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J Virol. 1984 Dec; 52(3): 892–896.
PMCID: PMC254610

Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.


Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions.

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Selected References

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  • Barrell BG, Air GM, Hutchison CA., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. [PubMed]
  • Dowell CE. Cold-sensitive mutants of bacteriophage phi-X-174. I. A mutant blocked in the eclipse function at low temperature. Proc Natl Acad Sci U S A. 1967 Sep;58(3):958–961. [PMC free article] [PubMed]
  • Dunn JJ, Buzash-Pollert E, Studier FW. Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741–2745. [PMC free article] [PubMed]
  • Gillam S, Astell CR, Smith M. Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency. Gene. 1980 Dec;12(1-2):129–137. [PubMed]
  • Gillam S, Jahnke P, Astell C, Phillips S, Hutchison CA, 3rd, Smith M. Defined transversion mutations at a specific position in DNA using synthetic oligodeoxyribonucleotides as mutagens. Nucleic Acids Res. 1979 Jul 11;6(9):2973–2985. [PMC free article] [PubMed]
  • Gillam S, Jahnke P, Smith M. Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. J Biol Chem. 1978 Apr 25;253(8):2532–2539. [PubMed]
  • Gillam S, Smith M. Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum ologodeoxyribonucleotide length. Gene. 1979 Dec;8(1):81–97. [PubMed]
  • Gold L, Pribnow D, Schneider T, Shinedling S, Singer BS, Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. [PubMed]
  • Hutchison CA, 3rd, Phillips S, Edgell MH, Gillam S, Jahnke P, Smith M. Mutagenesis at a specific position in a DNA sequence. J Biol Chem. 1978 Sep 25;253(18):6551–6560. [PubMed]
  • Hutchison CA, 3rd, Sinsheimer RL. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. [PubMed]
  • Pollock TJ, Tessman ES, Tessman I. Identification of lysis protein E of bacteriophage phiX174. J Virol. 1978 Oct;28(1):408–410. [PMC free article] [PubMed]
  • Sanger F, Coulson AR. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. [PubMed]
  • Sanger F, Coulson AR, Friedmann T, Air GM, Barrell BG, Brown NL, Fiddes JC, Hutchison CA, 3rd, Slocombe PM, Smith M. The nucleotide sequence of bacteriophage phiX174. J Mol Biol. 1978 Oct 25;125(2):225–246. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Schnegg B, Hofschneider PH. Mutant of phi X174 accessible to host-controlled modification. J Virol. 1969 May;3(5):541–542. [PMC free article] [PubMed]
  • Shine J, Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. [PMC free article] [PubMed]
  • Singer BS, Gold L, Shinedling ST, Colkitt M, Hunter LR, Pribnow D, Nelson MA. Analysis in vivo of translational mutants of the rIIB cistron of bacteriophage T4. J Mol Biol. 1981 Jul 5;149(3):405–432. [PubMed]
  • Steitz JA, Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734–4738. [PMC free article] [PubMed]

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