• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jphysiolThe Journal of Physiology SiteMembershipSubmissionJ Physiol
J Physiol. Aug 1, 2008; 586(Pt 15): 3565–3575.
Published online Jun 5, 2008. doi:  10.1113/jphysiol.2008.155812
PMCID: PMC2538826

TRP channels entering the structural era


Transient receptor potential (TRP) channels are important in many neuronal and non-neuronal physiological processes. The past 2 years have seen much progress in the use of structural biology techniques to elucidate molecular mechanisms of TRP channel gating and regulation. Two approaches have proven fruitful: (i) a divide-and-conquer strategy has provided high-resolution structural details of TRP channel fragments although it fails to explain how these fragments are integrated in the full channel; and (ii) electron microscopy of entire TRP channels has yielded low-resolution images that provide a basis for testable models of TRP channel architecture. The results of each approach, summarized in this review, provide a preview of what the future holds in TRP channel structural biology.

Transient receptor potential (TRP) channels form a diverse family of cation channels that respond to a variety of signals (Ramsey et al. 2006; Venkatachalam & Montell, 2007). For example, some are involved in sensory perception and are directly activated by chemical ligands and/or physical sensory stimuli such as temperature, mechanical and osmotic stresses. Others are activated downstream of receptor stimulation through a phospholipase C (PLC)-dependent pathway. An intriguing feature shared by several TRP channels is that a given TRP channel gating response is the result of the integration of several signals of different nature (chemical or physical) and different sources (intra- or extracellular) (O'Neil & Brown, 2003; Soboloff et al. 2007). TRP channels are particularly prominent in the genomes of the animal kingdom. In mammals, the TRP family contains nearly 30 members distributed into six subfamilies according to sequence and function (Montell, 2005): TRPA (ankyrin), TRPC (canonical), TRPM (melastatin), TRPML (mucolipin), TRPP (polycystin) and TRPV (vanilloid). An additional subfamily, TRPN (NOMPC), is absent in mammals but found in many other organisms including worms and fish.

TRP proteins have six transmembrane segments homologous to the transmembrane domain of Shaker potassium channels (Long et al. 2005, 2007). Just like Shaker channels, TRP proteins also assemble as tetrameric channels, as demonstrated by several biochemical studies (e.g. Kedei et al. 2001; Phelps & Gaudet, 2007) and, very convincingly, by atomic force microscopy studies on TRPC1 (Barrera et al. 2007). Major differences between TRP channel subfamilies lie in the large N- and C-terminal cytosolic domains which contain putative protein interaction and regulatory motifs and have distinct features in different TRP subfamilies. Figure 1 illustrates the distinct sequence features of each TRP channel subfamily. Ankyrin repeats are present in the N-terminal cytosolic region of TRPC, TRPV, TRPA and TRPN channels. While the TRPC and TRPV channels have few repeats and irregular sequences (Phelps et al. 2007, 2008), TRPA and TRPN have many regular repeats (see Gaudet, 2008, for a recent review). TRPM channels also have a large, ~700-residue N-terminal intracellular region, which can be subdivided in four subdomains labelled ‘TRPM homology regions’ or MHRs, with similarity only to other TRPM channels (Clapham, 2003; Fleig & Penner, 2004). In their C-terminal intracellular region, TRPM channels have a coiled-coil region (Jenke et al. 2003; Montell, 2005). A few TRPM proteins also have a large extension of the C-terminal intracellular region beyond the coiled-coil region, encoding an enzymatic domain (Cahalan, 2001): TRPM6 and TRPM7 have a C-terminal α-kinase domain (Nadler et al. 2001; Riazanova et al. 2001; Runnels et al. 2001), and TRPM2 has a C-terminal NUDIX domain (Perraud et al. 2001). Finally, both TRPP and TRPML channels have an extracellular domain inserted between transmembrane segments S1 and S2, although there is no significant sequence similarity between the extracellular domains of TRPP and TRPML proteins.

Figure 1
Primary structures of the seven TRP channel subfamilies

Until 2 years ago, three-dimensional structure information on TRP channels was largely limited to structures of homologous domains from other proteins (Gaudet, 2006), aside from the crystal structure of the TRPM7 α-kinase domain (Yamaguchi et al. 2001), a domain unique to TRPM6 and TRPM7. However, TRP channels are now entering the structural era. Here I will introduce some of the methodologies available and approaches to TRP channel structural biology, review the recent literature on TRP channel structure, and discuss some of the challenges that lie ahead.

Structural biology of TRP channels

There are three major techniques to obtain structural information on macromolecules: X-ray crystallography, nuclear magnetic resonance (NMR) and electron microscopy (EM) – either single particle EM or electron crystallography. A recent primer on structural biology for neuroscientists is an excellent source of information on these methods (Minor, 2007). Two factors make structural studies of TRP channels a particularly difficult challenge for structural biologists. First, structural biology techniques require an ample supply of highly pure and stable protein samples, and membrane proteins are notoriously difficult to produce in large quantities and purify in a stable native state (see Grisshammer, 2006, for a recent review). Second, as introduced above, TRP channels are very large tetramers of 70–250 kDa subunits that are each formed of multiple domains (structural biologists usually define a protein ‘domain’ as an independently folding unit, a protein segment that can take on its native conformation even when isolated from the rest of the protein). Such large proteins are currently inaccessible to high-resolution solution NMR techniques. In addition, the flexibility afforded by multiple domains, which probably has functional significance, often hinders crystallization – a necessary step in X-ray crystallographic analyses – because crystals are formed of an ordered array of molecules in an identical conformation. Flexibility also complicates single particle EM studies because the sample is then a heterogeneous mixture of distinct conformations, which have to be classified properly and averaged independently.

To compensate for the dearth of structural information on TRP channels, a number of studies have made use of various modelling approaches to provide a context for their mutagenesis data. These can be quite powerful especially when the structures of close structural homologues are available. In that respect, the structures of potassium channels like KcsA (Doyle et al. 1998) – whose structure is expected to be similar to the pore domain of TRP channels, and the Shaker channels Kv1.2 and Kv2.1 (Long et al. 2005, 2007) can be helpful. One example where a structural model was used to design and interpret experiments on a TRP channel is a mutagenesis study of the outer pore structure and ion selectivity of the calcium-dependent TRPV6 channel (Voets et al. 2004). However, this review will focus on experimental structure determination results rather than modelling.

Two approaches have now yielded information on the structure of TRP channels. On one hand, high-resolution structures and other biophysical measurements of isolated cytosolic domains of several TRP channels have yielded insights into their biological function. On the other hand, several single-particle EM studies provide a low-resolution view of entire TRP channels. The results available for each approach are summarized in the next two sections.

Divide and conquer: structures of TRP channel domains

Although the functional differences of various TRP channels are probably due in large part to their strikingly diverse cytosolic domains, their sequences reveal little about their structure and function. Determining the three-dimensional structure and functional properties of these domains is critical to deciphering their role in TRP channel function and regulation. This divide-and-conquer approach has yielded structural information on three types of TRP channel domains: (i) several structures of the ankyrin repeat domains of TRPV channels; (ii) structural information on the coiled-coil domain of TRPM channels; and (iii) a structure of the α-kinase domain of TRPM7. These three cases are described below.

Ankyrin repeats, found in the N-terminal cytosolic segments of TRPA, TRPC, TRPN and TRPV channels, are identifiable in protein sequences as ~33-residue conserved sequence motifs found in tandem arrays ranging from 3 to more than 30 repeats (Mosavi et al. 2004). Figure 2A shows the structure of a set of six ankyrin repeats as a ribbon diagram. The structure of each ankyrin repeat consists of a hairpin of two short α-helices, followed by a hairpin loop that projects out perpendicular to the helical axes. Tandem repeats then stack together side-by-side so that the first helices in each repeat – termed inner helices – form a concave surface, while the second, outer helices, form a convex surface. The concave surface formed by the inner helices is adjacent to the hairpin loops, and they are often referred to as the palm and fingers, respectively, of a hand-shaped domain. This analogy is particularly useful because most ligand interactions are observed on these surfaces, as if the hand grabs onto the ligand. The only known biochemical function of ankyrin repeat domains is to interact with ligands, although the nature of ligands is highly diverse, from small molecules to unusual oligonucleotides and large proteins (Myers & Julius, 2007; Gaudet, 2008).

Figure 2
High-resolution structures of TRP channel fragments

To date, the structural data on TRP channel ankyrin repeats are restricted to the TRPV subfamily. TRPV channels are involved in pain, thermo- and mechanosensation, and calcium homeostasis (Niemeyer, 2005). In mammals there are six TRPV channels that partition into two groups: TRPV1–4, involved in sensory signalling; and the more distantly related TRPV5 and TRPV6, expressed in the intestinal tract and kidneys and important for calcium homeostasis. The structures of the N-terminal ankyrin repeat domain of three TRPV channels, TRPV1, TRPV2 and TRPV6, have been published (Jin et al. 2006; McCleverty et al. 2006; Lishko et al. 2007; Phelps et al. 2008). At a superficial level, the structures are very similar (Fig. 2A), as expected from their sequence homology. The TRPV ankyrin repeat domains have six ankyrin repeats, with unusually long finger loops (especially the first three loops). Amino acid residues that are conserved in TRPV proteins but diverge from the ankyrin repeat sequence consensus cause a pronounced twist between the fourth and fifth repeat (Phelps et al. 2008). Since these residues are conserved in TRPV proteins, the overall shape of the TRPV ankyrin repeats is likely to be conserved in all TRPV family members. Of note, sequence analyses indicate that the ankyrin repeats from other TRP channel subfamilies are only distantly related to the TRPV ankyrin repeats, and therefore imply that their structures will also be quite different (Phelps et al. 2007).

The TRPV ankyrin repeat structures do have notable differences in their details. More importantly, the structural differences result in drastically different biochemical properties. For instance, the structure of the TRPV1 ankyrin repeats shows a bound ATP molecule (Fig. 2B), and accompanying data support a role for this ATP-binding site in regulating TRPV1 sensitivity (Lishko et al. 2007). TRPV1 is expressed in nociceptor neurons and is activated by heat and capsaicin, the pungent compound causing the ‘hot’ taste of chilli peppers (Caterina et al. 1997). Biochemical and electrophysiology experiments showed that intracellular ATP can sensitize the TRPV1 response to capsaicin, whereas calcium-bound calmodulin, acting through the same binding region on the TRPV1 ankyrin repeats, causes desensitization (Lishko et al. 2007). This ATP-binding site is conserved in TRPV1 from other species (Phelps et al. 2007), more so than the capsaicin-binding site, which is not conserved in chicken TRPV1, for instance (Jordt & Julius, 2002). However, this ATP-binding site is not conserved in several other TRPV channels, including TRPV2, a close homologue of TRPV1, also involved in sensing hot temperatures, and TRPV5 or TRPV6, involved in calcium homeostasis (Lishko et al. 2007; Phelps et al. 2008). Similarly, the TRPV1 ankyrin repeats bind to calmodulin, as do – although weakly – the TRPV5 ankyrin repeats, whereas the TRPV2 and TRPV6 ankyrin repeats do not (Lishko et al. 2007; Phelps et al. 2008). These structural and biochemical studies demonstrate that studying isolated domains can provide useful information about a domain's function in the context of the whole ion channel, and that high-resolution information provides the level of detail required to grasp some of the unique features of each channel.

Most TRPV channels have 50–150 amino acid residues of poorly conserved sequence N-terminal to the six ankyrin repeats. An N-terminal deletion analysis on TRPV1 indicated that removing the ~100 residues N-terminal to the ankyrin repeats had little effect on its electrophysiological properties (Jung et al. 2002). Furthermore, although the protein crystals used to determine the structure of human TRPV2 ankyrin repeats also included the 68 residues N-terminal to the ankyrin repeats, the resulting X-ray diffraction data provided no interpretable electron density corresponding to these 68 residues, implying that they are flexible and disordered (McCleverty et al. 2006). In this case the combination of functional and structural data suggests a flexible N-terminal appendage that may not be important in channel function. In TRPV4, the junction between the N-terminal region and the ankyrin repeats is proline-rich and interacts with the Src Homology 3 (SH3) domain of PACSIN3 (Cuajungco et al. 2006). The TRPV4–PACSIN3 interaction selectively inhibits TRPV4 basal activity and activation by cell swelling and heat, but not its activation by phorbol ligands (D'Hoedt et al. 2008). SH3 domains typically interact with a proline-rich peptide segment in an extended polyproline type II helix (Musacchio, 2002), which fits with at least the linker between the N-terminal region and ankyrin repeats of TRPV4 being flexible.

The TRPV ankyrin repeat structures can also provide a useful perspective on available functional data. For instance, TRPV1 activation by allicin, the pungent compound in garlic, has been attributed to the covalent modification of cysteine 157 in the N-terminal ankyrin repeat domain (Salazar et al. 2008). The sulfhydryl group of C157 is located in close proximity to the regulatory ATP- and calmodulin-binding region, although it is completely buried between repeats 1 and 2 of the structure (Fig. 2C). Salazar and colleagues hypothesize that the ankyrin repeats can undergo a large conformational change (Salazar et al. 2008). Although there is little precedent for such large conformational changes in ankyrin repeats (Gaudet, 2008), there is evidence that some ankyrin repeats are unstable and only fold stably in the presence of ligands (Croy et al. 2004). The first ankyrin repeat of the TRPV1 has high B-factors – a crystallographic parameter that indicates the degree of disorder of a protein region, and by inference, can hint at its stability. This suggests that the first ankyrin repeat may be unstable under some circumstances, which could reconcile the apparent discrepancies between the structural information on TRPV1 and the function assigned to C157. This hypothesis is readily testable, providing a basis for further experiments on the molecular mechanism of TRPV1 activation by allicin.

TRPM proteins are implicated in a range of physiological processes, including response to oxidative stress (TRPM2), T-cell activation (TRPM4), taste (TRPM5), magnesium homeostasis (TRPM6 and TRPM7) and temperature sensation (TRPM8) (see Harteneck, 2005, for a review). TRPM channels share a coiled-coil domain located C-terminal of the transmembrane domain (Jenke et al. 2003; Montell, 2005). When expressed by themselves, these coiled-coil domains formed tetramers, as observed using a range of biophysical techniques (Tsuruda et al. 2006). While these domains have so far resisted high-resolution structure determination, the biophysical observations can be used to generate molecular models of these homotetramers to inform functional studies on TRPM channels. For example, engineered or natural mutations in the coiled-coil region can be assigned as either exposed to solvent (and potential ligands) or buried and participating in tetramerization. This assignment could then be directly tested by studying the mutant versions of the coiled-coil domain using the same biophysical techniques. Recent reports on the coiled-coil domain of TRPM8, TRPM2 and TRPM4 have shown that the coiled coil is important for TRPM protein integrity and function (Launay et al. 2004; Erler et al. 2006; Mei et al. 2006). A TRPM8 deletion construct missing the C-terminal coiled coil still tetramerizes and traffics to the plasma membrane when expressed heterologously in insect cells, but the coiled coil is necessary for channel activation (Phelps & Gaudet, 2007). In aggregate, studies of the TRPM channel coiled coils indicate that they have two important roles: (i) the coiled coil helps direct subunit assembly into functional channels; and (ii) it is also necessary for channel activation, at least in the response of TRPM8 to cool-tasting ligands or cool temperatures.

Two TRPM channels important in magnesium homeostasis, TRPM6 and TRPM7, have an unusual kinase domain – termed α-kinase – appended to their C-terminus (Nadler et al. 2001; Riazanova et al. 2001; Runnels et al. 2001). The structure of the isolated TRPM7 α-kinase domain, determined by X-ray crystallography, revealed a domain-swapped dimer: the N-terminal α-helix of one subunit interacts with the second subunit and vice versa (Fig. 2D; Yamaguchi et al. 2001). In a tetrameric TRPM7 channel, there would therefore be two such dimers. The structure represents the first example of an α-kinase, and revealed significant structural homology to other kinases, with N- and C-lobes that sandwich the nucleotide substrate (Fig. 2D). A conserved glycine-rich loop, which had been hypothesized to interact with the triphosphate moiety of ATP, is instead poised to interact with the peptide substrate (Yamaguchi et al. 2001). Furthermore, a zinc-binding site appears to play a structural role, stabilizing the C-lobe. Follow-up studies used the structure to generate mutant channels with impaired kinase activity and examine the role of the kinase domain in TRPM7 function. On one hand, kinase activity was not required for modulation of TRPM7 activity through internal magnesium concentrations (Matsushita et al. 2005). On the other hand, the kinase activity is necessary for modulation through G-protein- and protein kinase A-dependent cyclic AMP levels (Takezawa et al. 2004).

The examples above highlight how structures of isolated protein domains can provide information on TRP channel function. There are still many TRP channel domains to which this divide-and-conquer strategy could be applied productively. For example, TRPM2 is regulated by ADP-ribose and related compounds through its NUDIX domain (Eisfeld & Lückhoff, 2007). Structural and biochemical studies of the isolated NUDIX domain could help resolve molecular mechanisms of these regulatory processes. TRPA1, a pain receptor and sensor of pungent compounds, is activated through the modification of cysteines in the ankyrin repeat region (Hinman et al. 2006; Macpherson et al. 2007), and structures of the modified and unmodified ankyrin repeats could shed light on the activation mechanism. Moreover, both the structure and function of the MHRs of TRPM channels remain rather enigmatic, although mutations in the MHRs of TRPM6 channel are associated with hypomagnesaemia (Schlingmann et al. 2007) and deletions impair trafficking of TRPM2, TRPM4 and TRPM8 channels (Perraud et al. 2003; Launay et al. 2004; Phelps & Gaudet, 2007). Structural information could also help elucidate the role of the extracellular loops of TRPML and TRPP channels. Finally, many protein partners have been identified that interact with and regulate TRP channels. Structures of isolated TRP channel domains in complex with these binding partners will be important in mapping out the structural basis of TRP channel regulation. In summary, although the divide-and-conquer approach of seeking structural information on isolated domains of TRP channels has already reaped some results, it is likely that we have only seen the tip of the iceberg.

Blurry images? Single-particle electron microscopy of TRP channels

Electron microscopy (EM) can be a powerful technique to study large macromolecules and complexes (see Henderson, 2004; Chiu et al. 2005; Jiang & Ludtke, 2005, for recent reviews of the technique), and has proven quite useful in the study of membrane protein structure. In single particle EM, images of thousands of single macromolecules are classified according to the orientation of the macromolecules, and then used to reconstruct the three-dimensional structure of the macromolecule. Single particle EM reconstructions typically range in resolution from 20 to 30 Å for negative stain samples – where the sample is embedded in a thin layer of a heavy metal salt like uranyl acetate to enhance constrast, after adhering to a carbon-coated EM grid – to near-atomic resolution for some cryo-EM studies. Thus far only cases with a high degree of symmetry have yielded the near-atomic resolution necessary to trace the path of the protein chain with certainty, because the symmetry averaging enhances the signal to noise of the images. Examples of cryo-EM structures with near-atomic resolution include the 7-fold symmetric chaperone GroEL (Ludtke et al. 2008), the rotavirus inner capsid particle using 13-fold averaging (Zhang et al. 2008), and the infectious epsilon15 particle with icosahedral symmetry (Jiang et al. 2008). With current methodologies, resolution values often range from 10 to 20 Å for cryo-EM structures, where the samples are imaged after freezing in a thin layer of vitrified ice, although cryo-EM structures now regularly provide reconstructions at better than 10 Å resolution. Of note, although they share the same units, these resolution values do not correspond to the numbers quoted for X-ray crystal structures since the two techniques use different data and statistics, and therefore should not be directly compared. For reference, in cryo-EM, resolution in the range of 6–9 Å is required to visualize secondary structure elements like rods corresponding to α-helices, ~4 Å to resolve individual strands of a β-sheet, or ~8 Å to see the grooves in RNA.

Thus far, one representative for each of the three central families of TRP channels has been imaged by single particle EM (Fig. 3AC and G): TRPC3 (Mio et al. 2007), TRPM2 (Maruyama et al. 2007) and TRPV1 (Moiseenkova-Bell et al. 2008). The TRPC3 structure was determined by the Sato group using cryo-EM at a stated resolution of 15 Å using protein purified from transiently transfected HEK293 cells (Mio et al. 2007). The resulting structure, measuring 240 × 200 × 200 Å3, is surprisingly large for a 388 kDa tetramer. For comparison, the crystal structure of the 228 kDa Shaker channel is approximately 135 × 95 × 95 Å3 (Long et al. 2005) and a compact globular 30 kDa protein domain would fit in a ~33 × 33 × 33 Å3 cube or a 40 Å diameter sphere. The TRPC3 EM reconstruction is also mesh-like, and the density in the region assigned as transmembrane is rather different than the structure of the Shaker channel with which it shares sequence homology (compare Fig. 3A and 3D). The TRPM2 structure was determined by the same group to a stated resolution of 28 Å using three-dimensional reconstruction from negative-stained samples also isolated from transfected HEK293 cells (Maruyama et al. 2007). Of note, different resolution criteria were used for TRPC3 and TRPM2; if the more stringent criterion applied to the TRPC3 structure (and the TRPV1 structure described below) is also applied to TRPM2, the corresponding resolution is 37 Å. The bell-shaped TRPM2 structure is also very large at 250 × 170 × 170 Å3, although its 689 kDa tetrameric molecular mass is significantly larger than TRPC3. The TRPM2 structure is surprisingly featureless for a protein complex of that size (Fig. 3B), suggesting that some of the features may have been averaged out during the particle alignment and/or symmetry averaging of the particles, which could happen with a sample that has multiple heterogeneous conformations. There is also no obvious structural feature resembling the NUDIX domain, for which a homologous crystal structure is available (compare Fig. 3B and F). The most recent addition to the collection of TRP channel EM reconstructions is a cryo-EM structure of the ~400 kDa TRPV1 tetramer purified from a heterologous S. cerevisiae expression system (Moiseenkova-Bell et al. 2008). The native state of the purified TRPV1 protein was validated by measuring calcium flow in response to resiniferatoxin after reconstitution into proteoliposomes. The resulting 19 Å structure is ~150 × 100 × 100 Å3 and the particles had clear signs of the expected 4-fold symmetry before averaging was applied. The TRPV1 shape is likened to a ‘hanging gondola’ (Fig. 3C), a term that was coined for the similarly shaped EM reconstructions of voltage-gated channels (Kobertz et al. 2000; Kim et al. 2004).

Figure 3
Electron microscopy of TRP channels

What can be learned from these low-resolution structures of complete TRP channels? In the case of the TRPV1 structure, the crystal structures of the transmembrane domain of the Shaker channel (Fig. 3D; Long et al. 2005, 2007) and the TRPV1 ankyrin repeat domain (Fig. 3E; Lishko et al. 2007) could be placed within the model with reasonable confidence, providing a hypothesis of their relative position within the intact protein (Moiseenkova-Bell et al. 2008). The TRPV1 density assigned to the N-terminal ankyrin repeats is part of a continuous and hollow bowl-shaped density that forms the cabin of the ‘gondola’ (Fig. 3C and E). Together with the fact that the isolated TRPV1 ankyrin repeats do not oligomerize (Lishko et al. 2007), a testable hypothesis emerges that the N- and C-terminal cytosolic segments of TRPV1 are in contact with each other, forming an intricate quaternary structure. In this context, it is interesting to consider a study showing that the swapping of the C-terminal domains of TRPM8 (containing the coiled coil discussed above) and TRPV1 (which has no predicted coiled coil) results in the swapping of their thermosensing properties (Brauchi et al. 2006). A chimera containing the TRPV1 N-terminal and transmembrane domains with the C-terminal domain of TRPM8 was sensitive to capsaicin and cold, whereas a chimera of the TRPM8 N-terminal and transmembrane domains with the C-terminal domain of TRPV1 was sensitive to menthol and heat (Brauchi et al. 2006). If the N- and C-terminal domains of TRPV1 interact in the native TRPV1 channel as hypothesized above, it is surprising that the TRPM8 C-terminal domain can effectively replace that of TRPV1. Further studies are required to determine the impact of the chimeras on channel structure.

Unless structures of homologous proteins or fragments are available, it is difficult to assign particular protein domains to distinct densities of low-resolution EM reconstructions. One approach is to combine the reconstruction with electron microscopy imaging of complexes of the macromolecule under study with antibodies. By locating the antibody binding site on the reconstructed model, the relevant density can then be assigned to a particular protein fragment. This approach was used in the three-dimensional reconstructions of TRPC3 and TRPM2 (Maruyama et al. 2007; Mio et al. 2007), although the information obtained thus far is somewhat limited because only antibodies to C-terminal tags were used.

Clearly, we are still in the early days of electron microscopy of TRP channels. There are several previous examples of membrane protein families where the early reconstructions from different laboratories were difficult to reconcile with each other, including ABC transporters (some of the early work includes Rosenberg et al. 1997, 2001; Velarde et al. 2001; Chami et al. 2002), inositol 1,4,5-trisphosphate (IP3) receptors (much of the EM work is reviewed in Bosanac et al. 2004), and AMPA ionotropic glutamate receptors (Tichelaar et al. 2004; Nakagawa et al. 2005). The bottom line is that these ‘blurry images’, or low-resolution views generated by single-particle EM reconstructions, should be considered working models that can and should be tested by further experiments – using functional, biochemical, biophysical and structural approaches. Just like any model, they should not be used at the exclusion of others, until they are supported by coherent datasets from multiple approaches and/or reach a near-atomic resolution that leaves little doubt about the details of the protein's fold, intramolecular packing and domain organization.


Determining the three-dimensional structures of TRP channel cytosolic domains alone and in complex with small molecule or protein ligands is an important step in understanding the molecular basis of channel gating and regulatory mechanisms. The cytosolic domains serve as important regulatory modules sensing specific aspects of the cell state, such as the intracellular concentration of signalling molecules or metabolites. Structural information obtained on the cytosolic domains of TRP channels can be combined with data from biochemical and electrophysiology assays to define the molecular mechanisms of channel modulation by intracellular signals. An inherent problem with this divide-and-conquer approach is that it provides few insights into how the domains communicate within the context of the whole channel. Electron microscopy studies of TRP channels, although still in their early days, are generating some views of entire TRP channels that can provide a context for much of the accumulated structural and functional data. Ultimately, the Holy Grail of TRP channel structural biology is high-resolution structures of entire TRP channels. Although membrane protein structure determination remains a challenge, the relevant methods are improving (see Lacapere et al. 2007, for a recent review). Advances in cryo-EM and membrane protein production methodologies could lead to significant improvements in the resolution of three-dimensional reconstructions of TRP channels. X-ray crystallography is another obvious approach that is actively being explored by many labs interested in TRP channels. The progress in the field, illustrated by recent structures of eukaryotic potassium channels (Long et al. 2005, 2007) and acid-sensing ion channel (ASIC; Jasti et al. 2007), provides encouraging signs that such endeavours are feasible. Furthermore, membrane proteins can readily be restricted to a two-dimensional space when embedded in a lipid bilayer, making them excellent candidates for electron crystallography of two-dimensional crystals, a technique that is seeing a revival (Hite et al. 2007; Ford & Holzenburg, 2008).

The interest in structural biology of TRP channels also prompts the question: What are some of the important physiological functions of TRP channels that can be better understood through structural approaches? First, some TRP channels are gated by temperature (Tominaga & Caterina, 2004; Latorre et al. 2007) and others may be gated by mechanical stimuli (Christensen & Corey, 2007). Understanding the molecular basis of sensing and responding to these physical stimuli by a conformational change will require both detailed structural models of the relevant TRP channels as well as biophysical experiments interpreted in light of the structural information. Second, TRP channel structures, perhaps in the presence of phosphoinositides, will help us understand whether there is a common mechanism for the regulation of many TRP channels by phosphoinositides. Similarly, many TRP channels are regulated by calcium and calmodulin (Zhu, 2005). Is the structural basis of this regulation the same? Functional data suggest that it differs, and structural analyses of complexes of TRP channels and/or isolated domains with calmodulin will provide additional insights. Third, high-resolution structures of TRP channel pores will help elucidate the structural basis for their selectivity, which varies from TRP channel to TRP channel, with some like TRPV5 and TRPV6 being highly calcium selective, others like TRPM4 and TRPM5, highly monovalent selective, and yet others like TRPV1–4 and many TRPCs, non-selective cation channels (see Venkatachalam & Montell, 2007, for a recent review). Finally, many TRP channels have been implicated in pain, inflammation and disease, either directly through TRP channel mutations or indirectly by contributing to the symptoms and progression of disease (Nilius et al. 2007). This makes TRP channels important targets in the development of new drugs and therapeutic approaches. All high-resolution TRP channel structures can contribute to the development of new pharmaceutics through structure-based drug design. In summary, TRP channels have definitely entered the structural era and we can anticipate many more mechanistic insights from TRP channel structural biology.


I thank Andres Leschziner and members of my laboratory, particularly Albert Li and Christopher Phelps, for helpful discussions and Moiseenkova-Bell et al. for sharing their manuscript prior to publication. This work was funded by a Klingenstein Fellowship Award in the Neurosciences and NIH 1R01GM081340 to R.G.


  • Barrera NP, Shaifta Y, McFadzean I, Ward JPT, Henderson RM, Edwardson JM. AFM imaging reveals the tetrameric structure of the TRPC1 channel. Biochem Biophys Res Comms. 2007;358:1086–1090. [PubMed]
  • Bosanac I, Michikawa T, Mikoshiba K, Ikura M. Structural insights into the regulatory mechanism of IP3 receptor. Biochim Biophys Acta. 2004;1742:89–102. [PubMed]
  • Brauchi S, Orta G, Salazar M, Rosenmann E, Latorre R. A hot-sensing cold receptor: C-terminal domain determines thermosensation in transient receptor potential channels. J Neurosci. 2006;26:4835–4840. [PubMed]
  • Cahalan MD. Cell biology. Channels as enzymes. Nature. 2001;411:542–543. [PubMed]
  • Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D. The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature. 1997;389:816–824. [PubMed]
  • Chami M, Steinfels E, Orelle C, Jault JM, Di Pietro A, Rigaud JL, Marco S. Three-dimensional structure by cryoelectron microscopy of YvcC, an homodimeric ATP-binding cassette transporter from Bacillus subtilis. J Mol Biol. 2002;315:1075–1085. [PubMed]
  • Chiu W, Baker ML, Jiang W, Dougherty M, Schmid MF. Electron cryomicroscopy of biological machines at subnanometer resolution. Structure. 2005;13:363–372. [PubMed]
  • Christensen AP, Corey DP. TRP channels in mechanosensation: direct or indirect activation? Nat Rev Neurosci. 2007;8:510–521. [PubMed]
  • Clapham DE. TRP channels as cellular sensors. Nature. 2003;426:517–524. [PubMed]
  • Croy CH, Bergqvist S, Huxford T, Ghosh G, Komives EA. Biophysical characterization of the free IκBα ankyrin repeat domain in solution. Protein Sci. 2004;13:1767–1777. [PMC free article] [PubMed]
  • Cuajungco MP, Grimm C, Oshima K, D'Hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S. PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4. J Biol Chem. 2006;281:18753–18762. [PubMed]
  • D'Hoedt D, Owsianik G, Prenen J, Cuajungco MP, Grimm C, Heller S, Voets T, Nilius B. Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3. J Biol Chem. 2008;283:6272–6280. [PubMed]
  • Doyle DA, Morais Cabral J, Pfuetzner RA, Kuo A, Gulbis JM, Cohen SL, Chait BT, MacKinnon R. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science. 1998;280:69–77. [PubMed]
  • Eder P, Poteser M, Groschner K. Transient Receptor Potential (TRP) Channels. Vol. 179. Heidelberg: Springer; 2007. TRPC3: a multifunctional, pore-forming signalling molecule; pp. 77–92. [PubMed]
  • Eisfeld J, Lückhoff A. Transient Receptor Potential (TRP) Channels. Vol. 179. Heidelberg: Springer; 2007. TRPM2; pp. 237–252.
  • Erler I, Al-Ansary DM, Wissenbach U, Wagner TF, Flockerzi V, Niemeyer BA. Trafficking and assembly of the cold-sensitive TRPM8 channel. J Biol Chem. 2006;281:38396–38404. [PubMed]
  • Fleig A, Penner R. The TRPM ion channel subfamily: molecular, biophysical and functional features. Trends Pharmacol Sci. 2004;25:633–639. [PubMed]
  • Ford RC, Holzenburg A. Electron crystallography of biomolecules: mysterious membranes and missing cones. Trends Biochem Sci. 2008;33:38–43. [PubMed]
  • Gaudet R. Structural insights into the function of TRP channels. In: Liedtke W, Heller S, editors. TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades. Boca Raton, FL: CRC Press; 2006. pp. 349–359. [PubMed]
  • Gaudet R. A primer on ankyrin repeat function in TRP channels and beyond. Mol Biosyst. 2008;4:372–379. [PMC free article] [PubMed]
  • Grisshammer R. Understanding recombinant expression of membrane proteins. Curr Opin Biotechnol. 2006;17:337–340. [PubMed]
  • Harteneck C. Function and pharmacology of TRPM cation channels. Naunyn Schmiedebergs Arch Pharmacol. 2005;371:307–314. [PubMed]
  • Henderson R. Realizing the potential of electron cryomicroscopy. Q Rev Biophys. 2004;37:3–13. [PubMed]
  • Hinman A, Chuang H-H, Bautista DM, Julius D. TRP channel activation by reversible covalent modification. Proc Natl Acad Sci U S A. 2006;103:19564–19568. [PMC free article] [PubMed]
  • Hite RK, Raunser S, Walz T. Revival of electron crystallography. Curr Opin Struct Biol. 2007;17:389–395. [PMC free article] [PubMed]
  • Jasti J, Furukawa H, Gonzales EB, Gouaux E. Structure of acid-sensing ion channel 1 at 1.9 Å resolution and low pH. Nature. 2007;449:316–323. [PubMed]
  • Jenke M, Sanchez A, Monje F, Stuhmer W, Weseloh RM, Pardo LA. C-terminal domains implicated in the functional surface expression of potassium channels. EMBO J. 2003;22:395–403. [PMC free article] [PubMed]
  • Jiang W, Baker ML, Jakana J, Weigele PR, King J, Chiu W. Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy. Nature. 2008;451:1130–1134. [PubMed]
  • Jiang W, Ludtke SJ. Electron cryomicroscopy of single particles at subnanometer resolution. Curr Opin Struct Biol. 2005;15:571–577. [PubMed]
  • Jin X, Touhey J, Gaudet R. Structure of the N-terminal ankyrin repeat domain of the TRPV2 ion channel. J Biol Chem. 2006;281:25006–25010. [PubMed]
  • Jordt SE, Julius D. Molecular basis for species-specific sensitivity to ‘hot’ chili peppers. Cell. 2002;108:421–430. [PubMed]
  • Jung J, Lee SY, Hwang SW, Cho H, Shin J, Kang YS, Kim S, Oh U. Agonist recognition sites in the cytosolic tails of vanilloid receptor 1. J Biol Chem. 2002;277:44448–44454. [PubMed]
  • Kedei N, Szabo T, Lile JD, Treanor JJ, Olah Z, Iadarola MJ, Blumberg PM. Analysis of the native quaternary structure of vanilloid receptor 1. J Biol Chem. 2001;276:28613–28619. [PubMed]
  • Kim LA, Furst J, Gutierrez D, Butler MH, Xu S, Goldstein SA, Grigorieff N. Three-dimensional structure of ItoKv4.2-KChIP2 ion channels by electron microscopy at 21 Å resolution. Neuron. 2004;41:513–519. [PubMed]
  • Kobertz WR, Williams C, Miller C. Hanging gondola structure of the T1 domain in a voltage-gated K+ channel. Biochemistry. 2000;39:10347–10352. [PubMed]
  • Kwon Y, Hofmann T, Montell C. Integration of phosphoinositide- and calmodulin-mediated regulation of TRPC6. Mol Cell. 2007;25:491–503. [PMC free article] [PubMed]
  • Lacapere JJ, Pebay-Peyroula E, Neumann JM, Etchebest C. Determining membrane protein structures: still a challenge! Trends Biochem Sci. 2007;32:259–270. [PubMed]
  • Latorre R, Brauchi S, Orta G, Zaelzer C, Vargas G. ThermoTRP channels as modular proteins with allosteric gating. Cell Calcium. 2007;42:427–438. [PubMed]
  • Launay P, Cheng H, Srivatsan S, Penner R, Fleig A, Kinet JP. TRPM4 regulates calcium oscillations after T cell activation. Science. 2004;306:1374–1377. [PubMed]
  • Lishko PV, Procko E, Jin X, Phelps CB, Gaudet R. The ankyrin repeats of TRPV1 bind multiple ligands and modulate channel sensitivity. Neuron. 2007;54:905–918. [PubMed]
  • Long SB, Campbell EB, Mackinnon R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science. 2005;309:897–903. [PubMed]
  • Long SB, Tao X, Campbell EB, MacKinnon R. Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment. Nature. 2007;450:376–382. [PubMed]
  • Ludtke SJ, Baker ML, Chen DH, Song JL, Chuang DT, Chiu W. De novo backbone trace of GroEL from single particle electron cryomicroscopy. Structure. 2008;16:441–448. [PubMed]
  • McCleverty CJ, Koesema E, Patapoutian A, Lesley SA, Kreusch A. Crystal structure of the human TRPV2 channel ankyrin repeat domain. Protein Sci. 2006;15:2201–2206. [PMC free article] [PubMed]
  • Macpherson LJ, Dubin AE, Evans MJ, Marr F, Schultz PG, Cravatt BF, Patapoutian A. Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature. 2007;445:541–545. [PubMed]
  • Maruyama Y, Ogura T, Mio K, Kiyonaka S, Kato K, Mori Y, Sato C. Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, bell-shaped structure of transient receptor potential melastatin type 2 cation channel. J Biol Chem. 2007;282:36961–36970. [PubMed]
  • Matsushita M, Kozak JA, Shimizu Y, McLachlin DT, Yamaguchi H, Wei F-Y, Tomizawa K, Matsui H, Chait BT, Cahalan MD, Nairn AC. Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1. J Biol Chem. 2005;280:20793–20803. [PubMed]
  • Mei ZZ, Xia R, Beech DJ, Jiang LH. Intracellular coiled-coil domain engaged in subunit interaction and assembly of melastatin-related transient receptor potential channel 2. J Biol Chem. 2006;281:38748–38756. [PMC free article] [PubMed]
  • Minor DL., Jr The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data. Neuron. 2007;54:511–533. [PMC free article] [PubMed]
  • Mio K, Ogura T, Kiyonaka S, Hiroaki Y, Tanimura Y, Fujiyoshi Y, Mori Y, Sato C. The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas. J Mol Biol. 2007;367:373–383. [PubMed]
  • Moiseenkova-Bell VY, Stanciu LA, Serysheva II, Tobe BJ, Wensel TG. Structure of TRPV1 channel revealed by electron cryomicroscopy. Proc Natl Acad Sci U S A. 2008;105:7451–7455. [PMC free article] [PubMed]
  • Montell C. The TRP superfamily of cation channels. Sci STKE 2005. 2005:re3. [PubMed]
  • Mosavi LK, Cammett TJ, Desrosiers DC, Peng ZY. The ankyrin repeat as molecular architecture for protein recognition. Protein Sci. 2004;13:1435–1448. [PMC free article] [PubMed]
  • Musacchio A. How SH3 domains recognize proline. In: Janin J, Shoshana JW, editors. Advances in Protein Chemistry. New York: Academic Press; 2002. pp. 211–268. [PubMed]
  • Myers BR, Julius D. TRP channel structural biology: new roles for an old fold. Neuron. 2007;54:847–850. [PubMed]
  • Nadler MJ, Hermosura MC, Inabe K, Perraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet JP, Penner R, Scharenberg AM, Fleig A. LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability. Nature. 2001;411:590–595. [PubMed]
  • Nakagawa T, Cheng Y, Ramm E, Sheng M, Walz T. Structure and different conformational states of native AMPA receptor complexes. Nature. 2005;433:545–549. [PubMed]
  • Nazýroðlu M. New molecular mechanisms on the activation of TRPM2 channels by oxidative stress and ADP-ribose. Neurochem Res. 2007;32:1990–2001. [PubMed]
  • Niemeyer BA. Structure-function analysis of TRPV channels. Naunyn Schmiedebergs Arch Pharmacol. 2005;371:285–294. [PubMed]
  • Nilius B, Owsianik G, Voets T, Peters JA. Transient receptor potential cation channels in disease. Physiol Rev. 2007;87:165–217. [PubMed]
  • Numazaki M, Tominaga T, Takeuchi K, Murayama N, Toyooka H, Tominaga M. Structural determinant of TRPV1 desensitization interacts with calmodulin. Proc Natl Acad Sci U S A. 2003;100:8002–8006. [PMC free article] [PubMed]
  • O'Neil RG, Brown RC. The vanilloid receptor family of calcium-permeable channels: molecular integrators of microenvironmental stimuli. News Physiol Sci. 2003;18:226–231. [PubMed]
  • Perraud AL, Fleig A, Dunn CA, Bagley LA, Launay P, Schmitz C, Stokes AJ, Zhu Q, Bessman MJ, Penner R, Kinet JP, Scharenberg AM. ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology. Nature. 2001;411:595–599. [PubMed]
  • Perraud AL, Schmitz C, Scharenberg AM. TRPM2 Ca2+ permeable cation channels: from gene to biological function. Cell Calcium. 2003;33:519–531. [PubMed]
  • Phelps CB, Gaudet R. The role of the N terminus and transmembrane domain of TRPM8 in channel localization and tetramerization. J Biol Chem. 2007;282:36474–36480. [PubMed]
  • Phelps CB, Huang RJ, Wang RR, Gaudet R. Structural analyses of the ankyrin repeat domain of TRPV6 and related TRPV ion channels. Biochemistry. 2008;47:2476–2484. [PMC free article] [PubMed]
  • Phelps CB, Procko E, Lishko PV, Wang RR, Gaudet R. Insights into the roles of conserved and divergent residues in the ankyrin repeats of TRPV ion channels. Channels. 2007;1:148–151. [PubMed]
  • Ramsey IS, Delling M, Clapham DE. An introduction to TRP channels. Annu Rev Physiol. 2006;68:619–647. [PubMed]
  • Riazanova LV, Pavur KS, Petrov AN, Dorovkov MV, Riazanov AG. [Novel type of signaling molecules: protein kinases covalently linked to ion channels] Mol Biol (Mosk) 2001;35:321–332. [PubMed]
  • Rosenbaum T, Gordon-Shaag A, Munari M, Gordon SE. Ca2+/calmodulin modulates TRPV1 activation by capsaicin. J Gen Physiol. 2004;123:53–62. [PMC free article] [PubMed]
  • Rosenberg MF, Callaghan R, Ford RC, Higgins CF. Structure of the multidrug resistance P-glycoprotein to 2.5 nm resolution determined by electron microscopy and image analysis. J Biol Chem. 1997;272:10685–10694. [PubMed]
  • Rosenberg MF, Mao Q, Holzenburg A, Ford RC, Deeley RG, Cole SP. The structure of the multidrug resistance protein 1 (MRP1/ABCC1). crystallization and single-particle analysis. J Biol Chem. 2001;276:16076–16082. [PubMed]
  • Runnels LW, Yue L, Clapham DE. TRP-PLIK, a bifunctional protein with kinase and ion channel activities. Science. 2001;291:1043–1047. [PubMed]
  • Salazar H, Llorente I, Jara-Oseguera A, Garcia-Villegas R, Munari M, Gordon SE, Islas LD, Rosenbaum T. A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. Nat Neurosci. 2008;11:255–261. [PubMed]
  • Schlingmann KP, Waldegger S, Konrad M, Chubanov V, Gudermann T. TRPM6 and TRPM7 – gatekeepers of human magnesium metabolism. Biochim Biophys Acta. 2007;1772:813–821. [PubMed]
  • Soboloff J, Spassova M, Hewavitharana T, He LP, Luncsford P, Xu W, Venkatachalam K, van Rossum D, Patterson RL, Gill DL. TRPC channels: integrators of multiple cellular signals. Handb Exp Pharmacol. 2007:575–591. [PubMed]
  • Stein AT, Ufret-Vincenty CA, Hua L, Santana LF, Gordon SE. Phosphoinositide 3-kinase binds to TRPV1 and mediates NGF-stimulated TRPV1 trafficking to the plasma membrane. J Gen Physiol. 2006;128:509–522. [PMC free article] [PubMed]
  • Takezawa R, Schmitz C, Demeuse P, Scharenberg AM, Penner R, Fleig A. Receptor-mediated regulation of the TRPM7 channel through its endogenous protein kinase domain. Proc Natl Acad Sci U S A. 2004;101:6009–6014. [PMC free article] [PubMed]
  • Tichelaar W, Safferling M, Keinanen K, Stark H, Madden DR. The three-dimensional structure of an ionotropic glutamate receptor reveals a dimer-of-dimers assembly. J Mol Biol. 2004;344:435–442. [PubMed]
  • Tominaga M, Caterina MJ. Thermosensation and pain. J Neurobiol. 2004;61:3–12. [PubMed]
  • Tsuruda PR, Julius D, Minor DL., Jr Coiled coils direct assembly of a cold-activated TRP channel. Neuron. 2006;51:201–212. [PMC free article] [PubMed]
  • Velarde G, Ford RC, Rosenberg MF, Powis SJ. Three-dimensional structure of transporter associated with antigen processing (TAP) obtained by single particle image analysis. J Biol Chem. 2001;276:46054–46063. [PubMed]
  • Venkatachalam K, Montell C. TRP Channels. Annu Rev Biochem. 2007;76:387–417. [PubMed]
  • Voets T, Janssens A, Droogmans G, Nilius B. Outer pore architecture of a Ca2+-selective TRP channel. J Biol Chem. 2004;279:15223–15230. [PubMed]
  • Yamaguchi H, Matsushita M, Nairn AC, Kuriyan J. Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity. Mol Cell. 2001;7:1047–1057. [PubMed]
  • Zhang X, Settembre E, Xu C, Dormitzer PR, Bellamy R, Harrison SC, Grigorieff N. Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction. Proc Natl Acad Sci U S A. 2008;105:1867–1872. [PMC free article] [PubMed]
  • Zhu MX. Multiple roles of calmodulin and other Ca2+-binding proteins in the functional regulation of TRP channels. Pflugers Arch. 2005;451:105–115. [PubMed]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society
PubReader format: click here to try


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...