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J Bacteriol. May 1968; 95(5): 1627–1633.
PMCID: PMC252187

Cell Division During Inhibition of Deoxyribonucleic Acid Synthesis in Escherichia coli

Abstract

When cultures of Escherichia coli B/r growing at various rates were exposed to ultraviolet light, mitomycin C, or nalidixic acid, deoxyribonucleic acid (DNA) synthesis stopped but cell division continued for at least 20 min. The chromosome configurations in the cells which divided were estimated by determining the rate of DNA synthesis during the division cycle. The cultures were pulse-labeled with 14C-thymidine, and the amount of label incorporated into cells of different ages was found by measuring the radioactivity in cells born subsequent to the labeling period. The cells which divided in the absence of DNA synthesis were those which had completed a round of chromosome replication prior to the treatments. It was concluded that completion of a round of replication is a necessary and sufficient condition of DNA synthesis for cell division.

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Selected References

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  • Cooper S, Helmstetter CE. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. [PubMed]
  • Deitz WH, Cook TM, Goss WA. Mechanism of action of nalidixic acid on Escherichia coli. 3. Conditions required for lethality. J Bacteriol. 1966 Feb;91(2):768–773. [PMC free article] [PubMed]
  • GOSS WA, DEITZ WH, COOK TM. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:1112–1118. [PMC free article] [PubMed]
  • GOSS WA, DEITZ WH, COOK TM. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965 Apr;89:1068–1074. [PMC free article] [PubMed]
  • Helmstetter CE. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. [PubMed]
  • HELMSTETTER CE, CUMMINGS DJ. AN IMPROVED METHOD FOR THE SELECTION OF BACTERIAL CELLS AT DIVISION. Biochim Biophys Acta. 1964 Mar 16;82:608–610. [PubMed]
  • KELNER A. Growth, respiration, and nucleic acid synthesis in ultraviolet-irradiated and in photoreactivated Escherichia coli. J Bacteriol. 1953 Mar;65(3):252–262. [PMC free article] [PubMed]
  • KERSTEN H. Action of mitomycin C on nucleic acid metabolism in tumor and bacterial cells. Biochim Biophys Acta. 1962 Apr 2;55:558–560. [PubMed]
  • Koch AL. On the difference between the lethal effects of H3 and P32 in bacteria. Radiat Res. 1966 Sep;29(1):18–32. [PubMed]
  • LARK KG, MAALOE O, ROSTOCK O. Cytological studies of nuclear division in Salmonella typhimurium. J Gen Microbiol. 1955 Oct;13(2):318–326. [PubMed]
  • Mazia D, Zeuthen E. Blockage and delay of cell division in synchronized populations of Tetrahymena by mercaptoethanol (monothioethylene glycol). C R Trav Lab Carlsberg. 1966;35(14):341–361. [PubMed]
  • Nachtwey DS. Division of synchronized Tetrahymena pyriformis after emacronucleation. C R Trav Lab Carlsberg. 1965;35(2):25–35. [PubMed]
  • Phillips SL, Person S, Jagger J. Division delay induced in Escherichia coli by near-ultraviolet radiation. J Bacteriol. 1967 Jul;94(1):165–170. [PMC free article] [PubMed]
  • Rasmussen L. Effect of DL-p-fluorophenylalanine on Paramecium aurelia during the cell generation cycle. Exp Cell Res. 1967 Feb;45(2):501–504. [PubMed]
  • REICH E, SHATKIN AJ, TATUM EL. Bacteriocidal action of mitomycin C. Biochim Biophys Acta. 1961 Oct 14;53:132–149. [PubMed]
  • SACHSENMAIER W, RUSCH HP. THE EFFECT OF 5-FLUORO-2'-DEOXYURIDINE ON SYNCHRONOUS MITOSIS IN PHYSARUM POLYCEPHALUM. Exp Cell Res. 1964 Oct;36:124–133. [PubMed]
  • SHIBA S, TERAWAKI A, TAGUCHI T, KAWAMATA J. Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by mitomycin C. Nature. 1959 Apr 11;183(4667):1056–1057. [PubMed]
  • Smith DH, Davis BD. Mode of action of novobiocin in Escherichia coli. J Bacteriol. 1967 Jan;93(1):71–79. [PMC free article] [PubMed]
  • Smith-Kielland I. The effect of mitomycin c on deoxyribonucleic acid and messenger ribonucleic acid in Escherichia coli. Biochim Biophys Acta. 1966 Feb 21;114(2):254–263. [PubMed]
  • Smith-Kielland I. DNA turnover in a strain of Escherichia coli treated with mitomycin C. Biochim Biophys Acta. 1967 May 30;138(3):542–549. [PubMed]
  • Suzuki H, Kilgore WW. Effects of mitomycin C on macromolecular synthesis in Escherichia coli. J Bacteriol. 1967 Feb;93(2):675–682. [PMC free article] [PubMed]
  • Swenson PA, Setlow RB. Effects of ultraviolet radiation on macromolecular synthesis in Escherichia coli. J Mol Biol. 1966 Jan;15(1):201–219. [PubMed]
  • Tobey RA, Petersen DF, Anderson EC, Puck TT. Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. Biophys J. 1966 Sep;6(5):567–581. [PMC free article] [PubMed]
  • Williams NE, Zeuthen E. The development of oral fibers in relation to oral morphogenesis and induced division synchrony in Tetrahymena. C R Trav Lab Carlsberg. 1966;35(6):101–118. [PubMed]

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