healthcare) and monitored with the online UV/conductivity recorder at 280 nm.

GST assay

The method of Habig et al. (1974) was used to assay for GST activity on protein extracts, with the following modifications. The spectrophotometric assay was performed over 2 min in 1 ml quartz cuvettes with a final concentration of 3 mM GSH and the model substrate 1 mM 1-chloro-2,4-dinitrobenzene (CDNB; ε = 9.2 mM−1 cm−1) in 100 mM phosphate buffer, pH 7.4.

Two-dimensional polyacrylamide gel electrophoresis

Protein samples were purified with the 2D-Plus One Cleanup Kit (GE Healthcare) and the pellet solubilized in 7 M urea, 2 M thiourea, 0.5% IPG buffer, pI 3–10 (GE Healthcare), 4% CHAPS, and 0.4% dithiothreitol (DTT). Thirteen centimeter IPG strips (pI3-10NL, GE Healthcare) were rehydrated in the above protein solution and focused for 48 kVh. Proteins were separated on a 10% polyacrylamide gel and subsequently stained with SYPRO Ruby (GE Healthcare) or by silver staining (Sambrook and Maniatis, 1989). The BioRad precision plus dual colour protein marker (BioRad) was run as the molecular weight standard.

Protein identification

MS fingerprinting:

Protein spots were excised from SYPRO Ruby-stained gels with a sterile scalpel and placed into separate sterile Eppendorf tubes. Samples were sent to the Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, Australia, for in-gel trypsin digestion and nano-LC MS/MS analysis as described by Zhao et al. (2004).

Edman sequencing:

Two-dimensional gel electrophoresis was performed as previously mentioned, with the gels pre-run in 1 mM thioglycolic acid. The gel was subsequently equilibrated in 10 mM CAPS buffer and transferred by semi-dry apparatus to nitrocellulose membranes. Membranes were Coomassie stained, dried, and spots excised for Edman sequencing. Automated Edman degradation was performed using a Beckman sequencer (model LF 3400) with online analysis on a Beckman system Gold HPLC (Beckman Coultier).

Sequence analysis

DNA sequencing was performed by the dideoxynucleotide chain termination reaction (BigDye Terminator version 3.1). Once verified, coding sequences were translated using the ExPaSy interface (http://au.expasy.org/tools/dna.html) and protein descriptives obtained with the PeptideMass tool (http://au.expasy.org/tools/peptide-mass.html) using default settings, enabling prediction of the molecular weight and pI of unmodified proteins (Wilkins et al., 1997; Gasteiger et al., 2005). Pairwise alignments were performed with BestFit alignment using default settings via the BioManager on ANGIS (http://www.angis.org.au). Multiple sequence alignments were produced with a Web-based version of ClustalW (http://align.genome.jp, Thompson et al., 1994) using default settings (Matrix = BLOSUM; GAPOPEN = 10, GAPEXT = 0.05). The phylogenetic tree was calculated using the Neighbor-Joining method with PHYLIP via the same website and visualized with Treeview version 1.6.6 (http://taxonomy.zoology.gla.ac.uk/rod/rod.html).

Nucleic acid extraction and reverse transcription

Genomic DNA was extracted from 0.5 g-FCW of FU-01 suspension cells, harvested after 7 d of culture with a Nucleospin® Plant II DNA extraction kit (Macherey-Nagel). Total RNA was extracted from frozen, ground cell material using Concert RNA Reagent (Invitrogen, Carslbad, CA, USA) according to the manufacturer's instructions, and the resultant pellet was resuspended in 30 ml of diethylpyrocarbonate (DEPC)-treated water. RNA samples were quantified using a Genequant Pro nucleic acid spectrophotometer (GE Healthcare). Samples were DNase treated with RQ1 RNase-free DNase I (1 U; Promega, Madison, WI, USA) in 50 ml total volume with RQ1 buffer. RNA was then re-extracted using Trizol reagent (Gibco-BRL, Carlsbad, CA, USA) and resuspended in 10 ml of DEPC-treated water. Sample quality was verified by agarose gel electrophoresis. Reverse transcription was carried out with 500 ng of DNase-treated RNA using 100 U of Superscript III reverse transcriptase (Invitrogen) and oligo(dT)20 as per the manufacturer's instructions. DTT was omitted from the buffer due to its impact on SYBR-green 1-based quantitative real-time PCR (qPCR) (Deprez et al., 2002).

Amplification of full-length GST genes

Primers were designed to amplify the open reading frame (ORF) of V. vinifera GST genes and incorporate restriction sites with Platinum Pfx DNA polymerase (Supplementary Table S1 available at JXB online) (Applied Biosystems) from both cDNA and genomic DNA. These were A-tailed (Sambrook and Maniatis, 1989) and ligated into pGEM-T Easy (Promega), with three clones sequenced to check for correct amplification. These were directionally cloned into pQE9 (N-terminal HIS fusion vector, Qiagen) and pJD288 (35S expression vector, Luehrsen et al., 1992).

VvGST3 was cloned by differential display using the Delta Differential Display Kit (Clontech) on Botrytis cinerea-infected and mock-infected grapevine plantlet leaves as per Bézier et al. (2002). A 380 bp fragment was identified possessing a plant GST gene-like C-terminus following BLAST database searches. Based on this 3′ sequence, two gene-specific primers, GST3R112 (5′-ACCATGCCTTCACGTGTTTC-3′) and GST3R174 (5′-CAAGAGGGGCCATCTAGAGC-3′), were used in two successive RACE (rapid amplification of cDNA ends)-PCR amplifications, as described by the manufacturer (SMART-RACE cDNA Amplification Kit, Clontech), to amplify the 5’ region of this transcript. The resulting 800 bp fragment was cloned in pGEM-T II vector (Promega) and three clones were sequenced.

Maize kernel bombardment

Kernels were bombarded according to the method of Alfenito et al. (1998).

Expression analysis by qPCR

Primers for phenylalanine ammonia-lyase (VvPAL, X75967), chalcone synthase (VvCHS, X575969), and UDP-glucose:flavonoid glucosyltransferase (VvUFGT, X75968) were each designed against full-length V. vinifera cDNA clones (Sparvoli et al., 1994). Primers are shown in Supplementary Table S2 at JXB online. Dihydroflavonol 4-reductase (VvDFR, X75964) and β-tubulin 1 (VvTUB, TC4564) primer sequences were provided by personal communication from Dr John Harvey (CSIRO Plant Industry, Adelaide), while primers for the grape berry housekeeping gene VvUbiquitin1 (BN000705) were previously described by Bogs et al. (2006). Samples were performed in duplicate. Amplification, detection, and relative quantification of gene expression using the ΔΔ-Ct equation developed by Pfaffl (2001) was performed using SYBR-green as described previously (Conn et al., 2008).


Purification of GSTs from grape cell cultures

GST activity of anthocyanin-accumulating V. vinifera L. Gamay Freaux suspension cells fluctuated over time, reflecting the combined activities of individual GSTs during the culture period (Fig. 1a). Both GST activity and anthocyanin accumulation were enhanced by treatment with sucrose, jasmonic acid, and light (Figs. 1a and and5a,5a, respectively). Glutathione-binding proteins were purified using glutathione affinity chromatography on protein from 5-day-old cells, corresponding to high GST activity (Fig. 1a) and the highest rate of anthocyanin accumulation in both treated and control cultures (data not shown). Control cultures, rather than treated cultures, were used for purification of GSTs in order to identify those proteins necessary and sufficient for anthocyanin transport. Multiple proteins were identified in the fractions displaying GST activity by one-dimensional gel electrophoresis (1D-GE; Fig. 1b). The GST activity of the fractions was proportional to the prevalence of proteins with a molecular weight of 21–26 kDa (Fig. 1b, c). The 45 kDa band, present in most fractions, may represent another glutathione-binding protein, with no apparent correlation with GST activity. GSTs were estimated to comprise >95% of protein in the bound fractions from the 1D-GE (equivalent to 2.3% of total protein from V. vinifera cell cultures).

Fig. 1.
Purification of glutathione-binding proteins from pigmented V. vinifera cell suspension cultures. (A) Vitis vinifera FU-01 cells were cultured in GC-2 medium (filled circles) for 4 d, then elicited with 10 μM jasmonic acid, 20 g ...
Fig. 5.
Anthocyanin accumulation and relative mRNA transcript levels for key anthocyanin biosynthetic genes in cultures following induction after 4 d with sucrose, jasmonic acid, and continuous light irradiation. (A) Anthocyanin content in V. vinifera ...

Two-dimensional gel electrophoresis and peptide mass fingerprinting

Fractions from the glutathione affinity chromatography with GST activity were pooled and separated by 2D-GE (Fig. 2). Proteins were visualized using the MS-compatible SYPRO Ruby stain, following verification that overexposed silver staining (data not shown) did not indicate the presence of otherwise undetected low abundance proteins.

Fig. 2.
(A) Two-dimensional gel electropherogram of glutathione affinity chromatography fractions possessing GST activity. (B) Enlarged image of the boxed region showing grouping based on mass spectrometry fingerprints (refer to Table 1). IEF strip pI3-10NL was ...
Table 1.
Peptide fingerprint analysis identifying the proteins as GSTs

Proteins were digested in-gel with trypsin, and a mass spectrum of the resulting peptides (a peptide mass fingerprint) was acquired with a matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometer as per Zhao et al. (2004). The list of apparent peptide masses was then aligned with predicted tryptic digests of known proteins by MASCOT and BLASTp (Altschul et al., 1990). A combination of low abundance and/or poor ionization did not enable the analysis of individual peptides by MS/MS.

This alignment enabled grouping of protein spots based on identical peptide fingerprints, with the variation in pI within each group a result of carbamylation of lysine residues diagnosed by the MS profile, a common artefact during the 2D separation procedures (McCarthy et al., 2003). Two groups (α, ε) required Edman sequencing to identify the N-terminus for stronger inference of the candidate expressed sequence tag (EST). The N-terminal sequence for group α did not enable robust degenerate PCR primer design, thus the gene designated as VvGST3 was obtained by 5′ RACE-PCR from its 3′ EST which had been identified by a single matching peptide fragment. The cloned gene matched the N-terminal sequence obtained by Edman degradation for this protein.

Once a contig was generated within The Institute of Genomic Research (TIGR) grape database, all GSTs were amplified from pigmented V. vinifera suspension cell cDNA. VvGST1, VvGST2, VvGST3, and VvGST4 all produced single PCR products of the predicted size, while Q84N22 yielded two PCR products. While the longer product possessed numerous stop codons in all frames, the shorter PCR product possessed 99% identity with the published Q84N22 sequence. Of note is a frameshift mutation common in all clones sequenced (AAA, instead of the published AAAA), altering all amino acids downstream of Leu45 and the predicted protein molecular weight for the Q84N22 EST from 15.7 kDa to 25.5 kDa. This agreed with the molecular weight of proteins purified from the cell culture (Figs 1, ,2),2), and therefore this coding sequence and protein are hereafter referred to as VvGST5.

Amplification of GST gene sequences from genomic DNA revealed the presence of a single intron in both VvGST1 and VVGST5, two introns in VvGST2 and VvGST3, while VvGST4 did not contain an intron (Fig. 3a). Alignments with other plant GST proteins indicate VvGST4 to be a type I GST (Fig. 3b) and support the classification of the other GSTs based on intron/exon structure alone.

Fig. 3.
(A) Genomic structure of V. vinifera GSTs cloned in this study. Exons are indicated by black arrows, and introns are indicated by double lines. (B) Phylogenetic tree of GSTs in the plant kingdom, showing classification into type I, II, and III GSTs as ...

Functional expression of genes encoding VvGSTs

Recombinant expression of these sequences was performed to confirm GST activity. Each cDNA clone was expressed in Escherichia coli with an N-terminal 6×HIS fusion to facilitate purification and western blotting. The fusion products were of the predicted size, and GST activity was confirmed in crude extracts from isopropyl-β-D-thiogalactopyranoside (IPTG)-induced E. coli for all genes (Fig. 4) and also following partial enrichment by Ni-NTA chromatography, showing activity similar to that of other recombinant GSTs cloned from poppy and petunia (data not shown; Alfenito et al., 1998; Yu and Facchini, 2000).

Fig. 4.
Western blot and GST activity performed on crude soluble protein extract from E. coli carrying vectors as indicated. –ve, E. coli carrying pQE9:VvGST5 without IPTG induction; p, E. coli carrying the pQE9 backbone only; GST coding sequences in ...

Transcription of GSTs and anthocyanin biosynthetic genes in cultured cells following induction with sucrose, jasmonic acid, and light irradiation

Treatment of V. vinifera cell suspension cultures with continuous white-light irradiation, sucrose, and jasmonic acid from day 4 increased the anthocyanin content (Fig. 5a) compared with control cultures at day 8. Jasmonic acid was the most effective single treatment, eliciting a 3.1-fold increase, while induction with all three treatments in combination yielded the maximum increase in anthocyanin content, ∼6-fold that in control cultures. Over the 96 h immediately following induction, distinct temporal patterns of transcript abundance for key anthocyanin biosynthetic genes (Fig. 5b) and putative GST genes (Fig. 5c) were observed in induced cultures, indicating differential regulation. Transcription of the first committed precursor pathway gene PAL increased to a maximum 12 h after induction, whereas transcripts for committed steps to flavonoids (CHS), leucocyanidins (DFR), and anthocyanins (UFGT) were co-ordinately up-regulated, with maxima at 24 h post-induction (Fig. 5b). The degree of up-regulation was comparable for each gene, within the range of 7- to 12-fold that in control cultures.

Under the induction conditions used here to enhance anthocyanin biosynthesis, all putative grape GST genes, with the exception of VvGST5, were up-regulated relative to control cultures (Fig. 5c). The greatest degree of up-regulation (12-fold) was observed for VvGST1, exhibiting a temporal pattern similar to PAL. Up-regulation of VvGST3 transcription also followed this pattern, although it was not as strongly induced (5.5-fold) over controls. The pattern of up-regulation for VvGST4 was similar to that of CHS, DFR, and UFGT, while VvGST2 exhibited delayed and sustained up-regulation of transcription (Fig. 5c).

Transcription of GST genes in grape berry skins at veráison

Bunches of V. vinifera L. cv. Shiraz berries were obtained from the same vine at veráison from predominantly green (<10% lightly pigmented berries, which were not chosen for extraction) and fully coloured bunches. The measure of total soluble solids for the green and red Shiraz berries was calculated as 6.4±0.3 °B and 14.8±1.4 °B, respectively. While no anthocyanin was detected by high-performance liquid chromatography (HPLC) from green berries, the red berry skins had an anthocyanin content of 1.4±0.05 mg g-FCW−1. These measurements justified the attribution of them as pre- and post-veráison stage berries (Kennedy et al., 2000).

Expression of VvGST4, VvGST3, and VvGST2 was found to be greater—60-, 2-, and 1.4-fold, respectively—in the post-veraison berry skins, concurrent with the accumulation of anthocyanin in this tissue (Fig. 6). In contrast, VvGST1 was the most strongly induced (∼13-fold) when suspension cell cultures were elicited to accumulate more anthocyanins. In grape berry skins, VvGST1 and VvGST5 expression was unaffected. Taken together, qPCR transcription analyses suggest a strong correlation of GST up-regulation with anthocyanin accumulation. Differences in the transcription profile may arise from interspecies variation, or a differential response to specific stimuli.

Fig. 6.
Relative mRNA transcript levels for GSTs in post-veraison V. vinifera L. cv. Shiraz berry skins compared with pre-veraison. Fold increase in steady-state transcripts calculated using the ΔΔ-Ct equation, with VvUbiquitin1 as internal control. ...

VvGST1 and VvGST4 are involved in anthocyanin accumulation in the vacuole

A complementation model for anthocyanin transport, based on reversion of the bz2 phenotype, was utilized to determine the ability of the putative V. vinifera L. GSTs to transport cytosolic anthocyanins to the vacuole. Each GST was transiently expressed in bz2 kernels to identify revertant zones (Fig. 7). These zones can be easily distinguished from the background and necrotic areas resulting from the high pressure bombardment due to the non-delimited anthocyanin deposition, appearing as a ‘halo’ of red pigmentation (Alfenito et al., 1998; Larsen et al., 2003). Of the grape GST genes tested, VvGST1 exhibited the greatest degree of bz2 complementation (37 zones mm−2, Table 2) and an average diameter of the revertant zones approaching that of Bz2, at 3.2 and 3.6 cell widths, respectively.

Table 2.
Quantitative complementation of Bz2 knockout corn seeds by V. vinifera GSTs
Fig. 7.
Complementation of anthocyanin transport by V. vinifera GSTs in Bronze-2-deficient corn kernels. Corn kernels 48 h after bombardment with GST constructs in pJD288 (35S) maize expression vector; 20× magnification. Bar = 300 μm. ...

VvGST4 was the only other GST tested to exhibit the complemented phenotype (10 zones mm−2) and a greatly reduced average diameter of these zones (2.3 cell widths, Table 2). All other VvGSTs and an unrelated GST from Arabidopsis thaliana possessed a background level of necrosis (Fig. 7) due to the bombardment, but no characteristic anthocyanin ‘halo’.

Conservation of protein residues found among plant GSTs is linked to the rescue of anthocyanin transport

Bestfit alignments were made between the amino acid sequence of all V. vinifera GSTs and those plant GSTs previously demonstrated to complement the bz2 mutant; An9, TT19, and Bz2 (Table 2; Marrs et al., 1995; Alfenito et al., 1998; Mueller et al., 2000). It was found that VvGST1 possessed the second highest similarity to Bz2 (48.2% similarity), while the most similar sequence, VvGST5 (48.3% similarity), did not complement bz2 in this assay. VvGST4 was most similar to An9 and TT19, 75.8% and 68.4% similarity, respectively. VvGST4 also gave a similar level of complementation in the maize bombardment assay to these GSTs (Alfenito et al., 1998), inferring that the protein sequence was influential in governing anthocyanin transport in the bz2 complementation model. All VvGST sequences were aligned with Bz2, TT19, ZmGSTIII, and An9 to identify conserved residues (Fig. 8). Few key regions were identified as being unique to anthocyanin-transporting GSTs, with other single or double amino acid residues differing between GSTs with low and high activity in the bz2 complementation assay.

Fig. 8.
Inference of functional residues in anthocyanin transport by protein modelling. Three-dimensional model of E. coli GST chain A, interacting with its substrate (glutathione sulphonic acid). The highlighted regions are within 3 Å of the ...

These regions were located on a three-dimensional model of two GSTs interacting with non-substrate ligands. The primary crystal structure used for this alignment was the E. coli GST chain A, complexed with the ligand glutathione sulphonic acid (Nishida et al., 1998) as the sequence was most similar to the VvGSTs, but sequences were also overlaid with the maize ZmGSTI complexed with lactoylglutathione (Neuefeind et al., 1997). Three of these homologous regions (ligand-binding sites, L-sites) were located on the outside of the protein between α-helices and within 3 Å, or in proximity for physical interaction with these ligands (Fig. 8).

There is a strong conservation in the core of these regions between VvGST1 and Bz2, while there are residues that differ from other anthocyanin-transporting GSTs. Also, residue differences between the highly similar VvGST5 and VvGST1 sequences fall in two of these regions (Fig. 8).

VvGST4, An9, and, to a lesser extent, ZmGSTIII (all type I GSTs) exhibit a similar response in the complementation assay (Alfenito et al., 1998). As well as high overall similarity, these GSTs possess strong homology in the aforementioned mini-regions, but differ from the GST1/Bz2 mini-regions and also for the non-transporting AtGSTF11. This includes high similarity within the glutathione-binding sites (G-sites) and L-sites.


Cloning of plant GSTs to date has predominantly been achieved through large-scale mutagenesis experiments, EST and differential display approaches, or genome sequencing projects such as with Arabidopsis, rice, and maize (McGonigle et al., 2000; Alonso et al., 2003). Affinity chromatography was used to purify two GSTs from oat seedlings, although they were only partially characterized; one GST from Z. mays was cloned via screening a cDNA library with antisera against the purified protein, and a single type III GST was cloned by degenerate PCR from opium poppy suspension cells (Dixon et al., 1998; Yu and Facchini, 2000; Zachariah et al., 2000). This is the first report of a proteomics approach for the purification and cloning of GSTs from any plant tissue. Anthocyanin-producing V. vinifera cv. Gamay Freaux cell suspension cultures were used to characterize anthocyanin transport proteins as they produce the same anthocyanin profile as in the berry, and for the ability to manipulate anthocyanin production in culture (Dornenburg and Knorr, 1996; Yeoman and Yeoman, 1996; Verpoorte et al., 1999).

The number of GST-like sequences found in different plant species ranges from 25 in soybean to 42 and 48 in maize and Arabidopsis, respectively (McGonigle et al., 2000; Dixon et al., 2002; Wagner et al., 2002). Pairoba and Walbot (2003) demonstrated that the 26 kDa Bz2 protein is accumulated only in anthocyanin-accumulating tissues, suggesting the presence of functional anthocyanin-specific GST protein in pigmented grape cells. Therefore, while this study cannot be considered exhaustive with regards to characterization of all the GSTs from V. vinifera, it is a comprehensive study of functional anthocyanin transport. Of the five GST sequences cloned in this study from pigmented suspension cells, there were three type I GSTs (VvGST2, VvGST3, and VvGST4) and two type III GSTs (VvGST1 and VVGST5) based on intron structure and protein sequence alignments.

Anthocyanin accumulation, including post-biosynthetic steps such as transport into the vacuole, has been shown in various systems to be regulated at the transcriptional level (Matsui et al., 2004; Hirai et al., 2005; Castellarin et al., 2006). It was hypothesized that if GSTs were involved in anthocyanin transport in V. vinifera, they would be present and transcriptionally up-regulated during anthocyanin synthesis. Using both V. vinifera suspension cells and grape berries, qPCR was employed to determine which of the five GSTs identified could be responsible for transporting anthocyanins in grape cells, through observation of co-regulation with anthocyanin biosynthetic genes and correlation to anthocyanin accumulation.

Biotic and abiotic elicitation is a common methodology for probing of metabolic pathways in in vitro systems, and a combination of jasmonic acid and light irradiation has been demonstrated to enhance anthocyanin biosynthesis in V. vinifera suspension cultures (Zhang et al., 2002b). High-sucrose stress, known to influence anthocyanin synthesis (Cormier et al., 1990; Solfanelli et al., 2006), in combination with jasmonic acid and light after 4 d of culture, enhanced anthocyanin accumulation >6-fold and enabled robust transcriptional profiles to be obtained for key anthocyanin biosynthetic genes. Differential regulation of PAL compared with later pathway genes was not unexpected. The most responsive GST gene to these conditions, VvGST1, also displayed a transcriptional profile similar to that of PAL, while the profile for VvGST4 was more similar to that of the late anthocyanin pathway genes, CHS, DFR, and UFGT, indicative of co-regulation. In orange fruit, the expression of a putative anthocyanin-transporting GST also correlated with the expression of the PAL, CHS, DFR, and UFGT genes under cold stress (Lo Piero et al., 2005, 2006).

Both C1 (Myb DNA-binding factor) and R (bHLH domain of Myc) transcription factor-binding elements reside in committed maize anthocyanin biosynthetic genes (Dooner et al., 1991). These elements have also been found in a 262 bp region of the Bz2 promoter, sufficient to confer co-ordinate regulation of this gene with those of the biosynthetic pathway (Bodeau and Walbot, 1992, 1996). Only VvGST1 possesses both of the the C1/R-binding motifs in its 5′-untranslated region. These elements may lead to the altered pattern of transcription seen in suspension cells versus grape berries.

There was no increase in VvGST5 in suspension cells, thus it was unlikely to be involved in anthocyanin transport under these conditions. Results with post-veráison berries provided further evidence that VvGST5 was not involved in anthocyanin transport, while the strongest up-regulation was for VvGST4, inferring a key role in anthocyanin transport in shiraz grapes. A similar pattern of induction was noted for numerous genes in Shiraz berries by Terrier et al. (2005), including a partial EST with high similarity to a GST gene (now known to correspond to VvGST4, cloned in this study). Interestingly, VvGST3 was the only other candidate that exhibited up-regulation in grape berries post-veráison. From the combined data, it appeared that VvGST1, VvGST3, and VvGST4 may be involved in anthocyanin transport, but in response to different stimuli—stress and grape berry ripening, respectively.

Complementation of anthocyanin transport in Bz2-deficient maize kernels was considered appropriate for inference of anthocyanin transport for VvGST genes as cyanidin 3-glucoside and its acylated derivatives are common anthocyanins in both plants (Cormier and Do, 1993). As in maize, two GST genes—VvGST1 and VvGST4—yielded positive results with this assay, although with different efficiencies.

Sequence analysis for all anthocyanin-transporting GSTs able to complement bz2 gave no consensus of substrate-binding residues (H-sites), supporting the findings of other researchers (Alfenito et al., 1998; Kitamura et al., 2004). However, both G-sites and L-sites on the exterior of the protein, thought to be prime candidate regions for binding non-substrate ligands, were shown to be conserved within the VvGST1/Bz2 and VvGST4/An9/ZmGSTIII groups, respectively. These regions were a source of dissimilarity between the otherwise similar VvGST1 and VvGST5, and may contribute to the lack of activity observed for VvGST5 in the maize complementation assay. All these critical binding sites were within 3 Å of the substrate. While ligandin-binding sites have been suggested to reside distinct from the active site (McTigue et al., 1995; Ji et al., 1996; Oakley et al., 1999), competitive activity has been demonstrated between model substrates (CDNB) and ligandins (anthocyanins, auxins), implicating the same binding site, i.e. the L-site (Mueller et al., 2000; Smith et al., 2003; Lo Piero et al., 2006).

VvGST1 possessed the second highest similarity to Bz2 behind the non-complementing VvGST5, and suggests the involvement of distinct residues in achieving anthocyanin transport. Interestingly, the similarity to Bz2 was proposed as the reason for the slightly higher complementation phenotype of ZmGSTIII as opposed to An9 (Alfenito et al., 1998). Certainly, the modification of individual flavonoids, by addition or translocation of a glucoside residue in cyanidin or luteolin, leads to a greatly reduced ability to bind An9 (Mueller et al., 2000).

Despite the dissimilarity of the dicot and monocot GST sequences, the strongest complementing GST in the dicot grape (VvGST1) is more similar to the monocot maize GST (Bz2) than any of the other transporting GSTs. The other V. vinifera GST capable of transport, VvGST4, possessed the highest similarity to the petunia An9 and yielded a similar result in the bz2 complementation assay (Alfenito et al., 1998). Maize and petunia have different dominant anthocyanin species, cyanidin and petunidin/cyanidin, respectively, with different GSTs required for transport being defined by unique consensus sequences inferred in this study. This may reflect the independent evolution of GSTs with regard to the anthocyanin profile within each species, with the prevalence of five anthocyanin compounds in grape requiring a second GST ligandin. In an evolutionary sense, plant GSTs belong to the most ancient class of GSTs, also present in vertebrates, Drosophila, and Methylobacterium (Mannervik and Danielson, 1988; Pemble and Taylor, 1992). This suggests that they are a representative of a progenitor GST arising before the divergence of prokaryotes and eukaryotes, maximizing the potential for independent gene duplication/sequence divergence to facilitate catalytic adaptation (Pemble and Taylor, 1992).

As is the case for many secondary metabolite biosynthetic proteins, there is an apparent redundancy with the anthocyanin-transporting GSTs in V. vinifera. This redundancy is seen with numerous functional copies of biosynthetic enzymes, including PAL (Ozeki et al., 1990), CHS (Reif et al., 1985; Durbin et al., 2000), and CHI (van Tunen et al., 1988); but also the putative anthocyanin transport pumps—ZmMRP3 and ZmMRP4 (Goodman et al., 2004). While maize also boasts two GSTs with transport activity, ZmGSTIII and Bz2, a knockout of the latter abrogates anthocyanin vacuolar deposition (Alfenito et al., 1998). This demonstrates a dependency on Bz2, yet does not eliminate the possibility of (i) ZmGSTIII transcriptional repression in these tissues, or (ii) potential heterodimerization with Bz2 for a functional ligandin. Furthermore, co-cultivation with abscisic acid induces vacuolar deposition of anthocyanins in bz2 maize kernels, supporting a partial redundancy by the auxin-inducible ZmGSTIII for Bz2, despite its reduced transport capacity (Walbot et al., 1994; Alfenito et al., 1998). Analysis of gene expression for ZmGSTIII in abscisic acid-treated bz2 kernels, or creation of ZmGSTIII knockout maize would be necessary to resolve this. Either of these models may also apply for VvGST1 and VvGST4 in V. vinifera.

Results presented in this study demonstrate the strong correlation between GST proteins, abundance of transcripts encoding them, and anthocyanin accumulation in V. vinifera. Specifically, VvGST1 and VvGST4 are the most responsive GSTs under synthetic (elicitation) and native (veráison) conditions increasing anthocyanin accumulation, respectively. In support of this, both VvGST1 and VvGST4 possess a capacity to complement for the loss of Bz2 in a maize anthocyanin transport complementation assay. Furthermore, the sequence conservation of specific residues in the GSTs contributes to their anthocyanin-transporting phenotype.

Understanding critical regions in the interaction, GSTs can be manipulated to alter or enhance accumulation of desired compounds, i.e. valuable flavonoids. Dixon et al. (2005) advocate the heterodimerization/co-expression, chimera formation, and random mutagenesis/recombination of maize GSTs for enhanced herbicide detoxification. Furthermore, overexpression of certain GSTs may enhance the accumulation of endogenous nutraceuticals, or GST repression may lead to enhanced accumulation of pytochelatins (heavy metal-binding proteins, reviewed in Dixon et al., 2005). These two opposing effects could result in a food source with greater nutrition in poor quality soils.

Supplementary data

Supplementary data for this manuscript detailing primers used for directional cloning of all GSTs and qPCR primers used can be accessed at JXB online.

Supplementary Material

[Supplementary Material]


The authors would like to thank Professor Virginia Walbot for supplying bronze-2 corn kernels, Drs Mark Alfenito and Dean Goodman for valuable assistance in complementation analysis, and Professor Antony Bacic and Dr Shaio-Lim Mau at the School of Botany, University of Melbourne for Edman sequencing. This work was supported by Flinders University and the Cooperative Research Centre for Bioproducts.


  • Aharoni A, Keizer LC, van den Broeck HC, Blanco-Portales R, Munoz-Blanco J, Bois G, Smit P, de Vos RC, O'Connell AP. Novel insight into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non-climacteric fruit. Plant Physiology. 2002;129:1019–1031. [PMC free article] [PubMed]
  • Alfenito MR, Souer E, Goodman CD, Buell R, Mol JNM, Koes R, Walbot V. Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases. The Plant Cell. 1998;10:1135–1149. [PMC free article] [PubMed]
  • Alonso JM, Stepanova AN, Leisse TJ, et al. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003;301:653–657. [PubMed]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. Journal of Molecular Biology. 1990;215:403–410. [PubMed]
  • Bézier A, Lambert B, Baillieul F. Study of defense-related gene expression in grapevine leaves and berries infected with Botrytis cinerea. European Journal of Plant Pathology. 2002;108:111–120.
  • Bilang J, Macdonald H, King PJ, Sturm A. A soluble auxin-binding protein from Hyoscyamus muticus is a glutathione S-transferase. Plant Physiology. 1993;102:29–34. [PMC free article] [PubMed]
  • Bilang J, Sturm A. Cloning and characterization of a glutathione S-transferase that can be photolabelled with 5-azido-indole-3-acetic acid. Plant Physiology. 1995;109:253–260. [PMC free article] [PubMed]
  • Bodeau JP, Walbot V. Regulated transcription of the maize Bronze-2 promoter in electroporated protoplasts requires the C1 and R gene products. Molecular and General Genetics. 1992;233:379–387. [PubMed]
  • Bodeau JP, Walbot V. Structure and regulation of the maize Bronze2 promoter. Plant Molecular Biology. 1996;32:599–609. [PubMed]
  • Bogs J, Ebadi A, McDavid D, Robinson SP. Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development. Plant Physiology. 2006;140:279–291. [PMC free article] [PubMed]
  • Buetler TM, Eaton DL. Glutathione S-transferases: amino acid sequence comparison, classification and phylogenetic relationship. Environmental Carcinogen and Ecotoxicology Reviews. 1992;C10:181–203.
  • Castellarin SD, Di Gaspero G, Marconi R, Nonis A, Peterlunger E, Paillard S, Adam-Blondon AF, Testolin R. Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3′-hydroxylase, flavonoid 3′,5′-hydroxylase genes and related metabolite profiling of red cyanidin/blue delphinidin-based anthocyanins in berry skin. BMC Genomics. 2006;24:12. [PMC free article] [PubMed]
  • Conn S, Zhang W, Franco CMM. Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture. Biotechnology Letters. 2003;25:835–839. [PubMed]
  • Conn VM, Walker AR, Franco CMM. Endophytic actinobacteria induce defense pathways in Arabidopsis thaliana. Molecular Plant-Microbe Interactions. 2008;21:208–218. [PubMed]
  • Cormier F, Crevier HA, Do CB. Effects of sucrose concentration on the accumulation of anthocyanins in grape (Vitis vinifera L.) cell suspension. Canadian Journal of Botany. 1990;68:1822–1825.
  • Cormier F, Do CB. XXVVII Vitis vinifera L (grapevine): in vitro production of anthocyanins. In: Bajaj YPS, editor. Medicinal and aromatic plants. Vol. 24. Berlin: Springer-Verlag; 1993. pp. 373–386.
  • Cormier F, Do CB, Nicolas Y. Anthocyanin production in selected cell lines of grape (Vitis vinifera L.) In Vitro Cellular and Developmental Biology of the Plant. 1994;30P:171–173.
  • Dean JD, Goodwin PH, Hsiang T. Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C. orbiculare and involvement of one in resistance. Journal of Experimental Botany. 2005;56:1525–1533. [PubMed]
  • Deprez RHL, Fijnvandraat AC, Ruijter JM, Moorman AFM. Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Analytical Biochemistry. 2002;307:63–69. [PubMed]
  • Dixon DP, Cole DJ, Edwards R. Purification, regulation, and cloning of a glutathione transferase (GST) from maize resembling the auxin-inducible type-III GSTs. Plant Molecular Biology. 1998;36:75–87. [PubMed]
  • Dixon DP, Lapthorn AJ, Edwards R. Plant glutathione transferases. Genome Biology. 2002;3:3001–3010.
  • Dixon DP, Lapthorn A, Edwards R. Synthesis and analysis of chimeric maize glutathione transferases. Plant Science. 2005;168:873–881.
  • Dooner HK, Robbins TP, Jorgensen RA. Genetic and developmental control of anthocyanin biosynthesis. Annual Review of Genetics. 1991;25:173–200. [PubMed]
  • Dornenburg H, Knorr D. Generation of colors and flavors in plant cell and tissue cultures. Critical Reviews in Plant Science. 1996;15:141–168.
  • Droog FNJ. Plant glutathione S-transferases, a tale of theta and tau. Journal of Plant Growth Regulation. 1997;16:95–107.
  • Droog FNJ, Hooykaas PJJ, Libbenga KR, van der Zaal EJ. Proteins encoded by an auxin-regulated gene family of tobacco share limited but functionally significant homology with glutathione S-transferases and one member indeed shows in vitro GST activity. Plant Molecular Biology. 1993;21:965–972. [PubMed]
  • Droog FNJ, Hooykaas PJJ, van der Zaal EJ. 2,4-Dichlorophenoxyacetic acid and related chlorinated compounds inhibit two auxin-regulated type III tobacco glutathione S-transferases. Plant Physiology. 1995;107:1139–1146. [PMC free article] [PubMed]
  • Durbin ML, McCaig B, Clegg MT. Molecular evolution of the chalcone synthase multigene family in the morning glory genome. Plant Molecular Biology. 2000;42:79–92. [PubMed]
  • Edwards R, Dixon DP. Plant glutathione transferases. Methods in Enzymology. 2005;401:169–186. [PubMed]
  • Fujiwara H, Tanaka Y, Yonekura-Sakakibara K, Fukuchi-Mizutani M, Nakao M, Fukui Y, Yamaguchi M, Ashikari T, Kusumi T. cDNA cloning, gene expression and subcellular localization of anthocyanin 5-aromatic acyltransferase from Gentiana triflora. The Plant Journal. 1998;16:421–431. [PubMed]
  • Gamborg O, Miller RA, Ojima K. Nutrient requirements of soybean root cells. Experimental Cellular Research. 1968;50:151–158. [PubMed]
  • Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RDB. Protein identification and analysis tools on the ExPASy server. In: Walker JM, editor. The proteomics protocols handbook. New York: Humana Press; 2005. pp. 571–607.
  • Goodman CD, Casati P, Walbot V. A multidrug-resistance associated protein involved in anthocyanin transport in Zea mays. The Plant Cell. 2004;16:1812–1826. [PMC free article] [PubMed]
  • Grotewold E. The genetics and biochemistry of floral pigments. Annual Reiews of Plant Biology. 2006;57:761–780. [PubMed]
  • Grotewold E, Chamberlin M, Snook M, Siame B, Butler L, Swenson J, Maddock S, St Clair G, Bowen B. Engineering secondary metabolism in maize cells by ectopic expression of transcription factors. The Plant Cell. 1998;10:721–740. [PMC free article] [PubMed]
  • Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry. 1974;249:7130–7139. [PubMed]
  • Hirai MY, Klein M, Fujikawa Y, et al. Elucidation of gene-to-gene and metabolite-to-gene networks in Arabidopsis by integration of metabolomics and transcriptomics. Journal of Biological Chemistry. 2005;280:25590–25595. [PubMed]
  • Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gaillard GL. Location of a potential transport binding site in a sigma class glutathione transferase by X-ray crystallography. Proceedings of the National Academy of Sciences, USA. 1996;93:8208–8213. [PMC free article] [PubMed]
  • Jones AM. Auxin-binding proteins. Annual Review of Plant Physiology and Plant Molecular Biology. 1994;45:393–420.
  • Jonsson LMV, Donker-Koopman WE, Uitslager P, Schram AW. Subcellular localization of anthocyanin methyltransferase in flowers of Petunia hybrida. Plant Physiology. 1983;72:287–290. [PMC free article] [PubMed]
  • Kennedy JA, Troup GJ, Pilbrow JR, Hutton DR, Hewitt D, Hunter CR, Ristic R, Iland PG, Jones GP. Development of seed polyphenols in berries from Vitis vinifera L. cv. Shiraz. Australian Journal of Grape and Wine Research. 2000;6:244–254.
  • Kitamura S, Shikazono N, Tanaka A. TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis. The Plant Journal. 2004;37:104–114. [PubMed]
  • Larsen ES, Alfenito MR, Briggs WR, Walbot V. A carnation anthocyanin mutant is complemented by the glutathione S-transferases encoded by maize Bz2 and petunia An9. Plant Cell Reports. 2003;21:900–904. [PubMed]
  • Lederer B, Böger P. A ligand function of glutathione S-transferase. Zeitschrift für Naturforschung. 2005;60c:166–171. [PubMed]
  • Lo Piero AR, Puglisi I, Petrone G. Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)] Journal of Agricultural Food Chemistry. 2006;54:9227–9233. [PubMed]
  • Lo Piero AR, Puglisi I, Rapisarda P, Petrone G. Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. Journal of Agricultural Food Chemistry. 2005;53:9083–9088. [PubMed]
  • Luehrsen K, DeWet J, Walbot V. Use of luciferase as a reporter gene. Methods in Enzymology. 1992;216:397–414. [PubMed]
  • Mannervik B, Danielson UH. Glutathione transferases: structure and catalytic activity. CRC Critical Reviews in Biochemistry. 1988;23:283–337. [PubMed]
  • Marrs KA. The functions and regulation of glutathione S-transferases in plants. Annual Reviews in Plant Physiology and Plant Molecular Biology. 1996;47:127–158. [PubMed]
  • Marrs KA, Alfenito MR, Lloyd AM, Walbot V. A glutathione S-transferase involved in vacuolar transfer encoded by the maize gene Bronze-2. Nature. 1995;375:397–400. [PubMed]
  • Matsui K, Tanaka H, Ohme-Takagi M. Suppression of the biosynthesis of proanthocyanidin in Arabidopsis by a chimeric PAP1 repressor. Plant Biotechnology Journal. 2004;2:487–493. [PubMed]
  • McCarthy J, Hopwood F, Oxley D, Laver M, Castagna A, Righetti PG, Williams K, Herbert B. Carbamylation of proteins in 2-D electrophoresis—myth or reality? Journal of Proteome Research. 2003;2:239–242. [PubMed]
  • McGonigle B, Keeler S, Lau S-M, Koeppe M, O'Keefe D. A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize. Plant Physiology. 2000;124:1105–1120. [PMC free article] [PubMed]
  • McTigue MA, Williams DR, Trainer JA. Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel. Journal of Molecular Biology. 1995;246:21–27. [PubMed]
  • Mueller LA, Goodman CD, Silady RA, Walbot V. AN9, a petunia glutathione S-transferase required for anthocyanin sequestration, is a flavonoid-binding protein. Plant Physiology. 2000;123:1561–1570. [PMC free article] [PubMed]
  • Mueller LA, Walbot V. Models for vacuolar sequestration of anthocyanins. Recent Advances in Phytochemistry. 2001;35:291–312.
  • Neuefeind T, Huber R, Dasenbrock H, Prade L, Bieseler B. Crystal structure of herbicide-detoxifying maize glutathione S-transferase-I in complex with lactoylglutathione: evidence for an induced-fit mechanism. Journal of Molecular Biology. 1997;274:446–453. [PubMed]
  • Nishida M, Harada S, Noguchi S, Satow Y, Inoue H, Takahashi K. Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106. Journal of Molecular Biology. 1998;281:135–147. [PubMed]
  • Oakley AJ, Bello ML, Nuccetelli M, Mazzetti AP, Parker MW. The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site) Journal of Molecular Biology. 1999;291:913–926. [PubMed]
  • Ozeki Y, Matsui K, Sakuta M-a, Matsuoka M, Ohashi Y, Kano-Murakami Y, Yamamoto N, Tanaka Y. Differential regulation of phenylalanine ammonia-lyase genes during anthocyanin synthesis and by transfer effect in carrot cell suspension cultures. Physiologia Plantarum. 1990;80:379–387.
  • Pairoba CF, Walbot V. Post-transcriptional regulation of expression of the Bronze-2 gene of Zea mays L. Plant Molecular Biology. 2003;53:75–86. [PubMed]
  • Pecket RC, Small CJ. Occurrence, location and development of anthocyanoplasts. Phytochemistry. 1980;19:2571–2576.
  • Pemble SE, Taylor JB. An evolutionary perspective on glutathione transferases inferred from class-Theta glutathione transferase cDNA sequences. Biochemistry Journal. 1992;287:957–963. [PMC free article] [PubMed]
  • Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research. 2001;29:2002–2007. [PMC free article] [PubMed]
  • Reif HJ, Niesbach U, Deumling B, Saedler H. Cloning and analysis of two genes for chalcone synthase from Petunia hybrida. Molecular and General Genetics. 1985;199:208–215.
  • Sambrook JF, Maniatis T. Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989.
  • Smith AP, Nourizadeh SD, Peer WA, Xu J, Bandyopadhyay A, Murphy AS, Goldsbrough PB. Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids. The Plant Journal. 2003;36:433–442. [PubMed]
  • Solfanelli C, Poggi A, Loreti E, Alpi A, Perata P. Sucrose-specific induction of anthocyanin biosynthetic pathway in Arabidopsis. Plant Physiology. 2006;140:637–646. [PMC free article] [PubMed]
  • Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C. Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.) Plant Molecular Biology. 1994;24:743–755. [PubMed]
  • Takahashi Y, Ishida S, Nagata T. Auxin-regulated genes. Plant and Cell Physiology. 1995;36:383–390.
  • Terrier N, Glissant D, Grimplet J, et al. Isogene specific oligo arrays reveal multifaceted changes in gene expression during grape berry (Vitis vinifera L.) development. Planta. 2005;222:832–847. [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research. 1994;22:4673–4680. [PMC free article] [PubMed]
  • Ulmasov T, Hagen G, Guilfoyle T. The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues. Plant Molecular Biology. 1994;26:1055–1064. [PubMed]
  • Ulmasov T, Ohmiya A, Hagen G, Guilfoyle T. The soybean GH214 gene that encodes a glutathione S-transferase has a promoter that is activated by a wide range of chemical agents. Plant Physiology. 1995;108:919–927. [PMC free article] [PubMed]
  • van Tunen AJ, Koes RE, Spelt CE, van der Krol AR, Stuitje AR, Mol JN. Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes. EMBO Journal. 1988;7:1257–1263. [PMC free article] [PubMed]
  • Verpoorte R, van der Heijden R, ten Hoopen HJG, Memelink J. Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnology Letters. 1999;21:467–479.
  • Wagner U, Edwards R, Dixon DP, Mauch F. Probing the diversity of the Arabidopsis glutathione S-transferase gene family. Plant Molecular Biology. 2002;49:515–532. [PubMed]
  • Walbot V, Benito M-I, Bodeau J, Nash J. Abscisic acid induces pink pigmentation in maize aleurone tissue in the absence of Bronze-2. Maydica. 1994;39:19–28.
  • Wilkins MR, Lindskog I, Gasteiger E, Bairoch A, Sanchez J-C, Hochstrasser DF, Appel RD. Detailed peptide characterisation using PEPTIDEMASS—a World-Wide Web accessible tool. Electrophoresis. 1997;18:403–408. [PubMed]
  • Winefield C, Cartwright J, Mitchell N, Trought M, Jordan B. Proceedings of the 8th International Congress of Plant Molecular Biology. Adelaide, Australia: 2006. Characterisation of the biochemical pathway responsible for the formation of glutathione–aroma compound conjugates from Sauvignon blanc grapes.
  • Winkel BSJ. Metabolic channeling in plants. Annual Review of Plant Physiology Plant Molecular Biology. 2004;55:85–107. [PubMed]
  • Yeoman MM, Yeoman CL. Manipulating secondary metabolism in cultured plant cells. New Phytologist. 1996;134:553–569.
  • Yonekura-Sakakibara K, Tanaka Y, Fukuchi-Mizutani M, Fujiwara H, Fukui Y, Ashikari T, Murakami Y, Yamaguchi M, Kusumi T. Molecular and biochemical characterization of a novel hydroxycinnamoyl-CoA:anthocyanin 3-O-glucoside-6′′-O-acyltransferase from Perilla frutescens. Plant and Cell Physiology. 2000;41:495–502. [PubMed]
  • Yu M, Facchini PJ. Molecular cloning and characterization of a type III glutathione S-transferase from cell suspension cultures of opium poppy treated with a fungal elicitor. Physiologia Plantarum. 2000;108:101–109.
  • Zachariah VT, Walsh-Sayles N, Singh BR. Isolation, purification, and characterization of glutathione S-transferase from oat (Avena sativa) seedlings. Journal of Protein Chemistry. 2000;19:425–430. [PubMed]
  • Zhang W, Curtin C, Franco C. Towards manipulation of post-biosynthetic events in secondary metabolism of plant cell cultures. Enzyme and Microbial Technology. 2002a;30:688–696.
  • Zhang W, Curtin C, Kikuchi M, Franco C. Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Science. 2002b;162:459–468.
  • Zhang W, Franco C, Curtin C, Conn S. To stretch the boundary of secondary metabolite production in plant cell-based bioprocessing: anthocyanin as a case study. Journal of Biomedicine and Biotechnology. 2004;5:264–271. [PMC free article] [PubMed]
  • Zhao Z, Raftery MJ, Niu XM, Daja MM, Russell PJ. Application of in-gel protease assay in a biological sample: characterization and identifacation of urokinase-type plasminogen activator (uPA) in secreted proteins from a prostate cancer cell line PC-3. Electrophoresis. 2004;25:1142–1148. [PubMed]

Articles from Journal of Experimental Botany are provided here courtesy of Oxford University Press
PubReader format: click here to try


Save items

Related citations in PubMed

See reviews...See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • MedGen
    Related information in MedGen
  • Nucleotide
    Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • Protein
    Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
  • Taxonomy
    Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...