Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. May 1985; 54(2): 460–466.
PMCID: PMC254817

Isolation and characterization of the bacteriophage T4 tail-associated lysozyme.

Abstract

Direct evidence has been obtained that the tail-associated lysozyme of bacteriophage T4 (tail-lysozyme) is gp5, which is a protein component of the hub of the baseplate. Tails were treated with 3 M guanidine hydrochloride containing 1% Triton X-100, and the tail-lysozyme was separated from other tail components by preparative isoelectric focusing electrophoresis as a peak with a pI of 8.4. The molecular weight as determined from sodium dodecyl sulfate electrophoresis was 42,000. The tail-lysozyme was unambiguously identified as gp5 when the position of the lysozyme was compared with that of gp5 of tube-baseplates from 5ts1/23amH11/eL1ainfected Escherichia coli cells by two-dimensional gel electrophoresis. The tail-lysozyme has N-acetylmuramidase activity and the same substrate specificity as gene e lysozyme; the optimum pH is around 5.8, about 1 pH unit lower than for the e lysozyme. We assume that the tail-lysozyme plays an essential role in locally digesting the peptidoglycan layer to let the tube penetrate into the periplasmic space. The tail-lysozyme is presumably also responsible for "lysis from without."

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Black LW, Hogness DS. The lysozyme of bacteriophage lambda. I. Purification and molecular weight. J Biol Chem. 1969 Apr 25;244(8):1968–1975. [PubMed]
  • Crowther RA, Lenk EV, Kikuchi Y, King J. Molecular reorganization in the hexagon to star transition of the baseplate of bacteriophage T4. J Mol Biol. 1977 Nov 5;116(3):489–523. [PubMed]
  • Duda RL, Eiserling FA. Evidence for an internal component of the bacteriophage T4D tail core: a possible length-determining template. J Virol. 1982 Aug;43(2):714–720. [PMC free article] [PubMed]
  • Fairbanks G, Steck TL, Wallach DF. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. [PubMed]
  • Furukawa H, Kuroiwa T, Mizushima S. DNA injection during bacteriophage T4 infection of Escherichia coli. J Bacteriol. 1983 May;154(2):938–945. [PMC free article] [PubMed]
  • Iba H, Nanno M, Okada Y. Identification and partial purification of a lytic enzyme in the bacteriophage phi 6 virion. FEBS Lett. 1979 Jul 15;103(2):234–237. [PubMed]
  • Iwashita S, Kanegasaki S. Smooth specific phage adsorption: endorhamnosidase activity of tail parts of P22. Biochem Biophys Res Commun. 1973 Nov 16;55(2):403–409. [PubMed]
  • Kao SH, McClain WH. Baseplate protein of bacteriophage T4 with both structural and lytic functions. J Virol. 1980 Apr;34(1):95–103. [PMC free article] [PubMed]
  • KATZ W, WEIDEL W. [Isolation and characterization of the lysozyme bound to T2 phage]. Z Naturforsch B. 1961 Jun;16B:363–368. [PubMed]
  • Kells SS, Haselkorn R. Bacteriophage T4 short tail fibers are the product of gene 12. J Mol Biol. 1974 Mar 15;83(4):473–485. [PubMed]
  • Labedan B, Goldberg EB. Requirement for membrane potential in injection of phage T4 DNA. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4669–4673. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Loeb MR. Bacteriophage T4-mediated release of envelope components from Escherichia coli. J Virol. 1974 Mar;13(3):631–641. [PMC free article] [PubMed]
  • Moody MF. Sheath of bacteriophage T4. 3. Contraction mechanism deduced from partially contracted sheaths. J Mol Biol. 1973 Nov 15;80(4):613–635. [PubMed]
  • Oakley BR, Kirsch DR, Morris NR. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. [PubMed]
  • O'Farrell PZ, Goodman HM, O'Farrell PH. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. [PubMed]
  • PARK JT, JOHNSON MJ. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed]
  • Simon LD, Anderson TF. The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. II. Structure and function of the baseplate. Virology. 1967 Jun;32(2):298–305. [PubMed]
  • Szewczyk B, Skórko R. Lysozyme activity of bacteriophage T4 ghosts. Biochim Biophys Acta. 1981 Nov 13;662(1):131–137. [PubMed]
  • Szewczyk B, Skórko R. Purification and some properties of bacteriophage T4 particle-associated lysozyme. Eur J Biochem. 1983 Jul 1;133(3):717–722. [PubMed]
  • Tschopp J, Arisaka F, van Driel R, Engel J. Purification, characterization and reassembly of the bacteriophage T4D tail sheath protein P18. J Mol Biol. 1979 Feb 25;128(2):247–258. [PubMed]
  • Tsugita A, Inouye M. Purification of bacteriophage T4 lysozyme. J Biol Chem. 1968 Jan 25;243(2):391–397. [PubMed]
  • Tsukagoshi N, Schäfer R, Franklin RM. Structure and synthesis of a lipid-containing bacteriophage. An endolysin activity associated with bacteriophage PM2. Eur J Biochem. 1977 Aug 1;77(3):585–588. [PubMed]
  • Wyckoff M, Rodbard D, Chrambach A. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate-containing buffers using multiphasic buffer systems: properties of the stack, valid Rf- measurement, and optimized procedure. Anal Biochem. 1977 Apr;78(2):459–482. [PubMed]
  • Yamamoto M, Uchida H. Organization and function of bacteriophage T4 tail. I. Isolation of heat-sensitive T4 tail mutants. Virology. 1973 Mar;52(1):234–245. [PubMed]
  • Zorzopulos J, Kozloff LM, Chapman V, DeLong S. Bacteriophage T4D receptors and the Escherichia coli cell wall structure: role of spherical particles and protein b of the cell wall in bacteriophage infection. J Bacteriol. 1979 Jan;137(1):545–555. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...