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J Bacteriol. Jan 1973; 113(1): 263–270.
PMCID: PMC251626

Effect of Reversible Inhibition of Deoxyribonucleic Acid Synthesis on the Yeast Cell Cycle

Abstract

Hydroxyurea (HU) preferentially inhibited deoxyribonucleic acid (DNA) replication and division in Saccharomyces cerevisiae. Growth, ribonucleic acid synthesis, and protein synthesis were less sensitive to this drug. Upon addition of HU, cells underwent one cycle of budding and the nuclei migrated into the necks between the mother cells and buds. Neither the nucleus nor the cells divided. Removal of HU allowed immediate resumption of DNA synthesis. Nuclear division, budding, and cell division occurred 1.5, 2, and 4 hr, respectively, after HU was removed. If protein synthesis was blocked at the time HU was removed, budding and cell division did not occur. These results were interpreted to indicate that HU prevents accumulation of the potential to initiate a new cell cycle.

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Selected References

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  • Culotti J, Hartwell LH. Genetic control of the cell division cycle in yeast. 3. Seven genes controlling nuclear division. Exp Cell Res. 1971 Aug;67(2):389–401. [PubMed]
  • Elford HL. Effect of hydroxyurea on ribonucleotide reductase. Biochem Biophys Res Commun. 1968 Oct 10;33(1):129–135. [PubMed]
  • Grivell AR, Jackson JF. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. J Gen Microbiol. 1968 Dec;54(2):307–317. [PubMed]
  • Hartwell LH. Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967 May;93(5):1662–1670. [PMC free article] [PubMed]
  • Hartwell LH. Biochemical genetics of yeast. Annu Rev Genet. 1970;4:373–396. [PubMed]
  • Hartwell LH. Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol. 1971 Jul 14;59(1):183–194. [PubMed]
  • Hartwell LH, Culotti J, Reid B. Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A. 1970 Jun;66(2):352–359. [PMC free article] [PubMed]
  • Wanka F, Moors J, Krijzer FN. Dissociation of nuclear DNA replication from concomitant protein synthesis in synchronous cultures of Chlorella. Biochim Biophys Acta. 1972 Apr 26;269(1):153–161. [PubMed]
  • PUCK TT, STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. [PMC free article] [PubMed]
  • Robinow CF, Marak J. A fiber apparatus in the nucleus of the yeast cell. J Cell Biol. 1966 Apr;29(1):129–151. [PMC free article] [PubMed]
  • ROSENKRANZ HS, LEVY JA. HYDROXYUREA: A SPECIFIC INHIBITOR OF DEOXYRIBONUCLEIC ACID SYNTHESIS. Biochim Biophys Acta. 1965 Jan 11;95:181–183. [PubMed]
  • Sinclair WK. Hydroxyurea: differential lethal effects on cultured mammalian cells during the cell cycle. Science. 1965 Dec 24;150(3704):1729–1731. [PubMed]
  • Tauro P, Halvorson HO. Effect of gene position on the timing of enzyme synthesis in synchronous cultures of yeast. J Bacteriol. 1966 Sep;92(3):652–661. [PMC free article] [PubMed]
  • Tauro P, Halvorson HO, Epstein RL. Time of gene expression in relation to centromere distance during the cell cycle of Saccharomyces cereviseae. Proc Natl Acad Sci U S A. 1968 Jan;59(1):277–284. [PMC free article] [PubMed]
  • Williamson DH. The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1965 Jun;25(3):517–528. [PMC free article] [PubMed]
  • Vazquez D, Battaner E, Neth R, Heller G, Monro RE. The function of 80 S ribosomal subunits and effects of some antibiotics. Cold Spring Harb Symp Quant Biol. 1969;34:369–375. [PubMed]

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