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Diabetologia
Diabetologia. Sep 2008; 51(9): 1602–1606.
Published online Jul 19, 2008. doi:  10.1007/s00125-008-1095-8
PMCID: PMC2516197

A novel insulin formulation with a more rapid onset of action

Abstract

Aims/hypothesis

This study evaluates the pharmacodynamic and pharmacokinetic properties of the novel ultra-fast insulin product VIAject, a formulation of human soluble insulin and generally recognised as safe ingredients designed to increase the rate of absorption.

Methods

We performed five euglycaemic glucose clamps (Biostator; target blood glucose 5 mmol/l) in ten healthy volunteers. Using a crossover design with a fixed treatment order, 12 IU human soluble insulin, 12 U insulin lispro and 12 IU ultra-fast insulin were injected s.c. in the abdominal region on three study days. On the other two study days, 6 and 3 IU ultra-fast insulin were injected.

Results

Subcutaneous injection of 12 IU ultra-fast insulin resulted in a time–action profile characterised by an even more rapid onset of action and maximal metabolic activity than insulin lispro: time to early half-maximal activity was 33 ± 17 min (mean ± SD) vs insulin lispro 51 ± 13 min vs human soluble insulin 66 ± 15 min (p < 0.05 ultra-fast insulin<insulin lispro<human soluble insulin); time to maximal activity was 136 ± 56 min vs insulin lispro 152 ± 30 min vs human soluble insulin 193 ± 57 min (p < 0.05 ultra-fast insulin and insulin lispro<human soluble insulin). The metabolic activity in the first 2 h after injection was higher with ultra-fast insulin and insulin lispro than with human soluble insulin (AUC glucose infusion rate [GIR] 0–120 min: 915 ± 301 and 781 ± 174 vs 580 ± 164 mg/kg; p < 0.05). A clear dose–response relationship was observed with the three doses of ultra-fast insulin: AUCGIR 0–120 min 12 IU 915 ± 301 vs 6 IU 718 ± 255 vs 3 IU 524 ± 262 mg/kg (p < 0.05). The pharmacokinetic data confirmed the pharmacodynamic results.

Conclusions/interpretation

This study shows that the onset of action of VIAject is faster than that of human soluble insulin and insulin lispro.

Keywords: Insulin therapy, Prandial insulin, Rapid-acting insulin analogues

Introduction

Rapid-acting insulin analogues were developed [1] to meet prandial insulin requirements more effectively. Rather than altering the primary structure of the insulin molecule, a new approach currently under clinical development pulls the zinc ions away from human insulin hexamers and simultaneously masks charges on the surface of the insulin molecule. This causes the insulin hexamers to dissociate and prevents re-association to the hexameric state upon s.c. injection. This is achieved by the addition of EDTA and citric acid. Using this approach, an ultra-fast insulin formulation for s.c. injection was developed (VIAject; Biodel, Danbury, CT, USA) [2]. The aim of this study was to determine the pharmacodynamic and pharmacokinetic properties of this insulin in comparison to the same dose of insulin lispro and human soluble insulin, and to study the dose-responsive properties of this novel insulin at two lower doses.

Methods

We recruited ten healthy volunteers (age 40 [19–61] years, mean [range]; BMI 22.5 [19.2–24.9] kg/m2) to this open-label study that used five euglycaemic glucose clamps (Biostator, mtb Medizintechnik, Ulm, Germany) with blood glucose value 5 mmol/l and basal i.v. insulin infusion 0.15 mU kg−1 min−1 administered at 2 h before until 8 h after glucose dose. The ethics committee of North Rhine in Germany approved this Good Clinical Practice study. Using a crossover study design with a fixed treatment order, 12 IU human soluble insulin (Humulin R; Eli Lilly, Indianapolis, IN, USA), 12 U insulin lispro (Humalog; Eli Lilly) and 12 IU of ultra-fast insulin (VIAject; Biodel) were injected s.c. into the abdominal wall on three study days. On two additional study days 6 and 3 IU of the ultra-fast insulin were studied. This insulin was a re-constituted, lyophilised regular human insulin. In this two-part formulation, the insulin and diluent can be shipped and stored without refrigeration. It is stable at room temperature and for at least two years when refrigerated and frozen. Re-constitution consists of withdrawing 10 ml of diluents with a syringe and injecting it into the vial containing the lyophilised insulin. Reconstitution yields a single, liquid 10 ml vial (25 IU/ml) and in liquid form it should be refrigerated.

Glucose infusion rates (GIR) as determined by the glucose clamp technique were the primary variable for analysis. Sixth-order polynomial functions were fitted to the individual baseline-corrected GIR profiles to determine the following pharmacodynamic summary measures: (1) maximum GIR (GIRmax); (2) time to GIRmax (tGIRmax); (3) time to early/late half-maximal GIRmax (tGIRmax + 50% and tGIRmax  50%); and (4) AUCGIR for specified time intervals.

The secondary variable was the serum insulin concentration profile. From the insulin concentration over time data the following pharmacokinetic variables were derived: (1) maximum insulin concentration (Cmax); (2) time to Cmax (tCmax); (3) time to 50% of Cmax (tCmax+50%); (4) time to 50% of Cmax after Cmax (tCmax−50%); and (5) the AUC of insulin concentrations (AUCINS) for specified time intervals. Human insulin serum concentrations (after administration of human soluble insulin and ultra-fast insulin) were measured by means of a commercial chemoluminescence assay (catalogue number 2-001; MLT-Research, Cardiff, UK). The assay sensitivity was 0.25 μU/ml. Insulin lispro serum concentrations were measured by means of a lispro-specific commercial RIA kit (catalogue number LPI-16K; Linco Research, St Charles, MO, USA). Analyses were performed by IKFE, Mainz, Germany. Pharmacokinetic variables were calculated directly from the assayed values. Since the assay employed for ultra-fast insulin and human soluble insulin measured endogenous insulin and the insulin administered at the glucose clamp procedure, the measured levels were adjusted for baseline and any values less than zero were set to zero. The respective pharmacokinetic variables were calculated from the adjusted data as follows: Cmax and tCmax were reported from the observed adjusted maximum values, the early and late tCmax50% were calculated by linear interpolation between the two closest time points, and the AUC values were calculated using the linear trapezoidal rule. As insulin lispro and ultra-fast insulin/human soluble insulin were measured by different assays, no statistical analysis of concentration-dependent variables was made.

Differences in pharmacokinetic and pharmacodynamic variables were analysed (SAS version 8.02; SAS, Cary, NC, USA) using one-way ANOVA followed by a two-tailed Duncan’s multiple range test if the ANOVA showed significance.

Results

Blood glucose levels during the glucose clamps were comparable on the different study days as were their swings. Specifically, blood glucose levels were: 12 IU ultra-fast insulin 5.0 ± 0.2 mmol/l (coefficient of variation 4.7%); 12 U insulin lispro 5.0 ± 0.2 mmol/l (4.5%); 12 IU human soluble insulin 4.9 ± 0.4 mmol/l (7.7%); 6 IU ultra-fast insulin 4.9 ± 0.3 mmol/l (5.6%); and 3 IU ultra-fast insulin 4.9 ± 0.2 mmol/l (4.6%).

Pharmacokinetic variables obtained with 12 IU of ultra-fast insulin, insulin lispro and human soluble insulin Figure Figure1a1a shows that subcutaneous injection of ultra-fast insulin resulted in a more rapid increase in serum insulin than did injection of insulin lispro, which, in turn was more rapid than human soluble insulin. While the difference between ultra-fast insulin and human soluble insulin was significant, the difference between ultra-fast insulin and insulin lispro failed to reach statistical significance (tCmax) (Table 1, Fig. 1a). The decline of serum insulin levels was also more rapid with the ultra-fast insulin and insulin lispro. Figure Figure11 also shows a normalised graph comparing the different pharmacokinetic timing profiles. Serum C-peptide levels showed a moderate decline from baseline levels after s.c. injection of the different insulin formulations in all cases, including the different doses of ultra-fast insulin (data not shown).

Fig. 1
Mean serum insulin profiles (a), normalised mean serum insulin levels (b) and GIR profiles (c) after baseline correction obtained after s.c. application of 12 IU human soluble insulin (dotted lines), 12 U insulin lispro (bold lines) and 12 IU ultra-fast ...
Table 1
Pharmacokinetic and pharmacodynamic summary measures of 12 IU human soluble insulin, 12 U insulin lispro and 12, 6 and 3 IU ultra-fast insulin

Pharmacodynamic variables obtained with 12 IU of ultra-fast insulin, insulin lispro and human soluble insulin The pharmacodynamic properties of the three different insulin formulations mirror the pharmacokinetic variables. Subcutaneous injection of 12 IU ultra-fast insulin resulted in a time–action profile characterised by a more rapid rise in glucose consumption (tGIRmax+50%) in comparison to 12 U insulin lispro and 12 IU human soluble insulin (Table 1, Fig. 1b). Maximal metabolic activity (tGIRmax) was observed earlier and the metabolic activity in the first 2 h after s.c. injection (AUCGIR 0–120) was higher with ultra-fast insulin than with human soluble insulin, but failed to reach statistical significance when compared with insulin lispro. Maximal and total metabolic activity (GIRmax; AUCGIR) were comparable with all three insulin formulations.

Dose–response relationship The three dose levels of ultra-fast insulin (3, 6 and 12 IU) showed the expected linear and proportional increases in insulin absorption (Table 1, Fig. 1c). In addition, the decline of late half-maximal levels tended to be more rapid with 6 and 3 IU than with 12 IU. With an increase in dose from 3 to 6 IU the AUCINS 0–120 min increased by a factor of 1.92 and from 6 to 12 IU by a factor of 1.56. The respective factors in the AUCGIR 0–120 min were 1.37 and 1.27.

The time to early half-maximal action, time to maximal action and time to late half-maximal action after maximal action were comparable with all three ultra-fast insulin doses (Table 1, Fig. 1d). The maximal metabolic action and the AUCs were different with the three different doses.

Safety No serious adverse event occurred during this trial. Adverse events were headache and injection site pain, each reported by four participants (27%).

Discussion

This study showed that the ultra-fast insulin, VIAject, is absorbed and exerts its action faster than either s.c. injected human soluble insulin or insulin lispro at the same dose. Further, the maximal and overall metabolic effects of all three insulin formulations were comparable at the same dose level, showing that when human insulin is mixed with ingredients that lead the insulin molecule to maintain the monomeric state and simultaneously mask charges on its surface, an improvement in insulin absorption and action is achieved. These observations in healthy participants warrant confirmation in patients with diabetes.

The pharmacodynamic response seen in the first hours after s.c. administration of this novel human insulin formulation came closer to that of physiological prandial insulin secretion than do rapid-acting insulin analogues such as lispro. The time to early half-maximal action is 15 min faster with insulin lispro than with human soluble insulin; however, ultra-fast insulin is 32 min faster than human soluble insulin and 17 min faster than insulin lispro. It remains to be established by direct measurements whether this led to a stronger suppression of hepatic gluconeogenesis, which helps to optimise postprandial metabolic control [3].

During the first 2 h after administration, half the dose of ultra-fast insulin produced a metabolic effect in the same range as that achieved with 12 IU human soluble insulin and insulin lispro over that period. Appropriately designed clinical trials are now needed to establish whether it is possible to meet prandial insulin requirements as effectively with markedly reduced doses of ultra-fast insulin (vs other insulins). Clearly, several clinically relevant questions such as intra-individual variability of the metabolic activity of ultra-fast insulin and the effectiveness in controlling postprandial glycaemia remain to be studied [4, 5].

In conclusion, this novel human insulin formulation appears to be a promising candidate for improving postprandial glycaemic excursions with a reduced risk of late postprandial hypoglycaemia.

Acknowledgement

This study was supported by a research grant from Biodel.

Duality of interest S. Steiner is the inventor of VIAject and Chief Executive Officer of Biodel, Danbury, CT, USA. M. Hompesch is the Chief Executive Officer of Profil Institute for Clinical Research, San Diego, CA, USA. R. Pohl, P. Simms, F. Flacke and A. Pfützner are employees of Biodel and own shares/options. T. Mohr is an employee of IKFE, Mainz, Germany. L. Heinemann is a Chief Executive Officer of Profil, Neuss, Germany. M. Hompesch and L. Heinemann do not own shares/options in Biodel.

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Abbreviations

AUCGIR
AUC of glucose infusion rate
AUCINS
AUC of insulin concentration
Cmax
maximum insulin concentration
GIR
glucose infusion rate
GIRmax
maximum GIR
tCmax
time to Cmax
tGIRmax
time to GIRmax

References

1. Brange J, Owens DR, Kang S, Volund A (1990) Monomeric insulins and their experimental and clinical implications. Diabetes Care 13:923–954 [PubMed]
3. Bruttomesso D, Pianta A, Mari A et al (1999) Restoration of early rise in plasma insulin levels improves the glucose tolerance of type 2 diabetic patients. Diabetes 48:99–105 [PubMed]
4. Heise T, Nosek L, Stender A et al (2004) Lower within-subject variability of insulin detemir in comparison to NPH insulin and insulin glargine in people with type 1 diabetes. Diabetes 53:1614–1620 [PubMed]
5. Heinemann L, Heise T, Wahl LC et al (1996) Prandial glycaemia after a carbohydrate-rich meal in type I diabetic patients using the rapid acting insulin analogue [Lys(B28), Pro(B29)] human insulin. Diabet Med 13:625–629 [PubMed]

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